山东大学分子生物学相关资料.docx

山东大学分子生物学相关资料.docx

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山东大学分子生物学相关资料

SectionA-Cellsandmacromolecules

1．Theglycosylationofsecretedproteinstakesplaceinthe...

Amitochondria.

Bperoxisomes.

Cendoplasmicreticulum.

Dnucleus.

2．Whichofthefollowingisanexampleofanucleoprotein?

Akeratin.

Bchromatin.

Chistone.

Dproteoglycan.

3．Whichofthefollowingisnotapolysaccharide?

Achitin.

Bamylopectin.

Cglycosaminoglycan.

Dglycerol.

4.Transmembraneproteins

Ajointwolipidbilayerstogether.

Bhaveintra-andextracellulardomains.

Carecontainedcompletelywithinthemembrane.

Dareeasilyremovedfromthemembrane.

SectionB-Proteinstructure

1.Whichofthefollowingisaniminoacid?

Aproline.

Bhydroxylysine.

Ctryptophan.

Dhistidine.

2．Proteinfamilymembersindifferentspeciesthatcarryoutthesamebiochemicalrolearedescribedas...

Aparalogs.

Bstructuralanalogs.

Cheterologs.

Dorthologs.

3.Whichofthefollowingisnotaproteinsecondarystructure?

Aα-helix.

Btriplehelix.

Cdoublehelix.

Dß-pleatedsheet.

4．Inisoelectricfocusing,proteinsareseparated.

AinapHgradient.

Binasaltgradient.

Cinadensitygradient.

Dinatemperaturegradient.

5．Edmandegradationsequencespeptides...

AusingacDNAsequence.

Baccordingtotheirmasses.

CFromtheC-terminustotheN-terminus.

DfromtheN-terminustotheC-terminus.

SectionC-propertiesofnucleicacids

1．Thesequence5'-AGTCTGACT-3'inDNAisequivalenttowhichsequenceinRNA?

A5'-AGUCUGUGACU-3'

B5'-UGTCTGUTC-3'

C5'-UCAGUCUGA-3'

D5'-AGUCAGACU-3'

2.WhichofthefollowingcorrectlydescribesA-DNA?

Aaright-handedantiparalleldoublehelixwith10bp/turnandbaseslyingperpendiculartothehelixaxis.

Baleft-handedantiparalleldouble-helixwith12bp/turnformedfromalternatingpyrimidine-purinesequences.

Caright-handedantiparalleldoublehelixwith11bp/turnandbasestiltedwithrespecttothehelixaxis.

Daglobularstructureformedbyshortintramolecularhelicesformedinasingle-strandnucleicacid.

3.DenaturationofdoublestrandedDNAinvolves.

Apreakageintoshortdouble-strandedfragments.

Bseparationintosinglestrands.

ChydrolysisoftheDNAbackbone.

Dcleavageofthebasesfromthesugar-phosphatebackbone.

4.Whichhasthehighestabsorptionperunitmassatawavelengthof260nm?

Adouble-strandedDNA.

Bmononucleotides.

CRNA.

Dprotein.

5.TypeIDNAtopoisomeraes...

Achangelinkingnumberby士2

BrequireATP.

CbreakonestrandofaDNAdoublehelix.

Darethetargetofantibacterialdrugs.

SectionD-Prokaryoticandeukaryoticchromatinstructure

1．Whichofthefollowingiscommonto·bothE.coliandeukaryoticchromosomes?

AtheDNAiscircular.

BtheDNAispackagedintonucleosomes.

CtheDNAiscontainedinthenucleus.

DtheDNAisnegativelysupercoiled.

2．Acomplof166bpofDNAwiththehistoneoctamerplushistoneHIisknownasa...

Anucleosomecore.

Bsolenoid.

C30nmfiber.

Dchromatosome.

3．Inwhatregionoftheinterphasechromosomedoestranscriptiontakeplace?

Athetelomere.

Bthecentromere.

Ceuchromatin.

Dheterochromatin.

4．WhichstatementaboutCpGislandsandmethylationisnottrue?

ACpGislandsareparticularlyresistanttoDNaseI.

BCpGmethylationisresponsibleforthemutationofCpGtoTpGineukaryotes.

CCpGislandsoccuraroundthepromotersofactivegenes.

DCpGmethylationisassociatedwithinactivechromatin.

5．Whichofthefollowingisanexampleofhighly-repetitiveDNA?

AAluelement.

Bhistonegenecluster.

CDNAminisatellites.

DdispersedrepetitiveDNA.

SectionE-DNAreplication

1．Thenumberofrepliconsinatypicalmammaliancellis...

A40-200.

B400.

C1000-2000.

D50000-100000.

2.Inprokaryotes,thelaggingstrandprimersareremovedby...

A3'to5'exonuclease.

BDNAligase.

CDNApolymeraseI.

DDNApolymeraseIII.

3.TheessentialinitiatorproteinattheE.colioriginofreplicationis...

ADnaA.

BDnaB.

CDnaC.

DDnaE.

4.WhichphasewouldacellenterifitwasstarvedofmitogensbeforetheRpoint?

AG1.

BS.

CG2.

DG0.

5.Whichoneofthefollowingstatementsistrue?

AoncethecellhaspassedtheRpoint,celldivisionisinevitable.

BthephosphorylationofRbbyaG1cyclin-CDKcomplexisacriticalrequirementforentryintoSphase.

CphosphorylationofE2FbyaG1cyclin-CDKcomplexisacriticalrequirementforentryintoSphase.

DcyclinD1andINK4p16aretumorsuppressorproteins.

6.Ineukaryotes,euchromatinreplicatespredominantly...

