山东大学分子生物学相关资料.docx
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山东大学分子生物学相关资料
SectionA-Cellsandmacromolecules
1.Theglycosylationofsecretedproteinstakesplaceinthe...
Amitochondria.
Bperoxisomes.
Cendoplasmicreticulum.
Dnucleus.
2.Whichofthefollowingisanexampleofanucleoprotein?
Akeratin.
Bchromatin.
Chistone.
Dproteoglycan.
3.Whichofthefollowingisnotapolysaccharide?
Achitin.
Bamylopectin.
Cglycosaminoglycan.
Dglycerol.
4.Transmembraneproteins
Ajointwolipidbilayerstogether.
Bhaveintra-andextracellulardomains.
Carecontainedcompletelywithinthemembrane.
Dareeasilyremovedfromthemembrane.
SectionB-Proteinstructure
1.Whichofthefollowingisaniminoacid?
Aproline.
Bhydroxylysine.
Ctryptophan.
Dhistidine.
2.Proteinfamilymembersindifferentspeciesthatcarryoutthesamebiochemicalrolearedescribedas...
Aparalogs.
Bstructuralanalogs.
Cheterologs.
Dorthologs.
3.Whichofthefollowingisnotaproteinsecondarystructure?
Aα-helix.
Btriplehelix.
Cdoublehelix.
Dß-pleatedsheet.
4.Inisoelectricfocusing,proteinsareseparated.
AinapHgradient.
Binasaltgradient.
Cinadensitygradient.
Dinatemperaturegradient.
5.Edmandegradationsequencespeptides...
AusingacDNAsequence.
Baccordingtotheirmasses.
CFromtheC-terminustotheN-terminus.
DfromtheN-terminustotheC-terminus.
SectionC-propertiesofnucleicacids
1.Thesequence5'-AGTCTGACT-3'inDNAisequivalenttowhichsequenceinRNA?
A5'-AGUCUGUGACU-3'
B5'-UGTCTGUTC-3'
C5'-UCAGUCUGA-3'
D5'-AGUCAGACU-3'
2.WhichofthefollowingcorrectlydescribesA-DNA?
Aaright-handedantiparalleldoublehelixwith10bp/turnandbaseslyingperpendiculartothehelixaxis.
Baleft-handedantiparalleldouble-helixwith12bp/turnformedfromalternatingpyrimidine-purinesequences.
Caright-handedantiparalleldoublehelixwith11bp/turnandbasestiltedwithrespecttothehelixaxis.
Daglobularstructureformedbyshortintramolecularhelicesformedinasingle-strandnucleicacid.
3.DenaturationofdoublestrandedDNAinvolves.
Apreakageintoshortdouble-strandedfragments.
Bseparationintosinglestrands.
ChydrolysisoftheDNAbackbone.
Dcleavageofthebasesfromthesugar-phosphatebackbone.
4.Whichhasthehighestabsorptionperunitmassatawavelengthof260nm?
Adouble-strandedDNA.
Bmononucleotides.
CRNA.
Dprotein.
5.TypeIDNAtopoisomeraes...
Achangelinkingnumberby士2
BrequireATP.
CbreakonestrandofaDNAdoublehelix.
Darethetargetofantibacterialdrugs.
SectionD-Prokaryoticandeukaryoticchromatinstructure
1.Whichofthefollowingiscommonto·bothE.coliandeukaryoticchromosomes?
AtheDNAiscircular.
BtheDNAispackagedintonucleosomes.
CtheDNAiscontainedinthenucleus.
DtheDNAisnegativelysupercoiled.
2.Acomplof166bpofDNAwiththehistoneoctamerplushistoneHIisknownasa...
Anucleosomecore.
Bsolenoid.
C30nmfiber.
Dchromatosome.
3.Inwhatregionoftheinterphasechromosomedoestranscriptiontakeplace?
Athetelomere.
Bthecentromere.
Ceuchromatin.
Dheterochromatin.
4.WhichstatementaboutCpGislandsandmethylationisnottrue?
ACpGislandsareparticularlyresistanttoDNaseI.
BCpGmethylationisresponsibleforthemutationofCpGtoTpGineukaryotes.
CCpGislandsoccuraroundthepromotersofactivegenes.
DCpGmethylationisassociatedwithinactivechromatin.
5.Whichofthefollowingisanexampleofhighly-repetitiveDNA?
AAluelement.
Bhistonegenecluster.
CDNAminisatellites.
DdispersedrepetitiveDNA.
SectionE-DNAreplication
1.Thenumberofrepliconsinatypicalmammaliancellis...
A40-200.
B400.
C1000-2000.
D50000-100000.
2.Inprokaryotes,thelaggingstrandprimersareremovedby...
A3'to5'exonuclease.
BDNAligase.
CDNApolymeraseI.
DDNApolymeraseIII.
3.TheessentialinitiatorproteinattheE.colioriginofreplicationis...
ADnaA.
BDnaB.
CDnaC.
DDnaE.
4.WhichphasewouldacellenterifitwasstarvedofmitogensbeforetheRpoint?
AG1.
BS.
CG2.
DG0.
5.Whichoneofthefollowingstatementsistrue?
AoncethecellhaspassedtheRpoint,celldivisionisinevitable.
BthephosphorylationofRbbyaG1cyclin-CDKcomplexisacriticalrequirementforentryintoSphase.
CphosphorylationofE2FbyaG1cyclin-CDKcomplexisacriticalrequirementforentryintoSphase.
DcyclinD1andINK4p16aretumorsuppressorproteins.
6.Ineukaryotes,euchromatinreplicatespredominantly...
