精品荧光染料激发和发射光谱表.docx

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精品荧光染料激发和发射光谱表.docx

精品荧光染料激发和发射光谱表

荧光素名称

激发波长(nm)

发射波长(nm)

备注

1,5IAEDANS

336

490

 

1,8-ANS

372

480

 

4-Methylumbelliferone

385

502

 

5-carboxy-2,7-dichlorofluorescein

504

529

 

5-Carboxyfluorescein(5-FAM)

492

518

 

5-Carboxynapthofluorescein(pH10)

512/598

563/668

RatioDye,pH

5-Carboxytetramethylrhodamine(5-TAMRA)

542

568

 

5-FAM(5-Carboxyfluorescein)

492

518

 

5-HAT(HydroxyTryptamine)

370-415

520-540

 

5-HydroxyTryptamine(HAT)

370-415

520-540

 

5-ROX(carboxy-X-rhodamine)

578

567

604

591

 

5-TAMRA(5-Carboxytetramethylrhodamine)

548

542

552

568

 

6-Carboxyrhodamine6G

518

543

 

6-CR6G

518

543

 

6-JOE

520

548

 

7-Amino-4-methylcoumarin

351

430

 

7-AminoactinomycinD(7-AAD)

546

647

 

7-Hydroxy-4-methylcoumarin

360

449,455

 

9-Amino-6-chloro-2-methoxyacridine

412,430

471,474

 

ABQ

344

445

 

AcidFuchsin

540

630

 

ACMA(9-Amino-6-chloro-2-methoxyacridine)

412,430

471,474

 

AcridineOrange+DNA

502

526

 

AcridineOrange+RNA

460

650

 

AcridineOrange,bothDNA&RNA

440-480

520-650

 

AcridineRed

455-600

560-680

 

AcridineYellow

470

550

 

Acriflavin

436

520

 

AcriflavinFeulgenSITSA

355-425

460

 

Aequorin(Photoprotein)

 

466

Photoprotein

AFPs-AutoFluorescentProtein-(Quantum

 

 

 

Biotechnologies)seesgGFP,sgBFP

AlexaFluor350™

346

342

442

441

 

AlexaFluor430™

431

540

 

AlexaFluor488™

495,492

519,520

 

AlexaFluor532™

531,532

553,554

 

AlexaFluor546™

556,557

572,573

 

AlexaFluor568™

577,578

603

 

AlexaFluor594™

590,594

617,618

 

AlexaFluor633™

632

650

 

AlexaFluor647™

647

666

 

AlexaFluor660™

668

698

 

AlexaFluor680™

679

702

 

AlizarinComplexon

530-560,580

580,624-645

 

AlizarinRed

530-560

580

 

Allophycocyanin(APC)

630,645

655,660

 

AMC,AMCA-S

345

445

 

AMCA(Aminomethylcoumarin)

345

347

425

444

 

AMCA-X

353

442

 

AminoactinomycinD

555

655

 

Aminocoumarin

346

350

442

445

 

Aminomethylcoumarin(AMCA)

345

347

425

444

 

AnilinBlue

 

600

 

Anthrocylstearate

360-381

446

 

APC(Allophycocyanin)

630,645

655,660

 

APC-Cy7

625-650

755

 

APTRA-BTC=RatioDye,Zn2+

466/380

520/530

RatioDye,Zn2+

APTS

424

505

 

AstrazonBrilliantRed4G

500

585

 

AstrazonOrangeR

470

540

 

AstrazonRed6B

520

595

 

AstrazonYellow7GLL

450

480

 

Atabrine

436

490

 

ATTO-TAG™CBQCA

465

560

 

ATTO-TAG™FQ

486

591

 

Auramine

460

550

 

AurophosphineG

450

580

 

Aurophosphine

450-490

515

 

BAO9(Bisaminophenyloxadiazole)

365

395

 

BCECF(highpH)

492,503

520,528

 

BCECF(lowpH)

482

520

 

BerberineSulphate

430

550

 

BetaLactamase

409

447,520

 

BFPblueshiftedGFP(Y66H)

BlueFluorescentProtein

381,382,383

445,447,448

blueshiftedGFP(Y66H)

BlueFluorescentProtein

BFP/GFPFRET

 

 

 

Bimane

398

490

 

Bisbenzamide

360

461

 

Bisbenzimide(Hoechst)

360

461

 

bis-BTC=RatioDye,Zn2+

455/405

529/505

RatioDye,Zn2+

BlancophorFFG

390

470

 

BlancophorSV

370

435

 

BOBO™-1

462

481

 

BOBO™-3

570

602

 

Bodipy492/515

490

515

 

Bodipy493/503

533

549

 

Bodipy500/510

509

515

 

Bodipy505/515

502

510

 

Bodipy530/550

528

547

 

