实验四.docx

实验四.docx

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实验四

核酸序列NM_000230Fasta格式

>gi|169790920|ref|NM_000230.2|Homosapiensleptin（LEP）,mRNA

GTAGGAATCGCAGCGCCAGCGGTTGCAAGGCCCAAGAAGCCCATCCTGGGAAGGAAAATGCATTGGGGAA

CCCTGTGCGGATTCTTGTGGCTTTGGCCCTATCTTTTCTATGTCCAAGCTGTGCCCATCCAAAAAGTCCA

AGATGACACCAAAACCCTCATCAAGACAATTGTCACCAGGATCAATGACATTTCACACACGCAGTCAGTC

TCCTCCAAACAGAAAGTCACCGGTTTGGACTTCATTCCTGGGCTCCACCCCATCCTGACCTTATCCAAGA

TGGACCAGACACTGGCAGTCTACCAACAGATCCTCACCAGTATGCCTTCCAGAAACGTGATCCAAATATC

CAACGACCTGGAGAACCTCCGGGATCTTCTTCACGTGCTGGCCTTCTCTAAGAGCTGCCACTTGCCCTGG

GCCAGTGGCCTGGAGACCTTGGACAGCCTGGGGGGTGTCCTGGAAGCTTCAGGCTACTCCACAGAGGTGG

TGGCCCTGAGCAGGCTGCAGGGGTCTCTGCAGGACATGCTGTGGCAGCTGGACCTCAGCCCTGGGTGCTG

AGGCCTTGAAGGTCACTCTTCCTGCAAGGACTACGTTAAGGGAAGGAACTCTGGCTTCCAGGTATCTCCA

GGATTGAAGAGCATTGCATGGACACCCCTTATCCAGGACTCTGTCAATTTCCCTGACTCCTCTAAGCCAC

TCTTCCAAAGGCATAAGACCCTAAGCCTCCTTTTGCTTGAAACCAAAGATATATACACAGGATCCTATTC

TCACCAGGAAGGGGGTCCACCCAGCAAAGAGTGGGCTGCATCTGGGATTCCCACCAAGGTCTTCAGCCAT

CAACAAGAGTTGTCTTGTCCCCTCTTGACCCATCTCCCCCTCACTGAATGCCTCAATGTGACCAGGGGTG

ATTTCAGAGAGGGCAGAGGGGTAGGCAGAGCCTTTGGATGACCAGAACAAGGTTCCCTCTGAGAATTCCA

AGGAGTTCCATGAAGACCACATCCACACACGCAGGAACTCCCAGCAACACAAGCTGGAAGCACATGTTTA

TTTATTCTGCATTTTATTCTGGATGGATTTGAAGCAAAGCACCAGCTTCTCCAGGCTCTTTGGGGTCAGC

CAGGGCCAGGGGTCTCCCTGGAGTGCAGTTTCCAATCCCATAGATGGGTCTGGCTGAGCTGAACCCATTT

TGAGTGACTCGAGGGTTGGGTTCATCTGAGCAAGAGCTGGCAAAGGTGGCTCTCCAGTTAGTTCTCTCGT

AACTGGTTTCATTTCTACTGTGACTGATGTTACATCACAGTGTTTGCAATGGTGTTGCCCTGAGTGGATC

TCCAAGGACCAGGTTATTTTAAAAAGATTTGTTTTGTCAAGTGTCATATGTAGGTGTCTGCACCCAGGGG

TGGGGAATGTTTGGGCAGAAGGGAGAAGGATCTAGAATGTGTTTTCTGAATAACATTTGTGTGGTGGGTT

CTTTGGAAGGAGTGAGATCATTTTCTTATCTTCTGCAATTGCTTAGGATGTTTTTCATGAAAATAGCTCT

TTCAGGGGGGTTGTGAGGCCTGGCCAGGCACCCCCTGGAGAGAAGTTTCTGGCCCTGGCTGACCCCAAAG

AGCCTGGAGAAGCTGATGCTTTGCTTCAAATCCATCCAGAATAAAACGCAAAGGGCTGAAAGCCATTTGT

TGGGGCAGTGGTAAGCTCTGGCTTTCTCCGACTGCTAGGGAGTGGTCTTTCCTATCATGGAGTGACGGTC

