designed implemented general purpose framework allows collaborator contribute sequencing instruction.docx

1、designed implemented general purpose framework allows collaborator contribute sequencing instructionHigh-performance liquid chromatographic study of the reduction of protected oxytocein by sodium in liquid ammoniaOriginal Research ArticleJournal of Chromatography A, Volume 507, 16 May 1990, Pages 59

2、-66A. Pter, F. Lukcs, K. Burger, I. Schn, M. Lw and, L. KisfaludyClose preview| Related articles|Related reference work articles AbstractAbstract | ReferencesReferences AbstractA combined high-performance liquid chromatographic (HPLC)-electrochemical method was developed to investigate the mechanism

3、 of reduction of protected oxytocein by metallic sodium in liquid ammonia. The changes in the redox potential and conductivity of protected oxytosein solution provided information on the stoichiometry of the reduction. HPLC methods elaborated for the identification and selective determination of the

4、 reaction intermediates and products were used to analyse the compounds formed. This combined procedure revealed the reaction pathe of the reduction processes and permitted optimization of the experimental conditions to increase the yield of oxytocin.Article Outline ReferencesPurchase979Design and e

5、valuation of a coupled monolithic preconcentrator-capillary zone electrophoresis system for the extraction of immunoglobulin G from human serumOriginal Research ArticleJournal of Chromatography A, Volume 1097, Issues 1-2, 2 December 2005, Pages 171-178Jenny M. Armenta, Binghe Gu, Paul H. Humble, Cra

6、ig D. Thulin, Milton L. LeeClose preview| Related articles|Related reference work articles AbstractAbstract | Figures/TablesFigures/Tables | ReferencesReferences AbstractThe analysis of proteins in biological fluids by capillary electrophoresis (CE) is of interest in clinical chemistry. However, due

7、 to low analyte concentrations and poor concentration limits of detection (CLOD), protein analysis by this technique is frequently challenging. Coupling preconcentration techniques with CE greatly improves the CLOD. An on-line preconcentration-CE method that can selectively preconcentrate any protei

8、n for which an antibody is available would be very useful for the analysis of low abundance proteins and would establish CE as a major tool in biomarker discovery. To accomplish this, the development of an on-line protein G monolithic preconcentrator-CE device is proposed. To generate active groups

9、for protein immobilization, glycidyl methacrylate (GMA) was used to prepare polymer monoliths. A 1.52cm monolith was cast inside a 75m I.D. fused silica capillary that had previously been coated with alternating layers of negatively (dextran) and positively (polybrene) charged polymers. Protein G wa

10、s covalently bound to GMA. Monoliths from different formulations were prepared and evaluated for binding capacity to optimize the monolith formulation for protein preconcentration. The physical properties of the column considered best for preconcentration were determined by mercury intrusion porosim

11、etry. The total pore area was 4.8m2/g, the average pore diameter was 3.3m and the porosity was 82%. The monolith had a low flow resistance and was macroscopically homogeneous. The effectiveness of the monolith to rapidly preconcentrate proteins at flow rates as high as 10L/min was demonstrated using

12、 a 1.8M IgG solution. This system proved effective for on-line sample extraction, clean-up, preconcentration, and CE of IgG in human serum. IgG from diluted (500 and 65,000 times) human serum samples was successfully analyzed using this system. The approach can be applied to the on-line preconcentra

13、tion and analysis of any protein for which an antibody is available.Article Outline1. Introduction2. Experimental 2.1. Chemicals2.2. Capillary zone electrophoresis2.3. Monolithic preconcentrator design and evaluation 2.3.1. Capillary surface deactivation2.3.2. Preparation of polymer monoliths2.3.3.

14、Immobilization of protein G on polymer monoliths2.3.4. Detection window preparation2.4. Scanning electron microscopy (SEM)2.5. Porous properties2.6. Capillary liquid chromatography2.7. On-line preconcentration-CZE of IgG2.8. On-line extraction and preconcentration of IgG from human serum3. Results a

15、nd discussion 3.1. Monolithic preconcentrator design and evaluation 3.1.1. Capillary surface deactivation3.1.2. Monolith preparation3.1.3. Determination of the physical properties of GMA monolith rod 23.1.4. Evaluation of the effect of speed of sample application on protein adsorption3.2. Method dev

16、elopment for on-line preconcentration-CE of IgG3.3. Application of the monolithic preconcentrator to a human serum sample4. ConclusionsAcknowledgementsReferencesPurchase980The Use of a Portable Muscle Tone Measurement Device to Measure the Effects of Botulinum Toxin Type A on Elbow Flexor Spasticity

