8 常用载体通用引物.docx
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8常用载体通用引物
载体名称
上游引物(5'primer)
下游引物(3'primer)
P3*FLAG-CMV
CMV-30(825-854)
CMV-24(1129-1152)
pAc5.1/V5-His(_A,_B,_C)
pAc5-5
BGHrev
pAcG2T
pGEX-5
notavailable
pACT
T7EEV
T3/EBVrev
pACT2
GAL4ADfor
pGAL4-ADrv
pACT2
p17110
p12584
pACYC184(BamHI-site)
PBRforBam
PBRrevBam
pACYCDuet-1(MCSI)
ACYCDuetUP1Primer
DuetDOWN1
pACYCDuet-1(MCSII)
DuetUp2
T7-Terminator
pAdEasy-1
notavailable
notavailable
pAdTrack-CMV
notavailable
notavailable
pAS2-1
GAL4Bdfor/P24990
pGAL4-BDrv/P31430
pB42AD
pB42ADF
PB42ADR
pBABE
pBMN_5'
pBacPAK8
BAC1
BAC2
pBAD
pBAD-F
pBAD-R
pBAD/HisABC
pBAD-5'
pBBR1MCS
T3/M13rev
T7/M13for
pBD-GAL4
pBD-GAL4-F
PBD-GAL4-R
pBI121
35S
NOS
pBIND
GAL4-Bdfor
T3,EBVrev
pBK-CMV
M13F/T7
M13R/T3
pBluescriptIIKS(+/-)
T3/M13rev
T7/M13for(优先用M13F)
pBluescriptIISK(+/-)
T3/M13rev
T7/M13for
pBluescriptKS(+/-)
T3/M13rev
T7/M13for
pBluescriptSK(+/-)
T3/M13rev
T7/M13for
pBR322(BamHI-site)
PBRforBam
PBRrevBam
pBR322(EcoRI/HindIII-sites)
pBRforEco
pBRrevHind
pBR325(BamHI-site)
PBRforBam
PBRrevBam
pBR329(BamHI-site)
PBRforBam
PBRrevBam
pBridge(MCSI)
GAL4-Bdfor
3'BD
pBridge(MCSII)
notavailable
notavailable
pBS-TII
T3/M13rev
T7/M13for
pBS(AMP)
T7
T3
pBSR
T3
T7ter
pBudCE4.1(MCSI)
CMV-Profor,T7
c-mycrev,EBVrev
pBudCE4.1(MCSII)
EF-1αFwd
BGH
pBudCE4.1/lacZ/CAT
CMV-Profor,T7
c-mycrev,EBVrev
pBV220
pBV220F
PBV220R
pCAL-c
T7/Tac-Profor
T7-Terminator
pCAL-n
T7/Tac-Profor
T7-Terminator
pCAMBIA1301(1300)
pCAMBIA1301-5'(ECORI)/M13R
pCAMBIA1301-3'(HindIII)
pCAMBIA1304
pCAMBIA1301-5'(ECORI)
pCAMBIA1301-3'(HindIII)
pCAMBIA2300
M13R(-48)
M13F(-47)/M13F(-49)
pCANTB5E
S1
S6
pCAT3-enhancer
RVP3
此载体无反向引物
pCDFDuet-1(MCSI)
ACYCDuetUP1Primer
DuetDOWN1
pCDFDuet-1(MCSII)
DuetUP2
T7TER
pcDNA1.1
CMV-Profor,T7
pCDM8-3
pcDNA2.1
M13for/T7
M13rev/Tac-Profor
pcDNA3
T7/CMV-Profor
BGHrev/SP6
pcDNA3.1(+/-)
pcDNA3.1F/T7/CMV-Profor
BGHrev
pcDNA3.1/His(_A,_B,_C)
T7/CMV-Profor
BGHrev
pcDNA3.1/myc-His(_A,_B,_C)
T7/CMV-Profor
BGHrev/c-mycrev
pcDNA3.1/V5-His(_A,_B,_C)
T7/CMV-Profor
BGHrev/V5rev
pcDNA3.1/Hygro(+,-)
T7/CMV-Profor
BGHrev/V5rev
pcDNA3.1/Zeo(+.-)
T7
BGHrev
pcDNA3.3TOPOTA
CMV-Profor
TKPAreverse
pcDNA4/HisMax(_A,_B,_C)
Xpressfor
BGHrev
