骨科组织工程动物模型_精品文档PPT课件下载推荐.ppt

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ChineseNationalOrthopaedicMeeting-July4,2004ChineseNationalOrthopaedicMeeting-July4,2004AnimalModelsforOrthopaedicTissueEngineeringYuehueiH.An,MDYuehueiH.An,MDAssociateProfessorandDirectorAssociateProfessorandDirectorOrthopaedicResearchLaboratoryOrthopaedicResearchLaboratoryMedicalUniversityofSouthCarolinaMedicalUniversityofSouthCarolinaCharleston,SouthCarolinaCharleston,SouthCarolinaNote:

@#@AllillustrationsandimagesareoriginalNote:

@#@AllillustrationsandimagesareoriginalBicyclingBicyclingBeijing-Beijing-HainanHainanIslandIslandApril1998April1998Beijing-Beijing-HainanHainanIslandIslandBicycleTripBicycleTrip19881988TissueEngineeringCatoT.Laurencinetal（1999）:

@#@“Theapplicationofbiological,chemical,andengineeringprinciplestowardstherepair,restorationandregenerationoflivingtissueusingbiomaterials,cells,andfactorsaloneorincombination”The“3Rs”MainSubtitles:

@#@I.HowtoChooseAnimalModelsII.CommonlyUsedModelsIII.ProjectDesigningIV.MethodsofEvaluationResearchersshouldconsiderthefollowingissueswhenselectingaresearchanimal:

@#@EthicsEthicsAvailabilityAvailabilityHousingRequirementsHousingRequirementsEaseofHandlingEaseofHandlingCostsCostsDiseasesDiseasesBackgroundDataoftheAnimalBackgroundDataoftheAnimalI.HowToChooseAnimalsEthics/AnimalResearchBalance-Publicconcernsrabbits,pigs,andgoatsdogs,cats-Ratsrabbits,pigs,andgoatsdogs,cats（Rats,pigs,rabbits,orgoatsshouldbeconsidered（Rats,pigs,rabbits,orgoatsshouldbeconsideredfirst.Dogsandcatsareusedifitisessential.）first.Dogsandcatsareusedifitisessential.）-DirectlyapplicabletopatientsBasicscience-DirectlyapplicabletopatientsBasicscience-Lesspainandsufferingmorepain-Lesspainandsufferingmorepain-Lessnumberlargenumber-LessnumberlargenumberEthics“3Rs”In1959,RussellandBurch,twoEnglishscientists,In1959,RussellandBurch,twoEnglishscientists,introducedintroducedThePrinciplesofHumaneExperimentalThePrinciplesofHumaneExperimentalTechniqueTechnique.-“The3Rs”:

@#@.-“The3Rs”:

@#@Replacementofanimals,byofanimals,byinvitroinvitromethodsmethodsorormoreprimitiveanimalsmoreprimitiveanimalsReductionofthenumberofanimalsofthenumberofanimalsrequiredrequiredRefinementofexperimentalmethodstoofexperimentalmethodstoreducepainandstressoftheanimalreducepainandstressoftheanimalAvailabilitySecuringanimalsofaparticularspecies,strain,type,orageisveryimportantforaspecificproject.Indevelopedcountries（Indevelopedcountries（UnitedStates,Canada,UnitedStates,Canada,Japan,someEuropeancountriesJapan,someEuropeancountries）,itisnotaproblem.）,itisnotaproblem.IndevelopingcountriesitcanbeamajorIndevelopingcountriesitcanbeamajorproblem,especiallyforhealthyresearchproblem,especiallyforhealthyresearchanimals.animals.HousingRequirementsYourfacilitymaynothavethecapacityforcertainYourfacilitymaynothavethecapacityforcertainanimals（suchasprimatesorgoats）.Itisbettertoanimals（suchasprimatesorgoats）.Itisbettertochooseanotherspeciesatyourinstitutionifthechooseanotherspeciesatyourinstitutioniftheprojectallowsbecause:

@#@projectallowsbecause:

@#@itavoidstravelingtoanotherplace;@#@itavoidstravelingtoanotherplace;@#@youcanhavecloseandeasyobservation,youcanhavecloseandeasyobservation,animaltreatment,anddatamanagement;@#@andanimaltreatment,anddatamanagement;@#@anditisgenerallycheaper.itisgenerallycheaper.EaseofhandlingAllbasedontheweight:

@#@Allbasedontheweight:

@#@MiceandRats（20-500g）MiceandRats（20-500g）Rabbits（2-5kg）Rabbits（2-5kg）Dogs（20-30kg）Dogs（20-30kg）Pigs（20-50kg）Pigs（20-50kg）Goatsorsheep（50-80kg）Goatsorsheep（50-80kg）CostsThecostsforanimalpurchasing,Thecostsforanimalpurchasing,transportation,housing,unexpectedloss,transportation,housing,unexpectedloss,surgicalsupplyandservices,radiography,surgicalsupplyandservices,radiography,mechanicaltesting,andhistologyarebasic.mechanicaltesting,andhistologyarebasic.Financiallimitations:

@#@thesizeofthegrant,theFinanciallimitations:

@#@thesizeofthegrant,themoneyavailable.moneyavailable.UseacheaperanimalspeciesifitisvalidforUseacheaperanimalspeciesifitisvalidforverifyingthehypothesisverifyingthehypothesisCostsarenotdecisivefactorsforanimalselection.Costsarenotdecisivefactorsforanimalselection.DiseasesSpontaneousdiseasesinanimalscanSpontaneousdiseasesinanimalscanseriouslycompromisetheexperimentalseriouslycompromisetheexperimentalplan,confuseresearchdata,andraisetheplan,confuseresearchdata,andraisethecost.Researchersshouldavoidusingspeciesknowncost.Researchersshouldavoidusingspeciesknowntohavehighincidenceofparticulardiseases.tohavehighincidenceofparticulardiseases.Examples:

@#@Examples:

@#@ConventlConventl.r