精品荧光染料激发和发射光谱表.docx

1、精品荧光染料激发和发射光谱表荧光素名称激发波长(nm)发射波长(nm)备注1,5IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein(5-FAM)4925185-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH5-Carboxytetramethylrhodamine(5-TAMRA)5425685-FAM(5-Carboxyfluorescein)4925185

2、-HAT(HydroxyTryptamine)370-415520-5405-HydroxyTryptamine(HAT)370-415520-5405-ROX(carboxy-X-rhodamine)5785676045915-TAMRA(5-Carboxytetramethylrhodamine)5485425525686-Carboxyrhodamine6G5185436-CR6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-AminoactinomycinD(7-AAD)5466477-Hydroxy-4-methylcoumarin

3、360449,4559-Amino-6-chloro-2-methoxyacridine412,430471,474ABQ344445AcidFuchsin540630ACMA(9-Amino-6-chloro-2-methoxyacridine)412,430471,474AcridineOrange+DNA502526AcridineOrange+RNA460650AcridineOrange,bothDNA&RNA440-480520-650AcridineRed455-600560-680AcridineYellow470550Acriflavin436520AcriflavinFeu

4、lgenSITSA355-425460Aequorin(Photoprotein)466PhotoproteinAFPs-AutoFluorescentProtein-(QuantumBiotechnologies)seesgGFP,sgBFPAlexaFluor350346342442441AlexaFluor430431540AlexaFluor488495,492519,520AlexaFluor532531,532553,554AlexaFluor546556,557572,573AlexaFluor568577,578603AlexaFluor594590,594617,618Ale

5、xaFluor633632650AlexaFluor647647666AlexaFluor660668698AlexaFluor680679702AlizarinComplexon530-560,580580,624-645AlizarinRed530-560580Allophycocyanin(APC)630,645655,660AMC,AMCA-S345445AMCA(Aminomethylcoumarin)345347425444AMCA-X353442AminoactinomycinD555655Aminocoumarin346350442445Aminomethylcoumarin(

6、AMCA)345347425444AnilinBlue600Anthrocylstearate360-381446APC(Allophycocyanin)630,645655,660APC-Cy7625-650755APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+APTS424505AstrazonBrilliantRed4G500585AstrazonOrangeR470540AstrazonRed6B520595AstrazonYellow7GLL450480Atabrine436490ATTO-TAGCBQCA465560ATTO-TA

7、GFQ486591Auramine460550AurophosphineG450580Aurophosphine450-490515BAO9(Bisaminophenyloxadiazole)365395BCECF(highpH)492,503520,528BCECF(lowpH)482520BerberineSulphate430550BetaLactamase409447,520BFPblueshiftedGFP(Y66H)BlueFluorescentProtein381,382,383445,447,448blueshiftedGFP(Y66H)BlueFluorescentProte

8、inBFP/GFPFRETBimane398490Bisbenzamide360461Bisbenzimide(Hoechst)360461bis-BTC=RatioDye,Zn2+455/405529/505RatioDye,Zn2+BlancophorFFG390470BlancophorSV370435BOBO-1462481BOBO-3570602Bodipy492/515490515Bodipy493/503533549Bodipy500/510509515Bodipy505/515502510Bodipy530/550528547Bodipy542/563543563Bodipy5

9、58/568558569Bodipy564/570564570Bodipy576/589579590Bodipy581/591584592Bodipy630/650-X625642Bodipy650/665-X647665Bodipy665/676605676BodipyFl504,505511,513Bodipy FLATP505514BodipyFl-Ceramide505511BodipyR6GSE528547BodipyTMR542574BodipyTMR-Xconjugate544573BodipyTMR-X,SE544570BodipyTR589617BodipyTRATP5916

10、20BodipyTR-XSE588616BO-PRO-1462481BO-PRO-3544570BrilliantSulphoflavinFF430520BTC-RatioDyeCa2+464/401533/529RatioDyeCa2+BTC-5N-atioDye,Zn2+459/417517/532RatioDye,Zn2+Calcein494517CalceinBlue373440CalciumCrimson588,589611,615CalciumGreen501,506531CalciumGreen-1Ca2+Dye506531Ca2+DyeCalciumGreen-2Ca2+506

11、/503536Ca2+CalciumGreen-5NCa2+506532Ca2+CalciumGreen-C18Ca2+509530Ca2+CalciumOrange549575576CalcofluorWhite385,395,405437,440,445Carboxy-X-rhodamine(5-ROX)576601CascadeBlue377398399420423CascadeYellow399400550552Catecholamine410470CCF2(GeneBlazer)CFDA494520CFP-CyanFluorescentProtein430,433,436,474,4

12、75,476,CyanFluorescentProtein(453)(501)CFP/YFPFRETChlorophyll480650ChromomycinA436-460470ChromomycinA445575CL-NERF(RatioDye,pH)504/514540RatioDye,pHCMFDA494520CoelenterazineCa2+Dye,bioluminescence(429)465Ca2+Dye,bioluminescence,nativemoleculeCoelenterazinecp(Ca2+Dye,)(430)442Ca2+Dye,bioluminescenceC

