Affymetrix生物芯片解决方案概述.docx

Affymetrix生物芯片解决方案概述.docx

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Affymetrix生物芯片解决方案概述

Affymetrix生物芯片解决方案概述

   Affymetrix公司作为全球销量第一的基因芯片厂家，以其完备的芯片设计，稳定可靠的分析结果和强大的生物信息学分析能力，帮助研究人员在最短的时间内获得大量可靠的结果，为后续研究提供重要的线索和帮助。

Affymetrix公司目前已经在纳斯达克上市，在基因芯片领域中成为行业标准。

   Affymetrix公司的巨大优势在于为客户提供“完整的基因芯片解决方案”，即提供全套的基因芯片相关产品。

包括：

1.性能优异、种类齐全的各类研究应用系列芯片产品；2.Affymetrix基因芯片相关试剂和试剂盒；3.基因芯片杂交、洗涤、扫描检测仪器系统及相关分析软件工具；4.基因芯片相关技术手册及使用指南等。

   独特的原位光刻技术；独特的PM-MM探针设计；严密的质控步骤；种类齐全，应用广泛的基因芯片；强大的配套分析软件，强大的网上注释及分析工具；还有发表的研究论文和项目合作及技术培训，想要了解Affymetrix芯片最具特色的技术特点，详细资料请点击进入......Affymetrix生物芯片解决方案概述

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胚胎及成体干细胞表达谱分析   SCIENCEVOL3022003

Commenton"'Stemness':

TranscriptionalProfilingofEmbryonicandAdultStemCells"and"AStemCellMolecularSignature"（I） 

Abstract

Ramalho-Santosetal.

（1）andIvanovaetal.

（2）,comparingthesamethree"stemcells"—embryonicstemcells（ESCs）;neuralstemcells（NSCs）,referredtoasneuralprogenitor/stemcells（NPCs）inthepresentstudy;andhematopoieticstemcells（HSCs）—withtheirdifferentiatedcounterparts,eachidentifiedalistofcommonlyexpressed"stemness"genes,proposedtobeimportantforconferringthefunctionalcharacteristicsofstemcells.Theabilitytocaptureexpressionprofilesofcellsusingmicroarraysoffersthepossibilityofdefiningastemcellbyitsconstellationofactivegenes.Anintriguingquestion,however,iswhetherthefunctionalcommonalities（self-renewalandpluripotency）（3）amongstemcellscanbedefinedatthegeneticlevel.Doallstemcellsexpressasimilarsetof"stemness"genesnecessaryfortheiruniqueproperties,ordodifferentstemcellsexpressdifferentsetsofgenesthatconferstemness?

拟南芥基因组插入突变表达谱分析   SCIENCEVOL3012003

Genome-WideInsertionalMutagenesisofArabidopsisthaliana 

Abstract

Over225,000independentAgrobacteriumtransferredDNA（T-DNA）insertioneventsinthegenomeofthereferenceplantArabidopsisthalianahavebeencreatedthatrepresentnearsaturationofthegenespace.Thepreciselocationsweredeterminedformorethan88,000T-DNAinsertions,whichresultedintheidentificationofmutationsinmorethan21,700ofthe29,454predictedArabidopsisgenes.Genome-wideanalysisofthedistributionofintegrationeventsrevealedtheexistenceofalargeintegrationsitebiasatboththechromosomeandgenelevels.Insertionmutationswereidentifiedingenesthatareregulatedinresponsetotheplanthormoneethylene. 