AinearlyS-phase.

BinmidS-phase.

CinlateS-phase.

DinG2-phase.

7.Prokaryoticplasmidscanreplicateinyeastcellsiftheycontainaclonedyeast...

AORC.

BCDK.

CARS.

DRNA.

SectionF-DNAdamage,repairandrecombination

1.Pernucleotideincorporated,thespontaneousmutationfrequencyinE.coliis...

A1in106.

B1in108.

C1in109.

D1in1010.

2.TheactionofhydroxylradicalsonDNAgeneratesasignificantamountof...

Apyrimidinedimmers.

B8-oxoguanine.

CO6-methylguanine.

D7-hydroxymethylguanine.

3.Inmethyl-directedmismatchrepairinE.coli,thedaughterstrandcontainingthemismatchedbaseisnickedby...

AMutHendonuclease.

BUvrABCendonuclease.

CAPendonuclease.

D3'to5'exonuclease.

4.Illegitimaterecombinationisanothernamefor...

Asite-specificrecombination.

Btransposition.

Chomologousrecombination.

DtranslesionDNAsynthesis.

5.TheexcisionrepairofUV-inducedDNAdamageisdefectiveinindividualssufferingfrom...

Ahereditarynonpolyposiscoloncancer.

BCrohn'sdisease.

Cclassicalxerodermapigmentosum.

Dxerodermapigmentosumvariant.

SectionG-Genemanipulation

1.Thepresenceofaplasmidinabacterialcultureisusuallydeterminedby...

Ablue-whitescreening.

Bgrowthinthepresenceofanantibiotic.

Carestrictionenzymedigest.

Dagarosegelelectrophoresis.

2.Theenzymealkalinephosphatase...

Athetake-upofaplasmidintoabacterium.

Btheexpressionofageneinabacterium.

Cthetake-upofabacteriophageintoabacterium.

Dtheisolationofaplasmidfromabacterium.

3.Transformationis...

Athetake-upofaplasmidintoabacterium.

Btheexpressionofageneinabacterium.、

Cthetake-upofabacteriophageintoabacterium.

Dtheisolationofaplasmidfromabacterium.

4.T4DNAligase...

ArequiresATP.

Bjoinsdouble-strandedDNAfragmentswithanadjacent3'-phosphateand5'-OH.

CrequiresNADH.

Djoinssingle-strandedDNA.

5.Inagarosegelelectrophoresis...

ADNAmigratestowardsthenegativeelectrode.

Bsupercoiledplamidsmigrateslowerthantheirnickedcounterparts.

Clargermoleculesmigratefasterthansmallermolecules.

DethidiumbromidecanbeusedtovisualizetheDNA.

SectionH-Cloningvectors

1.Blue-whiteselectionisused...

Atotestforthepresenceofaplasmidinbacteria.

BtorevealtheidentityofaclonedDNAfragment.

Ctoexpresstheproductofaclonedgene.

Dtotestforthepresenceofaclonedinsertinaplasmid.

2.Amultiplecloningsite...

Acontainsmanycopiesofaclonedgene.

Ballowsflexibilityinthechoiceofrestrictionenzymesforcloning.

Callowsflexibilityinthechoiceoforganismforcloning.

Dcontainsmanycopiesofthesamerestrictionenzymesite.

3.InfectionofE.colibybacteriophageλisnormallydetectedby...

Aresistanceofthebacteriatoanantibiotic.

Bthegrowthofsinglebacterialcoloniesonanagarplate.

Ctheappearanceofareasoflysedbacteriaonanagarplate.

DrestrictiondigestofthebacterialDNA.

4.Whichvectorwouldbemostappropriateforcloninga150kbfragmentofDNA?

Aaplasmid.

Baλvector.

CaBAC.

DaYAC.

5.Whichvectorwouldyouchqosetoexpressaforeigngeneinaplant?

Aabaculovirusvector.

Baretroviralvector.

CaYepvector.

DaT-DNAvector.

SectionI-Genelibrariesandscreening

1.Whichtwoofthefollowingstatementsaboutgenomiclibrariesarefalse?

AgenomiclibrariesaremadefromcDNA.

Bgenomiclibrariesmustberepresentativeiftheyaretocontainallthegenesinanorganism.

CgenomiclibrariesmustcontainaminimumnumberofrecombinantsiftheyaretocontainallthegenesInanorgamsm.

DtheDNAmustbefragmentedtoanappropriatesizeforthevectorthatisused.

EgenomiclibrariesmadefromeukaryoticDNAusuallyuseplasmidvectors.

2.WhichstatementcorrectlydescribessequentialstepsincDNAcloning?

AreversetranscriptionofMrnasecondstrandsynthesiscDNAendmodificationligationtovector.

BmRNApreparationcDNAsynthesisusingreversetranscriptasesecondstrandsynthesisusingterminaltransferase,ligationtovector.

CmRNAsynthesisusingRNApolymerasereversetranscriptionofmRNA,secondstrandsynthesis,ligationtovector.

DdoublestrandedcDNAsynthesisrestrictionenzymedigestionadditionoflinkersligationtovector.

3.Whichoneofthefollowingisnotavalidmethodofscreeningalibrary?

Ahybridizationofcolony/plaque-liftedDNAusinganucleicacidprobe.

Busingantibodiesraisedagainsttheproteinofinteresttoscreenanexpressionlibrary.

Cscreeningpoolsofclonesfromanexpressionlibraryforbiologicalactivity.

Dhybridizationofcolony/plaque-liftedDNAusinganantibodyprobe.

SectionJ-AnalysisandusesofclonedDNA

1.AlinearDNAfragmentis（10