13、oelenterazinef(437)473Ca2+Dye,bioluminescenceCoelenterazinefcp452Ca2+Dye,bioluminescenceCoelenterazineh(437)464Ca2+Dye,bioluminescenceCoelenterazinehcp(433)444Ca2+Dye,bioluminescenceCoelenterazineip441Ca2+Dye,bioluminescenceCoelenterazinen(431)467Ca2+Dye,bioluminescenceCoelenterazineO460575CoumarinP

14、halloidin387470C-phycocyanineCPMMethylcoumarin384469MethylcoumarinCTC400-450602CTCFormazanCy2489506Cy3.18554568Cy3.5581598Cy3514552554566570Cy5.18649666Cy5.5675695Cy5649666670Cy7710,743767,805CyanGFP433(453)475(501)cyclicAMPFluorosensor(FiCRhR)500517CyQuantCellProliferationAssay480520CellProliferati

15、onAssayDabcyl453Dansyl340578DansylAmine337517DansylCadaverine335518DansylChloride372518DansylDHPE336517Dansylfluoride356noneDAPI359461Dapoxyl403580Dapoxyl2374574Dapoxyl3373574DCFDA504529DCFH(DichlorodihydrofluoresceinDiacetate)505535DDAO463607DHR(Dihydorhodamine123)505534Di-4-ANEPPS496705Di-8-ANEPPS

16、(non-ratio)488498605713DiA(4-Di-16-ASP)456591DichlorodihydrofluoresceinDiacetate(DCFH)505535DiD-LipophilicTracer644665LipophilicTracerDiD(DiIC18(5)644665DIDS341415Dihydorhodamine123(DHR)505535DiI(DiIC18(3)549,551565Dinitrophenol349DiO(DiOC18(3)484,487501,502DiR748780LipophilicTracerDiR(DiIC18(7)7507

17、79DM-NERF(highpH)497/510540RatioDye,pHDNP349Dopamine340490-520DsRed558583RedfluorescentproteinDTAF494520DY-630-NHS621660HemicyanelabelforproteinsandDNADY-635-NHS634664HemicyanelabelforproteinsandDNAEBFP383447EnhancedBlueFluorescentProteinECFP436474EnhancedCyanFluorescentProteinEGFP488,498507,516Enha

18、ncedGreenFluorescentProteinELF97345530Eosin524545Erythrosin529,532554,555ErythrosinITC529555EthidiumBromide510,523595,605Ethidiumhomodimer-1(EthD-1)528617Euchrysin430540EukoLightEuropium(III)chlorideEYFP513,520527,532EnhancedYellowFluorescentProteinFastBlue360440FDA494520Feulgen(Pararosaniline)57062

19、5FIF(FormaldehydInducedFluorescence)405433FITC490,494520,525FITCAntibody493517FlazoOrange375-530612Fluo-3480506,506520,527Fluo-4494516Fluorescein(FITC)490,494520,525FluoresceinDiacetate494520Fluoro-Emerald495524Fluoro-Gold(Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM1-43479598FM4-4651564

20、0FuraRed(highpH)572657FuraRed/Fluo-3Fura-2,highcalcium335505ExcitationratiodyeFura-2,lowcalcium363512ExcitationratiodyeFura-2/BCECFGenacrylBrilliantRedB520590GenacrylBrilliantYellow10GF430485GenacrylPink3G470583GenacrylYellow5GF430475GeneBlazer(CCF2)GFP(S65T)498516GFPredshifted(rsGFP)498516GFPwildty

21、pe,non-UVexcitation(wtGFP)475509GFPwildtype,UVexcitation(wtGFP)395509GFPuv385508GloxalicAcid405460GranularBlue355425Haematoporphyrin530-560580Hoechst33258345487Hoechst33342347483Hoechst34580392440HPTS355465Hydroxycoumarin325-360386-455Hydroxystilbamidine(FluoroGold)361536Hydroxytryptamine400530Indo-

22、1,highcalcium330401EmissionratiodyeIndo-1,lowcalcium346475EmissionratiodyeIndodicarbocyanine(DiD)644665Indotricarbocyanine(DiR)748780IntrawhiteCf360430JC-1514529JO-JO-1530545JO-PRO-1532544LaserPro795812Laurodan355460LDS751(DNA)543712LDS751(RNA)590607LeucophorPAF370430LeucophorSF380465LeucophorWS3954

23、65LissamineRhodamine572,577591,592LissamineRhodamineB577592LIVE/DEADKitAnimalCellsCalcein/Ethidiumhomodimer494528517617formoredetailsreferLOLO-1566580LO-PRO-1568581LuciferYellow425,428528,536,540LysoTrackerBlue373422LysoTrackerBlue-White466536LysoTrackerGreen504,534511,551LysoTrackerRed490516LysoTrackerYellow551576LysoSensorBlue374424LysoSensorGreen442505LysoSensorYellow/Blue384540MagGreen507531MagdalaRed(PhloxinB)524600Mag-FuraRed483/427659/631RatioDye,Mg2+Mag-Fura-2369/329369/330508511/491RatioDyeCa2+RatioDyeMg2+Mag-Fura-5369/330369/332505/500RatioDye,Ca2+RatioDye,