designed implemented general purpose framework allows collaborator contribute sequencing instruction.docx

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designedimplementedgeneralpurposeframeworkallowscollaboratorcontributesequencinginstruction

High-performanceliquidchromatographicstudyofthereductionofprotectedoxytoceinbysodiuminliquidammonia  OriginalResearchArticle

JournalofChromatographyA,Volume507,16May1990,Pages59-66

A.Péter,F.Lukács,K.Burger,I.Schón,M.Lówand,L.Kisfaludy

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AbstractAbstract|ReferencesReferences

Abstract

Acombinedhigh-performanceliquidchromatographic（HPLC）-electrochemicalmethodwasdevelopedtoinvestigatethemechanismofreductionofprotectedoxytoceinbymetallicsodiuminliquidammonia.Thechangesintheredoxpotentialandconductivityofprotectedoxytoseinsolutionprovidedinformationonthestoichiometryofthereduction.HPLCmethodselaboratedfortheidentificationandselectivedeterminationofthereactionintermediatesandproductswereusedtoanalysethecompoundsformed.Thiscombinedprocedurerevealedthereactionpatheofthereductionprocessesandpermittedoptimizationoftheexperimentalconditionstoincreasetheyieldofoxytocin.

ArticleOutline

•References

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979

Designandevaluationofacoupledmonolithicpreconcentrator-capillaryzoneelectrophoresissystemfortheextractionofimmunoglobulinGfromhumanserum  OriginalResearchArticle

JournalofChromatographyA,Volume1097,Issues1-2,2December2005,Pages171-178

JennyM.Armenta,BingheGu,PaulH.Humble,CraigD.Thulin,MiltonL.Lee

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Abstract

Theanalysisofproteinsinbiologicalfluidsbycapillaryelectrophoresis（CE）isofinterestinclinicalchemistry.However,duetolowanalyteconcentrationsandpoorconcentrationlimitsofdetection（CLOD）,proteinanalysisbythistechniqueisfrequentlychallenging.CouplingpreconcentrationtechniqueswithCEgreatlyimprovestheCLOD.Anon-linepreconcentration-CEmethodthatcanselectivelypreconcentrateanyproteinforwhichanantibodyisavailablewouldbeveryusefulfortheanalysisoflowabundanceproteinsandwouldestablishCEasamajortoolinbiomarkerdiscovery.Toaccomplishthis,thedevelopmentofanon-lineproteinGmonolithicpreconcentrator-CEdeviceisproposed.Togenerateactivegroupsforproteinimmobilization,glycidylmethacrylate（GMA）wasusedtopreparepolymermonoliths.A1.5–2 cmmonolithwascastinsidea75 μmI.D.fusedsilicacapillarythathadpreviouslybeencoatedwithalternatinglayersofnegatively（dextran）andpositively（polybrene）chargedpolymers.ProteinGwascovalentlyboundtoGMA.Monolithsfromdifferentformulationswerepreparedandevaluatedforbindingcapacitytooptimizethemonolithformulationforproteinpreconcentration.Thephysicalpropertiesofthecolumnconsideredbestforpreconcentrationweredeterminedbymercuryintrusionporosimetry.Thetotalporeareawas4.8 m2/g,theaverageporediameterwas3.3 μmandtheporositywas82%.Themonolithhadalowflowresistanceandwasmacroscopicallyhomogeneous.Theeffectivenessofthemonolithtorapidlypreconcentrateproteinsatflowratesashighas10 μL/minwasdemonstratedusinga1.8 μMIgGsolution.Thissystemprovedeffectiveforon-linesampleextraction,clean-up,preconcentration,andCEofIgGinhumanserum.IgGfromdiluted（500and65,000times）humanserumsampleswassuccessfullyanalyzedusingthissystem.Theapproachcanbeappliedtotheon-linepreconcentrationandanalysisofanyproteinforwhichanantibodyisavailable.

ArticleOutline

1.Introduction

2.Experimental

2.1.Chemicals

2.2.Capillaryzoneelectrophoresis

2.3.Monolithicpreconcentratordesignandevaluation

2.3.1.Capillarysurfacedeactivation

2.3.2.Preparationofpolymermonoliths

2.3.3.ImmobilizationofproteinGonpolymermonoliths

2.3.4.Detectionwindowpreparation

2.4.Scanningelectronmicroscopy（SEM）

2.5.Porousproperties

2.6.Capillaryliquidchromatography

2.7.On-linepreconcentration-CZEofIgG

2.8.On-lineextractionandpreconcentrationofIgGfromhumanserum

3.Resultsanddiscussion

3.1.Monolithicpreconcentratordesignandevaluation

3.1.1.Capillarysurfacedeactivation

3.1.2.Monolithpreparation

3.1.3.DeterminationofthephysicalpropertiesofGMAmonolithrod2

3.1.4.Evaluationoftheeffectofspeedofsampleapplicationonproteinadsorption

3.2.Methoddevelopmentforon-linepreconcentration-CEofIgG

3.3.Applicationofthemonolithicpreconcentratortoahumanserumsample

4.Conclusions

Acknowledgements

References

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TheUseofaPortableMuscleToneMeasurementDevicetoMeasuretheEffectsofBotulinumToxinTypeAonElbowFlexorSpasticity  OriginalResearchArticle

ArchivesofPhysicalMedicineandRehabilitation,Volume86,Issue8,August2005,Pages1655-1660

Jia-JinJasonChen,Yi-NingWu,Sheng-ChihHuang,Hsin-MinLee,Yu-LinWang

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Abstract

ChenJ-JJ,WuY-N,HuangS-C,LeeH-M,WangY-L.TheuseofaportablemuscletonemeasurementdevicetomeasuretheeffectsofbotulinumtoxintypeAonelbowflexorspasticity.