CCACACTGGTGACTGCGATCTTCAGAGCAGGGGTCCTTGGTGTGACCCTCTGAATGGTCCAGGGTTGATC

ACACTCTGGGTTTATTACATGGCAGTGTTCCTATTTGGGGCTTGCATGCCAAATTGTAGTTCTTGTCTGA

TTGGCTCACCCAAGCAAGGCCAAAATTACCAAAAATCTTGGGGGGTTTTTACTCCAGTGGTGAAGAAAAC

TCCTTTAGCAGGTGGTCCTGAGACCTGACAAGCACTGCTAGGCGAGTGCCAGGACTCCCCAGGCCAGGCC

ACCAGGATGGCCCTTCCCACTGGAGGTCACATTCAGGAAGATGAAAGAGGAGGTTTGGGGTCTGCCACCA

TCCTGCTGCTGTGTTTTTGCTATCACACAGTGGGTGGTGGATCTGTCCAAGGAAACTTGAATCAAAGCAG

TTAACTTTAAGACTGAGCACCTGCTTCATGCTCAGCCCTGACTGGTGCTATAGGCTGGAGAAGCTCACCC

AATAAACATTAAGATTGAGGCCTGCCCTCAGGGATCTTGCATTCCCAGTGGTCAAACCGCACTCACCCAT

GTGCCAAGGTGGGGTATTTACCACAGCAGCTGAACAGCCAAATGCATGGTGCAGTTGACAGCAGGTGGGA

AATGGTATGAGCTGAGGGGGGCCGTGCCCAGGGGCCCACAGGGAACCCTGCTTGCACTTTGTAACATGTT

TACTTTTCAGGGCATCTTAGCTTCTATTATAGCCACATCCCTTTGAAACAAGATAACTGAGAATTTAAAA

ATAAGAAAATACATAAGACCATAACAGCCAACAGGTGGCAGGACCAGGACTATAGCCCAGGTCCTCTGAT

ACCCAGAGCATTACGTGAGCCAGGTAATGAGGGACTGGAACCAGGGAGACCGAGCGCTTTCTGGAAAAGA

GGAGTTTCGAGGTAGAGTTTGAAGGAGGTGAGGGATGTGAATTGCCTGCAGAGAGAAGCCTGTTTTGTTG

GAAGGTTTGGTGTGTGGAGATGCAGAGGTAAAAGTGTGAGCAGTGAGTTACAGCGAGAGGCAGAGAAAGA

AGAGACAGGAGGGCAAGGGCCATGCTGAAGGGACCTTGAAGGGTAAAGAAGTTTGATATTAAAGGAGTTA

AGAGTAGCAAGTTCTAGAGAAGAGGCTGGTGCTGTGGCCAGGGTGAGAGCTGCTCTGGAAAATGTGACCC

AGATCCTCACAACCACCTAATCAGGCTGAGGTGTCTTAAGCCTTTTGCTCACAAAACCTGGCACAATGGC

TAATTCCCAGAGTGTGAAACTTCCTAAGTATAAATGGTTGTCTGTTTTTGTAACTTAAAAAAAAAAAAAA

AAGTTTGGCCGGGTGCGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGTGGGGGGATCAC

AAGGTCACTAGATGGCGAGCATCCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAACACAAAAGTTA

GCTGAGCGTGGTGGCGGGCGCCTGTAGTCCCAGCCACTCGGGAGGCTGAGACAGGAGAATCGCTTAAACC

TGGGAGGCGGAGAGTACAGTGAGCCAAGATCGCGCCACTGCACTCCGGCCTGATGACAGAGCGAGATTCC

GTCTTAAAAAAAAAAAAAAAAAAGTTTGTTTTTAAAAAAATCTAAATAAAATAACTTTGCCCCCTGCAAA

AAAAAAAAAAAAAA

核酸序列NM_000230的GenBank格式

LOCUSNM_0002303444bpmRNAlinearPRI25-MAY-2014

DEFINITIONHomosapiensleptin（LEP）,mRNA.

