美国药典USP32测试溶液配制.docx

美国药典USP32测试溶液配制.docx

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美国药典USP32测试溶液配制

TESTSOLUTIONS（TS）

Certainofthefollowingtestsolutionsareintendedforuseasacid-baseindicatorsinvolumetricanalyses.Suchsolutionsshouldbesoadjustedthatwhen0.15mLoftheindicatorsolutionisaddedto25mLofcarbondioxide-freewater,0.25mLof0.02Nacidoralkali,respectively,willproducethecharacteristiccolorchange.SimilarsolutionsareintendedforuseinpHmeasurement.Wherenospecialdirectionsfortheirpreparationaregiven,thesamesolutionissuitableforbothpurposes.

Whereitisdirectedthatavolumetricsolutionbeusedasthetestsolution,standardizationofthesolutionusedasTSisnotrequired.

Ingeneral,thedirectivetoprepareasolution“fresh”indicatesthatthesolutionisoflimitedstabilityandmustbepreparedonthedayofuse.

ForthepreparationofTestSolutions,usereagentsofthequalitydescribedunderReagents.

AcetaldehydeTS—Mix4mLofacetaldehyde,3mLofalcohol,and1mLofwater.Preparethissolutionfresh.

AcetateBufferTS—Dissolve320gofammoniumacetatein500mLofwater,add5mLofglacialaceticacid,dilutewithwaterto1000.0mL,andmix.ThissolutionhasapHbetween5.9and6.0.

AceticAcid,Glacial,TS—DeterminethewatercontentofaspecimenofglacialaceticacidbytheTitrimetricMethod（seeWaterDetermination921）.Iftheacidcontainsmorethan0.05%ofwater,addafewmLofaceticanhydride,mix,allowtostandovernight,andagaindeterminethewatercontent.Iftheacidcontainslessthan0.02%ofwater,addsufficientwatertomakethefinalconcentrationbetween0.02%and0.05%,mix,allowtostandovernight,andagaindeterminethewatercontent.Repeattheadjustmentwithaceticanhydrideorwater,asnecessary,untiltheresultingsolutionshowsawatercontentbetween0.02%and0.05%.

AceticAcid,Strong,TS—Add300.0mLofglacialaceticacid,anddilutewithwaterto1000mL.Thissolutioncontainsabout30%（v/v）ofCH3COOHandhasaconcentrationofabout5N.

AceticAcid–AmmoniumAcetateBufferTS—Dissolve77.1gofammoniumacetateinwater,add57mLofglacialaceticacid,anddilutewithwaterto1000mL.

Acetone,Buffered,TS—Dissolve8.15gofsodiumacetateand42gofsodiumchlorideinabout100mLofwater,andadd68mLof0.1Nhydrochloricacidand150mLofacetone.Mix,anddilutewithwaterto500mL.

AcidFerricChlorideTS—Mix60mLofglacialaceticacidwith5mLofsulfuricacid,add1mLofferricchlorideTS,mix,andcool.

AcidFerrousSulfateTS—SeeFerrousSulfate,Acid,TS.

AcidStannousChlorideTS—SeeStannousChloride,Acid,TS.

AcidStannousChlorideTS,Stronger—SeeStannousChloride,Acid,TS.

AlbumenTS—Carefullyseparatethewhitefromtheyolkofastrictlyfreshhen'segg.Shakethewhitewith100mLofwateruntilmixedandallbutthechalazahasundergonesolution;thenfilter.Preparethesolutionfresh.

Alcohol–PhenolTS—Dissolve780mgofphenolinalcoholtomake100mL.

AlcoholicAmmoniaTS—SeeAmmoniaTS,Alcoholic.

AlcoholicMercuricBromideTS—SeeMercuricBromideTS,Alcoholic.

AlcoholicPotassiumHydroxideTS—SeePotassiumHydroxideTS,Alcoholic.

AlkalineCupricCitrateTS—SeeCupricCitrateTS,Alkaline.