Bodipy542/563

543

563

 

Bodipy558/568

558

569

 

Bodipy564/570

564

570

 

Bodipy576/589

579

590

 

Bodipy581/591

584

592

 

Bodipy630/650-X

625

642

 

Bodipy650/665-X

647

665

 

Bodipy665/676

605

676

 

BodipyFl

504,505

511,

 

513

BodipyFLATP

505

514

 

BodipyFl-Ceramide

505

511

 

BodipyR6GSE

528

547

 

BodipyTMR

542

574

 

BodipyTMR-Xconjugate

544

573

 

BodipyTMR-X,SE

544

570

 

BodipyTR

589

617

 

BodipyTRATP

591

620

 

BodipyTR-XSE

588

616

 

BO-PRO™-1

462

481

 

BO-PRO™-3

544

570

 

BrilliantSulphoflavinFF

430

520

 

BTC-RatioDyeCa2+

464/401

533/529

RatioDyeCa2+

BTC-5N-atioDye,Zn2+

459/417

517/532

RatioDye,Zn2+

Calcein

494

517

 

CalceinBlue

373

440

 

CalciumCrimson™

588,589

611,615

 

CalciumGreen

501,506

531

 

CalciumGreen-1Ca2+Dye

506

531

Ca2+Dye

CalciumGreen-2Ca2+

506/503

536

Ca2+

CalciumGreen-5NCa2+

506

532

Ca2+

CalciumGreen-C18Ca2+

509

530

Ca2+

CalciumOrange

549

575

576

 

CalcofluorWhite

385,395,405

437,440,445

 

Carboxy-X-rhodamine(5-ROX)

576

601

 

CascadeBlue™

377

398

399

420

423

 

CascadeYellow

399

400

550

552

 

Catecholamine

410

470

 

CCF2(GeneBlazer)

 

 

 

CFDA

494

520

 

CFP-CyanFluorescentProtein

430,433,436,

474,475,476,

CyanFluorescentProtein

(453)

(501)

CFP/YFPFRET

 

 

 

Chlorophyll

480

650

 

ChromomycinA

436-460

470

 

ChromomycinA

445

575

 

CL-NERF(RatioDye,pH)

504/514

540

RatioDye,pH

CMFDA

494

520

 

CoelenterazineCa2+Dye,bioluminescence

(429)

465

Ca2+Dye,bioluminescence,

nativemolecule

Coelenterazinecp(Ca2+Dye,)

(430)

442

Ca2+Dye,bioluminescence

Coelenterazinef

(437)

473

Ca2+Dye,bioluminescence

Coelenterazinefcp

 

452

Ca2+Dye,bioluminescence

Coelenterazineh

(437)

464

Ca2+Dye,bioluminescence

Coelenterazinehcp

(433)

444

Ca2+Dye,bioluminescence

Coelenterazineip

 

441

Ca2+Dye,bioluminescence

Coelenterazinen

(431)

467

Ca2+Dye,bioluminescence

CoelenterazineO

460

575

 

CoumarinPhalloidin

387

470

 

C-phycocyanine

 

 

 

CPMMethylcoumarin

384

469

Methylcoumarin

CTC

400-450

602

 

CTCFormazan

 

 

 

Cy2™

489

506

 

Cy3.18

554

568

 

Cy3.5™

581

598

 

Cy3™

514

552

554

566

570

 

Cy5.18

649

666

 

Cy5.5™

675

695

 

Cy5™

649

666

670

 

Cy7™

710,743

767,805

 

CyanGFP

433(453)

475(501)

 

cyclicAMPFluorosensor(FiCRhR)

500

517

 

CyQuantCellProliferationAssay

480

520

CellProliferationAssay

Dabcyl

453

 

 

Dansyl

340

578

 

DansylAmine

337

517

 

DansylCadaverine

335

518

 

DansylChloride

372

518

 

DansylDHPE

336

517

 

Dansylfluoride

356

none

 

DAPI

359

461

 

Dapoxyl

403

580

 

Dapoxyl2

374

574

 

Dapoxyl3

373

574

 

DCFDA

504

529

 

DCFH(DichlorodihydrofluoresceinDiacetate)

505

535

 

DDAO

463

607

 

DHR(Dihydorhodamine123)

505

534

 

Di-4-ANEPPS

496

705

 

Di-8-ANEPPS(non-ratio)

488

498

605

713

 

DiA(4-Di-16-ASP)

456

591

 

DichlorodihydrofluoresceinDiacetate(DCFH)

505

535

 

DiD-LipophilicTracer

644

665

LipophilicTracer

DiD(DiIC18(5))

644

665

 

DIDS

341

415

 

Dihydorhodamine123(DHR)

505

535

 

DiI(DiIC18(3))

549,551

565

 

Dinitrophenol

349

 

