微流控芯片之一优质PPT.pptx
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Credits:
MathiesLab,UC-BerkeleyQuakeLab,StanfordAgilent,Inc.,二十一世纪科学发展趋势,MiniaturizationAutomationIntegrationPortable,LabonaChip?
Microfluidics,Microfluidics:
TheuseofmicrofabricationtechniquesfromtheICindustrytofabricatechannels,chambers,reactors,andactivecomponentsonthesizescaleofthewidthofahumanhairorsmaller,Credit:
Dr.KarenCheung,UBCECE,Whyusemicrofluidics?
Credit:
Dr.KarenCheung,UBCECE1moleculein1mL=1.6x10-18M1moleculein1nL=1.6x10-15M1moleculein1pL=1.6x10-12M,SamplesavingsnLofenzyme,notmLFasteranalysescanheat,coolsmallvolumesquicklyIntegrationcombinelotsofstepsontoasingledeviceNovelphysicsdiffusion,surfacetension,andsurfaceeffectsdominateThiscanactuallyleadtofasterreactions!
Whyusemicrofluidics?
1-3min/12samples,Microchipelectrophoresis1-3minMulti-channelmicrochipelectrophoresis,Highvoltage,buffer,sample,capillary,10-30min,Capillaryelectrophoresisseparationdetection,buffer,1-3,Gelelectrophoresis,SomeinterestingLOCapplications,GeneticanalysisPortabledevicesforpathogendetectionHigh-throughputbiologyChemicalsynthesisCellImmunoassayanalysisMetaboliteanalysisDrugdeliverysystemsSinglemolecularEnzymeanalysis,CourtesyofDr.BorisStoeber,Geneticanalysismicrosystems,96-channelradialcapillaryarrayelectrophoresismicroplate,AnalChem.1999;
71(23):
5354-61,Foodpoisoning,BacterialPathogensWoundinfections,Biowarfare,Foodpoisoning:
PathogenicE.coli:
1in10,000inCanadaSalmonellaspp.:
ashighas1in50eggs,Woundinfections:
Antibiotic-resistantS.aureus:
ashighas50%LungDisease:
Pseudomonasspp.MycobacteriumtuberculosisBiowarfare:
Anthrax,plague,smallpox,LungDisease,Dr.SharonPeacock,UniversityofOxford,MethodsforGeneticDetectionofPathogens,OLDWAY:
NEWWAY:
Developbacterialpathogendetectorsthatare:
Rapid(1hour)General(manydifferenttypesofpathogens)Sensitive(detectasinglecell)GeneticallySpecificField-portable,PCR-CEMicrosystemFabrication,PortableSystemforInfectiousDiseaseDetection,Systemmeasures8x10x12inches,Completefluorescencedetectionincluding488-nmsolid-statelaser,PMT,optics,anddetectionelectronicsHeaterandRTDelectronics4high-voltageCEpowersuppliesPDMSmicrovalvehardwareSimplePCinterface,Time(seconds),ratureC),ThermalCyclingCharacteristics,Time(seconds)5Xfasterthanconventionalthermalcyclers,Application:
Tuberculosis(TB)detection,DiseasecausedbyMycobacteriumtuberculosis9millionnewcasesofactiveTBeveryyearworldwide2millionTBdeathseveryyear450,000newcasesofmulti-drugresistantTB/yr10%ofTBcasesinSouthAfricaareXDRTBstrains,resistanttofirst-andsecond-lineantibioticsDoctorsWithoutBorders:
CurrentTBdetectionmethods,Fixationandstainingwithacid-faststains(Ziehl-Neelsenorauraminestains)Opticalorfluorescencemicroscopeexamination(10,000organisms/mL)Cultureinbiosafetylevel3containmentfacilityInselectcases,moleculardetectionisusedtoverifydiagnosisThatTBdestroysmillionsoflivesaroundtheworldeveryyearshowsthatthecurrentapproachisjustnotworkingThetoolswehavetotreatanddiagnoseTBarewoefullyinadequateandoutdated,andwerenotseeingthenecessaryurgencytotacklethedisease.Dr.TidovonSchoen-Angerer,Director,DWBsCampaignfortheAccesstoEssentialMedicines,TheNot-so-DistantFuture,2008PDA2308?
Paramount,384根通道扫描检测,Emrich,C.A.,etal.,AnalyticalChemistry,2002.74(19):
p.5076-5083.,10根通道CCD同时检测,Dang,F.Q.,etal.,AnalyticalChemistry,2005.77(7):
p.2140-2146.,目前最高通量微流控芯片平台,Aborn,J.H.,etal.,LabonaChip,2005.5(6):
p.669-674,门控进样多通道检测,Xu,X.M.,etal.,AnalyticalChemistry,2002.74(21):
p.5517-5522,高通量均相免疫分析,Bromberg,A.andR.A.Mathies,Electrophoresis,2004.25(12):
p.1895-1900,Microbes,cellsandsmallorganismscomeinawidearrayofshapesandsizesYeast,BloodCells,cryptosporidium,Lab-on-a-chipDevicesforCellSeparationandIdentification,MicrofluidicorLab-on-a-chipDevicescanbeusedtoseparatedifferentcelltypes,Variousbloodcellseparationshavebeendemonstrated:
J.A.Davis,D.W.Inglis,K.M.Morton,D.A.Lawrence,L.R.Huang,S.Y.Chou,J.C.Sturm,andR.H.Austin,Deterministichydrodynamics:
takingbloodapart,Proc.Nat.Acad.Sci.(USA),vol.103,pp.14779-14784,2006.,D.W.Inglis,J.A.Davis,T.J.Zieziulewicz,D.A.Lawrence,R.H.Austin,andJ.C.Sturm,Determiningbloodcellsizeusingmicrouidichydrodynamics,J.Immunol.Meth.,vol.329,pp.151-156,2008.D.W.Inglis,K.J.Morton,J.A.Davis,T.J.Zieziulewicz,D.A.Lawrence,R.H.Austin,andJ.C.Sturm,Microfluidicdeviceforlabel-freemeasurementofplateletactivation,SubmittedtoLabonaChip,Jan08.Butnoworkyetwithmicroorganisms!
Thisprojectwillleadtopublicationswhichdemonstratetheseparationofmicroorganismsonportableinexpensivepolymermicrofluidicdevices.,Suchdevicesmayhaveapplicationsinearlydetectionofhumandiseasessuchassepsisandvariouspar