Objective

TouseaportablemuscletoneassessmentdevicetomeasurespasticityafterabotulinumtoxintypeA（BTX-A）injection.

Design

Before-aftertrial.

Setting

Hospital.

Participants

Tenchronicstrokepatientswithupper-limbspasticity.

Intervention

BTX-Awasinjectedinthebicepsbrachii.

MainOutcomeMeasures

Thebiomechanicparameters,viscouscomponent,andaveragedviscosityderivedfromtheacquiredreactiveresistanceandangulardisplacements,aswellasthereflexelectromyographicthresholdofbicepsbrachii,wereusedforspasticityevaluation.

Results

Astatisticallysignificantdecreaseinaveragedviscosityandasignificantincreaseinreflexelectromyographicthreshold（P<.05）bothindicatedreductioninspasticityowingtoBTX-Aintervention.Therewasnoclearreflexelectromyographicactivitydetectedatlowerstretchfrequencies.

Conclusions

Ourportabledesignallowsfortheconvenientuseofthedeviceforquantifyingspasticityinclinics.AllquantitativemeasurementssuggestthatBTX-Adecreasesspasticitywithin2weeksofinjection.Ourportablemuscletonemeasurementdevicemaybeusefulfortheclinicalassessmentofelbowflexorspasticity.

ArticleOutline

Methods

Instrument

ExperimentalProcedures

Participants

Treatmentandevaluation

DataAnalysis

Results

AssessmentofMuscleToneFromBiomechanicData

ReflexElectromyographicThreshold

Discussion

Conclusions

Appendix1.BiomechanicModelofJointMovement

References

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981

Retention-timelockedmethodsingaschromatography  ReviewArticle

JournalofChromatographyA,Volume1216,Issue10,6March2009,Pages1624-1629

NestorEtxebarria,OlatzZuloaga,MaitaneOlivares,LuisJ.Bartolomé,PatriciaNavarro

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Abstract

Retentiontimeisoneofthemostimportantchromatographicfeaturesforanalyticalchemistssinceitisthekeyparametertoseparate,identifyandquantifycompoundsofinterestfromcomplexmixtures.Althoughdetectorswithhigher-dimensionalsignalseasetheidentificationofmanycomponents,therearedemandingrequirementsontheretentiontime,particularlywhenhigh-throughputmethodsareconsidered.Inadditiontothis,gaschromatographicelutionshowssignificantrun-to-runvariationsduetofluctuationsintemperatureandpressure,columndegradationormatrixeffects.Inthissense,differentapproacheshavebeendevelopedtominimisethosevariations:

theintroductionofelectronicpneumaticcontrol（EPC）systems,whichallowaveryefficientcontroloftheflowofthecarriergas,theuseofpeakalignmentalgorithmstotreatthechromatograms,ortheuseofretention-timelocking（RTL）.TheRTLisafeatureoftheAgilentChemStationsoftwareavailableforthoseGCinstrumentsequippedwithEPCsystems.Originallyitwasdevelopedtoassuremethodtranslationbutithasextendedtofixtheretentiontimeandtoimplementpeakdeconvolutionalgorithmsanddatabasebuildingandsearchingfacilities.Inthismanuscript,theRTLbasisandpracticalaspectsaresummarisedtogetherwithabriefdescriptionofsomeapplications.

ArticleOutline

1.Introduction

2.Thebasicsofretention-timelocking

3.Casestudy:

analysisofpetroleumbiomarkers

4.Applications

5.Conclusions

Acknowledgements

References

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982

SASDandtheCERN/SPSrun-timecoordinator  OriginalResearchArticle

NuclearInstrumentsandMethodsinPhysicsResearchSectionA:

Accelerators,Spectrometers,DetectorsandAssociatedEquipment,Volume293,Issues1-2,1August1990,Pages385-389

GiulioMorpurgo

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Abstract

StructuredAnalysisandStructureDesign（SASD）providesuswithahandywayofspecifyingtheflowofdatabetweenthedifferentmodules（functionalunits）ofasystem.Buttheformalismlosesitsimmediacywhenthecontrolflowhastobetakenintoaccountaswell.Moreover,duetothelackofappropriatesoftwareinfrastructure,veryoftentheactualimplementationofthesystemdoesnotreflectthemoduledecouplingandindependencesomuchemphasizedatthedesignstage.Inthispapertheru