武汉大学至学年第一学期分子生物学期末考试试题Word下载.docx

武汉大学至学年第一学期分子生物学期末考试试题Word下载.docx

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DESCRIPTION（2pointseach）

Youranswershoulddescribewhateachitemisandhowitfunctionsinthecell.Diagrams,structureandsequenceinformationshouldbeincludedinyouranswer,asnecessary.

1. 

Yeastartificialchromosome

2. 

RNAinterference

3. 

Proteomics

4. 

Shine-Dalgarnosequence

5. 

Alternativesplicing

6. 

Ribozyme

7. 

r-dependenttermination

8. 

RNAediting

9. 

DNAlesions

10. 

Proteintargeting

PARTII:

MULTIPLECHOICES 

（1pointseach）

Selectthe 

onebestanswer 

foreachquestion.

1.Thecatalyticactivityforpeptidebondformation（thepeptidyltransferaseactivity）islocatedinthe:

1） 

RNAofthelargeribosomalsubunit.

2） 

leadersequenceofthemessengerRNA.

3） 

RNAofthesmallribosomalsubunit.

4） 

proteinsofthesmallribosomalsubunit.

5） 

proteinsofthelargeribosomalsubunit.

2.Bidirectionalandsemi-conservativearetwotermsthatreferto:

transcription.

translation.

replication.

alloftheabove.

noneoftheabove.

3.Thefactthatmostaminoacidsarespecifiedbymultiplecodonsisknownas:

the“wobble”phenomenon.

theuniversalityofthegeneticcode.

codonbias.

theanticodonhypothesis.

theredundancyofthegeneticcode.

4.RNApolymeraseIistheeukaryoticenzymeresponsiblefor:

transcriptionofribosomalRNA.

transcriptionoftransferRNAandothersmallRNAspecies.

transcriptionofmessengerRNA.

initiationofOkazakifragmentsynthesisinDNAreplication.

5.RestrictionenzymescancleaveDNAthatiseithersingle-strandedordouble-stranded,aslongasitcontainstheappropriaterecognitionsite.

1）True 

2）False

6.Informationaboutthesequenceofthecodingregionofageneisbestobtainedfrom:

aYACclone.

agenomicclone.

acDNAclone.

theprotein.

7.Achromatographymethodthatcanbeusedspecificallytopurifyproteinsbasedontheirchargeis:

gelfiltrationchromatography.

ion-exchangechromatography.

DNAaffinitychromatography.

antibodyaffinitychromatography.

8.AnonsensemutationisachangeintheDNAsequencethatresultsin:

asmalldeletionorinsertion.

anaminoacidchangeintheproteinencodedbythegene.

aprematurestopcodon.

9.Aproteincomplexinvolvedindegradationofproteinswithinthecellisknownasthe:

ubiquitin/proteasomesystem.

molecularchaperone.

chaperonin.

ribosome.

Krebs/TCAcycle.

10.___bindstotherepressorandturnonthetranscriptionofthestructuralgenesintheLacoperon.

cAMP

lactose

allolactose

CRP

11.WhichofthefollowingRNAspeciesisinvolvedindegradationofthemRNAcontainingcomplementarysequence

miRNA

siRNA

tRNA

5SRNA

U3snRNA

12.Thegenomesequencingprojectsareconfirmingthetheorythatgenomesizeisdirectlyproportionaltothenumberofgenescontainedwithinthatgenome.Inotherwords,agenomethatis10timesasbigwillcontainsapproximately10timesasmanyproteincodinggenes.

13.HeLacells,derivedfromahumancervicalcarcinoma,areabletopropagateindefinitelyincultureandarethereforeknownasa（n）:

tissueculture.

tumor.

transgeniccellline.

immortalizedcellline.

14. 

E.coli 

cellsaresmallerthanyeastcells.

15.WhichofthefollowingdomainsisnotaDNAbindingdomain

Proline-richdomains

Helix-turnhelixdomains

Zincfingerdomains

Basicdomains

16.Theaminoacyl-tRNAsynthetasesdistinguishbetweenabout40differentshapedtRNAmoleculesinthecells.

PARTIII:

SHORTQUESTIONS 

（8pointseach）

Howdobacterialreplicationstartandaccomplished.Remembertoincludetheproteins/enzymesandimportantDNAsequenceinvolvedinthisprocess.

Designexperimentsto 

clone 

ayeastgeneand 

express 

thisgeneinyeast.

Belowisthemultiplecloningsite（MCS）oftheplasmidvectorpUC18andtheN-terminalandC-terminalsequenceofproteinX.NotethattheMCSconstitutesapartoftheLacZopenreadingframe.Supposethatyouaregoingtoclonethe