山东大学分子生物学相关资料.docx

1、山东大学分子生物学相关资料Section A - Cells and macromolecules 1The glycosylation of secreted proteins takes place in the . . .A mitochondria. B peroxisomes. C endoplasmic reticulum. D nucleus. 2Which of the following is an example of a nucleoprotein?A keratin. B chromatin. C histone. D proteoglycan.3Which of th

2、e following is not a polysaccharide? A chitin. B amylopectin. C glycosaminoglycan.D glycerol. 4. Transmembrane proteinsA join two lipid bilayers together. B have intra- and extracellular domains. C are contained completely within the membrane. D are easily removed from the membrane.Section B - Prote

3、in structure1. Which of the following is an imino acid? A proline. B hydroxy lysine. C tryptophan. D histidine. 2 Protein family members in different species that carry out the same biochemical role are described as . . . A paralogs. B structural analogs. C heterologs. D orthologs. 3. Which of the f

4、ollowing is not a protein secondary structure?A -helix.B triple helix. C double helix. D -pleated sheet. 4 In isoelectric focusing, proteins are separated .A in a pH gradient. B in a salt gradient. C in a density gradient. D in a temperature gradient.5Edman degradation sequences peptides . . . A usi

5、ng a cDNA sequence. B according to their masses. C From the C-terminus to the N-terminus. D from the N-terminus to the C-terminus. Section C - properties of nucleic acids1The sequence 5-AGTCTGACT-3 in DNA is equivalent to which sequence in RNA? A 5-AGUCUGUGACU -3 B 5 -UGTCTGUTC -3 C 5 -UCAGUCUGA-3 D

6、 5- AGUCAGACU-3 2. Which of the following correctly describes A-DNA? A a right-handed antiparallel double helix with 10 bp/turn and bases lying perpendicular to the helix axis. B a left-handed antiparallel double-helix with 12 bp/turn formed from alternating pyrimidine-purine sequences. C a right-ha

7、nded antiparallel double helix with 11 bp/turn and bases tilted with respect to the helix axis. D a globular structure formed by short intramolecular helices formed in a single-strand nucleic acid. 3. Denaturation of double stranded DNA involves.A preakage into short double-stranded fragments.B sepa

8、ration into single strands. C hydrolysis of the DNA backbone.D cleavage of the bases from the sugar-phosphate backbone. 4. Which has the highest absorption per unit mass at a wavelength of 260 nm?A double-stranded DNA. B mononucleotides. C RNA. D protein. 5. Type I DNA topoisomeraes .A change linkin

9、g number by士2 B require ATP. C break one strand of a DNA double helix. D are the target of antibacterial drugs. Section D - Prokaryotic and eukaryotic chromatin structure1Which of the following is common to both E. coli and eukaryotic chromosomes? A the DNA is circular. B the DNA is packaged into nu

10、cleosomes.C the DNA is contained in the nucleus. D the DNA is negatively supercoiled. 2A compl of 166 bp of DNA with the histone octamer plus histone HI is known as a . . . A nucleosome core. B solenoid. C 30 nm fiber. D chromatosome. 3In what region of the interphase chromosome does transcription t

11、ake place? A the telomere. B the centromere. C euchromatin. D heterochromatin. 4 Which statement about CpG islands and methylation is not true? A CpG islands are particularly resistant to DNase I. B CpG methylation is responsible for the mutation of CpG to TpG in eukaryotes. C CpG islands occur arou

12、nd the promoters of active genes. D CpG methylation is associated with inactive chromatin. 5Which of the following is an example of highly-repetitive DNA? A Alu element. B histone gene cluster. C DNA minisatellites. D dispersed repetitive DNA. Section E - DNA replication1The number of replicons in a

13、 typical mammalian cell is . . . A 40-200. B 400. C 1000-2000. D 50000-100000. 2. In prokaryotes,the lagging strand primers are removed by . . . A 3 to 5 exonuclease. B DNA ligase. C DNA polymerase I. D DNA polymerase III. 3. The essential initiator protein at the E. coli origin of replication is .