ACCESSIONNM_000230

VERSIONNM_000230.2GI:

169790920

KEYWORDSRefSeq.

SOURCEHomosapiens（human）

ORGANISMHomosapiens

Eukaryota;Metazoa;Chordata;Craniata;Vertebrata;Euteleostomi;

Mammalia;Eutheria;Euarchontoglires;Primates;Haplorrhini;

Catarrhini;Hominidae;Homo.

REFERENCE1（bases1to3444）

AUTHORSLinkovF,BurkeLE,KomaroffM,EdwardsRP,LokshinA,StynMA,

TseytlinE,FreeseKEandBovbjergDH.

TITLEAnexploratoryinvestigationoflinksbetweenchangesinadipokines

andqualityoflifeinindividualsundergoingweightloss

interventions:

possibleimplicationsforcancerresearch

JOURNALGynecol.Oncol.133

（1）,67-72（2014）

PUBMED24462731

REMARKGeneRIF:

Leptinwasassociatedwithphysicalqualityoflifein

overweight/obesewomenundergoingweightlossinterventions.

REFERENCE2（bases1to3444）

AUTHORSWoolleyJD,KhanBK,NatesanA,KarydasA,DallmanM,HavelP,

MillerBLandRankinKP.

TITLESatiety-relatedhormonaldysregulationinbehavioralvariant

frontotemporaldementia

JOURNALNeurology82（6）,512-520（2014）

PUBMED24415571

REMARKGeneRIF:

Onlypatientswithbehavioralvariantfrontotemporal

dementiawhosignificantlyoverateinthelaboratorydemonstrated

significantlyhigherlevelsofleptincomparedwithcontrols.

REFERENCE3（bases1to3444）

AUTHORSHartCN,CarskadonMA,ConsidineRV,FavaJL,LawtonJ,RaynorHA,

JelalianE,OwensJandWingR.

TITLEChangesinchildren'ssleepdurationonfoodintake,weight,and

leptin

JOURNALPediatrics132（6）,E1473-E1480（2013）

PUBMED24190680

REMARKGeneRIF:

Comparedwithdecreasedsleep,increasedsleepdurationin

school-agechildrenresultedinlowerreportedfoodintake,lower

fastingleptinlevels,andlowerweight.

REFERENCE4（bases1to3444）

AUTHORSKleinridders,A.,Lauritzen,H.P.,Ussar,S.,Christensen,J.H.,

Mori,M.A.,Bross,P.andKahn,C.R.

TITLELeptinregulationofHsp60impactshypothalamicinsulinsignaling

JOURNALJ.Clin.Invest.123（11）,4667-4680（2013）

PUBMED24084737

REMARKGeneRIF:

Intype2diabetes,thereishypothalamicinsulin

resistanceandmitochondrialdysfunctionduetodownregulationof

themitochondrialchaperoneHSP60.HSP60reductionwasduetoa

lackofproperleptinsignaling.

REFERENCE5（bases1to3444）

AUTHORSCicchella,A.,Stefanelli,C.,Jurimae,T.,Saar,M.andPurge,P.

TITLEModeratephysicalactivitycorrelateswithelevatedleptinin

physicallyactive10-12-year-oldboyswithnormalBMI

JOURNALPerceptMotSkills117

（2）,358-366（2013）

PUBMED24611241

REMARKGeneRIF:

Moderatephysicalactivitywascorrelatedwithleptin

levelsinphysicallyactive10-12yearoldboys.

REFERENCE6（bases1to3444）

AUTHORSIsseN,OgawaY,TamuraN,MasuzakiH,MoriK,OkazakiT,SatohN,

ShigemotoM,YoshimasaYandNishiS.

