常用荧光染料的激发及发射波长.docx

1、常用荧光染料的激发及发射波长常用荧光染料的激发及发射波长(总22页)荧光素名称激发波长 (nm)发射波长 (nm)备注1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein (pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM

2、 (5-Carboxyfluorescein)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)578567 6045915-TAMRA (5-Carboxytetramethylrhodamine)548542 5525686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin

3、 D (7-AAD)5466477-Hydroxy-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471, 474ABQ344445Acid Fuchsin540630ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red45

4、5-600560-680Acridine Yellow470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFPAlexa Fluor 350?346342 442441Alexa Fluor 430?431540Alexa Fluor 488?495, 492519, 520Alexa Fluor 532?531,

5、532553, 554Alexa Fluor 546?556, 557572, 573Alexa Fluor 568?577, 578603Alexa Fluor 594?590, 594617, 618Alexa Fluor 633?632650Alexa Fluor 647?647666Alexa Fluor 660?668698Alexa Fluor 680?679702Alizarin Complexon530-560, 580580, 624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA

6、-S345445AMCA (Aminomethylcoumarin)345347 425444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346350 442445Aminomethylcoumarin (AMCA)345347 425444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+

7、APTS424505Astrazon Brilliant Red 4G500585Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG CBQCA465560ATTO-TAG FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9 (Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low

8、pH)482520Berberine Sulphate430550Beta Lactamase409447, 520BFP blue shifted GFP (Y66H)Blue Fluorescent Protein381, 382, 383445, 447, 448blue shifted GFP (Y66H)Blue Fluorescent ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye

9、, Zn2+Blancophor FFG390470Blancophor SV370435BOBO -1462481BOBO -3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy

10、650/665-X647665Bodipy 665/676605676Bodipy Fl504, 505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO -1462481BO-PRO -3544570Brilliant Sulphoflavin FF430

11、520BTC - Ratio Dye Ca2+464/401533/529Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crimson?588, 589611, 615Calcium Green501, 506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium

12、 Green-C18 Ca2+509530Ca2+Calcium Orange549575576Calcofluor White385, 395, 405437, 440, 445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue?377398399 420423Cascade Yellow399400 550552Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430, 433, 436, (453)474, 475, 476, (501)Cyan

13、Fluorescent ProteinCFP/YFP FRETChlorophyll480650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Coelenterazine Ca2+ Dye, bioluminescence(429)465Ca2+ Dye, bioluminescence, native moleculeCoelenterazine cp (Ca2+ Dye,)(430)442Ca2+ Dye, bioluminescenceC

14、oelenterazine f(437)473Ca2+ Dye, bioluminescenceCoelenterazine fcp452Ca2+ Dye, bioluminescenceCoelenterazine h(437)464Ca2+ Dye, bioluminescenceCoelenterazine hcp(433)444Ca2+ Dye, bioluminescenceCoelenterazine ip441Ca2+ Dye, bioluminescenceCoelenterazine n(431)467Ca2+ Dye, bioluminescenceCoelenterazi

15、ne O460575Coumarin Phalloidin387470C-phycocyanineCPM Methylcoumarin384469MethylcoumarinCTC400-450602CTC FormazanCy2?489506Cy3.1 8554568Cy3.5?581598Cy3?514552554 566570Cy5.1 8649666Cy5.5?675695Cy5?649666670Cy7?710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cel

16、l Proliferation Assay480520Cell Proliferation AssayDabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH (Dichlorodihydrofluorescein Diacetate)505535DDAO463607DHR

17、(Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-ANEPPS (non-ratio)488498 605713DiA (4-Di-16-ASP)456591Dichlorodihydrofluorescein Diacetate (DCFH)505535DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3)549, 551565Dinitrophenol349

18、DiO (DiOC18(3)484, 487501, 502DiR748780Lipophilic TracerDiR (DiIC18(7)750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent proteinDTAF494520DY-630-NHS621660Hemicyanelabel for proteins and DNADY-635-NHS634664Hemicyanelabel for proteins and DNAEBFP383447Enha

19、nced Blue Fluorescent ProteinECFP436474Enhanced Cyan Fluorescent ProteinEGFP488, 498507, 516Enhanced Green Fluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchrysin430540EukoLightEuropium (III

20、) chlorideEYFP513, 520527, 532Enhanced Yellow Fluorescent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480 506, 506520, 527Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluo

21、rescein Diacetate494520Fluoro-Emerald495524Fluoro-Gold (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43?479598FM 4-46515640Fura Red (high pH)572657Fura Red/Fluo-3Fura-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Re

22、d B520590Genacryl Brilliant Yellow 10GF430485Genacryl Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematopo

23、rphyrin530-560580Hoechst 33258345487Hoechst 33342347483Hoechst 34580392440HPTS355465Hydroxycoumarin325-360386-455Hydroxystilbamidine (FluoroGold)361536Hydroxytryptamine400530Indo-1, high calcium330401Emission ratio dyeIndo-1, low calcium346475Emission ratio dyeIndodicarbocyanine (DiD)644665Indotrica

24、rbocyanine (DiR)748780Intrawhite Cf360430JC-1514529JO-JO-1530545JO-PRO-1532544LaserPro795812Laurodan355460LDS 751 (DNA)543712LDS 751 (RNA)590607Leucophor PAF370430Leucophor SF380465Leucophor WS395465Lissamine Rhodamine572, 577591, 592Lissamine Rhodamine B577592LIVE/DEAD Kit Animal CellsCalcein/Ethid

25、ium homodimer494528517617for more details referto LOLO-1566580LO-PRO-1568581Lucifer Yellow425, 428528, 536, 540Lyso Tracker Blue373422Lyso Tracker Blue-White466536Lyso Tracker Green504, 534511, 551Lyso Tracker Red490516Lyso Tracker Yellow551576LysoSensor Blue374424LysoSensor Green442505LysoSensor Yellow/Blue384540Mag Green507531Magdala Red (Phloxin B)524600Mag-Fura Red483/427659/631Ratio Dye, Mg2+Mag-Fura-2369/329369/33