pcDNA4/HisMax-TOPO
Xpressfor
BGHrev
pcDNA4/HisMax-TOPO/lacZ
Xpressfor
BGHrev
pcDNA4/myc-His(_A,_B,_C)
T7,CMV-Profor
BGHrev,c-mycrev
pcDNA4/TO
TREfor/CMV-Profor
BGHrev
pcDNA4/V5-His(_A,_B,_C)
CMV-Profor,T7
BGHrev,V5rev
pcDNA4-TET/ON/AMP+
CMV-Profor
pcDNA5/FRT
T7/CMV-Profor
BGHrev
pcDNA6/myc-His(_A,_B,_C)
CMV-Profor,T7
BGHrev,c-mycrev
pcDNA6/V5-His(_A,_B,_C)
CMV-Profor,T7
BGHrev,V5rev
pCEP4
pCEP-F/CMV-Profor
EBVrev
pCI
T7(17BASE)
EBVrev
pCI-neo
T7(17BASE)
EBVrev,T3
pCMS-EGFP
T7
T3
pCMS-EGFP
T7
EBVrev,T3
pCMV-3Tag-4A
T3/PFLAG-CMV-F
T7
pCMV5
pCMV5F
PCMV5R
pCMV6
CMV-Profor
pCMV6-3、HGHrev
pCMV6-XL4
CMV-Profor/T7
pCMV6-3、HGHrev
pCMV-HA
PCMV-F,CMV-Profor,TREfor
PCMV-R,EBVrev
pCMV-MYC(AMP+)
PCMV-F
PCMV-R, EBVrev
pCMVmyc/nuc
CMV-Profor
BGHrev,c-mycrev
pcmv-scriptEXVector
T3
T7
pCMV-Sport6
T7/M13for
SP6/M13rev
pCOLADuet-1(MCSI)
ACYCDuetUP1Primer
DuetDOWN1
pCOLADuet-1(MCSII)
DuetUP2
T7TER
pCR2.1
T7,M13for
M13rev
pCR2.1-TOPO
M13for,T7
M13rev
pCR3.1
T7
BGHrev
pCR3.1-Uni
T7
BGHrev
pCR4
T3/M13rev
T7/M13for
pCR4-Blunt
T3/M13rev
T7/M13for
pCR4-TOPO
M13rev,T3
M13for,T7
pCR-Blunt
M13rev
T7,M13for
pCR-BluntII
M13rev/SP6
T7/M13for
pCR-BluntII-TOPO
M13rev/SP6
M13for/T7
pCRII
M13rev/SP6
T7/M13for
pCRII-TOPO
M13rev/SP6
M13for/T7
pCR-ScriptSK(+)
T7/M13for
T3/M13rev
pCR-XL-TOPO
M13rev
T7,M13for
pCS2+(MCSI)
SP6
EBVrev,T7?
pCS2+(MCSII)
T3
pSG5-3
pDB_GAL4cam
T7P
pDB_leu
PDBLeu-5
PDBLeu-3
pDEST22
DEST22F
DEST22R
pDEST15
pGEX-5
T7-Terminator
pDEST20
pGEX-5
pFastBac-3
pDEST26
TREfor/CMV-Profor
T7/M13for
pDisplay
T7/CMV-Profor
c-mycrev
pDNR-LIB(MCS_A)
M13for,T7
pSG5-3,M13R
pDonar
M13F
M13R
pDONR201
PDONR-F
PDONR-R
pDONR207
PDONR-F
PDONR-R
pDONR223
M13F
M13R
pDrive
T7/M13rev
SP6/M13for
pDsRED1-C1
(DsRed1-N-f)
DsRed1-C-r
pDsRed1-N1
CMV-Profor
RFP-Nrev,CMV-R?
pDsRed2-N1
(DsRed1-N-f)
DsRed1-C-r
pDsRed2-C1
(DsRed1-N-f)
DsRed1-C-r
pDsRED2-C1(KAN+)
pDsRED-ex-C1-F
pEGFP-N-3’
pDsRed-Express
dsRed1_N_primer/CMV-F
dsRed1_C_primer/CMV-R
pDSRED-monomer-N1(KAN+)
dsRed1_N_primer
pDSRED-N1(KAN+)
pEGFP-N-5’
pDSRED-N-R
pEASY-Blunt
M13F
T7/M13R
pEASY-BluntSimple
M13F
T7/M13R(距离插入位点合适,优先安排)
pEASY-T1Simple
M13F
M13R(距离插入位点合适,优先安排)
pEASY-T1
M13F/T7
M13R
pEASY-T3
M13F/T7
M13R/SP6
pECFP-N(C)
PECFP-N-5.
