NEB内切酶产品具体Buffer活性表.docx
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NEB内切酶产品具体Buffer活性表
NEBufferActivityChartforRestrictionEnzymes
NewEnglandBiolabsprovidesacolor-coded10XNEBufferwitheveryrestrictionendonucleasetoensure100%activity.Tohelpselectthebestconditionsfordoubledigests,thischartshowstheoptimal（supplied）NEBufferandapproximateactivityinthefourstandardNEBuffersforeachenzyme.IfBSAissuppliedwithanenzyme,itisincludedinallNEBufferactivityreactions.Inaddition,thetablealsoshowsrecommendedreactiontemperature,heatinactivationtemperatureandrecommendeddiluentbuffer.
Pleaseclickhereforadditionalinformationabout:
UniqueNEBufferActivity |DoubleDigestion|HeatInactivation|Activityat37°C|DiluentBuffers|Time-SaverEnzymes|HighFidelity（HF）RestrictionEnzymes
Thischarthasbeenupdatedtoreflectthechangeof54restrictionenzymestoNEBuffer4onNov.1,2008.
ClickfortheChartLegend,IconDescription
A|B|C|D|E|F|H|I|K|M|N|P|R|S|T|X|Z|
Enzyme
SuppliedNEBuffer
%ActivityinNEBuffers
Heat
Inactiva-
tion°C
Incuba.
Temp.
°C
Diluent
1
2
3
4
AatII
NEBuffer4
0
50
50
100
65
37
A
Acc65I
NEBuffer3+BSA
10
75
100
25
65
37
A
AccI
NEBuffer4
50
50
10
100
80
37
A
AciI
NEBuffer3
25
50
100
50
65
37
A
AclI
NEBuffer4+BSA
10
10
0
100
No
37
B
AcuI
NEBuffer4+SAM
50
100
50
100
65
37
B
AfeI
NEBuffer4
25
50
25
100
65
37
A
AflII
NEBuffer4+BSA
50
100
25
100
65
37
A
AflIII
NEBuffer3+BSA
25
75
100
50
80
37
B
AgeI
NEBuffer1
100
50
10
75
65
37
C
AhdI
NEBuffer4+BSA
25
75
0
100
65
37
A
AleI
NEBuffer4
10
25
10
100
65
37
A
AluI
NEBuffer4
100
100
75
100
65
37
B
AlwI
NEBuffer4
50
100
10
100
65
37
A
AlwNI
NEBuffer4
50
100
50
100
65
37
A
ApaI
NEBuffer4+BSA
25
50
0
100
65
25
A
ApaLI
NEBuffer4+BSA
100
100
10
100
No
37
A
ApeKI
NEBuffer3
25
75
100
50
No
75
B
ApoI
NEBuffer3+BSA
10
75
100
75
80
50
A
AscI
NEBuffer4
0
10
10
100
65
37
A
AseI
NEBuffer3
NR
75
100dd
NR
65
37
B
AsiSI
NEBuffer4+BSA
50
100
100
100
80
37
B
AvaI
NEBuffer4
10
75
10
100
80
37
A
AvaII
NEBuffer4
50
75
10
100
65
37
A
AvrII
NEBuffer4
100
100
50
100
80
37
B
Enzyme top
SuppliedNEBuffer
%inB1
%inB2
%inB3
%inB4
Heat
Inactiva.
Incuba.
Temp.
