SCI结果写作技巧.docx

SCI结果写作技巧.docx

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SCI结果写作技巧

一、结果小标题

1.MEKinhibitorsdecreaseconstitutiveERKactivityandcauseNSCLCcellcyclearrestinG0/G1

2.Flowcytometryassays

3.EffectofZnf179knockdownonthecellcycleprofileandcellcycleregulatoryproteins.

4.Flowcytometricanalysisofcellcycledistribution

5.EffectofLY294002,PD98059andrapamycin,onthegrowthofgag-IR/IGFR-RIE,andv-Src-RIEcellsinmonolayer

6.EffectsofONOO-onmorphologicalchangesinFaDucells

7.EffectsofcurcuminonmorphologicalchangesinFaDucells

8.EffectsofONOO-oncellviabilityinFaDucells

9.CurcumininhibitscellviabilityofFaDucells

10.MEK1inhibitionsuppressesERK1/2activationandinhibitsmyelomacellproliferation

11.CurcumininhibitsthemigrationandinvasionabilityofFaDucells

12.TargetedNOX4knockdownwithaspecificsiRNAblocksRMF-EGstimulusonMCF-7migration

13.EGFR-MSCdemonstratedenhancedmigratoryresponsestowardtumor-conditionedmedia,whichwaspartiallydependentonEGFandEGFR

14.EffectsofcurcuminonmRNAexpressionsofmetastasis-relatedgenesinFaDucells

15.EffectsofONOO-onmRNAexpressionofPDCD4inFaDucells

16.DownregulationofTbRIIinnon-small-celllungcancers（NSCLC）

17.KLF8PromotesCellProliferationandInvasion,InhibitsApoptosis,andInducesEMTInVitro

18.

二、结果开头句

评估….效果，运用….方法，在…..药物浓度以及在….时间作用于….细胞

1.Inordertoassessthecytotoxiceffectofcurcuminonthehumanhypopharyngealcancercells,FaDucellswereculturedwithcurcuminatfinalconcentrationsof10,20,40,60,80,and100µMfor24h,48h,and72h,then,MTTassaywascarriedoutrespectively.

2.MorphologicalchangesofFaDucellswereexaminedbyacridineorangecytochemistrystainingat48haftertreatmentwithcurcuminat0,20,40,60,and80µM,respectively.

3.TheFaDucellcycledistributionwasanalyzedbyMultiCyclesoftwaretoinvestigatetheeffectofcurcuminonthecellcycle.

4.Theanti-metastaticactivityofcurcuminwasevaluatedusingtheTranswellassay.

5.Western-blotanalyseswereperformedtoestimatethelevelsofcaspase-9,caspase-3,Bcl-2andBaxinFaDucellsinresponsetocurcumin（Fig.5A）.

6.AscurcumininhibitedFaDucellsmigrationandinvasion,wefurtherinvestigateditseffectonthemetastasis-relatedgenes.

7.SGNswereexposedtoONOO（100mM）for24hwithorwithoutcurcuminpretreatment.

8.Todelineate描绘thesignalingpathwayofmitochondria-relatedapoptosis,weinvestigatedtheeffectofONOOontheactivationofApaf-1,Caspase-9andCaspase-3geneswhichareinvolvedinthemitochondrion-dependentapoptosispathway.

9.FlowcytometryassaywithAnnexinV/PIdoublestainingshowedthattheapoptoticrateincreasedto50.273.26%aftertreatingwith100mMONOOfor24h（Fig.4B）

10.ToexaminetheroleofZnf179inthecellcycleduringneuronaldifferentiation,P19cellswereinfectedwithnegativecontrolandZnf179shRNAviruses.

11.WeevaluatedcellcycleparametersafterPD98059modulationinfourcases:

threecaseshadhighERKactivity,onecasewasERKnegative.

12.TostudythepossibleroleofNOX4intheRMF-EG-mediatedstimulusonMCF-7migration,weusedatargetedknockdownbysiRNAapproachtodownregulatetheenzymeexpression.