PECFP-N-3,
pEF/myc/cyto/ER/mito/nuc
pEF-5
Pcdna3.1R/BGHrev
pEF1(4,6/myc-His)
T7/pEF-5
Pcdna3.1R/BGHrev
pEF1(4,6/V5-His)
T7/pEF-5
Pcdna3.1R/BGHrev
pEF1(4;6/His_A;_B;_C)
T7/Xpressfor
Pcdna3.1R/BGHrev
pEF5/FRT/V5-D(-TOPO)
T7/pEF-5
Pcdna3.1R/BGHrev
pEGFP
M13rev
EGFP-Nrev
pEGFP-1
notavailable
EGFP-Nrev/PEGFP-N-3′
pEGFP-C
PEGFP-C-5′
PEGFP-C-3′
pEGFP-C1
EGFP-Cfor
SV40-pArev
pEGFP-C2
EGFP-Cfor
SV40-pArev
pEGFP-C3
EGFP-Cfor
SV40-pArev
pEGFP-F
EGFP-Cfor
SV40-pArev
pEGFP-N
PEGFP-N-5′
PEGFP-N-3′
pEGFP-N1
CMV-Profor
EGFP-Nrev
pEGFP-N2
CMV-Profor
EGFP-Nrev
pEGFP-N3
CMV-Profor
EGFP-Nrev
pENTR
M13F
M13R
pET-11(-a,-b,-c,-d)
T7
T7-Terminator
pET-12(-a,-b,-c)
T7
T7-Terminator
pET-14b
T7
T7-Terminator
pET-15b
T7
T7-Terminator
pET-16b
T7
T7-Terminator
pET-17b
T7
T7-Terminator
pET-17xb
T7
T7-Terminator
pET-19b
T7
T7-Terminator
pET-20b(+)
T7
T7-Terminator
pET-21(-a,-b,-c,-d)(+)
T7
T7-Terminator
pET-22b(+)
T7
T7-Terminator
pET-23(-a,-b,-c,-d)(+)
T7
T7-Terminator
pET-24(-a,-b,-c,-d)(+)
T7
T7-Terminator
pET-25b(+)
T7
T7-Terminator
pET-26b(+)
T7
T7-Terminator
pET-27b(+)
T7
T7-Terminator
pET-28a(-b,-c)(+)
T7
T7-Terminator
pET-29a(-b,-c)(+)
S.tag/T7
T7-Terminator
pET-3(-a,-b,-c,-d)
T7
T7-Terminator
pET-30_Ek/LIC
T7
T7-Terminator
pET-30_Xa/LIC
T7
T7-Terminator
pET-30a(-b,-c)(+)
S.tag/T7
T7-Terminator
pET-31b(+)
notavailable
T7-Terminator
pET-32_Ek/LIC
TRXfor
T7-Terminator
pET-32_XA/LIC
TRXfor
T7-Terminator
pET-32a(-b,-c)(+)
S.tag/TRXfor
T7-Terminator
pET-33b(+)
T7
T7-Terminator
pET-34b(+)
S.tag
T7-Terminator
pET-35b(+)
S.tag
T7-Terminator
pET-36b(+)
S.tag
T7-Terminator
pET-37b(+)
S.tag
T7-Terminator
pET-38b(+)
notavailable
T7-Terminator
pET-39b(+)
S.tag
T7-Terminator
pET-40b(+)
S.tag
T7-Terminator
pET-41_Ek/LIC
GST-Cfor
T7-Terminator
pET-41a(-b,-c)(+)
S.tag/pGEX-5
T7-Terminator
pET-42a(-b,-c)(+)
S.tag/pGEX-5
T7-Terminator
pET-43.1a(-b,-c)(+)
S.tag
coliDOWN
pET-43.1a_EK/LIC
S.tag
notavailable
pET-44a(-b,-c)(+)
S.tag
T7-Terminator
pET-44a_EK/LIC
S.tag
notavailable
pET-45b(+)
T7
T7-Terminator
pET-46_Ek/LIC
T7
T7-Terminator
pET-5(-a,-b,-c)
T7
T7-Terminator
pET-52b(+)
T7
T7-Terminator
pET-7
T7
T7-Terminator
