常用荧光染料探针列表.docx
《常用荧光染料探针列表.docx》由会员分享,可在线阅读,更多相关《常用荧光染料探针列表.docx(13页珍藏版)》请在冰豆网上搜索。
常用荧光染料探针列表
这是来自于Salk的一个比较全的荧光染料列表,这些荧光染料可广泛用于流式细胞术以及荧光显微镜技术,汇集了各种荧光染料的特性,方便大家查找。
可根据实际所用的检测平台、染料的最大激发光波长和最大发射光波长来选择合适的荧光染料用于实验。
请注意这上面所显示的颜色可能会由于所用浏览器不同而有所不同,他们只是一个与实际颜色的近似值。
染料名称
Excitation(nm)
Emission(nm)
分子量
备注信息
Reactiveandconjugatedprobes
Hydroxycoumarin
325
386
331
Succinimidylester
Aminocoumarin
350
445
330
Succinimidylester
Methoxycoumarin
360
410
317
Succinimidylester
CascadeBlue
(375);401
423
596
Hydrazide
PacificBlue
403
455
406
Maleimide
PacificOrange
403
551
Luciferyellow
425
528
NBD
466
539
294
NBD-X
R-Phycoerythrin(PE)
480;565
578
240k
PE-Cy5conjugates
480;565;650
670
akaCychrome,R670,Tri-Color,QuantumRed
PE-Cy7conjugates
480;565;743
767
Red613
480;565
613
PE-TexasRed
PerCP
490
675
Peridininchlorphyllprotein
TruRed
490,675
695
conjugate
FluorX
494
520
587
GEHealthcare
Fluorescein
495
519
389
FITC;pHsensitive
BODIPY-FL
503
512
TRITC
547
572
444
TRITC
X-Rhodamine
570
576
548
XRITC
LissamineRhodamineB
570
590
TexasRed
589
615
625
Sulfonylchloride
Allophycocyanin(APC)
650
660
104k
APC-Cy7conjugates
650;755
767
PharRed
AlexaFluor系列荧光染料
AlexaFluor350
343
442
410
AlexaFluor405
401
421
1028
AlexaFluor430
434
540
702
AlexaFluor488
499
519
643
QY
AlexaFluor500
503
525
700
AlexaFluor514
517
542
714
AlexaFluor532
530
555
724
QY
AlexaFluor546
561
572
1079
QY
AlexaFluor555
553
568
1250
QY
AlexaFluor568
579
603
792
QY
AlexaFluor594
591
618
820
QY
AlexaFluor610
610
629
1285
AlexaFluor633
632
648
1200
AlexaFluor647
652
668
1300
QY
AlexaFluor660
663
691
1100
AlexaFluor680
680
702
1150
AlexaFluor700
696
719
1400
AlexaFluor750
752
776
1300
AlexaFluor790
782
804
1750
Cy系列荧光染料
Cy2
489
506
714
QY
Cy3
(512);550
570;(615)
767
QY
Cy3B
558
572;(620)
658
QY
581
594;(640)
1102
QY
Cy5
(625);650
670
792
QY
675
694
1128
QY
Cy7
743
767
818
QY
核酸相关探针染料
Hoechst33342
343
483
616
AT-selective
DAPI
345
455
AT-selective
Hoechst33258
345
478
624
AT-selective
SYTOXBlue
431
480
~400
DNA
ChromomycinA3
445
575
CG-selective
Mithramycin
445
575
YOYO-1
491
509
1271
EthidiumBromide
493
620
394
AcridineOrange
503
530/640
DNA/RNA
SYTOXGreen
504
523
~600
DNA
TOTO-1,TO-PRO-1
509
533
Vitalstain,TOTO:
CyanineDimer
TO-PRO:
CyanineMonomer
ThiazoleOrange
510
530
PropidiumIodide(PI)
536
617
LDS751
543;590
712;607
472
DNA(543ex/712em),RNA(590ex/607em)
7-AAD
546
647
7-aminoactinomycinD,CG-selective
SYTOXOrange
547
570
~500
DNA
TOTO-3,TO-PRO-3
642
661
DRAQ5
647
681,697
413
usableexcitationdownto488
细胞功能探针
Indo-1
361/330
490/405
1010
AMester.Low/HighCa++,
Fluo-3
506
526
855
AMester.pH>6
DCFH
505
535
529
2'7'Dichorodihydrofluorescein,oxidizedform
DHR
505
534
346
Dihydrorhodamine123,oxidizedform,
lightcatalyzesoxidation
SNARF
548/579
587/635
pH6/9
荧光蛋白
QY
BR
PS
Y66H
360
442
Y66F
360
508
EBFP
380
440
monomer
EBFP2
383
448
20
monomer
Azurite
383
447
15
monomer
GFPuv
385
508
T-Sapphire
399
511
26
25
weakdimer
Cerulean
433
475
27
36
weakdimer
mCFP
433
475
13
64
monomer
ECFP
434
477
3
CyPet
435
477
18
59
weakdimer
Y66W
436
485
mKeima-Red
440
620
3
monomer
TagCFP
458
480
29
dimer
AmCyan1
458
489
29
tetramer
mTFP1
462
492
54
dimer
S65A
471
504
MidoriishiCyan
472
495
25
dimer
WildTypeGFP
396,475
508
26k
S65C
479
507
TurboGFP
482
502
26k
37
dimer
TagGFP
482
505
34
monomer
S65L
484
510
Emerald
487
509
39
weakdimer;(Invitrogen)
S65T
488
511
EGFP
488
507
26k
34
174
weakdimer
AzamiGreen
492
505
41
monomer
ZsGreen1
493
505
105k
40
tetramer
TagYFP
508
524
47
monomer
EYFP
514
527
26k
51
60
weakdimer
Topaz
514
527
57
monomer
Venus
515
528
53
15
weakdimer
mCitrine
516
529
59
49
monomer
YPet
517
530
80
49
weakdimer
TurboYFP
525
538
26k
dimer
ZsYellow1
529
539
13
tetramer
KusabiraOrange
548
559
31
monomer
mOrange
548
562
49
9
monomer
mKO
548
559
31
122
monomer
TurboRFP
553
574
26k
62
dimer
tdTomato
554
581
95
98
tandemdimer
TagRFP
555
584
50
monomer
DsRedmonomer
556
586
~28k
16
monomer
DsRed2("RFP")
563
582
~110k
24
mStrawberry
574
596
26
15
monomer
TurboFP602
574
602
26k
26
dimer
AsRed2
576
592
~110k
13
tetramer
mRFP1
584
607
~30k
monomer
J-Red
584
610
13
dimer
mCherry
587
610
16
96
monomer
HcRed1
588
618
~52k
dimer
Katusha
588
635
23
dimer
mKate(TagFP635)
588
635
15
monomer
TurboFP635
588
635
26k
22
dimer
mPlum
590
649
53
mRaspberry
598
625
13
monomer;fasterphotobleachthanmPlum
其他探针
Monochlorobimane
380
461
226
Glutathioneprobe
Calcein
496
517
623
pH>5
Ex:
Peakexcitationwavelength(nm)
Em:
Peakemissionwavelength(nm)
QY:
Quantumyield
BR:
Brightness;Extinctioncoefficient*Quantumyield/1000
PS:
Photostability;timeto50%brightness(sec)
光色波长λ(nm)代表波长
红(Red)780~630700
橙(Orange)630~600620
黄(Yellow)600~570580
绿(Green)570~500550
青(Cyan)500~470500
蓝(Blue)470~420470
紫(Violet)420~380420