寡核苷酸微阵列技术对结肠腺瘤、腺癌的基因表达谱分析   CancerResearch61（7）,3124-30,2001

Transcriptionalgeneexpressionprofilesofcolorectaladenoma,adenocarcinoma,andnormaltissueexaminedbyoligonucleotidearrays

Abstract

Usinganoligonucleotidearraycontainingsequencescomplementaryto3200full-lengthhumancDNAsand3400expressedsequencetags（GeneChip,Affymetrix）,mRNAexpressionpatternswereprobedin18colonadenocarcinomasand4adenomas.Pairednormaltissuewasavailableandanalyzedforeachofthetumors.Relativelyfewchangesintranscriptexpressionareassociatedwithcoloncancer.Nineteentranscripts（0.48%ofthosedetected）hadatleast4–10.5-foldhighermRNAexpressionincarcinomacomparedwithpairednormalsamples,whereas47transcripts（1.3%ofthosedetected）hadatleast4–38-foldorlowerexpressioninthetumortissuecomparedwiththenormalsamples.Someofthesedifferenceswereconfirmedbyreversetranscription-PCR.Manyofthesetranscriptswerealreadyknowntobeabnormallyexpressedinneoplastictissueingeneral,orcoloncancerinparticular,andseveralofthesedifferenceswerealsoobservedinpremalignantadenomasamples.Atwo-wayhierarchicalclusteringalgorithmsuccessfullydistinguishedadenomafromadenocarcinomaandnormaltissue,generatingaphylogenetictreethatappropriatelyrepresentedtheclinicalrelationshipbetweenthethreetissuetypesincludedintheanalysis.Thissupportstheconceptthatgenome-wideexpressionprofilingmaypermitamolecularclassificationofsolidtumors.

利用DNA微阵列监测药物代谢及毒理相关基因的表达   PhysiologicalGenomics5（4）,161-70,2001

MonitoringexpressionofgenesinvolvedindrugmetabolismandtoxicologyusingDNAmicroarrays.

Abstract

OligonucleotideDNAmicroarrayswereinvestigatedforutilityinmeasuringglobalexpressionprofilesofdrugmetabolismgenes.Thisstudywasperformedtoinvestigatethefeasibilityofusingmicroarraytechnologytominimizethelong,expensiveprocessoftestingdrugcandidatesforsafetyinanimals.Inanevaluationofhybridizationspecificity,microarraytechnologyfromAffymetrixdistinguishedgenesuptoathresholdof90%DNAidentity.OligonucleotidesrepresentinghumancytochromeP-450geneCYP3A5showedheterologoushybridizationtoCYP3A4andCYP3A7RNAs.ThesegenescouldbeclearlydistinguishedbyselectingasubsetofoligonucleotidesthathybridizedselectivelytoCYP3A5.Furthervalidationofthetechnologywasperformedbymeasuringgeneexpressionprofilesinliversofratstreatedwithvehicle,3-methylcholanthrene（3MC）,phenobarbital,dexamethasone,orclofibrateandbyconfirmingdataforsixgenesusingquantitativeRT-PCR.Responsesofdrugmetabolismgenes,includingCYPs,epoxidehydrolases（EHs）,UDP-glucuronosyltransferases（UGTs）,glutathionesulfotransferases（GSTs）,sulfotransferases（STs）,drugtransportergenes,andperoxisomalgenes,tothesewell-studiedcompoundsagreedwellwith,andextended,publishedobservations.Additionalgeneregulatoryresponseswerenotedthatcharacterizemetaboliceffectsorstressresponsestothesecompounds.Thusmicroarraytechnologycanprovideafacileoverviewofgeneexpressionresponsesrelevanttodrugmetabolismandtoxicology.

表达图谱分析揭示不同细胞遗传学背景下的急性粒细胞白血病的生物学本质的区别  1124–1129PNASJanuary30,2001vol.98no.3

Expressionprofilingrevealsfundamentalbiologicaldifferencesinacutemyeloidleukemiawithisolatedtrisomy8andnormalcytogenetics