AinearlyS-phase.
BinmidS-phase.
CinlateS-phase.
DinG2-phase.
7.Prokaryoticplasmidscanreplicateinyeastcellsiftheycontainaclonedyeast...
AORC.
BCDK.
CARS.
DRNA.
SectionF-DNAdamage,repairandrecombination
1.Pernucleotideincorporated,thespontaneousmutationfrequencyinE.coliis...
A1in106.
B1in108.
C1in109.
D1in1010.
2.TheactionofhydroxylradicalsonDNAgeneratesasignificantamountof...
Apyrimidinedimmers.
B8-oxoguanine.
CO6-methylguanine.
D7-hydroxymethylguanine.
3.Inmethyl-directedmismatchrepairinE.coli,thedaughterstrandcontainingthemismatchedbaseisnickedby...
AMutHendonuclease.
BUvrABCendonuclease.
CAPendonuclease.
D3'to5'exonuclease.
4.Illegitimaterecombinationisanothernamefor...
Asite-specificrecombination.
Btransposition.
Chomologousrecombination.
DtranslesionDNAsynthesis.
5.TheexcisionrepairofUV-inducedDNAdamageisdefectiveinindividualssufferingfrom...
Ahereditarynonpolyposiscoloncancer.
BCrohn'sdisease.
Cclassicalxerodermapigmentosum.
Dxerodermapigmentosumvariant.
SectionG-Genemanipulation
1.Thepresenceofaplasmidinabacterialcultureisusuallydeterminedby...
Ablue-whitescreening.
Bgrowthinthepresenceofanantibiotic.
Carestrictionenzymedigest.
Dagarosegelelectrophoresis.
2.Theenzymealkalinephosphatase...
Athetake-upofaplasmidintoabacterium.
Btheexpressionofageneinabacterium.
Cthetake-upofabacteriophageintoabacterium.
Dtheisolationofaplasmidfromabacterium.
3.Transformationis...
Athetake-upofaplasmidintoabacterium.
Btheexpressionofageneinabacterium.、
Cthetake-upofabacteriophageintoabacterium.
Dtheisolationofaplasmidfromabacterium.
4.T4DNAligase...
ArequiresATP.
Bjoinsdouble-strandedDNAfragmentswithanadjacent3'-phosphateand5'-OH.
CrequiresNADH.
Djoinssingle-strandedDNA.
5.Inagarosegelelectrophoresis...
ADNAmigratestowardsthenegativeelectrode.
Bsupercoiledplamidsmigrateslowerthantheirnickedcounterparts.
Clargermoleculesmigratefasterthansmallermolecules.
DethidiumbromidecanbeusedtovisualizetheDNA.
SectionH-Cloningvectors
1.Blue-whiteselectionisused...
Atotestforthepresenceofaplasmidinbacteria.
BtorevealtheidentityofaclonedDNAfragment.
Ctoexpresstheproductofaclonedgene.
Dtotestforthepresenceofaclonedinsertinaplasmid.
2.Amultiplecloningsite...
Acontainsmanycopiesofaclonedgene.
Ballowsflexibilityinthechoiceofrestrictionenzymesforcloning.
Callowsflexibilityinthechoiceoforganismforcloning.
Dcontainsmanycopiesofthesamerestrictionenzymesite.
3.InfectionofE.colibybacteriophageλisnormallydetectedby...
Aresistanceofthebacteriatoanantibiotic.
Bthegrowthofsinglebacterialcoloniesonanagarplate.
Ctheappearanceofareasoflysedbacteriaonanagarplate.
DrestrictiondigestofthebacterialDNA.
4.Whichvectorwouldbemostappropriateforcloninga150kbfragmentofDNA?
Aaplasmid.
Baλvector.
CaBAC.
DaYAC.
5.Whichvectorwouldyouchqosetoexpressaforeigngeneinaplant?
Aabaculovirusvector.
Baretroviralvector.
CaYepvector.
DaT-DNAvector.
SectionI-Genelibrariesandscreening
1.Whichtwoofthefollowingstatementsaboutgenomiclibrariesarefalse?
AgenomiclibrariesaremadefromcDNA.
Bgenomiclibrariesmustberepresentativeiftheyaretocontainallthegenesinanorganism.
CgenomiclibrariesmustcontainaminimumnumberofrecombinantsiftheyaretocontainallthegenesInanorgamsm.
DtheDNAmustbefragmentedtoanappropriatesizeforthevectorthatisused.
EgenomiclibrariesmadefromeukaryoticDNAusuallyuseplasmidvectors.
2.WhichstatementcorrectlydescribessequentialstepsincDNAcloning?
AreversetranscriptionofMrnasecondstrandsynthesiscDNAendmodificationligationtovector.
BmRNApreparationcDNAsynthesisusingreversetranscriptasesecondstrandsynthesisusingterminaltransferase,ligationtovector.
CmRNAsynthesisusingRNApolymerasereversetranscriptionofmRNA,secondstrandsynthesis,ligationtovector.
DdoublestrandedcDNAsynthesisrestrictionenzymedigestionadditionoflinkersligationtovector.
3.Whichoneofthefollowingisnotavalidmethodofscreeningalibrary?
Ahybridizationofcolony/plaque-liftedDNAusinganucleicacidprobe.
Busingantibodiesraisedagainsttheproteinofinteresttoscreenanexpressionlibrary.
Cscreeningpoolsofclonesfromanexpressionlibraryforbiologicalactivity.
Dhybridizationofcolony/plaque-liftedDNAusinganantibodyprobe.
SectionJ-AnalysisandusesofclonedDNA
1.AlinearDNAfra