常用荧光染料的激发及发射波长.docx

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常用荧光染料的激发及发射波长.docx

常用荧光染料的激发及发射波长

 

常用荧光染料的激发及发射波长(总22页)

荧光素名称

激发波长(nm)

发射波长(nm)

备注

1,5IAEDANS

336

490

1,8-ANS

372

480

4-Methylumbelliferone

385

502

5-carboxy-2,7-dichlorofluorescein

504

529

5-Carboxyfluorescein(5-FAM)

492

518

5-Carboxynapthofluorescein(pH10)

512/598

563/668

RatioDye,pH

5-Carboxytetramethylrhodamine(5-TAMRA)

542

568

5-FAM(5-Carboxyfluorescein)

492

518

5-HAT(HydroxyTryptamine)

370-415

520-540

5-HydroxyTryptamine(HAT)

370-415

520-540

5-ROX(carboxy-X-rhodamine)

578567

604591

5-TAMRA(5-Carboxytetramethylrhodamine)

548542

552568

6-Carboxyrhodamine6G

518

543

6-CR6G

518

543

6-JOE

520

548

7-Amino-4-methylcoumarin

351

430

7-AminoactinomycinD(7-AAD)

546

647

7-Hydroxy-4-methylcoumarin

360

449,455

9-Amino-6-chloro-2-methoxyacridine

412,430

471,474

ABQ

344

445

AcidFuchsin

540

630

ACMA(9-Amino-6-chloro-2-methoxyacridine)

412,430

471,474

AcridineOrange+DNA

502

526

AcridineOrange+RNA

460

650

AcridineOrange,bothDNA&RNA

440-480

520-650

AcridineRed

455-600

560-680

AcridineYellow

470

550

Acriflavin

436

520

AcriflavinFeulgenSITSA

355-425

460

Aequorin(Photoprotein)

466

Photoprotein

AFPs-AutoFluorescentProtein-(QuantumBiotechnologies)seesgGFP,sgBFP

AlexaFluor350?

346342

442441

AlexaFluor430?

431

540

AlexaFluor488?

495,492

519,520

AlexaFluor532?

531,532

553,554

AlexaFluor546?

556,557

572,573

AlexaFluor568?

577,578

603

AlexaFluor594?

590,594

617,618

AlexaFluor633?

632

650

AlexaFluor647?

647

666

AlexaFluor660?

668

698

AlexaFluor680?

679

702

AlizarinComplexon

530-560,580

580,624-645

AlizarinRed

530-560

580

Allophycocyanin(APC)

630,645

655,660

AMC,AMCA-S

345

445

AMCA(Aminomethylcoumarin)

345347

425444

AMCA-X

353

442

AminoactinomycinD

555

655

Aminocoumarin

346350

442445

Aminomethylcoumarin(AMCA)

345347

425444

AnilinBlue

600

Anthrocylstearate

360-381

446

APC(Allophycocyanin)

630,645

655,660

APC-Cy7

625-650

755

APTRA-BTC=RatioDye,Zn2+

466/380

520/530

RatioDye,Zn2+

APTS

424

505

AstrazonBrilliantRed4G

500

585

AstrazonOrangeR

470

540

AstrazonRed6B

520

595

AstrazonYellow7GLL

450

480

Atabrine

436

490

ATTO-TAGCBQCA

465

560

ATTO-TAGFQ

486

591

Auramine

460

550

AurophosphineG

450

580

Aurophosphine

450-490

515

BAO9(Bisaminophenyloxadiazole)

365

395

BCECF(highpH)

492,503

520,528

BCECF(lowpH)

482

520

BerberineSulphate

430

550

BetaLactamase

409

447,520

BFPblueshiftedGFP(Y66H)BlueFluorescentProtein

381,382,383

445,447,448

blueshiftedGFP(Y66H)BlueFluorescentProtein

BFP/GFPFRET

Bimane

398

490

Bisbenzamide

360

461

Bisbenzimide(Hoechst)

