羊胚胎移植项目Embryo Transfer ProgramWord格式文档下载.docx

羊胚胎移植项目Embryo Transfer ProgramWord格式文档下载.docx

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Manyoftherecipientdoeswillcomefromourexistingherd.Allthedoeswhokiddedinthefallof2003.exceptVenus,willbeusedbecauseweknowtheircapabilities,theirhealthhistory,andtheirmotheringabilities.Allkidsfromthefall2003crophavegainedmorethan½

poundadaywithnosupplementsotherthanthemothersarebeingfedtwiceadayinsteadofonceaday

Twoofourkikos,HollyandEileen

But,weneedaminimumof10to12recipientdoes;

so,weattendedthemonthlyauctionatElginCrossroadson12Dec03withtheintentionofbuyingsomerecipientsifthepricewasright.

Thepriceswereextremelylow.Wedecidedtobuydoeswithkidsbytheirside.Thatwaywewouldbeabletodeterminetheirmilkcapacityanddeterminetheirmotheringskills.

Note:

anytimeyoupurchasefromasalesbarn,youneverknowwhatyouaregoingtobebringinghome–inthewayofqualityordisease.Itis,therefore,extremelyimportanttohavean“isolationarea”wherethegoatscanbekeptandmonitoredbeforejoiningtherestofyourherd.AllourpurchasesfromsalebarnsaretestedforCL,CAE,andJohnesasaprecaution.Ourintentisnottoallowthesereceiptstojointheherdatall.

Assoonaswebroughtthegoatshome,wewormedthemthreedaysinarowwithValbazen.Oneweekafterthefinalwormingwiththewhitewormer,wewormedwithanIvomectinproduct.Oneofthethreegoatsweboughthadaverymildcaseofdiarrhea,sowechoseanaggressivelytreatmentofBiosolandKaopectin.

Wealsowenttoourvet’sandpickedupthreesyringesof3cclutylasejustincasethesedoeswerepregnant（althoughwewerecertain,basedontheageofthekidsonthem,theywouldnotbebredback）.

Attheauction,theysoldthemalekidsoffoneofthedoes.Basedonthesizeofthekids,weguessedtheywere2monthsold.Twodaysafterwegavethisdoeherlutylaseshot,sheaborted3fetuses–twogirlsandaboy–whowerewell-formedandclearlyinthelasttrimesterofthepregnancy.Theclearindicationisthekidsbyhersideattheauctionwerenothers.Theothertwodoeshadnonoticeablereactiontothelutylase.

OneofthethreedoeswepurchasedasarecipientisafullbloodBoerwithaweek-olddoekid.She’slongandextremelythinbut,withthepropernutrition,shouldbeeasilycapableofcarryingtwoembryos.TheothertwodoesareKikos–one（theonewhoabortedthe3fetuses）appearstobeapproximately4yearsoldandisextremelymalnourished;

again,properfeedingandanoccasionalfortifiedVitaminBcomplexshotshouldimprovehercondition.BoththeKikoshavealargecapacityandcaneasilycarrytwo（ormore）embryos.

Withtheactualflushdatesetandonthehorizon,wespentseveraldaysmakingourfinalpreparations…gettingthedoesready.

InJanuarywemadethedecisiontodefinitelyuseKattieasourdonordoeinsteadofVenus.Venusmaybeusedinournextflushifthisoneissuccessful.WeremovedKattie'

smalekidinmid-JanuarybutleftSamanthaforKattietowean.Inretrospectthismayhavebeenamistake;

weprobablyshouldhaveweanedSamanthawhenweremovedThunderbolt.

OnJanuary24thwepickedupthelasttwodoesweneedforreceips.DamonDeese,themanageroftheLawrenceCountyCo-Op,isloaningustwoofhisdoes.Inreturnwewillallowhimtobreedhisremainingdoestooneofourbucksandreturnthetwodoesbred.Sincehedoesnothaveabuck,wedeterminedwewouldgivehimabuckkidfromtheETbreeding.Damon,inloaningusthedoes,isjustbeingagoodneighbor.

ArrangementswereconfirmedwithJeffLathamoftheLazyLFarminLivingston,AL.WeweretoldCIDRsweretogoinonMarch1st;

thebreeddatewouldbeMarch18th;

andtheflushwouldtakeplaceonMarch25th.SincethebreeddateconflictedwiththegoatshowinMontgomery,AL,wecancelledourplanstoattendtheshow.

Jeffrecommendedwestarttoincreasetheamountoffeedforthedonorandreceipsamonthtosixweeksbeforetheflush.Beforewecouldincreasethefeed,wefeltweneededtoweanthebabiesofKattie,ourdonor,andsevenoftheelevenreceipssothesedoescoulddryup.Samanthawasalmost4monthsold;

timetoweanherregardlessofhowmuchKattieprotested.Dot,Matilda,Xenia,Koko,andLouisehadallkiddedinNovember,sotheirkidswereatleast10weeksoldwhenweweanedthem.Faith'

sdaughter,Gidget,wasonly8weeksoldaswasKoko'

slittleboy;

butbothkidswereeatingwell.Sothekidswentinonepaddockandthemothersinanother,andwehadalotofextremelyunhappygoatsforabouttwoweeks.（Themothersmoreunhappythanthebabiesbecausenotonlyweretheyuncomfortablewithfulludders,theyalsowereconfidentwewerestarvingthembyfeedingonlyhayandwater!