13.InordertotestourhypothesisthatEGFRtransfectionenhancesmigrationofMSCstowardtumorcells,weemployedinvitrotwo-chambermigrationassays.First,wetestedmigratoryresponsesofprimaryMSCsandEGFR-MSCtowardGL261-orB16-conditionedmedia（Fig2a）.

14.RT-PCRanalysesofthemRNAlevelsofPDCD4wereperformedtoestimatetheeffectofONOO-ontheexpressionofPDCD4gene（Fig.4A）.

15.ToverifywhetherTbRIIlevelisdownregulatedinhumanlungtumours,weanalysedforTbRIIexpressionbyRT-PCRusingRNAsamplesfrom46lungtumourspecimens（20squamouscellcarcinoma,19adenocarcinoma,andsevenlargecellcarcinoma）（Figure1A）.

16.SinceweobservedreducedTbRIImRNAexpressioninNSCLC,wetestedtheexpressionofTbRIIproteininlysatesmadefromtumourspecimensbyWesternblotanalysis.

17.The1DCa2mRNAlevelduringheartdevelopmentwascarriedoutbyreal-timeRT-PCRusingtotalRNAisolatedfromfetal,neonatal,4-to6-wk-old,and6-to8-mo-oldratatrialtissueusing18SrRNAasaninternalcontrol.

18.ToinvestigatetheeffectofKLF8inEMT,theexpressionofEMTmarkerswereassessed.

19.Astelomeraseactivityisfrequentlyproposedtobeassociatedwiththemalignantphenotype,weattemptedtodeterminewhetheracorrelationexistsbetweenionizingradiation-inducedcelldeathandtelomeraseactivity.

20.goodCellscollectedatdifferenttimespostirradiationwereexaminedforcellviabilityandtelomeraseactivity.

21.Sincecellsinanactivelygrowingtumoraredistributedindifferentcyclestages,wealsoattemptedtodeterminewhetherthetelomeraseactivityinexponentiallygrowingcellsisaffectedbyionizingradiation.WefirstevaluatedcellcyclephasedistributionsingrowingHeLacellsbyflowcytometry.

22.InviewofthecorrelationbetweentheexpressionstatusofhTERTandtelomeraseactivity

三、结果表达：

1.主系表结构：

结果做主语

主语——thetrend,theproportions,thepercentages,thelevels,theexpressions,cellviability,therate,theactivity,

系表/谓语——1）wassignificantlyobserved，

2）升高increasedto50.273.26%，weregraduallyincreased，wereincreasedsignificantly，wassignificantlyhigher，wasamplifiedinglomeruli，

3）降低werereduced，werereducedto73.41±5.84%，wasdecreasedobviously，wasmarkedlyreducedto，wereunabletogenerateamigratorystimulusonMCF-7cells，

程度副词——用于有显著性统计学差异的词significantly，markedly，remarkably；用于形态学表现有差异的词obviously，apparently；其他gradually逐渐地；mildly轻微地,greatly,appreciably可感觉到地；

1）AsshowninFigure1,atrendofdecreasingviabilitywithincreasingcurcuminconcentrationandexpendingtreatment-timewassignificantlyobservedinFaDucells,indicatingthatcurcumininhibitedthecellviabilityinadose-andtime-dependentmanner.

2）FaDucellsweretreatedwith0,20,and40µMcurcuminfor48hrespectively,asshowninFigure3,theproportionsofFaDucellsinSphaseweregraduallyincreased（from14.23±2.17%to23.10±2.33%,35.13±3.35%）,meanwhile,thepercentagesofcellsatG0/G1phaseweregraduallydecreased（from67.03±3.37%to57.87±4.60%,46.10±2.98%,）.

3）Thelevelsofmigrationwerereducedto73.41±5.84%and58.54±7.76%ofthecontrol（freshmediumalone）levelat10and20µMcurcumin,respectively（Fig.4A,C）.