17、Original Research ArticleArchives of Physical Medicine and Rehabilitation, Volume 86, Issue 8, August 2005, Pages 1655-1660Jia-Jin Jason Chen, Yi-Ning Wu, Sheng-Chih Huang, Hsin-Min Lee, Yu-Lin WangClose preview| Related articles|Related reference work articles AbstractAbstract | Figures/TablesFigur

18、es/Tables | ReferencesReferences AbstractChen J-JJ, Wu Y-N, Huang S-C, Lee H-M, Wang Y-L. The use of a portable muscle tone measurement device to measure the effects of botulinum toxin type A on elbow flexor spasticity. ObjectiveTo use a portable muscle tone assessment device to measure spasticity a

19、fter a botulinum toxin type A (BTX-A) injection. DesignBefore-after trial. SettingHospital. ParticipantsTen chronic stroke patients with upper-limb spasticity. InterventionBTX-A was injected in the biceps brachii. Main Outcome MeasuresThe biomechanic parameters, viscous component, and averaged visco

20、sity derived from the acquired reactive resistance and angular displacements, as well as the reflex electromyographic threshold of biceps brachii, were used for spasticity evaluation. ResultsA statistically significant decrease in averaged viscosity and a significant increase in reflex electromyogra

21、phic threshold (P.05) both indicated reduction in spasticity owing to BTX-A intervention. There was no clear reflex electromyographic activity detected at lower stretch frequencies. ConclusionsOur portable design allows for the convenient use of the device for quantifying spasticity in clinics. All

22、quantitative measurements suggest that BTX-A decreases spasticity within 2 weeks of injection. Our portable muscle tone measurement device may be useful for the clinical assessment of elbow flexor spasticity.Article OutlineMethods InstrumentExperimental Procedures ParticipantsTreatment and evaluatio

23、nData AnalysisResults Assessment of Muscle Tone From Biomechanic DataReflex Electromyographic ThresholdDiscussionConclusionsAppendix 1. Biomechanic Model of Joint MovementReferencesPurchase981Retention-time locked methods in gas chromatographyReview ArticleJournal of Chromatography A, Volume 1216, I

24、ssue 10, 6 March 2009, Pages 1624-1629Nestor Etxebarria, Olatz Zuloaga, Maitane Olivares, Luis J. Bartolom, Patricia NavarroClose preview| Related articles|Related reference work articles AbstractAbstract | Figures/TablesFigures/Tables | ReferencesReferences AbstractRetention time is one of the most

25、 important chromatographic features for analytical chemists since it is the key parameter to separate, identify and quantify compounds of interest from complex mixtures. Although detectors with higher-dimensional signals ease the identification of many components, there are demanding requirements on

26、 the retention time, particularly when high-throughput methods are considered. In addition to this, gas chromatographic elution shows significant run-to-run variations due to fluctuations in temperature and pressure, column degradation or matrix effects. In this sense, different approaches have been

27、 developed to minimise those variations: the introduction of electronic pneumatic control (EPC) systems, which allow a very efficient control of the flow of the carrier gas, the use of peak alignment algorithms to treat the chromatograms, or the use of retention-time locking (RTL). The RTL is a feat

28、ure of the Agilent ChemStation software available for those GC instruments equipped with EPC systems. Originally it was developed to assure method translation but it has extended to fix the retention time and to implement peak deconvolution algorithms and database building and searching facilities.

29、In this manuscript, the RTL basis and practical aspects are summarised together with a brief description of some applications.Article Outline1. Introduction2. The basics of retention-time locking3. Case study: analysis of petroleum biomarkers4. Applications5. ConclusionsAcknowledgementsReferencesPur

30、chase982SASD and the CERN / SPS run-time coordinatorOriginal Research ArticleNuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Volume 293, Issues 1-2, 1 August 1990, Pages 385-389Giulio MorpurgoClose preview| Related artic

31、les|Related reference work articles AbstractAbstract | ReferencesReferences AbstractStructured Analysis and Structure Design (SASD) provides us with a handy way of specifying the flow of data between the different modules (functional units) of a system. But the formalism loses its immediacy when the control flow has to be taken into account as well. Moreover, due to the lack of appropriate software infrastructure, very often the actual implementation of the system does not reflect the module decoupling and independence so much emphasized at the design stage. In this paper the ru