果胶酶处理果胶制备方法及其应用.docx

果胶酶处理果胶制备方法及其应用.docx

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果胶酶处理果胶制备方法及其应用

Pecticenzymetreated pectin, methodofproducingthesameandapplicationthereof 

Abstract

Apecticenzymetreatedpectin（PET-pectin）andamethodofproducingthesamearedisclosed.Citruspectinsolutionissubjectedtoanenzymatichydrolysisbyusingamixedpectinasesolutionunderanacidiccondition,soastoobtainPET-pectinthatiscompletelyhydrolyzed.ThePET-pectinhasseveralbiologicalfunctions,includingstabilityofemulsification,anti-oxidationandinhibitionofcancercellgrowth,therebybeingappliedonfoodemulsionstabilizers,foodantioxidantsandcompositionsforinhibitingcancercellgrowthandsoon.

Inventors:

Wu;Ming-Chang （Pingtung, TW）,Wang;Yuh-Tai （Taipei, TW）,Huang;Ping-Hsiu （Taichung, TW）

Applicant:

Name

City

State

Country

Type

Wu;Ming-Chang 

Wang;Yuh-Tai 

Huang;Ping-Hsiu

Pingtung 

Taipei 

Taichung

N/A 

N/A 

N/A

TW 

TW 

TW

Assignee:

NATIONALPINGTUNGUNIVERSITYOFSCIENCEANDTECHNOLOGY （Neipu, TW） 

FamilyID:

48281204

Appl.No.:

13/490,065

Filed:

June6,2012

PriorPublicationData

DocumentIdentifier

PublicationDate

US20130123211A1

May16,2013

ForeignApplicationPriorityData

Nov10,2011[TW]

100141068A

CurrentU.S.Class:

1/1

CurrentCPCClass:

A23L1/0524 （20130101）;C12P19/04 （20130101）;A23D7/0053 （20130101）;C11B5/0021 （20130101）;C12Y402/0201 （20130101）;C12Y301/01011 （20130101）;A61K31/732 （20130101）;C12Y302/01015 （20130101）;Y02P20/52 （20151101）

CurrentInternationalClass:

A61K31/70 （20060101）;A23D7/005 （20060101）;C12P19/04 （20060101）;A23L1/0524 （20060101）;C11B5/00 （20060101）;A61K31/732 （20060101）

ReferencesCited [ReferencedBy]

U.S.PatentDocuments

7491708

February2009

Platt

ForeignPatentDocuments

200624043

Jul2006

JP

200624043

Jul2006

TW

OtherReferences

Kissetal.,"KineticStudyonHydrolysisofVariousPectinsbyAspergillusNigerPolygalacturonase",HungarianJournalofIndustrialChemistryVESPREM,vol.36（1-2）,pp.55-58（2008）.citedbyexaminer.

Huangetal,"AntioxidantActivityandEmulsion-StabilizingEffectofPectinEnzymeTreatedPectininSoyIsolate-StabilizedOil/WaterEmulsion",JournalofAgriculturalandFoodChemistry,vol.59（2011）,pp.9623-9628）.citedbyexaminer.

Crinnion,Walter,IsModifiedCitrusPectinanEffectiveMobilizerofHeavyMetalsinHumans?

AlternativeMedicineReviewvol.13,No.42008.citedbyapplicant.

Eliaz,etal.,TheEffectofModifiedCitrusPectinonUrinaryExcretionofToxicElements,PublishedonlineJul.11,2006inWileyInterScience（）DOI:

10.1002/ptr.1953.citedbyapplicant.

Funami,etal.,Effectsoftheproteinaceousmoietyontheemulsifyingpropertiesofsugarbeetpectin,FoodHydrocolloids21（2007）1319-1329.citedbyapplicant.

Guess,etal.,Modifiedcitruspectin（MCP）increasestheprostate-specificantigendoublingtimeinmenwithprostatecancer:

aphaseIIpilotstudy,ProstateCancerandProstaticDiseases（2003）6,301-304,2003NaturePublishingGroup.citedbyapplicant.

Hayashi,etal.,EffectsofDailyOralAdministrationofQuercetinChalconeandModifiedCitrusPectinonImplantedColon-25TumorGrowthinBalb-cMice,AlternativeMedicineReview,vol.5,No.6,2000.citedbyapplicant.

Huang,etal.,Theuptakeofoligogalacturonideanditseffectongrowthinhibition,lactatedehydrogenaseactivityandgalactin-3releaseofhumancancercells,FoodChemistry（2012）,doi:

10.1016/j.foodchem.2011.12.037.citedbyapplicant.

Jackson,etal.,Pectininducesapoptosisinhumanprostatecancercells:

correlationofapoptoticfunctionwithpectinstructure,Glycobiologyvol.17No.8pp.805-819,2007.citedbyapplicant.

Kidd,Parris,ANewApproachtoMetastaticCancerPrevention:

ModifiedCitrusPectin（MCP）,AUniquePectinthatBlocksCellSurfaceLectins,AlternativeMedicineReview,vol.1,No.1,1996.citedbyapplicant.

Liu,etal.,Inhibitoryeffectofmodifiedcitruspectinonlivermetastasesinamousecoloncancermodel,WorldJGastroenterol,Dec.28,2008;14（48）:

7386-7391,WorldJournalofGastroenterologyISSN1007-9327.citedbyapplicant.

Lutz,etal.,Ontheconfocalimagesandtherheologyofwheyproteinisolatedandmodifiedpectinsassociatedcomplex,ColloidsandSurfacesB:

Biointerfaces69（2009）43-50.citedbyapplicant.

