Expression on different time Intracerebroventricular subventricular zone Musashi1 after birth毕业论.docx

Expression on different time Intracerebroventricular subventricular zone Musashi1 after birth毕业论.docx

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ExpressionondifferenttimeIntracerebroventricularsubventricularzoneMusashi1afterbirth毕业论

ExpressionondifferenttimeIntracerebroventricularsubventricularzoneMusashi1afterbirth

The:

MaQiaoyingofTie-JunXuWangHemingWangMeishen

[Keywords:

]RNAbindingprotein;Musashi1;neuralstemcells;subventricularzone;rats

Abstract:

ObjectiveToobservetheneuron-specificRNAbindingproteinMusashi1（Msi1indifferentdevelopmentaltimeafterbirthlateralventriclesubventricularzone（withintheSVZexpressionchange.MethodsMaleSDrats,dividedintosevendays,twoweeks,threeweeksJanuary,February,adult（6monthsofageandolder（18monthsold）group,n=5,35,paraformaldehydefixed,frozencoronalsections,immunohistochemistry（ABCstaining,lightmicroscopecamera,cellcountresultsseeninratsofvariousagegroupsperiventricularSVZtheMsi1immuneresponsepositivecells,smallroundcellbody,noprotrusionsor1to2shortprotruding,unevendistributionofdensity;increasingageMsi1positivecells,SVZgraduallyreduced,thereisstillacertainamountofMsi1-positivecellsintheagedrats.conclusionsIntracerebroventricularSVZmemoryMsi1positivecellsandagesignificantlyreduced,thesecellsmaybeneuralstemcells.

Keywords:

:

RNA-bindingprotein;Musashi1;neuralstemcells;subventricularzone;ratsAbstract:

ObjectiveToobservetheexpressionofneuralRNA-bindingproteinsMusashi1（Msi1inthesubventricularzone（SVZ）oflateralventricles（LVatdifferentpostnataldevelopingtimes.MethodsHealthymaleSDratsofdifferentagegroupswererecruited（1-,2-and3-weekgroups,1-and2-monthgroups,6-monthgrowngroup,and18-monthagedgroup;n=5each.Immunohistochemistrywasperformedonthecoronalsectionsofbrainoftheratsperfusedwithparaformaldehyde.ResultsTheexpressionofMsi1wasobservedintheSVZofLVinallgroups.Msi1-positivecellsweresmallandroundinshape,showingnoor1~2shortprocessesonthesurface.TheMusashi1-positivecellswereunevenlydis-tributed.Thenumberofthemwasreducedgraduallywithage.ConclusionManyMusashi1-positivecellsarepresentintheSVZofratthewholelife,withtheirnumberreducinggraduallywithage.Theymightplayasneuralstemcells.

Keywords:

:

RNA-bindingproteins;Musashi1;neuralstemcells;subventricularzone;ratMusashi1（Msi1isaneuralRNA-bindingprotein,mammalianMsi1homologoftheDrosophilatheMusashiproteinintheDrosophilaMusashiproteinnecessaryfeelingasymmetricdivisionofneuralprecursorcells[1-2].Experimentalproof,Msi1selectivelyexpressedinneuralstemcells,playakeyroleonthemaintenanceofstemcellstateanddifferentiation,andisconsideredtobeamarkerofmammalianneuralstemcells[3~6].Msi1mRNAinteractionwithnumbgenesnumbgeneexpressionfromthetranslationalleveladjustment,therebyregulatingtheactivitiesoftheNotchsignaling,ultimatelyaffectneuralstemcellself-renewalanddifferentiation[7]Msi1ismainlydistributedintheperinuclearcytoplasmicinthedevelopmentofthemammaliancentralnervoussystemstemcells,Msi1abundantexpressioninadultrodentsubventricularzone（thesubventricularzoneintheSVZproliferatingcellsstillMsi1expression[3-4].singlecellcultureconfirmedthatallnewbornneuralstemcellsweretestedMsi1immunepositivereactionintheproliferationofpluripotentneuralprogenitorcellsMsi1expression,butinthenewlygeneratedtherepostmitoticneuronsMsi1freeexpression.vivoexperimentsshowedthat,Msi1theexpressionaccompaniedwithneurogenesiscarriedoutanddown,toadultstageonlyaverylowlevel[8]Nevertheless,Msi1asaneuralRNAbindingproteinexpressionchangesinthedevelopmentalstageintheratSVZafterbirth,howhasnotbeenreported.

Therefore,theexperimentaluseofimmunohistochemicalmethods,detectionoftheMsi1thePOSTNATALdifferenttimeswithinthelateralventricleSVZexpressionandmorphologicaldatatoexplorethedevelopmentofthenervoussystem.

1Materialsandmethods1.1ExperimentalanimalsandgroupsofhealthymaleSD（Sprague-Dawleyrats,providedbytheExperimentalAnimalCenterofXuzhouMedicalCollege.Dividedintosevendays,twoweeks,threeweeks,January,February,adult（6monthsofageandolder（187groupsof5-month-old,atotalof35.

1.2ratswereperfusionfixedratswithpentobarbitalsodiumconventionalintraperitonealinjectionofanesthesia,thechestwasopened,andattentive-ascendingaorticcannulationperfusion.’sTakeaquickinfusionofsaline,then40g.L（4%paraformaldehydeperfusionfixedafterreperfusion,theratsweredecapitatedandexposedbrain,thestereotaxicinstrumentontheratbraincoronaldividedinto,afterparagraph3,thesamefixativefixed,4℃overnight,complexmovedintothe15%sucrosesolution,sinktothebottomandthenmovedtothe30