质粒载体图谱大全Vector.docx
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质粒载体图谱大全Vector
Vector
PDF
Se-
quence
Text
Gene
Bank
Promotor
Marker
Tags
Protease
cleavage
site
Origin
Author
pBAD/MCS
map
seq
gb
araBAD
Amp
none
none
pUC
A.Geerlof
pBADM-11
map
seq
gb
araBAD
Amp
N-His
TEV
pUC
A.Geerlof
pBADM-20
map
seq
gb
araBAD
Amp
N-TrxA
N-His
TEV
pUC
A.Geerlof
pBADM-20(+)
map
seq
gb
araBAD
Amp
N-TrxA
N-His
TEV
pUC
A.Geerlof
pBADM-30
map
seq
gb
araBAD
Amp
N-His
N-GST
TEV
pUC
A.Geerlof
pBADM-30(+)
map
seq
gb
araBAD
Amp
N-His
N-GST
TEV
pUC
A.Geerlof
pBADM-40(+)
map
seq
gb
araBAD
Amp
N-MBP
TEV
pUC
A.Geerlof
pBADM-41(+)
map
seq
gb
araBAD
Amp
N-His
N-MBP
TEV
pUC
A.Geerlof
pBADM-52
map
seq
gb
araBAD
Amp
N-leader-
less-
DsbA
N-His
TEV
pUC
A.Geerlof
pBADM-52(+)
map
seq
gb
araBAD
Amp
N-leader-
less-
DsbA
N-His
TEV
pUC
A.Geerlof
pBADM-60
map
seq
gb
araBAD
Amp
N-NusA
N-His
TEV
pUC
A.Geerlof
pBADM-60(+)
map
seq
gb
araBAD
Amp
N-NusA
N-His
TEV
pUC
A.Geerlof
pBAT4
map
seq
gb
T7
Amp
none
none
pUC
M.Hyvönen
pBAT5
map
seq
gb
T7
Amp
none
none
pUC
M.Hyvönen
pETM-10
map
seq
gb
T7-lac
Kan
N-His
C-His
none
pBR322
G.Stier
pETM-11
map
seq
gb
T7-lac
Kan
N-His
C-His
TEV
pBR322
G.Stier
pETM-12
map
seq
gb
T7
Kan
N-His
Thr
pBR322
G.Stier
pETM-13
map
seq
gb
T7-lac
Kan
none
none
pBR322
G.Stier
pETM-20
map
seq
gb
T7-lac
Amp
N-ThxA
N-His
C-His
TEV
pBR322
G.Stier
pETM-21
map
seq
gb
T7-lac
Amp
N-TrxA
C-His
Thr
pBR322
G.Stier
pETM-30
map
seq
gb
T7-lac
Kan
N-His
N-GST
C-His
TEV
pBR322
G.Stier
pETM-40
map
seq
gb
T7
Kan
N-MBP
TEV
pBR322
G.Stier
pETM-41
map
seq
gb
T7
Kan
N-His
N-MBP
TEV
pBR322
G.Stier
pETM-50
map
seq
gb
T7-lac
Kan
(signal
sequence)
N-DsbA
N-His
C-His
TEV
pBR322
G.Stier
pETM-51
map
seq
gb
T7-lac
Kan
(signal
sequence)
N-DsbA
N-His
C-His
Thr
pBR322
G.Stier
pETM-52
map
seq
gb
T7-lac
Kan
N-DsbA
N-His
C-His
TEV
pBR322
G.Stier
pETM-60
map
seq
gb
T7-lac
Kan
N-NusA
N-His
C-His
TEV
pBR322
G.Stier
pETM-70
map
seq
gb
T7-lac
Kan
N-CBP
C-His
TEV
pBR322
A.Geerlof
pETM-80
map
seq
gb
T7-lac
Kan
(signal
sequence)
N-DsbC
N-His
C-His
TEV
pBR322
G.Stier
pETM-82
map
seq
gb
T7
Kan
N-DsbC
N-His
C-His
TEV
pBR322
G.Stier
pGAT
map
seq
gb
T7
Amp
N-His
N-GST
Thr
pUC
M.Hyvönen
pGAT2
map
seq
gb
T7
Amp
N-His
N-GST
Thr
pUC
M.Hyvönen
pHAT
map
seq
gb
T7-lac
Amp
N-His
none
pUC
M.Hyvönen
pHAT2
map
seq
gb
T7-lac
Amp
N-His
none
pUC
M.Hyvönen
Mostusedantibioticsandtheireffectiveconcentrations
BacktoEscherichiacoliVectors-VectorFeaturespage
Antibiotic
concentration(g/ml)
Amp
ampicillin
100
Cab
carbenicillin
100
Cam
chloramphenicol
34
Kan
kanamycin
30
Rif
rifampicin
200
Tet
tetracyclin
12.5
Escherichiacoliexpressionvectors
Vector
Vectormap
Promoter
Antibioticresistance
Tag(s)
Proteasecleavagesite
Expressionhost
Originofreplication
Source
Description
pACYCDuet-1
map
T7/lac
Cam
N-His
BL21(DE3)
p15A
Novagen
expressionvector;co-expressionof2targetgenes
pACYC-11
map
T7/lac
Cam
N-His
TEV
BL21(DE3)
