生化英文教学大纲文档格式.docx
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I.Grasptheconceptofbiochemistry
II.Acquaintwiththemaincontentsofbiochemistry
III.Knowtherelationbetweenthebiochemistryandmedicalscience
Chapter2
Structureandfunctionofprotein
I.Importanceofproteininlifeprocess
II.Moleculeconstituteofprotein
i.Chemicalelementoftheprotein
ii.Basicstructureunitofprotein—aminoacids
iii.Peptide
iv.Proteinclassification
III.Molecularstructureofprotein
i.Primarystructure
ii.Secondarystructure
iii.Tertiarystructure
iv.Quaternarystructure
IV.Relationshipbetweenthestructureandfunctionofprotein
i.Relationshipofprimarystructureandfunctionofprotein
ii.Relationshipofspatialstructureandfunctionofprotein
V.Physicsandchemistryproperty,separationandpurificationofprotein
i.Physicsandchemistryproperty
ii.Separationandpurification
iii.Aminoacidssequenceanalysisofpolypeptidechain
iv.Spatialstructuremeasurementofprotein
I.Knowthecharacteristicsofchemicalelementofprotein,graspthebasicstructureofaminoacidsandacquaintwiththeclassificationandthephysicsandchemistrypropertyofaminoacids.
II.Graspthebasicconceptofthepeptidebondandpeptidechainandtheprimarystructurecharacteristicsofinsulin.Knowafewimportantbioactivepeptide.
III.Knowtheprinciplesofproteinclassification.
IV.Graspthefamiliartypeofsecondarystructure:
alpha-helix,beta-pleatedsheet,beta-turnandrandomcoil.Acquaintwiththeconceptofthepeptideunit,motif,andchaperon.
V.Grasptheconceptoftertiarystructureanddomain.Analyzethetertiarystructurecharacteristicsofribonuclease.
VI.Grasptheconceptofquaternarystructureandanalyzethequaternarystructurecharacteristicsofhemoglobin(Hb).
VII.Grasptherelationshipbetweenprimarystructureandfunctionofprotein.Acquaintwiththeconceptof"
moleculardisease"
.
VIII.Grasptherelationshipbetweenspatialstructureandfunctionofproteinandacquaintwithrelationshipbetweentheconformationvarietyandthefunctionofbindingtooxygenofhemoglobin.
IX.Grasptheampholyteproperty,highmolecularweightanddenaturationofprotein.Acquaintwithprecipitationandofproteinultravioletabsorption.
X.Grasptheelectrophoresisandgelfiltrationofprotein.Knowthesaltprecipitation,isoelectricpoint,ultrafiltrationandultracentrifugation.
XI.Knowtheprinciplesofprimarystructuremeasurementofprotein.
XII.Knowtheprincipleandmeaningofthespatialstructureestimationofprotein.
Chapter3
Structureandfunctionofnucleicacid
I.Chemicalconstituteofnucleicacid
i.Basecomponentofnucleotide
ii.Pentoseandnucleoside
iii.Structureandnamingofnucleotide
II.Primarystructureofnucleicacid
III.Spatialstructureandfunctionofdeoxyribonucleicacid(DNA)
i.SecondarystructureofDNA——doublehelixstructuremodel
ii.SuperhelixofDNA
iii.FunctionofDNA
IV.Spatialstructureandfunctionofribonucleicacid(RNA)
i.Messengerribonucleicacid(mRNA)
ii.Transferribonucleicacid(tRNA)
iii.Ribosomalribonucleicacid(rRNA)
iv.OthersmallribonucleicacidandRNomics
v.Ribozyme
V.Physicsandchemistrypropertyofnucleicacidanditsapplication
i.Generalphysicsandchemistryproperty
ii.DNAdenaturation
iii.DNArenaturation(annealing)andhybridization
VI.Nuclease
I.Graspthestructure,symbolandpropertyoffamiliarnucleotides.GraspthemolecularconstituteofDNAandRNA.Grasptheconnectionpatternandthebonddirectivityofnucleotide.Grasptheprimarystructureofnucleicacidanditsrepresentationalmethod.
II.GraspthesecondarystructureofDNAandtRNA——doublehelixstructuremodelandcloverleafpattern.
III.Graspthedenaturation,renaturation,unwindingcurveandmeltingtemperatureofDNA.
IV.Knowthefollowingconcepts:
annealing,hybridization,ribozymeandnuclease.