Diluent
BaeGI
NEBuffer1
100
75
10
50
65
37
A
BaeI
NEBuffer4+BSA+SAM
50
100
50
100
65
25
A
BamHI
NEBuffer3+BSA
75
100
100dd
100
No
37
A
BamHI-HF™
NEBuffer4
100
50
10
100
No
37
A
BanI
NEBuffer4+BSA
50
100
50
100
65
37
A
BanII
NEBuffer4
100
10
50
100
80
37
A
BbsI
NEBuffer2
100
100
25
75
65
37
B
BbvCI
NEBuffer4
50
100
10
100
80
37
A
BbvI
NEBuffer2
100
100
25
75
65
37
B
BccI
NEBuffer1+BSA
100
50
10
50
65
37
A
BceAI
NEBuffer3+BSA
100
100
100
100
65
37
A
BcgI
NEBuffer3+SAM
NR
NR
100dd
NR
65
37
A
BciVI
NEBuffer4
100
50
0
100
65
37
C
BclI
NEBuffer3
50
100
100dd
75
No
50
A
BfaI
NEBuffer4
75
50
10
100
80
37
B
BfuAI
NEBuffer3
0
75
100
10
65
50
B
BfuCI
NEBuffer4+BSA
100
50
25
100
80
37
B
BglI
NEBuffer3
50
75
100
50
65
37
B
BglII
NEBuffer3
10
75
100
10
No
37
A
BlpI
NEBuffer4
50
100
10
100
No
37
A
BmgBI
NEBuffer3+BSA
0
25
100
10
65
37
C
BmrI
NEBuffer2
75
100
75
100
65
37
B
BmtI
NEBuffer2
25
100
25
50
65
37
B
BpmI
NEBuffer3+BSA
75
100
100
100
65
37
B
Bpu10I
NEBuffer3
10
25
100
25
80
37
B
BpuEI
NEBuffer4+SAM
50
100
10
100
65
37
B
BsaAI
NEBuffer4
100
100
100
100
No
37
C
BsaBI
NEBuffer4
50
100
75
100
80
60
B
BsaHI
NEBuffer4+BSA
50
100
100
100
80
37
A
BsaI
NEBuffer4
75
75
100
100
65
50
B
BsaJI
NEBuffer4
100
100
100
100
80
60
A
BsaWI
NEBuffer4+BSA
50
100
50
100
80
60
A
BsaXI
NEBuffer4
75
100
10
100
No
37
B
BseRI
NEBuffer4
100
100
75
100
65
37
A
BseYI
NEBuffer3
10
50
100
50
65
37
B
BsgI
NEBuffer4+SAM
50
75
50
100
65
37
B
BsiEI
NEBuffer4+BSA
50
100
10
100
80
60
A
BsiHKAI
NEBuffer4+BSA
50
100
100
100
No
65
B
BsiWI
NEBuffer3
100
100
100
25
80
55
A
BslI
NEBuffer3
10
50
100
75
80
55
A
BsmAI
NEBuffer4
50
100
100
100
80
55
B
BsmBI
NEBuffer3
75
100
100
100
80
55
B
BsmFI
NEBuffer4+BSA
25
50
50
100
80
65
A
BsmI
NEBuffer4
75
100
75
100
80
65
A
BsoBI
NEBuffer2
10
100
100
50
No
37
A
Bsp1286I
NEBuffer4+BSA
25
25
25
100
65
37
A
BspCNI
NEBuffer4+BSA+SAM
100
75
10
100
80
25
A
BspDI
NEBuffer4
25
75
50
100
65
37
A
BspEI
NEBuffer3
0
10
100
0
80
37
B
BspHI
NEBuffer4
10
100
50
100
65
37
A
BspMI
NEBuffer3
NR
NR
100
NR
65
37
B
BspQI
NEBuffer4
10
50
100
100
80
50
B
BsrBI
NEBuffer4
50
100
100
100
80
37
A
BsrDI
NEBuffer2+BSA
50
100
50
75
80
65
A
BsrFI
NEBuffer4
10
100
100
100
No
37
C
BsrGI
NEBuffer2+BSA
25
100
10
100
80
37
A
BsrI
NEBuffer3
0
50
100
10
80
65
B
BssHII
NEBuffer3
100
100
100dd
100
80
50
A
BssKI
NEBuffer3+BSA
0
50
100
50
80
60
A
BssSI
NEBuffer3+BSA
0
50
100dd
10
80
37
B
BstAPI
NEBuffer4+BSA
25
100
100
100
80
60
A
BstBI
NEBuffer4
75
50
25
100
No
65
A
BstEII
NEBuffer3+BSA
50
75
100
75
No
60
A
BstNI
NEBuffer2+BSA
10
100
100
75
No
60
A
BstUI
NEBuffer4
100
100
50
100
No
60
A
BstXI
NEBuffer3
0
50
100
25
65
37
B
BstYI
NEBuffer2
50
100
75
100
80
60
A
BstZ17I
NEBuffer4
NR
NR
100
100
No
37
B
Bsu36I
NEBuffer3+BSA
0
25
100
0
80
37
A
BtgI
NEBuffer3+BSA
25
50
100
100
80
37
A
BtgZI
NEBuffer4+BSA
10
25
0
100
80
60
A
BtsCI
NEBuffer4
50
100
50
100
No
50
B
BtsI
NEBuffer4+BSA
100
50
50
100
80
55
A
Enzyme top
SuppliedNEBuffer
%inB1
%inB2
%inB3
%inB4
Heat
Inactiva.
Incuba.
Temp.
Diluent
Cac8I
NEBuffer4
50
75
100
100
65
37
B
ClaI
NEBuffer4+BSA
10
50
50
100
65
37
A
CspCI
NEBuffer4+SAM
10
100
10
100
65
37
A
CviAII
NEBuffer4
75
25
10
100
65
25
B
CviKI-1
NEBuffer4
10
50
25
100
80
37
A
CviQI
NEBuffer3+BSA
75
100
100
75
No
25
B
Enzyme top
SuppliedNEBuffer
%inB1
%inB2
%inB3
%inB4
Heat
Inactiva.