Abstract

Acutemyeloidleukemia（AML）isaheterogeneousgroupofdiseases.Normalcytogenetics（CN）constitutesthesinglelargestgroup,whiletrisomy8（+8）asasoleabnormalityisthemostfrequenttrisomy.Howtrisomycontributestotumorigenesisisunknown.Weusedoligonucleotide-basedDNAmicroarraystostudyglobalgeneexpressioninAML+8patientswith+8asthesolechromosomalabnormalityandAML-CNpatients.CD34+cellspurifiedfromnormalbonemarrow（BM）werealsoanalyzedasarepresentativeheterogeneouspopulationofstemandprogenitorcells.ExpressionpatternsofAMLpatientswereclearlydistinctfromthoseofCD34+cellsofnormalindividuals.WeshowthatAML+8blastsoverexpressgenesonchromosome8,estimatedat32%onaverage,suggestinggene-dosageeffectsunderlyingAML+8.Systematicanalysisbycellularfunctionindicatedup-regulationofgenesinvolvedincelladhesioninbothgroupsofAMLcomparedwithCD34+blastsfromnormalindividuals.Perhapsmostinterestingly,apoptosis-regulatinggenesweresignificantlydown-regulatedinAML+8comparedwithAML-CN.WeconcludethattheclinicalandcytogeneticheterogeneityofAMLisduetofundamentalbiologicaldifferences. 

利用SNP微阵列进行小细胞肺癌的杂合性的丢失（LOH）分析  NatureBiotechnology18（9）,1001-5,2000

Loss-of-heterozygosityanalysisofsmall-celllungcarcinomasusingsingle-nucleotidepolymorphismarrays

Abstract

Humancancersarisebyacombinationofdiscretemutationsandchromosomalalterations.Lossofheterozygosity（LOH）ofchromosomalregionsbearingmutatedtumorsuppressorgenesisakeyeventintheevolutionofepithelialandmesenchymaltumors.GlobalpatternsofLOHcanbeunderstoodthroughallelotypingoftumorswithpolymorphicgeneticmarkers.Simplesequencelengthpolymorphisms（SSLPs,ormicrosatellites）arereliablegeneticmarkersforstudyingLOH,butonlyamodestnumberofSSLPsareusedinLOHstudiesbecausethegenotypingprocedureisrathertedious.Here,wereporttheuseofahighlyparallelapproachtogenotypelargenumbersofsingle-nucleotidepolymorphisms（SNPs）forLOH,inwhichsamplesaregenotypedfornearly1,500locibyperforming24polymerasechainreactions（PCR）,poolingtheresultingamplificationproductsandhybridizingthemixturetoahigh-densityoligonucleotidearray.WecharacterizetheresultsofLOHanalysesonhumansmall-celllungcancer（SCLC）andcontrolDNAsamplesbyhybridization.WeshowthatthepatternsofLOHareconsistentwiththoseobtainedbyanalysiswithbothSSLPsandcomparativegenomichybridization（CGH）,whereasamplificationsrarelyaredetectedbytheSNParray.TheresultsvalidatetheuseofSNParrayhybridizationfortumorstudies.

小肠内寄主与微生物之间共生关系的分子水平分析  Science291（5505）,881-4,2001

Molecularanalysisofcommensalhost-microbialrelationshipsintheintestine

Abstract

Humanbeingscontaincomplexsocietiesofindigenousmicrobes,yetlittleisknownabouthowresidentbacteriashapeourphysiology.Wecolonizedgerm-freemicewithBacteroidesthetaiotaomicron,aprominentcomponentofthenormalmouseandhumanintestinalmicroflora.GlobalintestinaltranscriptionalresponsestocolonizationwereobservedwithDNAmicroarrays,andthecellularoriginsofselectedresponseswereestablishedbylaser-capturemicrodissection.Theresultsrevealthatthiscommensalbacteriummodulatesexpressionofgenesinvolvedinseveralimportantintestinalfunctions,includingnutrientabsorption,mucosalbarrierfortification,xenobioticmetabolism,angiogenesis,andpostnatalintestinalmaturation.Thesefindingsprovideperspectivesabouttheessentialnatureoftheinteractionsbetweenresidentmicroorganismsandtheirhosts. 

生物时钟对拟南芥信号传导途径中相关基因的表达调控 Science290（5499）,2110-3,2000

OrchestratedtranscriptionofkeypathwaysinArabidopsisbythecircadianclock

Abstract

Likemostorganisms,plantshaveendogenousbiologicalclocksthatcoordinateinternaleventswiththeexternalenvironment.Weusedhigh