14、. . A DnaA. B DnaB. C DnaC. D DnaE. 4. Which phase would a cell enter if it was starved of mitogens before the R point? A G1. B S. C G2. D G0. 5. Which one of the following statements is true? A once the cell has passed the R point, cell division is inevitable. B the phosphorylation of Rb by a G1 cy

15、clin-CDK complex is a critical requirement for entry into S phase . C phosphorylation of E2F by a G1 cyclin-CDK complex is a critical requirement for entry into S phase. D cyclin D1 and INK4 p16 are tumor suppressor proteins. 6. In eukaryotes, euchromatin replicates predominantly. A in early S-phase

16、. B in mid S-phase. C in late S-phase. D in G2-phase. 7. Prokaryotic plasmids can replicate in yeast cells if they contain a cloned yeast. . . A ORC. B CDK. C ARS. D RNA. Section F - DNA damage, repair and recombination1. Per nucleotide incorporated, the spontaneous mutation frequency in E. coli is

17、. . . A 1 in 106. B 1 in 108. C 1 in 109. D 1 in 1010. 2. The action of hydroxyl radicals on DNA generates a significant amount of . . . A pyrimidine dimmers.B 8-oxoguanine.C O6- methylguanine. D 7-hydroxymethylguanine.3. In methyl-directed mismatch repair in E. coli, the daughter strand containing

18、the mismatched base is nicked by . . . A MutH endonuclease.B UvrABC endonuclease.C AP endonuclease.D 3 to 5 exonuclease.4. Illegitimate recombination is another name for . . . A site-specific recombination. B transposition.C homologous recombination.D translesion DNA synthesis. 5. The excision repai

19、r of UV-induced DNA damage is defective in individuals suffering from . A hereditary nonpolyposis colon cancer. B Crohns disease. C classical xeroderma pigmentosum. D xeroderma pigmentosum variant. Section G - Gene manipulation1. The presence of a plasmid in a bacterial culture is usually determined

20、 by . . . A blue-white screening. B growth in the presence of an antibiotic. C a restriction enzyme digest. D agarose gel electrophoresis. 2. The enzyme alkaline phosphatase. . . A the take-up of a plasmid into a bacterium. B the expression of a gene in a bacterium.C the take-up of a bacteriophage i

21、nto a bacterium.D the isolation of a plasmid from a bacterium. 3. Transformation is . . . A the take-up of a plasmid into a bacterium. B the expression of a gene in a bacterium.、 C the take-up of a bacteriophage into a bacterium. D the isolation of a plasmid from a bacterium. 4. T4 DNA ligase . . .

22、A requires ATP. B joins double-stranded DNA fragments with an adjacent 3-phosphate and 5-OH. C requires NADH. D joins single-stranded DNA.5. In agarose gel electrophoresis . . . A DNA migrates towards the negative electrode. B supercoiled plamids migrate slower than their nicked counterparts. C larg

23、er molecules migrate faster than smaller molecules.D ethidium bromide can be used to visualize the DNA. Section H - Cloning vectors 1. Blue-white selection is used. . . A to test for the presence of a plasmid in bacteria. B to reveal the identity of a cloned DNA fragment. C to express the product of

24、 a cloned gene. D to test for the presence of a cloned insert in a plasmid. 2. A multiple cloning site . . . A contains many copies of a cloned gene. B allows flexibility in the choice of restriction enzymes for cloning. C allows flexibility in the choice of organism for cloning. D contains many cop

25、ies of the same restriction enzyme site. 3. Infection of E. coli by bacteriophage is normally detected by . . . A resistance of the bacteria to an antibiotic. B the growth of single bacterial colonies on an agar plate. C the appearance of areas of lysed bacteria on an agar plate. D restriction diges

26、t of the bacterial DNA. 4. Which vector would be most appropriate for cloning a 150 kb fragment of DNA? A a plasmid. B a vector. C a BAC. D a YAC. 5. Which vector would you chqose to express a foreign gene in a plant? A a baculovirus vector. B a retroviral vector. C a Yep vector. D a T-DNA vector. S

27、ection I - Gene libraries and screening 1. Which two of the following statements about genomic libraries are false? A genomic libraries are made from cDNA. B genomic libraries must be representative if they are to contain all the genes in an organism. C genomic libraries must contain a minimum numbe

28、r of recombinants if they are to contain all the genes In an orgamsm. D the DNA must be fragmented to an appropriate size for the vector that is used. E genomic libraries made from eukaryotic DNA usually use plasmid vectors. 2. Which statement correctly describes sequential steps in cDNA cloning?A r

29、everse transcription of Mrna second strand synthesis cDNA end modification ligation to vector. B mRNA preparation cDNA synthesis using reverse transcriptase second strand synthesis using terminal transferase, ligation to vector. C mRNA synthesis using RNA polymerase reverse transcription of mRNA, se

30、cond strand synthesis, ligation to vector. D double stranded cDNA synthesis restriction enzyme digestion addition of linkers ligation to vector. 3. Which one of the following is not a valid method of screening a library? A hybridization of colony / plaque-lifted DNA using a nucleic acid probe. B using antibodies raised against the protein of interest to screen an expression library. C screening pools of clones from an expression library for biological activity. D hybridization of colony/plaque-lifted DNA using an antibody probe. Section J - Analysis and uses of cloned DNA 1. A linear DNA fra