4）Theproteinexpressionsofcleavedcaspase-9（37kDa）,cleavedcaspase-3（17kDa）andBaxwereincreasedsignificantlyinFaDucellstreatedwithcurcumin20µMand40µMcomparedtothatofcontrol（Fig.5B）,while,theexpressionofBcl-2genewasdecreasedobviously（Fig.5B）,aswellasthedecreaseofratioofBcl-2/Bax（datanotshown）.

5）mRNAexpressionlevelsofpro-metastasisgenesMMP-2andMMP-9werereduced,whereas,Ecadherin,ananti-metastasisgene,wasincreasedsignificantlybycurcuminatconcentrationsof10µMand20µM,respectively（Fig.6）

6）Cellviabilitywasmarkedlyreducedtoapproximately59.573.02%afterexposureto100mMONOOfor24hcomparedwiththecontrolgroup（Fig.2A,B）.

7）FlowcytometryassaywithAnnexinV/PIdoublestainingshowedthattheapoptoticrateincreasedto50.273.26%aftertreatingwith100mMONOOfor24h（Fig.4B）,while,theratesofcontrolgroupandcurcumin-pretreatedgroupwere4.361.47%and7.310.99%respectively（Fig.4A,C）.StatisticanalysesrevealedthattheapoptoticratewassignificantlyhigherinONOOgroupcomparedwiththatincontrolgroup（Fig.4D,p0.001）,andthattheapoptoticrateinthecurcumin-pretreatedgroupdeclinedremarkablyincomparisonwiththatinONOOgroup（Fig.4D,p0.001）.

8）TheSODactivityoftheONOO-treatedgroupwas36.783.62%ofthecontrolgroup.InSGNspretreatedwithcurcumin（15mM,12h）,thelevelofSODactivitywas86.193.13%ofcontrolgroup.

9）ThemRNAexpressionofApaf-1,Caspase-9,Caspase-3wasincreasedsignificantlyafterexposuretoONOO100mMfor24hasevidencedbyRT-PCR（Fig.7）.Moreover,themRNAexpressionofanti-apoptoticgeneBcl-2wasdecreasedwhiletheBaxgeneexpressionwasincreased.

10）AsFigure3Ashows,RMF-EGcellstransientlytransfectedwithsiNOX4wereunabletogenerateamigratorystimulusonMCF-7cells.

11）TheTbRIIexpressionwasfoundtobedecreasedin80%ofsquamouscellcarcinoma,42%adenocarcinoma,and72%largecellcarcinoma

12）ThePCRproductforHip1wasamplifiedinglomeruli,whereasnoexpressionwasdetectedintherestofthekidneylackingglomeruli（Figure1）.

13）ThereactionintensityoftheimmunocytochemistrywasinaccordancewithqRTPCRandWesternblotanalyses

14）Weexamined2hallmarksofEMT:

E-cadherinwasdown-regulated,andVimentinwasup-regulatedinHCCcelllineswithhighKLF8-expressionbyqRT-PCRanalysis.

15）Down-regulationofmesenchymalmarkers（N-cadherin,vimentin,andfibronectin）andupregulationoftheepithelialmarker（E-cadherin）wereobservedbyRT-PCRafterKLF8siRNA（Figure2F）.

16）GoodThesurvivalofdifferentcelltypesafterexposuretogradeddosesofradiation（137Cesiumgammaraysat1.1Gy/min）asdeterminedbyclonogenicassayisshowninFig.1.

17）GoodAstrongcorrelationwasdetectedbetweentelomeraseactivityandcellviabilityinHeLacellstreatedwithionizingradiation.

18）GoodAsimilarpatternofdecreaseintelomeraseactivity（Fig.6）andcellviability（datanotshown）withanincreaseinradiationdosetreatmentwasobservedinthecolorectalcarcinoma（RKO）cellline.

19）Telomeraseactivityisdetectableinmosthumantumortissueandintumor-derivedcelllines,butnotinnormalcellsadjacenttotumorsormixedwithtumor-derivedcelllines（29）.

20）

2.主谓宾结构：

药物做主语

主语——药物名称curcumin，treatment,pr