 

DiO(DiOC18(3))

484,487

501,502

 

DiR

748

780

LipophilicTracer

DiR(DiIC18(7))

750

779

 

DM-NERF(highpH)

497/510

540

RatioDye,pH

DNP

349

 

 

Dopamine

340

 

490-520

DsRed

558

583

Redfluorescentprotein

DTAF

494

520

 

DY-630-NHS

621

660

Hemicyane

labelforproteinsandDNA

DY-635-NHS

634

664

Hemicyane

labelforproteinsandDNA

EBFP

383

447

EnhancedBlueFluorescentProtein

ECFP

436

474

EnhancedCyanFluorescentProtein

EGFP

488,498

507,516

EnhancedGreenFluorescentProtein

ELF97

345

530

 

Eosin

524

545

 

Erythrosin

529,532

554,555

 

ErythrosinITC

529

555

 

EthidiumBromide

510,523

595,605

 

Ethidiumhomodimer-1(EthD-1)

528

617

 

Euchrysin

430

540

 

EukoLight

 

 

 

Europium(III)chloride

 

 

 

EYFP

513,520

527,532

EnhancedYellowFluorescentProtein

FastBlue

360

440

 

FDA

494

520

 

Feulgen(Pararosaniline)

570

625

 

FIF(FormaldehydInducedFluorescence)

405

433

 

FITC

490,494

520,525

 

FITCAntibody

493

517

 

FlazoOrange

375-530

612

 

Fluo-3

480–506,506

520,527

 

Fluo-4

494

516

 

Fluorescein(FITC)

490,494

520,525

 

FluoresceinDiacetate

494

520

 

Fluoro-Emerald

495

524

 

Fluoro-Gold(Hydroxystilbamidine)

361

536

 

Fluor-Ruby

555

582

 

FluorX

494

520

 

FM1-43™

479

598

 

FM4-46

515

640

 

FuraRed™(highpH)

572

657

 

FuraRed™/Fluo-3

 

 

 

Fura-2,highcalcium

335

505

Excitationratiodye

Fura-2,lowcalcium

363

512

Excitationratiodye

Fura-2/BCECF

 

 

 

GenacrylBrilliantRedB

520

590

 

GenacrylBrilliantYellow10GF

430

485

 

GenacrylPink3G

470

583

 

GenacrylYellow5GF

430

475

 

GeneBlazer(CCF2)

 

 

 

GFP(S65T)

498

516

 

GFPredshifted(rsGFP)

498

516

 

GFPwildtype,non-UVexcitation(wtGFP)

475

509

 

GFPwildtype,UVexcitation(wtGFP)

395

509

 

GFPuv

385

508

 

GloxalicAcid

405

460

 

GranularBlue

355

425

 

Haematoporphyrin

530-560

580

 

Hoechst33258

345

487

 

Hoechst33342

347

483

 

Hoechst34580

392

440

 

HPTS

355

465

 

Hydroxycoumarin

325-360

386-455

 

Hydroxystilbamidine(FluoroGold)

361

536

 

Hydroxytryptamine

400

530

 

Indo-1,highcalcium

330

401

Emissionratiodye

Indo-1,lowcalcium

346

475

Emissionratiodye

Indodicarbocyanine(DiD)

644

665

 

Indotricarbocyanine(DiR)

748

780

 

IntrawhiteCf

360

430

 

JC-1

514

529

 

JO-JO-1

530

545

 

JO-PRO-1

532

544

 

LaserPro

795

812

 

Laurodan

355

460

 

LDS751(DNA)

543

712

 

LDS751(RNA)

590

607

 

LeucophorPAF

370

430

 

LeucophorSF

380

465

 

LeucophorWS

395

465

 

LissamineRhodamine

572,577

591,592

 

LissamineRhodamineB

577

592

 

LIVE/DEADKitAnimalCells

Calcein/Ethidiumhomodimer

494

528

517

617

formoredetailsrefer

LOLO-1

566

580

 

LO-PRO-1

568

581

 

LuciferYellow

425,428

528,536,540

 

LysoTrackerBlue

373

422

 

LysoTrackerBlue-White

466

536

 

LysoTrackerGreen

504,534

511,551

 

LysoTrackerRed

490

516

 

LysoTrackerYellow

551

576

 

LysoSensorBlue

374

424

 

LysoSensorGreen

442

505

 

LysoSensorYellow/Blue

384

540

 

MagGreen

507

531

 

MagdalaRed(PhloxinB)

524

600

 

Mag-FuraRed

483/427

659/631

RatioDye,Mg2+

Mag-Fura-2

369/329

369/330

508

511/491

RatioDyeCa2+

RatioDyeMg2+

Mag-Fura-5

369/330

369/332

505/500

RatioDye,Ca2+

RatioDye,

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