1、Expression on different time Intracerebroventricular subventricular zone Musashi1 after birth毕业论 Expression on different time Intracerebroventricular subventricular zone Musashi1 after birth The: Ma Qiaoying of Tie-Jun Xu Wang Heming Wang Meishen Keywords: RNA binding protein; Musashi1; neural stem
2、cells; subventricular zone; rats Abstract: Objective To observe the neuron-specific RNA binding protein Musashi1 (Msi1 in different developmental time after birth lateral ventricle subventricular zone (within the SVZ expression change. Methods Male SD rats, divided into seven days, two weeks, three
3、weeks January, February, adult (6 months of age and older (18 months old) group, n = 5, 35, paraformaldehyde fixed, frozen coronal sections, immunohistochemistry (ABC staining, light microscope camera, cell count results seen in rats of various age groups periventricular SVZ the Msi1 immune response
4、 positive cells, small round cell body, no protrusions or 1 to 2 short protruding, uneven distribution of density; increasing age Msi1 positive cells, SVZ gradually reduced, there is still a certain amount of Msi1-positive cells in the aged rats. conclusions Intracerebroventricular SVZ memory Msi1 p
5、ositive cells and age significantly reduced, these cells may be neural stem cells. Keywords: RNA-binding protein; Musashi1; neural stem cells; subventricular zone; rats Abstract: Objective To observe the expression of neural RNA-binding proteins Musashi1 (Msi1in the subventricular zone (SVZ) of late
6、ral ventricles (LVat different postnatal developing times.Methods Healthy male SD rats of different age groups were recruited (1 - ,2-and3 -week groups ,1-and2-month groups ,6-month grown group, and18-month aged group; n = 5each.Immunohistochemistry was performed on the coronal sections of brain of
7、the rats perfused with paraformaldehyde.Results The expression of Msi1was observed in the SVZ of LV in all groups.Msi1-positive cells were small and round in shape, showing no or1 2short processes on the surface.The Musashi1-positive cells were unevenly dis-tributed.The number of them was reduced gr
8、adually with age. Conclusion Many Musashi1-positive cells are present in the SVZ of rat the whole life, with their number reducing gradually with age.They might play as neural stem cells. Keywords: RNA-binding proteins; Musashi1; neural stem cells; subventricular zone; rat Musashi1 (Msi1 is a neural
9、 RNA-binding protein, mammalian Msi1 homolog of the Drosophila the Musashi protein in the Drosophila Musashi protein necessary feeling asymmetric division of neural precursor cells 1-2. Experimental proof , Msi1 selectively expressed in neural stem cells, play a key role on the maintenance of stem c
10、ell state and differentiation, and is considered to be a marker of mammalian neural stem cells 3 6. Msi1 mRNA interaction with numb genes numb gene expression from the translational level adjustment, thereby regulating the activities of the Notch signaling, ultimately affect neural stem cell self-re
11、newal and differentiation 7 Msi1 is mainly distributed in the perinuclear cytoplasmic in the development of the mammalian central nervous system stem cells , Msi1 abundant expression in adult rodent subventricular zone (the subventricular zone in the SVZ proliferating cells still Msi1 expression 3-4
12、. single cell culture confirmed that all newborn neural stem cells were tested Msi1 immune positive reaction in the proliferation of pluripotent neural progenitor cells Msi1 expression, but in the newly generated there postmitotic neurons Msi1 free expression. vivo experiments showed that, Msi1 the
13、expression accompanied with neurogenesis carried out and down, to adult stage only a very low level 8 Nevertheless, Msi1 as a neural RNA binding protein expression changes in the developmental stage in the rat SVZ after birth, how has not been reported. Therefore, the experimental use of immunohisto
14、chemical methods, detection of the Msi1 the POSTNATAL different times within the lateral ventricle SVZ expression and morphological data to explore the development of the nervous system. 1 Materials and methods 1.1 Experimental animals and groups of healthy male SD (Sprague-Dawley rats, provided by
15、the Experimental Animal Center of Xuzhou Medical College. Divided into seven days, two weeks, three weeks, January, February, adult (6 months of age and older (18 7 groups of 5-month-old, a total of 35. 1.2 rats were perfusion fixed rats with pentobarbital sodium conventional intraperitoneal injecti
16、on of anesthesia, the chest was opened, and attentive - ascending aortic cannulation perfusion.s Take a quick infusion of saline, then 40g.L (4% paraformaldehyde perfusion fixed after reperfusion, the rats were decapitated and exposed brain, the stereotaxic instrument on the rat brain coronal divided into, after paragraph 3, the same fixative fixed, 4 overnight, complex moved into the 15% sucrose solution , sink to the bottom and then moved to the 30
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