pET-9(-a,-b,-c,-d)
T7
T7-Terminator
pETcoco-1
T7
T7-Terminator
pETDuet-1(MCSI)
pETUP1
DuetDOWN1
pETDuet-1(MCSII)
DuetUp2
T7-Terminator
pET-T4
T7
T7-Terminator
pEYFP-C1
pEGFP-C-5’
pEGFP-C-3'
pEYFP-N1
pEGFP-N-5’,CMV-Profor
pEGFP-N-3’
pFLAG-ATS
N26for
pTrcHis-rv;C24rev
pFLAG-CTC
N26for
pTrcHis-rv;C24rev
pFLAG1
N26for
pTrcHis-rv;C24rev
pFLAG-CMV(-2)
CMV-30;CMV-Profor
CMV-24
pFLAG-CMV1
CMV-30
CMV-24
pGAD424
GAL4_ADfor
3'AD
pGADGH
GAL4_ADfor
3'AD
pGADT7
T7/GAL4_ADfor
3'AD
pGADT7-Rec
T7/GAL4_ADfor
3'AD
pGADT-T
T7
3,BD
pGAD10
GAL4_ADfor
3'AD
pGAPZa-A
a-FACTOR
3'AOX
pGBKT7
T7
3,BD
pGBT9
MATCHMAKER5'DNA-BD/GAL4BD
MATCHMAKER3'DNA-BD
pGEM-1
T7
SP6
pGEM-3
T7
SP6
pGEM-3Zf(+)(-)
SP6/M13rev
T7/M13for
pGEM-4
T7
SP6
pGEM-4Z
T7/M13for
SP6/M13rev
pGEM-5Zf(+)(-)
SP6/M13rev
T7/M13for
pGEM-7Zf(+)(-)
SP6/M13rev
T7/M13for
pGEM-9Zf(+)(-)
T7/M13for
SP6/M13rev
pGEM-T
T7/M13for
SP6/M13rev
pGEM-T_Easy
T7/M13for
SP6/M13rev
pGene/V5-His_A(_B,_C)
pGENE-5
BGHrev,V5rev
pGEX-2T
pGEX-5
pGEX-3
pGEX-2TK
pGEX-5
pGEX-3
pGEX-3X
pGEX-5
pGEX-3
pGEX-4T-1(-2,-3)
pGEX-5
pGEX-3
pGEX-5X-1(-2,-3)
pGEX-5
pGEX-3
pGEX-6P-1(-2,-3)
pGEX-5
pGEX-3
pGL2
GLP1
GLP2
pGL3
RVP3
GLP2(RVP4)(没有特别说明用GLP2)
pGL3-Basic(MCSI)
RVP3(PGL3+)?
GLP2(PGL3-)?
pGL3-Basic(MCSII)
RVP4,
PLXSN-3'?
pGL4
pGlow(-TOPO)
T7
pGlow-3
pGM-T
T7
SP6
pGWC
SP6
T7
pHook-2
T7/CMV-Profor
notavailable
pIND/V5-His_A(_B,_C)
notavailable
BGHrev,V5rev
PinpointTMVector
PinpointPrimer
SP6
pIRES(MCSI)
T7
pIRES-3
pIRES(MCSII)
notavailable
T3,EBVrev
pIRES2-EGFP
CMV-Profor,pIRES2-EGFP.P5’
IRESrev,pIRES2-EGFP.P3’
pIRES-hrGFP-2a
CMV-Profor,T3
pIRES-hrGFP-3
pIRESneo2
T7/CMV-Profor
pIRES-3
pIVEX(all)
T7/pBRrevBam
T7-Terminator
pLEXA
pLEXA-F
pLEXA-R
pLNCX
pLNCX-F
pLNCX-R
pLNCX2
CMV-Profor
pRevTRE-3
pLVTHM(H1启动子下游)
H1primer
SP6
pLXSN
pLXSN-F
pLXSN-R
pMALc
MalEprimer
M13F(-47)
pMAL-c2E
MalEprimer
M13F(-47)
pMAL-C2x
Pmal-c2x-F(P5’)
Pmal-C2X-R(P3’)
pMALd
MalEprimer
M13F(-47)
pMALe
MalEprimer
M13F(-47)
pMAL-P4X
MalEprimer
M13F(-47)
pMAL-p2X
MalEprimer
M13F(-47)
pMETA/B/C
AUG1F
AUG1R
pMD18-T
M13R(-48)ECORI
M13F(-47)(HINDⅢ)
pMD19-T
M13F47
M13R48
pMD20-T
M13F47
M13R48
pMOSBlue
T7/M13rev
M13for
pMSCV-puro
pLXSNfw