Nangia-Makker,etal.,InhibitionofHumanCancerCellGrowthandMetastasisinNudeMicebyOralIntakeofModifiedCitrusPectin,JournaloftheNationalCancerInstitute,vol.94,No.24,Dec.18,2002.citedbyapplicant.

Rediguieri,etal.,ThermodynamicIncompatibilityandComplexFormationinPectin/CaseinateMixtures,Biomacromolecules2007,8,3345-3354.citedbyapplicant.

Veldman,etal.,Dietarypectininfluencesfibrinnetworkstructureinhypercholesterolaemicsubjects,ThrombosisResearch,vol.86,No.3,pp.183-196,1997.citedbyapplicant.

PrimaryExaminer:

 Peselev;Elli 

Attorney,AgentorFirm:

 Glenn;MichaelA.PerkinsCoieLLP 

Claims

Whatisclaimedis:

1.Amethodofproducingpecticenzymetreatedpectin（PET-pectin）,comprising:

subjectingCitruspectinsolutiontoanenzymatichydrolysisbyusingamixedpectinasesolutionunderanacidicconditionofpH4.0andatemperatureof45degreeCelsius（.degree.C.）for12hourto48hours,soastoobtainpectinhydrolysate,whereintheCitruspectinsolutionhasapectinconcentrationof1percentbyvolumeto3percentbyvolume,themixedpectinasesolutioncomprisespectinmethylesterase（PME）,polygalacturonase（PG）andpectinlyase（PL）,andavolumeratioofthemixedpectinasesolutiontotheCitruspectinsolutionis1:

1000;andsubjectingthepectinhydrolysatetoathermaltreatmentunderatemperatureof100.degree.C.for10minutes,soastoterminatetheenzymatichydrolysisandobtainPET-pectinthatiscompletelyhydrolyzed,whereinthePET-pectinhasanaveragedmolecularweightoflessthanorequalto1kilodalton（kDa）,anaverageddegreeofesterification（DE）of11.6%andanaverageddiameterof2000nanometers（nm）. 

2.ThemethodofproducingPET-pectinofclaim1,whereintheCitruspectinsolutionhasthepectinconcentrationof1percentbyvolume. 

3.ThemethodofproducingPET-pectinofclaim1,whereinthePET-pectininhibitgrowthofcancercells3percentsto60percents. 

4.ThemethodofproducingPET-pectinofclaim3,whereinthecancercellsareselectedfromthegroupconsistingoflivercancercells,lungcancercellsorcolorectalcancercells. 

5.ThemethodofproducingPET-pectinofclaim1,whereinthePET-pectinhas1.1to2.9foldsofanemulsificationactivityand1.2to2.0foldsofanemulsificationstabilitybasedontheemulsificationactivityandtheemulsificationstabilityofoil-in-wateremulsionstabilizedbyasoybeanisolatedproteincalculatedasonefold. 

6.ThemethodofproducingPET-pectinofclaim1,whereintroloxequivalentantioxidantcapacity（TEAC）ofthePET-pectinperonegramisequivalentto2.2mMto4.3mMtrolox.

Description

RELATEDAPPLICATIONS 

ThisapplicationclaimsprioritytoTaiwanApplicationSerialNumber100141068,filedonNov.10,2011,whichishereinincorporatedbyreference. 

BACKGROUND 

1.FieldofInvention 

Thepresentinventionrelatestoapecticenzymetreatedpectin（PET-pectin）andamethodofproducingthesame.Moreparticularly,thepresentinventionrelatestoaPET-pectinwithbiologicalfunctions,includingstabilityofemulsification,anti-oxidationandinhibitionofcancercellgrowth,amethodofproducingthesameandapplicationthereof. 

2.DescriptionofRelatedArt 

Afterthestormsofillegaladditionofplasticizersinfoods,thesafetyoffoodadditivesareconcernedmore.AccordingtoArticle3ofActofGoverningFoodSanitationinTaiwan,theterm"foodadditives"shallmeanmaterialsthatareaddedtoorbroughtintocontactwithfoodsinthecourseofmanufacturing,processing,preparation,packaging,transportationandstorageoffoodsforthepurposeofcoloring,seasoning,preserving,bleaching,emulsifying,flavoring,stabilizingquality,enhancingfermentation,increasingviscosity,enrichingnutritionalvalue,preventingoxidationorotherwise.Currently,theallowedfoodadditivesaregenerallydividedintotwocategories:

naturalandchemicallysynthetic.Moreover,thefoodadditiveisnotnormallyconsumedasafoodbyitself. 

Pectinisoneofthenaturalfoodadditiveswidelyusedinfoodadditives.Pectinisoneofpolysaccharidescommonlyfoundinthemiddlelamellaofthecellwallsinhigherplantsforboundingcellstogether,anditissecondonlytocelluloseinannualproduction.PectinisapolymerconsistingmainlyofesterifiedD-galacturonicacidresidesinanalpha（.alpha.）-1.fwdarw.4glycosidelinkage.Furthermore,pectinisalegalandnaturalfoodadditiveforthepurposeofincreasingviscosityandadjustingthesweetnesswithoutlegalrestrictionsrelatedtoitsusage. 

Typically,theconventionalmodifiedpectinismadethroughchemicalreactionsbyaddingchemicalreagentssuchasacidsoralkalis.However,thechemicalreagentsmayremaininthemodifiedpectin.Moreover,thedrainageoftherecoveredchemicalreagentsmaypollutetheenvironment. 

Thereis,however,acontinuousneedfornaturallyandnon-chemicallymodifiedpectinandamethodofproducingthesame,forovercomingallissuesofchemicallymodifiedpectinandexploringmoreapplicationsofthemodifiedpectin. 

SUMMARY 

Amethodofproducingpecticenzymetreatedpectin（PET-pectin）isprovided.ThemethodcomprisestosubjectCit