CONSRTMetal

TITLEStructuralorganizationandchromosomalassignmentofthehuman

obesegene

JOURNALJ.Biol.Chem.270（46）,27728-27733（1995）

PUBMED7499240

REFERENCE7（bases1to3444）

AUTHORSMasuzakiH,OgawaY,IsseN,SatohN,OkazakiT,ShigemotoM,Mori

K,TamuraN,HosodaKandYoshimasaY.

CONSRTMetal

TITLEHumanobesegeneexpression.Adipocyte-specificexpressionand

regionaldifferencesintheadiposetissue

JOURNALDiabetes44（7）,855-858（1995）

PUBMED7789654

REFERENCE8（bases1to3444）

AUTHORSZhangY,ProencaR,MaffeiM,BaroneM,LeopoldLandFriedmanJM.

TITLEPositionalcloningofthemouseobesegeneanditshumanhomologue

JOURNALNature372（6505）,425-432（1994）

PUBMED7984236

REMARKErratum:

[Nature1995Mar30;374（6521）:

479]

REFERENCE9（bases1to3444）

AUTHORSFriedmanJM,LeibelRL,SiegelDS,WalshJandBaharyN.

TITLEMolecularmappingofthemouseobmutation

JOURNALGenomics11（4）,1054-1062（1991）

PUBMED1686014

REFERENCE10（bases1to3444）

AUTHORSGroot,P.H.,Scholte,H.R.andHulsmann,W.C.

TITLEFattyacidactivation:

specificity,localization,andfunction

JOURNALAdv.LipidRes.14,75-126（1976）

PUBMED3952

COMMENTREVIEWEDREFSEQ:

ThisrecordhasbeencuratedbyNCBIstaff.The

referencesequencewasderivedfromDA762132.1,U43653.1,

AC018635.10andBU752306.1.

ThissequenceisareferencestandardintheRefSeqGeneproject.

OnMar12,2008thissequenceversionreplacedgi:

4557714.

Summary:

Thisgeneencodesaproteinthatissecretedbywhite

adipocytes,andwhichplaysamajorroleintheregulationofbody

weight.Thisprotein,whichactsthroughtheleptinreceptor,

functionsaspartofasignalingpathwaythatcaninhibitfood

intakeand/orregulateenergyexpendituretomaintainconstancyof

theadiposemass.Thisproteinalsohasseveralendocrine

functions,andisinvolvedintheregulationofimmuneand

inflammatoryresponses,hematopoiesis,angiogenesisandwound

healing.Mutationsinthisgeneand/oritsregulatoryregionscause

severeobesity,andmorbidobesitywithhypogonadism.Thisgenehas

alsobeenlinkedtotype2diabetesmellitusdevelopment.[provided

byRefSeq,Jul2008].

SequenceNote:

ThisRefSeqrecordwascreatedfromtranscriptand

genomicsequencedatatomakethesequenceconsistentwiththe

referencegenomeassembly.Thegenomiccoordinatesusedforthe

transcriptrecordwerebasedontranscriptalignments.

PublicationNote:

ThisRefSeqrecordincludesasubsetofthe

publicationsthatareavailableforthisgene.PleaseseetheGene

recordtoaccessadditionalpublications.

##Evidence-Data-START##

Transcriptexoncombination:

:

U43653.1,BC060830.1[ECO:

0000332]

RNAseqintrons:

:

singlesamplesupportsallintrons

ERS025084,ERS025087[ECO:

0000348]

##Evidence-Data-END##

COMPLETENESS:

fulllength.

PRIMARYREFSEQ_SPANPRIMARY_IDENTIFIERPRIMARY_SPANCOMP

1-309DA762132.11-309

310-1807U43653.1309-1806

1808-3383AC018635.1034398-35973

3384-3444BU752306.11-61c

FEATURESLocation/Qualifiers

source1..3444

/organism="Homosapiens"

/mol_type="mRNA"

/db_xref="taxon:

9606"

/chromosome="7"

/map="7q31.3"

gene1..3444

/gene="LEP"

/gene_synonym="LEPD;OB;OBS"