Chapter4Enzyme
I.Molecularstructureandfunctionofenzyme
i.Molecularconstitute:
simpleenzyme,conjugatedenzyme,coenzymeandcofactor
ii.Activecenterofenzyme
II.Characteristicsandmechanismofenzyme-catalyzedreaction
i.Characteristics:
highefficiency,highspecificityandadjustability.
iii.Mechanism
III.Kineticsofenzyme-catalyzedreaction
i.Effectsofsubstrateconcentration
ii.Effectsofenzymeconcentration
iii.Effectsoftemperature
iv.EffectsofpHvalue
v.Effectsofinhibitor
vi.Effectsofactivator
vii.Activitymeasurementandactiveunitofenzyme
IV.Regulationofenzyme
i.Activationofenzymeandzymogen.Allostericenzyme.Covalentmodificationofenzyme
ii.Regulationofenzymequantity
iii.Isoenzyme
V.Classificationandnamingofenzyme
VI.Relationshipbetweenenzymeandmedicalscience
i.Enzymeanddiseaseoccurrence
ii.Enzymeanddiseasediagnosis
iii.Enzymeanddiseasetherapy
iv.Other
I.Grasptheopinionsthatenzymeisessentiallyaprotein,sogenerallyallnotheat-proof,andthefactorsleadingtoproteindenaturationallhaveinfluencetotheactivityofenzymeeasily.
II.Acquaintwithmolecularconstituteofenzymeandgraspenzymeactivitycenter.
III.Acquaintwiththecharacteristicsofenzyme-catalyzedreactionandKnowthemechanismofenzyme-catalyzedreaction.
V.Grasptheeffectsofsubstrateconcentrationonenzyme-catalyzedreaction:
Michaelisequation,measurementandmeaningofMichaelisconstant(Km)andmaximumvelocity(Vmax).
Grasptheeffectsofinhibitoronenzyme-catalyzedreaction:
kineticscharacteristicsandphysiologicalsignificance
Acquaintwiththemeasurementofenzymeactivity,conceptofenzymeactivityunitandtheeffectsofactivatoronenzyme-catalyzedreaction
VI.Graspthephysiologicalsignificanceandmechanismofzymogenactivation.
Grasptheconceptandregulativefunctionofallostericenzyme
Grasptheconceptandphysiologicalsignificanceofsynergisticeffectandenzymecovalentmodification
Grasptheconcept,molecularcharacteristicsandregulativesignificanceofisoenzyme
VII.Knowtheclassificationandnamingprinciplesofenzyme
VIII.Acquaintwithapplicationofenzymeindiseaseoccurrence,diagnosisandtherapy
Chapter5
Carbohydratemetabolism
I.Physiologicalfunction,digestionandabsorptionofcarbohydrate
II.Anaerobicdecompositionofcarbohydrate:
reactionprocess,regulationofglysolysisandphysiologicalsignificance
III.Aerobicoxidationofcarbohydrate:
reactionprocess,ATPproduction,regulationofaerobicoxidationandPastuereffect
IV.Pentosephosphatepathway:
reactionprocess,regulationandphysiologicalsignificance
V.Synthesisanddecompositionofglycogen
i.Synthesisanddecompositionofliverglycogen
ii.Regulationofliverglycogensynthesisanddecomposition
iii.Synthesisanddecompositionofmuscleglycogenandglycogenstoragedisease
VI.Gluconeogenesis:
process,regulation,physiologicalsignificanceandCoricycle
VII.Bloodsugaranditsregulation
i.Sourceandoutletofbloodsugar
ii.Hormoneregulatingtheconcentrationofbloodsugar
iii.Abnormalityofbloodsugarlevel
I.Knowthephysiologicalfunctionofcarbohydrateanditsdigestionandabsorptioninbody
II.Graspthereactionprocess,rate-limitingenzyme,ATPproduction,regulationandphysiologicalsignificanceofglycolysispathway
III.Graspthebasicreactionsintheprocessofpyruvateoxidativedecarboxylationandtricarboxylicacidcycle.Grasptherate-limitingenzyme,ATPproduction,regulationandphysiologicalsignificanceofaerobicoxidationpathway.AcquaintwithPastuereffect
IV.Knowthereactionprocessandregulationofpentosephosphatepathway,andgraspthephysiologicalsignificanceofpentosephosphatepathway
V.Acquaintwiththebasicreactionintheprocessofliverglycogensynthesisanddecomposition.Grasptheregulationofliverglycogensynthesisanddecomposition.Knowtheregulationofmuscleglycogensynthesisanddecomposition.Knowglycogenstoragedisease
VI.Grasptheconceptofgluconeogenesisandacquaintwiththebasicreaction,regulationandphysiologicalsignificanceofgluconeogenesispathway.AcquaintwithCoricycleanditsphysiologicalsignificance.
VII.Acquaintwiththenormalconcentrationofbloodsugarandhormoneregulatingtheconcentrationofblo