360

461

bis-BTC=RatioDye,Zn2+

455/405

529/505

RatioDye,Zn2+

BlancophorFFG

390

470

BlancophorSV

370

435

BOBO-1

462

481

BOBO-3

570

602

Bodipy492/515

490

515

Bodipy493/503

533

549

Bodipy500/510

509

515

Bodipy505/515

502

510

Bodipy530/550

528

547

Bodipy542/563

543

563

Bodipy558/568

558

569

Bodipy564/570

564

570

Bodipy576/589

579

590

Bodipy581/591

584

592

Bodipy630/650-X

625

642

Bodipy650/665-X

647

665

Bodipy665/676

605

676

BodipyFl

504,505

511,513

BodipyFLATP

505

514

BodipyFl-Ceramide

505

511

BodipyR6GSE

528

547

BodipyTMR

542

574

BodipyTMR-Xconjugate

544

573

BodipyTMR-X,SE

544

570

BodipyTR

589

617

BodipyTRATP

591

620

BodipyTR-XSE

588

616

BO-PRO-1

462

481

BO-PRO-3

544

570

BrilliantSulphoflavinFF

430

520

BTC-RatioDyeCa2+

464/401

533/529

RatioDyeCa2+

BTC-5N-atioDye,Zn2+

459/417

517/532

RatioDye,Zn2+

Calcein

494

517

CalceinBlue

373

440

CalciumCrimson?

588,589

611,615

CalciumGreen

501,506

531

CalciumGreen-1Ca2+Dye

506

531

Ca2+Dye

CalciumGreen-2Ca2+

506/503

536

Ca2+

CalciumGreen-5NCa2+

506

532

Ca2+

CalciumGreen-C18Ca2+

509

530

Ca2+

CalciumOrange

549

575576

CalcofluorWhite

385,395,405

437,440,445

Carboxy-X-rhodamine(5-ROX)

576

601

CascadeBlue?

377398399

420423

CascadeYellow

399400

550552

Catecholamine

410

470

CCF2(GeneBlazer)

CFDA

494

520

CFP-CyanFluorescentProtein

430,433,436,(453)

474,475,476,(501)

CyanFluorescentProtein

CFP/YFPFRET

Chlorophyll

480

650

ChromomycinA

436-460

470

ChromomycinA

445

575

CL-NERF(RatioDye,pH)

504/514

540

RatioDye,pH

CMFDA

494

520

CoelenterazineCa2+Dye,bioluminescence

(429)

465

Ca2+Dye,bioluminescence,nativemolecule

Coelenterazinecp(Ca2+Dye,)

(430)

442

Ca2+Dye,bioluminescence

Coelenterazinef

(437)

473

Ca2+Dye,bioluminescence

Coelenterazinefcp

452

Ca2+Dye,bioluminescence

Coelenterazineh

(437)

464

Ca2+Dye,bioluminescence

Coelenterazinehcp

(433)

444

Ca2+Dye,bioluminescence

Coelenterazineip

441

Ca2+Dye,bioluminescence

Coelenterazinen

(431)

467

Ca2+Dye,bioluminescence

CoelenterazineO

460

575

CoumarinPhalloidin

387

470

C-phycocyanine

CPMMethylcoumarin

384

469

Methylcoumarin

CTC

400-450

602

CTCFormazan

Cy2?

489

506

Cy3.18

554

568

Cy3.5?

581

598

Cy3?

514552554

566570

Cy5.18

649

666

Cy5.5?

675

695

Cy5?

649

666670

Cy7?

710,743

767,805

CyanGFP

433(453)

475(501)

cyclicAMPFluorosensor(FiCRhR)

500

517

CyQuantCellProliferationAssay

480

520

CellProliferationAssay

Dabcyl

453

Dansyl

340

578

DansylAmine

337

517

DansylCadaverine

335

518

DansylChloride

372

518

DansylDHPE

336

517

Dansylfluoride

356

none

DAPI

359

461

Dapoxyl

403

580

Dapoxyl2

374

574

Dapoxyl3

373

574

DCFDA

504

529

DCFH(DichlorodihydrofluoresceinDiacetate)

505

535

DDAO

463

607

DHR(Dihydorhodamine123)

505

534

Di-4-ANEPPS

496

705

Di-8-ANEPPS(non-ratio)

488498

605713

DiA(4-Di-16-ASP)

456

591

DichlorodihydrofluoresceinDiacetate(DCFH)

505

535

DiD-LipophilicTracer

644

665

LipophilicTracer

DiD(DiIC18(5))

