生物类英语论文.docx

生物类英语论文.docx

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生物类英语论文

Effectofdifferentcarbonsourceandnitrogensourceonthecoldresistanceofcitruscallusinduction

HuSiyuan

Abstract:

Citruscallusisidealmaterialforphysicalandchemicalmutagenesisandgenetictransformation,thecarbonsourceandnitrogensourceisanecessarycomponentintheinductionandsubcultureofcitruscallus,sotheexplorationofcarbon,nitrogensourcesisofgreatsignificancetotheeffectdegreeofcitruscallusinductionandeffectway.Thisstudychoosecoldresistanceofcitrusastestmaterial,throughculturevitrotissues,analysisoftheeffectsofcarbonsourceandconcentration,nitrogensourceandlevelontheinfluenceofcitruscallusinduction,ToprovidethebasisforthestudyoftheoccurrenceandthemechanismofcoldresistanceofCitrusgeneticimprovement,somaticembryos.

Keywords:

carbonsource;nitrogensource;citrus;callus

Introduction

CitrusRutaceaeCitrus,smallevergreentreeorshrub,aboutthreemetershigh,weakbranches,usuallywiththorns.Leavesarealternate,leathery,lanceolatetoovate-lanceolate,5.5-8cmlong,2.9-4cmwide,apexacuminate,basecuneate,entireorwithfinebluntteeth,petioleslender,wingedobvious.Flowerssmall,yellow-white,solitaryorclusteredinleafaxils,sepals5;petals5;stamens18-24,filaments3-5piecesoftenconnate,ovary9-15rooms.Citrusfruitflatspherical,5-7cmindiameter,orange-yelloworreddishyellow,peelloosefleshmeateasilyseparated.

Chenggucitruscultivationhasalonghistorydatingbackmorethan2,000yearsofhistory.ChengguCountyislocatedinSouthernShaanxi,CentralHanzhongBasin,northsubtropicaltotemperatetransitionregion,northofQinlingmountainblockingtheinvasionofcoldnorthwest,southwindsBashansowarmaircannotdrivestraight.InversioneffectQinlingbeltandfoothillsslopingwall,formingauniquemicro-climateregions,fewerwintercoldandsummerheatless.First,here'scitrushightemperaturehotwinddoesnotharmflowering,fruitsettingrate;Secondcoolearlyautumn,temperature,andisconducivetofruitcolor,ripe,citrusfruitsurfacehereisgorgeous,highsolublesolidscontent,moderatelysweetandsour.

Studies[1-4]haveshownthatbychangingthecarbonsourcetoinducesomaticembryogenesis.Asmostofthecitruscallushasnotregenerateembryoidbodyphenomena[5-7],whilethehigh-frequencyinductionofsomaticembryogenesisofcitruscellsforgenetictransformation,invitromutagenesis,invitroscreeningmutants,rapidpropagation,etc.workhasspecialsignificance[8-9].Inthispaper,sucrose,glucose,solublestarch,threekindsofcarbonandnitrogensourcesammoniumnitrateonekindofgrowthandtheabilityofsomaticembryogenesisofcitruscalluswerestudiedatdifferentlevels,inordertoselecttheoptimalcultureconditionshardycitrusstudiesprovideevidence.

1.Purposeandsignificance

1.1Purpose

1.Analysistheinfluenceofcarbontypesandcarbonlevelsonhardyofcitrusinvitroculturedexplantsdedifferentiation;

2.Explorethenitrogensourceforcitruscallusinductioneffectmannerandextentofeffect;

3.Screeningsuitablecarbon,nitrogenconditionsofcitrusdedifferentiationinvitrocallusinduction.

1.2Significance

CitrusCalliisanidealmaterialforthephysicalandchemicalmutagenesisandgenetictransformation,andcarbonandnitrogenisanecessaryingredientincitruscallusinductionandsubculture,thereforeitisimportanttoexploretheextentandtheeffectofthewayoncitruscallusinductionofcarbonsource,nitrogensource.

1.Explorethecarbonandnitrogensourcesforcitruscallusinductioneffect,especiallyontheinductionrateanddifferentiationspeedforin-depthstudyofcarbonandnitrogeninthemechanismoftissueculturecitrusfromthebody,andtoprovidesomedatatosupport;

2.Hanzhongcitrusvarietiesforthestudymaterial,trytofilteroutthebestofitscallusinductionofcarbonrequired,thetypeandcontentofnitrogenfortheproductionofvirus-freecitrusseedlingsthroughcallusidealwaytoproviderelativelybasiccultureconditions.

2.Materialsandmethods

2.1Materials

Seedsofthetestmaterialexplantsofthesamesize,healthyfullChengguhardycitrusvarieties.

2.2Preparation

Instrument

HSserieshorizontalflowbench;thedish;verticalcircularchamberpressuresteamsterilizer;portableelectricpressuresteamsterilizerelectronicbalance;electricdistilledwater.

Auxiliarymaterials

<1>Inaplannedwayofcleaningtheglassware,andinnaturalconditions