）

WegotacallfromJeffearlythemorningofFebruary24th.TheflushdatehadmoveduptoMarch20th,sothebreeddatewasadvancedtoMarch13thandCIDRshadtogoinonFebruary26th.Mildpanic!

SevenofthereceipsandKattiewerenotfullydriedoff,andwehadtoimmediatelystartincreasingtheirfeedto4poundsaday.KencalledDr.Galbraith'

s,andhehadmedicationtodryupthedoes–1ccofForsomidefor3days.The8gottheirfirstshotsonthe24th.

OnFebruary25th,the8doesgottheirsecondshottodryoff;

andall12doesweregiven2ccvitaminAD&

Eplus2ccBoSe.All12werealsogivenweight-appropriatedosesofIvomecPlus,andtheyallgotpedicures.

Alsoonthe25th,Jeffshippedus14CIDRsandFolltropin®

–Valongwithwritteninstructionsforustofollow.

OntheafternoonofFebruary26th,UPSdeliveredoursupplies.The"

tails"

ofalltheCIDRswerepaintedwithbrightredfingernailpolish,andtheball-liketipswerecutoff.（ThetipswerecutofftopreventotherdoesfrompullingouttheCIDRs.Thewhitestringswerepaintedredsowecouldseethemandinsuretheyremainwheretheybelong.）

CIDR

Thattaskaccomplished,offtothebarntoinsertCIDRs.

CIDRsareintravaginalprogesteronereleasedevicesforcontrolledbreedingofgoatsandsheep.EachCIDRcontains.3gramsofprogesterone.Besidesbeingusedinembryotransferprograms,theyarefrequentlyusedin“outofseason”breedingofsomebreedsbecausetheycontroltheoestrouscycle.Whenusedwithgoatstheinstructionssaytoinsertthedevicefor17to19days.

Thereare7stepsintheinsertiondirections.Besuretowearrubbergloves.

1.Washtheapplicatorinanantisepticsolution.Dipthefrontoftheapplicatorintoanobstetricallubricant.

2.PlacetheCIDRintotheapplicatorsothewingsarefoldedandonlythetipsofthewingsprotrudefromthetopoftheapplicator.

3.PutsomeoftheKY-Jellyontheendoftheapplicator.

4.Liftthetailoftheanimalandwipecleanthelipsofthevulvawithadisposabletissue.

5.Withtheanimal’staillifted,inserttheloadedapplicator,slopingitslightlyupwards,throughthevulvaandintotheforwardportionofthevagina.

6.Graspthefingerholdsoftheapplicatorandfirmlypullthebarreloftheapplicatortowardthehandletoremove.

7.Ifthedeviceiscorrectlyinplace,thewingswillopenintheanteriorofthevaginaandthetailoftheCIDRisvisiblyprotrudingfromthevulva.

TheCIDRsweusedwereEAZI-BREED™byPharmacia&

UpjohnoutofMt.Wellington,Auckland.

TheCIDRsactuallywentinaloteasierthanwewereanticipating.Bythelastonewehaditdowntoashortroutine.The8doesalsogottheirfinalshottodryoff.

Withthegoats,donorandreceips,onceagainbeingfedfullrations（2poundsoffeedeach–twiceaday）andthepreliminarypreparationscompleted,we'

rereadyforthenextstep.

Onthe12thdayaftertheCIDRswereinserted,itwastimetostartthehormoneshotsforKattie,ourdonordoe.ThefirsttwoshotsofFolltropin®

-Vweretobeintheneck.Sincewehadnevergivenshotsinthisareaandsincewewantedthisdrugtobecorrectlyadministrated,KentookKattietothevet'

s.（AndsincealldoesneededhealthcertificatespriortotraveltoAlabama,thiswasaccomplishedatthesametime.）

Inaddition,Corinahadbeen"

off"

sincethethirddayaftertheCIDRswereinserted.Shewasnoteating;

shehadanormaltemperature;

butshewaswalkingaroundwithherheaddownorjustlayingaroundthebarnwithherheadagainstthewall.Fecalswerecheckedforparasites,andbloodtestsconducted.Ourvetcouldfindnothingwrongwithher,andthreedayslater,whentheresultsofthebloodtestswerereceived,hernamewasaddedtothehealthcertificate.

Falltropin-VBottles

Thefirstshot,intheafternoon,was1.5ccofFolltropin®

-V,amedicinethatcameintwobottlesthathadtobemixed.（Onebottlewasthedrugitself;

thesecondbottlewasasterileDiluent.）ThenextmorningKattiegot1.5cc.Thatafternoonshegotanother1.25cc.Onday14shegot1.25ccinthemorningandanadditional1ccintheafternoon.Thefollowingdayshegot1ccinthemorningand.75ccintheafternoon.Finally,themorningfollowingtheremovalofherCIDRshereceivedherlastshotaFolltropin®

-V,.5cc.Thislastshotwasonbreedingday.

Onday15theCIDRswereremovedfromalltherecipients.Kattie'