AlkalineCupricCitrateTS2—SeeCupricCitrateTS2,Alkaline.

AlkalineCupricIodideTS—SeeCupricIodideTS,Alkaline.

AlkalineCupricTartrateTS（Fehling'sSolution）—SeeCupricTartrateTS,Alkaline.

AlkalineMercuric–PotassiumIodideTS—SeeMercuric–PotassiumIodideTS,Alkaline.

AlkalinePicrateTS—SeePicrateTS,Alkaline.

AlkalineSodiumHydrosulfiteTS—SeeSodiumHydrosulfiteTS,Alkaline.

AmaranthTS—Dissolve20mgofamaranthin10mLofwater.

AminonaphtholsulfonicAcidTS—Accuratelyweigh5gofsodiumsulfite,94.3gofsodiumbisulfite,and700mgof1,2,4-aminonaphtholsulfonicacid,andmix.PrepareaminonaphtholsulfonicacidTSfreshonthedayofusebydissolving1.5gofthedrymixturein10mLofwater.

Ammonia–AmmoniumChlorideBufferTS—Dissolve67.5gofammoniumchlorideinwater,add570mLofammoniumhydroxide,anddilutewithwaterto1000mL.

Ammonia–CyanideTS—Dissolve2gofpotassiumcyanidein15mLofammoniumhydroxide,anddilutewithwaterto100mL.

AmmoniaTS—Itcontainsbetween9.5%and10.5%ofNH3.Preparebydiluting350mLofAmmoniaWater,Stronger（seeinthesection,Reagents）withwatertomake1000mL.

AmmoniaTS,Alcoholic—Asolutionofammoniagasinalcohol.Clear,colorlessliquidhavingastrongodorofammonia.Specificgravity:

about0.80.Itcontainsbetween9%and11%ofNH3.Storeitinalkali-resistantcontainers,inacoldplace.

AmmoniaTS,Stronger—UseAmmoniaWater,Stronger（seeinthesectionReagents）.

AmmoniacalPotassiumFerricyanideTS—Dissolve2gofpotassiumferricyanidein75mLofwater,add25mLofammoniumhydroxide,andmix.

AmmoniatedCupricOxideTS—SeeCupricOxide,Ammoniated,TS.

AmmoniumAcetateTS—Dissolve10gofammoniumacetateinwatertomake100mL.

AmmoniumCarbonateTS—Dissolve20gofammoniumcarbonateand20mLofammoniaTSinwatertomake100mL.

AmmoniumChlorideTS—Dissolve10.5gofammoniumchlorideinwatertomake100mL.

AmmoniumChloride–AmmoniumHydroxideTS—Mixequalvolumesofwaterandammoniumhydroxide,andsaturatewithammoniumchloride.

AmmoniumMolybdateTS—Dissolve6.5goffinelypowderedmolybdicacidinamixtureof14mLofwaterand14.5mLofammoniumhydroxide.Coolthesolution,andadditslowly,withstirring,toawell-cooledmixtureof32mLofnitricacidand40mLofwater.Allowtostandfor48hours,andfilterthroughafine-porosity,sintered-glasscrucible.Thissolutiondeterioratesuponstandingandisunsuitableforuseif,upontheadditionof2mLofdibasicsodiumphosphateTSto5mLofthesolution,anabundantyellowprecipitatedoesnotformatonceorafterslightwarming.Storeitinthedark.Ifaprecipitateformsduringstorage,useonlytheclearsupernatant.

AmmoniumOxalateTS—Dissolve3.5gofammoniumoxalateinwatertomake100mL.

AmmoniumPhosphate,Dibasic,TS（AmmoniumPhosphateTS）—Dissolve13gofdibasicammoniumphosphateinwatertomake100mL.

AmmoniumPolysulfideTS—Yellowliquid,madebysaturatingammoniumsulfideTSwithsulfur.

