第七章英文科技论文写作图文完整版.docx
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第七章英文科技论文写作图文完整版
•DistinctPyk2stainingisobservedinmeiosisIcleavagefurrowsinourstudy.MicroinjectingPyk2antibodyintotheoocyteatGVstageresultsinaninhibitionofthefirstpolarbodyemissioninourexperiment.ThismayindicatethatPyk2functionsasaregulatoroftheproteinsparticipatingincontractileringformationsuchasF-actinand/ormyosinorapartofthecontractileapparatusduringthefirstpolarbodyemission.However,thefirstpolarbodyemissionislowerintheIgGinjectiongroup(controlgroup,56.1%comparedwiththenormalculturedoocytes(about75%andthepatternofF-actinstainingisquitedifferentbetweenthemicroinjectedoocytesandthenormalcultureoocytes.ItsuggeststhatmicroinjectionofIgGmayinjuretheoocyteandhavesomeeffectsonoocytematuration.Inourexperiments,thefirstpolarbodyemissionisnottotallyinhibitedbyPyk2antibodymicroinjection,thismaybecausePyk2isnotakeyregulatorforF-actinorsomeotherproteinsorkinasesparticipateinregulatingF-actintogetherwithPyk2.Recently,p-MARCK(myristoylatedalanine-richC-kinasesubstrate,amajorsubstrateforproteinkinaseC,isfoundtoenrichintheperipheryoftheactincapoverlyingtheMIorMIIspindletoformaringshapedsubdomain(Michautetal.2005.