常用荧光染料探针列表.docx

1、常用荧光染料探针列表这是来自于Salk的一个比较全的荧光染料列表，这些荧光染料可广泛用于流式细胞术以及荧光显微镜技术，汇集了各种荧光染料的特性，方便大家查找。可根据实际所用的检测平台、染料的最大激发光波长和最大发射光波长来选择合适的荧光染料用于实验。请注意这上面所显示的颜色可能会由于所用浏览器不同而有所不同，他们只是一个与实际颜色的近似值。染料名称Excitation(nm)Emission(nm)分子量备注信息Reactive and conjugated probesHydroxycoumarin325386331Succinimidyl esterAminocoumarin3504453

2、30Succinimidyl esterMethoxycoumarin360410317Succinimidyl esterCascade Blue(375);401423596HydrazidePacific Blue403455406MaleimidePacific Orange403551Lucifer yellow425528NBD466539294NBD-XR-Phycoerythrin (PE)480;565578240 kPE-Cy5 conjugates480;565;650670aka Cychrome, R670, Tri-Color, Quantum RedPE-Cy7

3、conjugates480;565;743767Red 613480;565613PE-Texas RedPerCP490675Peridinin chlorphyll proteinTruRed490,675695 conjugateFluorX494520587GE HealthcareFluorescein495519389FITC; pH sensitiveBODIPY-FL503512TRITC547572444TRITCX-Rhodamine570576548XRITCLissamine Rhodamine B570590Texas Red589615625Sulfonyl chl

4、orideAllophycocyanin (APC)650660104 kAPC-Cy7 conjugates650;755767PharRedAlexa Fluor系列荧光染料Alexa Fluor 350343442410Alexa Fluor 4054014211028Alexa Fluor 430434540702Alexa Fluor 488499519643QY Alexa Fluor 500503525700Alexa Fluor 514517542714Alexa Fluor 532530555724QY Alexa Fluor 5465615721079QY Alexa Fl

5、uor 5555535681250QY Alexa Fluor 568579603792QY Alexa Fluor 594591618820QY Alexa Fluor 6106106291285Alexa Fluor 6336326481200Alexa Fluor 6476526681300QY Alexa Fluor 6606636911100Alexa Fluor 6806807021150Alexa Fluor 7006967191400Alexa Fluor 7507527761300Alexa Fluor 7907828041750Cy系列荧光染料Cy2489506714QY

6、Cy3(512);550570;(615)767QY Cy3B558572;(620)658QY 581594;(640)1102QY Cy5(625);650670792QY 6756941128QY Cy7743767818QY 核酸相关探针染料Hoechst 33342343483616 AT-selective DAPI345455AT-selective Hoechst 33258345478624AT-selective SYTOX Blue431480400DNA Chromomycin A3445575CG-selective Mithramycin445575YOYO-149

7、15091271Ethidium Bromide493620394 Acridine Orange503530/640DNA/RNASYTOX Green504523600 DNA TOTO-1, TO-PRO-1509533Vital stain, TOTO: Cyanine DimerTO-PRO: Cyanine MonomerThiazole Orange510530Propidium Iodide (PI)536617LDS 751543;590712;607472 DNA (543ex/712em), RNA (590ex/607em) 7-AAD5466477-aminoacti

8、nomycin D, CG-selectiveSYTOX Orange547570500 DNA TOTO-3, TO-PRO-3642661DRAQ5647681,697413usable excitation down to 488细胞功能探针Indo-1361/330490/4051010AM ester. Low/High Ca+,Fluo-3506526855AM ester. pH 6DCFH50553552927Dichorodihydrofluorescein, oxidized formDHR505534346Dihydrorhodamine 123, oxidized fo

9、rm,light catalyzes oxidationSNARF548/579587/635pH 6/9荧光蛋白QYBRPSY66H360442Y66F360508EBFP380440monomerEBFP238344820monomerAzurite38344715monomerGFPuv385508T-Sapphire3995112625weak dimerCerulean4334752736weak dimermCFP4334751364monomerECFP4344773CyPet4354771859weak dimerY66W436485mKeima-Red4406203monom

10、erTagCFP45848029dimerAmCyan145848929tetramermTFP146249254dimerS65A471504Midoriishi Cyan47249525dimerWild Type GFP396,47550826kS65C479507TurboGFP48250226 k37dimerTagGFP48250534monomerS65L484510Emerald48750939weak dimer; (Invitrogen)S65T488511EGFP48850726k 34174weak dimerAzami Green49250541monomerZsGr

11、een1493505105k40tetramerTagYFP50852447monomerEYFP51452726k5160weak dimerTopaz51452757monomerVenus5155285315weak dimermCitrine5165295949monomerYPet5175308049weak dimerTurboYFP52553826 kdimerZsYellow152953913tetramerKusabira Orange54855931monomermOrange548562499monomermKO54855931122monomerTurboRFP5535

12、7426 k62dimertdTomato5545819598tandem dimerTagRFP55558450monomerDsRed monomer55658628k16monomerDsRed2 (RFP)563582110k24mStrawberry5745962615monomerTurboFP60257460226 k26dimerAsRed2576592110k13tetramermRFP158460730kmonomerJ-Red58461013dimermCherry5876101696monomerHcRed158861852kdimerKatusha58863523di

13、mermKate (TagFP635)58863515monomerTurboFP63558863526 k22dimermPlum59064953mRaspberry59862513monomer; faster photobleach than mPlum其他探针Monochlorobimane380461226Glutathione probeCalcein496517623pH 5Ex: Peak excitation wavelength (nm) Em: Peak emission wavelength (nm) QY: Quantum yield BR: Brightness; Extinction coefficient * Quantum yield / 1000 PS: Photostability; time to 50% brightness (sec) 光色波长(nm)代表波长 红（Red）780630700 橙（Orange）630600620 黄（Yellow）600570580 绿（Green）570500550 青（Cyan）500470500 蓝（Blue）470420470 紫（Violet）420380420