AmmoniumPyrrolidinedithiocarbamate,Saturated,TS—Addabout10gofammoniumpyrrolidinedithiocarbamatetoa1000-mLvolumetricflask,anddilutewithwatertovolume.

AmmoniumReineckateTS—Shakeabout500mgofammoniumreineckatewith20mLofwaterfrequentlyduring1hour,andfilter.Usewithin2days.

AmmoniumSulfideTS—SaturateammoniaTSwithhydrogensulfidebybubblinghydrogensulfidegasthroughthesolutionfor1minute.Thissolutionmustbefreshlyprepared.ThesolutionisnotrenderedturbideitherbymagnesiumsulfateTSorbycalciumchlorideTS（carbonate）.Thissolutionisunstableforuseifanabundantprecipitateofsulfurispresent.

Residueonignition:

notmorethan0.05%.

AmmoniumThiocyanateTS—Dissolve8gofammoniumthiocyanateinwatertomake100mL.

AmmoniumVanadateTS—Dissolve2.5gofammoniumvanadatein500mLofboilingwater,cool,andadd20mLofnitricacid.Mix,cool,andaddwatertomake1L.Storeinpolyethylenecontainers.

AnthroneTS—Within12hoursofuse,rapidlydissolve35mgofanthroneinahotmixtureof35mLofwaterand65mLofsulfuricacid.Immediatelycoolinanicebathtoroomtemperature,andfilterthroughglasswool.Allowthesolutiontostandatroomtemperaturefor30minutesbeforeuse.

AntimonyTrichlorideTS—Dissolve20gofantimonytrichlorideinchloroformtomake100mL.Filterifnecessary.

BariumChlorideTS—Dissolve12gofbariumchlorideinwatertomake100mL.

BariumHydroxideTS—Asaturatedsolutionofbariumhydroxideinrecentlyboiledwater.Preparethesolutionfresh.

BariumNitrateTS—Dissolve6.5gofbariumnitrateinwatertomake100mL.

BetanaphtholTS—See2-NaphtholTS.

BiuretReagentTS—Dissolve1.5gofcupricsulfateand6.0gofpotassiumsodiumtartratein500mLofwaterina1000-mLvolumetricflask.Add300mLofcarbonate-freesodiumhydroxidesolution（1in10）,dilutewithcarbonate-freesodiumhydroxidesolution（1in10）to1000mL,andmix.

BlueTetrazoliumTS—Dissolve500mgofbluetetrazoliuminalcoholtomake100mL.

BrilliantBlueGTS—Transfer25mgofbrilliantblueGtoa100-mLvolumetricflask,add12.5mLofalcoholand25mLofphosphoricacid,dilutewithwatertovolume,andmix.

BromineTS（BromineWater）—Asaturatedsolutionofbromine,preparedbyagitating2to3mLofbrominewith100mLofcoldwaterinaglass-stopperedbottle,thestopperofwhichshouldbelubricatedwithpetrolatum.Storeitinacoldplace,protectedfromlight.

Bromine–SodiumAcetateTS—Dissolve100gofsodiumacetatein1000mLofglacialaceticacid,add50mLofbromine,andmix.

p-BromoanilineTS—Add8gofp-bromoanilinetoamixtureof380mLofthiourea-saturatedglacialaceticacid,10mLofsodiumchloridesolution（1in5）,5mLofoxalicacidsolution（1in20）,and5mLofdibasicsodiumphosphatesolution（1in10）inalow-actinicglassbottle.Mix,andallowtostandovernightbeforeusing.Protectfromlight,andusewithin7days.

BromocresolBlueTS—UseBromocresolGreenTS.

BromocresolGreenTS—Dissolve50mgofbromocresolgreenin100mLofalcohol,andfilterifnecessary.

BromocresolGreen–MethylRedTS—Dissolve0.15gofbromocresolgreenand0.1gofmethylredin180mLofalcohol,anddilutewithwaterto200mL.

BromocresolPurpleTS—Dissolve250mgofbromocresolpurplein20mLof