p15A
EMBL-AG
expressionvector
pACYC-13
map
T7/lac
Cam
C-His
TEV
BL21(DE3)
p15A
EMBL-AG
expressionvector
pACYC-31
map
T7/lac
Cam
N-His
N-GST
TEV
BL21(DE3)
p15A
EMBL-AG
expressionvector
pASK75
tet
Amp
Strep
BL21
Biometra
expressionvector
pBAD/HisA
map
araBAD
Amp
N-His
EK
Top10
LMG194
pUC
Invitrogen
expressionvector
pBAD/HisB
map
araBAD
Amp
N-His
EK
Top10
LMG194
pUC
Invitrogen
expressionvector
pBAD/HisC
map
araBAD
Amp
N-His
EK
Top10
LMG194
pUC
Invitrogen
expressionvector
pBAD/mcs
map
araBAD
Amp
N-His
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-11
map
araBAD
Amp
N-His
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-20
map
araBAD
Amp
N-TrxA
N-His
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-20(+)
map
araBAD
Amp
N-TrxA
N-His
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-30
map
araBAD
Amp
N-His
N-GST
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-30(+)
map
araBAD
Amp
N-His
N-GST
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-40(+)
map
araBAD
Amp
N-MBP
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-41(+)
map
araBAD
Amp
N-His
N-MBP
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-52
map
araBAD
Amp
N-leaderless-DsbA
N-His
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-52(+)
map
araBAD
Amp
N-leaderless-DsbA
N-His
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-60
map
araBAD
Amp
N-NusA
N-His
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBADM-60(+)
map
araBAD
Amp
N-NusA
N-His
TEV
Top10
LMG194
pUC
EMBL-AG
expressionvector
pBAT4
map
T7
Amp
BL21(DE3)
pUC
EMBL-MH
expressionvector
pBAT5
map
T7
Amp
BL21(DE3)
pUC
EMBL-MH
expressionvector
pCal-n
map
T7/lac
Amp
CBP
thrombin
BL21(DE3)
ColE1
Stratagene
expressionvector
pCDF-1b
map
T7/lac
Spec/Strep
N-His
EK
BL21(DE3)
CDF
Novagen
expressionvector
pCDF-11
map
T7/lac
Spec/Strep
N-His
TEV
BL21(DE3)
CDF
EMBL-AG
expressionvector
pCDF-13
map
T7/lac
Spec/Strep
C-His
TEV
BL21(DE3)
CDF
EMBL-AG
expressionvector
pCDFDuet-1
map
T7/lac
Spec/Strep
N-His
BL21(DE3)
CDF
Novagen
expressionvector;co-expressionof2targetgenes
pET-3a
map
T7
Amp
BL21(DE3)
pBR322
Novagen
expressionvector
pET-3b
map
T7
Amp
BL21(DE3)
pBR322
Novagen
expressionvector
pET-3c
map
T7
Amp
BL21(DE3)
pBR322
Novagen
expressionvector
pET-3d
map
T7
Amp
BL21(DE3)
pBR322
Novagen
expressionvector
pET-12a
map
T7
Amp
leadersequence
BL21(DE3)
pBR322
Novagen
expressionvector
pET-14b
map
T7
Amp
N-His
Thrombin
BL21(DE3)
pBR322
Novagen
expressionvector
pET-15b
map
T7/lac
Amp
N-His
Thrombin
BL21(DE3)
pBR322
Novagen
expressionvector
pET-16b
map
T7/lac
Amp
N-His10
FactorXa
BL21(DE3)
pBR322
Novagen
expressionvector
pET-19b
map
T7/lac
Amp
N-His
EK
BL21(DE3)
pBR322
Novagen
expressionvector
pET-20b(+)
map
T7
Amp
leadersequence
C-His
BL21(DE3)
pBR322
Novagen
expressionvectorforsecretedproteins
pET-21d(+)
map
T7/lac
Amp
C-His
BL21(DE3)
pBR322
Novagen
expressionvector
pET-22b(+)
map
