限制性酶的酶切位点综述.docx

限制性酶的酶切位点综述.docx

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限制性酶的酶切位点综述

各种限制性酶的酶切位点

EnzymeNameSequence

AatIAGG!

CCT

AatIIGACGT!

C

AccIGT!

MKAC

AccIICG!

CG

AccIIIT!

CCGGA

AcyIGR!

CGYC

AflIG!

GWCC

AflIIC!

TTAAG

AflIIIA!

CRYGT

AhaICC!

SGG

AhaIIGR!

CGYC

AhaIIITTT!

AAA

AluIAG!

CT

AlwIGGATCNNNN!

AlwI!

NNNNNGATCC

AlwNICAGNNN!

CTG

AocICC!

TNAGG

AocIIGDGCH!

C

AosITGC!

GCA

AosIIGR!

CGYC

ApaIGGGCC!

C

ApaLIG!

TGCAC

ApyICC!

WGG

AquIC!

YCGRG

AseIAT!

TAAT

AspIGAANN!

NNTTC

AspIG!

GTACC

AspIGACN!

NNGTC

AsuIG!

GNCC

AsuIITT!

CGAA

AvaIC!

YCGRG

AvaIIG!

GWCC

AvaIIIATGCA!

T

AvrI!

CYCGRG

AvrIIC!

CTAGG

AxyICC!

TNAGG

BalITGG!

CCA

BamHIG!

GATCC

BanIG!

GYRCC

BanIIGRGCY!

C

BanIIIAT!

CGAT

BbeIGGCGC!

C

BbiII/AcyIGR!

CGYC

BbvIGCAGCNNNNNNNN!

BbvI!

NNNNNNNNNNNNGCTGC

BbvIIGAAGACNN!

BbvII!

NNNNNNGTCTTC

BcefIACGGCNNNNNNNNNNNN!

BcefI!

NNNNNNNNNNNNNGCCGT

BclIT!

GATCA

BcnICC!

SGG

BglIGCCNNNN!

NGGC

BglIIA!

GATCT

BinI!

NNNNNGATCC

BinIGGATCNNNN!

BsePI!

GCGCGC

BsmAI!

GTCTC

BsmAI!

GAGAC

BsmIGAATGCN!

BsmIG!

CATTC

BspIGDGCH!

C

BspHIT!

CATGA

BspMIACCTGCNNNN!

BspMI!

NNNNNNNNGCAGGT

BspMIIT!

CCGGA

BsrIACTGGN!

BsrIC!

CAGT

BssHIIG!

CGCGC

BstBITT!

CGAA

BstEIIG!

GTNACC

BstIG!

GATCC

BstNICC!

WGG

BstPIG!

GTNACC

BstUICG!

CG

BstXICCANNNNN!

NTGG

BstYIR!

GATCY

BsuICC!

TNAGG

CcrIC!

TCGAG

CfoIGCG!

C

CfrIR!

CCGGY

CfrIG!

GNCC

CfrIY!

GGCCR

ClaIAT!

CGAT

CviJIRG!

CY

CvnICC!

TNAGG

DdeIC!

TNAG

DpnIGA!

TC

DraITTT!

AAA

DraIIRG!

GNCCY

DraIIICACNNN!

GTG

DsaIC!

CRYGG

EaeIY!

GGCCR

EagIC!

GGCCG

EarI!

CTCTTC

EarI!

GAAGAG

EclXIC!

GGCCG

EcoITAC!

GTA

EcoIGGTCTCN!

EcoI!

NNNNNGAGACC

EcoIG!

GWCC

EcoIIIAGC!

GCT

EcoIC!

GGCCG

EcoI!

CTGAAG

EcoI!

CTTCAG

EcoICC!

TNAGG

EcoNICCTNN!

NNNAGG

EcoOIRG!

GNCCY

EcoRIG!

AATTC

EcoRII!

CCWGG

EcoRVGAT!

ATC

EcoTIC!

CWWGG

EcoTIATGCA!

T

EcoTIGRGCY!

C

EheIGGC!

GCC

EspIGC!

TNAGC

FinI!

GTCCC

FinI!

GGGAC

FnuHIGC!

NGC

FokIGGATGNNNNNNNNN!

FokI!

NNNNNNNNNNNNNCATCC

FspITGC!