Incuba.
Temp.
Diluent
DdeI
NEBuffer3
75
100
100
75
65
37
A
DpnI
NEBuffer4
100
100
75
100
80
37
B
DpnII
NEBufferDpnII
NR
NR
100dd
NR
65
37
B
DraI
NEBuffer4
75
75
50
100
65
37
A
DraIII
NEBuffer3+BSA
100
75
100
25
65
37
B
DrdI
NEBuffer4+BSA
25
50
10
100
65
37
A
Enzyme top
SuppliedNEBuffer
%inB1
%inB2
%inB3
%inB4
Heat
Inactiva.
Incuba.
Temp.
Diluent
EaeI
NEBuffer4
100
100
50
100
80
37
A
EagI
NEBuffer3
10
25
100
10
65
37
C
EagI-HF™
NEBuffer4
25
50
10
100
65
37
C
EarI
NEBuffer4
100
100
50
100
65
37
A
EciI
NEBuffer2+BSA
50
100
100
50
65
37
A
EcoNI
NEBuffer4
100
100
75
100
65
37
A
EcoO109I
NEBuffer4+BSA
100
100
75
100
65
37
A
EcoP15I
NEBuffer3+BSA+ATP
75
100
100
100
65
37
A
EcoRI
NEBufferEcoRIdd
100
100
100
100
65
37
C
EcoRI-HF™
NEBuffer4
10
100
0
100
65
37
C
EcoRV
NEBuffer3+BSA
50
75
100
50
80
37
A
EcoRV-HF™
NEBuffer4
25
100
100
100
65
37
B
Enzyme top
SuppliedNEBuffer
%inB1
%inB2
%inB3
%inB4
Heat
Inactiva.
Incuba.
Temp.
Diluent
FatI
NEBuffer2
10
100
50
50
65
55
A
FauI
NEBuffer4
100
50
10
100
65
55
A
Fnu4HI
NEBuffer4
10
25
25
100
65
37
A
FokI
NEBuffer4
100
100
75
100
65
37
A
FseI
NEBuffer4+BSA
100
75
0
100
65
37
B
FspI
NEBuffer4
10
75
10
100dd
65
37
C
Enzyme top
SuppliedNEBuffer
%inB1
%inB2
%inB3
%inB4
Heat
Inactiva.
Incuba.
Temp.
Diluent
HaeII
NEBuffer4+BSA
75
100
50
100
80
37
A
HaeIII
NEBuffer4
50
100
25
100
80
37
A
HgaI
NEBuffer1
100
75
50
100
65
37
A
HhaI
NEBuffer4+BSA
75
100
100
100
65
37
A
HincII
NEBuffer3+BSA
75
100
100
100
65
37
B
HindIII
NEBuffer2
50
100
10
50
65
37
B
HinfI
NEBuffer4
75
100
75
100
80
37
A
HinP1I
NEBuffer4
100
100
100
100
65
37
A
HpaI
NEBuffer4
25
50
10
100
No
37
A
HpaII
NEBuffer1
100
50
10
50
65
37
A
HphI
NEBuffer4
NR
75
0
100
65
37
B
Hpy166II
NEBuffer4
100
100
50
100
65
37
C
Hpy188I
NEBuffer4
50
25
10
100
65
37
A
Hpy188III
NEBuffer4+BSA
100
100
10
100
65
37
A
Hpy99I
NEBuffer4+BSA
10
10
0
100
65
37
A
HpyAV
NEBuffer4+BSA
100
100
25
100
65
37
B
HpyCH4III
NEBuffer4
100
50
25
100
80
37
A
HpyCH4IV
NEBuffer1
100
25
10
25
65
37
A
HpyCH4V
NEBuffer4
50
50
25
100
65
37
B
Enzyme top
SuppliedNEBuffer
%inB1
%inB2
%inB3
%inB4
Heat
Inactiva.
Incuba.
Temp.
Diluent
I-CeuI
NEBuffer4+BSA
10
10
0
100
65
37
B
I-SceI
NEBufferI-SceI+BSA
10
50
50
50
65
37
B
Enzyme top
SuppliedNEBuffer
%inB1
%inB2
%inB3
%inB4
Heat
Inactiva.
Incuba.
Temp.
Diluent
KasI
NEBuffer4+BSA
25
100
0
100
65
37
B
KpnI
NEBuffer1+BSA
100
75
0
50
No
37
A
Enzyme top
SuppliedNEBuf
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