T7/lac
Amp
leadersequence
C-His
BL21(DE3)
pBR322
Novagen
expressionvectorforsecretedproteins
pET-24a(+)
map
T7/lac
Kan
C-His
BL21(DE3)
pBR322
Novagen
expressionvector
pET-24d(+)
map
T7/lac
Kan
C-His
BL21(DE3)
pBR322
Novagen
expressionvector
pET-28a(+)
map
T7/lac
Kan
N-His
C-His
Thrombin
BL21(DE3)
pBR322
Novagen
expressionvector
pET-28c(+)
map
T7/lac
Kan
N-His
C-His
Thrombin
BL21(DE3)
pBR322
Novagen
expressionvector
pET-32a(+)
map
T7/lac
Amp
N-TrxA
N-His
C-His
Thrombin
EK
AD494(DE3)
Origami (DE3)
pBR322
Novagen
expressionvectorfordisulfitebondcontainingproteins
pET-32b(+)
map
T7/lac
Amp
N-TrxA
N-His
C-His
Thrombin
EK
AD494(DE3)
Origami (DE3)
pBR322
Novagen
expressionvectorfordisulfitebondcontainingproteins
pET-32c(+)
map
T7/lac
Amp
N-TrxA
N-His
C-His
Thrombin
EK
AD494(DE3)
Origami (DE3)
pBR322
Novagen
expressionvectorfordisulfitebondcontainingproteins
pET-39b(+)
map
T7/lac
Kan
N-DsbA
C-His
Thrombin
EK
BL21(DE3)
pBR322
Novagen
expressionvectorforsecretedproteins
pET-40b(+)
map
T7/lac
Kan
N-DsbC
N-His
C-His
Thrombin
EK
BL21(DE3)
pBR322
Novagen
expressionvectorforsecretedproteins
pETcoco-1
map
T7/lac
Cam
N-His
EK
BL21(DE3)
oriS
oriV
Novagen
expressionvector;allowssingleandmediumvectorcopynumber
pETDuet-1
map
T7/lac
Amp
N-His
BL21(DE3)
pBR322
expressionvector; co-expressionof2targetgenes
pETDsbA-1b
map
T7/lac
Kan
N-LL-DsbA
N-His
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d,leaderlessDsbAforcytoplasmicexpression
pETDsbC-1b
map
T7/lac
Kan
N-LL-DsbC
N-His
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d,leaderlessDsbCforcytoplasmicexpression
pETGB-1a
map
T7/lac
Kan
N-His
N-GB1
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETGB-1b
map
T7/lac
Kan
N-GB1
N-His
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETGST-1a
map
T7/lac
Kan
N-His
N-GST
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETGST-1b
map
T7/lac
Kan
N-GST
N-His
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETMBP-1a
map
T7/lac
Kan
N-His
N-MBP
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETMBP-1b
map
T7/lac
Kan
N-MBP
N-His
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETNusA-1a
map
T7/lac
Kan
N-His
N-NusA
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETNusA-1b
map
T7/lac
Kan
N-NusA
N-His
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETTrxA-1a
map
T7/lac
Kan
N-His
N-TrxA
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETTrxA-1b
map
T7/lac
Kan
N-TrxA
N-His
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETZfl-1b
map
T7/lac
Kan
N-Z-tag(fl)
N-His
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETZ2-1a
map
T7/lac
Kan
N-His
N-Z2
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETZ2-1b
map
T7/lac
Kan
N-Z2
N-His
TEV
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETM-10
map
T7/lac
Kan
N-His
C-His
BL21(DE3)
pBR322
EMBL-GS
modifiedpET-24d
pETM-11
map
T7/lac
Kan
N-His
TEV
BL21(DE3)
pB