王镜岩版《生物化学》总复习习题.docx

王镜岩版《生物化学》总复习习题.docx

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王镜岩版《生物化学》总复习习题

王镜岩版《生物化学》总复习习题（TherevisionofbiochemistryinWang'sEdition）

Biochemicalchapters

Thirdchapteraminoacids

Questionanswering:

1.whatarethecharacteristicsofacidhydrolysis,alkalinehydrolysisandenzymatichydrolysisofproteins?

2.writethestructureof20basicaminoacids,threeacronymsandacronyms.

3.whatarethecharacteristicsofglycine,histidineandproline?

4.whatistheisoelectricpointofanaminoacid?

Writedownthestructureanddissociationprocessoftheaminoacids,andcalculatetheisoelectricpoints:

valine,glutamicacidandarginine.

5.inthesynthesisofpeptides,theaminoacidsofaminoacidsneedtobeprotected.Whatreactionscanprotecttheaminogroup?

Whatarethe6.SangerreagentsandEdmanreagents?

Howdoesitreactwithaminoacids?

What'sthepointofthisreaction?

7.,trytowriteoutthereactionbetweencysteineandethyleneimine.What'sthesignificanceofthisreaction?

8.writethereactionbetweentheoxidantandthereducingagentthatopensthecystinetwosulfurbond.

9.whatarethereasonsfortheUVabsorptionofproteins,andwhatisthemaximumabsorptionpeak?

10.whatisthelawofdistributionandthecoefficientofdistribution?

Whatistheprincipleofpartitionchromatography?

11.whatisHPLC?

12.textbookP156,15.

Thefourth,fifthchapteristhecovalentstructureandthree-dimensionalstructureofproteins

I.NOUNinterpretation:

Thepureprotein（forexample）,conjugatedprotein（forexample）,thecofactor,ligand,oneortwo,threeorfourlevelstructure,proteinsupersecondarystructure,domain,

Peptideplanes（amideplanes）,glutathione（structural）,diagonalelectrophoresis,completehydrolysis,partialhydrolysis,homologousproteins,invariantresidues,variableresidues,

Alphahelixbetafolding,membraneintrinsicproteins,lipidanchoredmembraneproteins,proteindenaturationandrenaturation,monomers,polymers,andheteroproteins

Two.Answerthequestion:

1.attemptstoillustratethediversityofproteinfunction?

2.ofthoseexperimentscanexplainwithproteinpeptidebond?

Thenatureofthepeptidebond3.

4.testthestrategyofprimarystructuredeterminationofprotein.

5.howtodetermineN-terminalaminoacids?

6.illustratesthespecificactionoftrypsin,chymotrypsin,thermophilicproteaseandpepsin.

7.booksp194-secondquestions

8.whatarethemethodsforstudyingproteinconformation?

9.whatarethethreemicrostructuralforcesthatstabilizeproteins?

10.whatarethefactorsthataffecttheformationofalphahelix?

11.whatarethecharacteristicsoftheaminoacidcompositionofcollagen?

12.whatarethesymptomsafterproteindenaturation?

13.illustratesthattheprimarystructureofproteinsdeterminesthethreestructure.

Thesixthchapteristherelationshipbetweenproteinstructureandfunction

I.NOUNinterpretation:

Globin,heme,methemoglobin,ferrousmyoglobin,methemoglobin

Two.Answerthequestion:

1.whatisthedifferenceintheoxygenationcurvebetweenmyoglobinandhemoglobin?

Trytoexplaintherelationshipbetweenproteinstructureandfunction.

Whateffectdo2.,H+,CO2,andBPGhaveonhemoglobinbindingoxygen?

3..Themolecularmechanismofsicklecellanemia.

Theseventhchapteristheseparation,purificationandcharacterizationofproteins

I.nouninterpretation

Plasmapoint,sedimentationcoefficient,saltingout,saltdissolution,densitygradientcentrifugation,dialysis,ultrafiltration,gelfiltration,SDA-PAGEelectrophoresis,isoelectricfocusing,

affinitychromatography

Two.Answerthequestion:

1.whatarethemethodsforseparatingproteinsaccordingtotheirsize,solubility,andcharge?

2.nameseveralmethodsofdeterminingproteincomponents.

Three

Whatarethemethodsofproteincontentmeasurement?

4.books,p317,first,5,7,8

Theeighthchapterontheenzyme

Textbookp348-349all

Theninthchapteristhekineticsofenzymaticreaction

I.NOUNinterpretation:

Thetheoryofintermediatecomplexes,inactivation,inhibition,temperaturecoefficient,activator,MichaelisMentenequation,inhibitionpercentage,reversibleinhibition,irreversibleinhibition

Example）competitiveinhibition（forexample）,noncompetitiveinhibition

Two.Answerthequestion:

Whatisthesignificanceofthe1.Michaelisconstant?

2.whatarethecharacteristicsofcompetitiveinhibitionandnoncompetitiveinhibition?

3.trytodescribetheprinciplethatsulfadrugscaninhibitthegrowthofbacteria.

4.whatarethecharacteristicsoftheinfluenceoftemperatureandpHonenzymaticreaction?

Thetenthchapteristhemechanismofenzymeactionandtheregulationofenzymes

I.NOUNinterpretation:

Theactivesiteoftheenzyme,zymogen,zymogenactivation,covalentmodification,isozyme（forexample）

Two.Answerthequestion:

1.totesttheeffectofDFPonchymotrypsinmodificatio