644

665

DIDS

341

415

Dihydorhodamine123(DHR)

505

535

DiI(DiIC18(3))

549,551

565

Dinitrophenol

349

DiO(DiOC18(3))

484,487

501,502

DiR

748

780

LipophilicTracer

DiR(DiIC18(7))

750

779

DM-NERF(highpH)

497/510

540

RatioDye,pH

DNP

349

Dopamine

340

490-520

DsRed

558

583

Redfluorescentprotein

DTAF

494

520

DY-630-NHS

621

660

HemicyanelabelforproteinsandDNA

DY-635-NHS

634

664

HemicyanelabelforproteinsandDNA

EBFP

383

447

EnhancedBlueFluorescentProtein

ECFP

436

474

EnhancedCyanFluorescentProtein

EGFP

488,498

507,516

EnhancedGreenFluorescentProtein

ELF97

345

530

Eosin

524

545

Erythrosin

529,532

554,555

ErythrosinITC

529

555

EthidiumBromide

510,523

595,605

Ethidiumhomodimer-1(EthD-1)

528

617

Euchrysin

430

540

EukoLight

Europium(III)chloride

EYFP

513,520

527,532

EnhancedYellowFluorescentProtein

FastBlue

360

440

FDA

494

520

Feulgen(Pararosaniline)

570

625

FIF(FormaldehydInducedFluorescence)

405

433

FITC

490,494

520,525

FITCAntibody

493

517

FlazoOrange

375-530

612

Fluo-3

480–506,506

520,527

Fluo-4

494

516

Fluorescein(FITC)

490,494

520,525

FluoresceinDiacetate

494

520

Fluoro-Emerald

495

524

Fluoro-Gold(Hydroxystilbamidine)

361

536

Fluor-Ruby

555

582

FluorX

494

520

FM1-43?

479

598

FM4-46

515

640

FuraRed(highpH)

572

657

FuraRed/Fluo-3

Fura-2,highcalcium

335

505

Excitationratiodye

Fura-2,lowcalcium

363

512

Excitationratiodye

Fura-2/BCECF

GenacrylBrilliantRedB

520

590

GenacrylBrilliantYellow10GF

430

485

GenacrylPink3G

470

583

GenacrylYellow5GF

430

475

GeneBlazer(CCF2)

GFP(S65T)

498

516

GFPredshifted(rsGFP)

498

516

GFPwildtype,non-UVexcitation(wtGFP)

475

509

GFPwildtype,UVexcitation(wtGFP)

395

509

GFPuv

385

508

GloxalicAcid

405

460

GranularBlue

355

425

Haematoporphyrin

530-560

580

Hoechst33258

345

487

Hoechst33342

347

483

Hoechst34580

392

440

HPTS

355

465

Hydroxycoumarin

325-360

386-455

Hydroxystilbamidine(FluoroGold)

361

536

Hydroxytryptamine

400

530

Indo-1,highcalcium

330

401

Emissionratiodye

Indo-1,lowcalcium

346

475

Emissionratiodye

Indodicarbocyanine(DiD)

644

665

Indotricarbocyanine(DiR)

748

780

IntrawhiteCf

360

430

JC-1

514

529

JO-JO-1

530

545

JO-PRO-1

532

544

LaserPro

795

812

Laurodan

355

460

LDS751(DNA)

543

712

LDS751(RNA)

590

607

LeucophorPAF

370

430

LeucophorSF

380

465

LeucophorWS

395

465

LissamineRhodamine

572,577

591,592

LissamineRhodamineB

577

592

LIVE/DEADKitAnimalCellsCalcein/Ethidiumhomodimer

494528

517617

formoredetailsreferto

LOLO-1

566

580

LO-PRO-1

568

581

LuciferYellow

425,428

528,536,540

LysoTrackerBlue

373

422

LysoTrackerBlue-White

466

536

LysoTrackerGreen

504,534

511,551

LysoTrackerRed

490

516

LysoTrackerYellow

551

576

LysoSensorBlue

374

424

LysoSensorGreen

442

505

LysoSensorYellow/Blue

384

540

MagGreen

507

531

MagdalaRed(PhloxinB)

524

600

Mag-FuraRed

483/427

659/631

RatioDye,Mg2+

Mag-Fura-2

369/329369/33

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