GCA

GdiII!

NNNNNYGGCCG

GdiIICGGCCRN!

GsuI!

CTCCAG

GsuI!

CTGGAG

HaeIWGG!

CCW

HaeIIRGCGC!

Y

HaeIIIGG!

CC

HapIIC!

CGG

HgaIGACGCNNNNN!

HgaI!

NNNNNNNNNNGCGTC

HgiAIGWGCW!

C

HgiEII!

ACCNNNNNNGGT

HhaIGCG!

C

HinIGR!

CGYC

HinPIG!

CGC

HincIIGTY!

RAC

HindIIIA!

AGCTT

HinfIG!

ANTC

HpaIGTT!

AAC

HpaIIC!

CGG

HphIGGTGANNNNNNNN!

HphI!

NNNNNNNTCACC

KpnIGGTAC!

C

KspICTCTTCN!

KspI!

NNNNGAAGAG

MaeIC!

TAG

MaeIIA!

CGT

MaeIII!

GTNAC

MboI!

GATC

MboIIGAAGANNNNNNNN!

MboII!

NNNNNNNTCTTC

MfeI!

CAATTG

MflIR!

GATCY

MluIA!

CGCGT

MmeI!

TCCRAC

MmeI!

GTYGGA

MnlICCTCNNNNNNN!

MnlI!

NNNNNNNGAGG

MroIT!

CCGGA

MseIT!

TAA

MspIC!

CGG

MstITGC!

GCA

MstIICC!

TNAGG

MvaICC!

WGG

NaeIGCC!

GGC

NarIGG!

CGCC

NciICC!

SGG

NcoIC!

CATGG

NdeICA!

TATG

NdeII!

GATC

NheIG!

CTAGC

NlaIIICATG!

NlaIVGGN!

NCC

NotIGC!

GGCCGC

NruITCG!

CGA

NsiIATGCA!

T

Nsp（）IRCATG!

Y

Nsp（）VTT!

CGAA

NspBIICMG!

CKG

NspIIGDGCH!

C

NspIIIC!

YCGRG

NspIVG!

GNCC

NunIIGG!

CGCC

PaeRC!

TCGAG

PalIGG!

CC

PflMICCANNNN!

NTGG

PleIGAGTCNNNN!

PleI!

NNNNNGACTC

PmaCICAC!

GTG

PpuMIRG!

GWCCY

PstICTGCA!

G

PvuICGAT!

CG

PvuIICAG!

CTG

RsaIGT!

AC

RsrIG!

AATTC

RsrIICG!

GWCCG

SacIGAGCT!

C

SacIICCGC!

GG

SalIG!

TCGAC

SauAI!

GATC

SauIG!

GNCC

SauICC!

TNAGG

ScaIAGT!

ACT

ScrFICC!

NGG

SduIGDGCH!

C

SecIC!

CNNGG

SexI!

CTCGAG

SfaNIGCATCNNNNN!

SfaNI!

NNNNNNNNNGATGC

SfiIGGCCNNNN!

NGGCC

SinIG!

GWCC

SmaICCC!

GGG

SnaBITAC!

GTA

SnaI!

GTATAC

SpeIA!

CTAGT

SphIGCATG!

C

SplIC!

GTACG

SspIAAT!

ATT

SstIGAGCT!

C

SstIICCGC!

GG

SstIII!

ACGT

StuIAGG!

CCT

StyIC!

CWWGG

StySJI!

GAGNNNNNNGTRC

StySJI!

GYACNNNNNNCTC

TaqIT!

CGA

TaqIIGACCGANNNNNNNNNNN!

TaqII!

NNNNNNNNNTCGGTC

TaqIICACCCANNNNNNNNNNN!

TaqII!

NNNNNNNNNTGGGTG

ThaICG!

CG

TspI!

GTSAC

TspEI!

AATT

TthIGACN!

NNGTC

TthIICAARCANNNNNNNNNNN!

TthII!

NNNNNNNNNTGYTTG

TthHBIT!

CGA

VspIAT!

TAAT

XbaIT!

CTAGA

XcyIC!

CCGGG

XhoIC!

TCGAG

XhoIIR!

GATCY

XmaIC!

CCGGG

XmaIIIC!

GGCCG

XmnIGAANN!

NNTTC

XorIICGAT!

CG