常用缓冲溶液的配制.docx

常用缓冲溶液的配制.docx

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常用缓冲溶液的配制

1.甘氨酸-盐酸缓冲液（0.05M）Xml0.2M甘氨酸+Yml0.2M盐酸再加水稀释至200mlpHX/mlY/mlpHX/mlY/ml

2.25044.0

3.05011.4

2.45032.4

3.2508.2

2.65024.2

3.4506.4

2.85016.8

3.6505.0

甘氨酸分子量=75.070.2M甘氨酸溶液含15.01g/L

2.邻苯二甲酸-盐酸缓冲液（0.05M）

Xml0.2M邻苯二甲酸氢钾+Yml0.2M盐酸再加水稀释至200ml

pHXYpHXY

2.254.670

3.251.470

2.45

3.9603.450.990

2.65

3.2953.650.597

2.852.642

3.850.263

3.052.032

邻苯二甲酸氢钾分子量=2.4.230.2M邻苯二甲酸氢钾溶液含40.85g/L

3.磷酸氢二钠-柠檬酸缓冲液

pH0.2MNa2HPO4/ml0.1M柠檬酸/mlpH0.2MNa2HPO4/ml0.1M柠檬酸/ml

2.20.4019.65.210.729.28

2.41.2418.765.411.158.85

2.62.1817.825.611.608.40

2.8

3.1716.835.812.097.91

3.0

4.111

5.89

6.012.63

7.37

3.2

4.941

5.06

6.213.226.78

3.45.701

4.306.413.856.15

3.66.4413.566.61

4.55

5.45

3.87.1012.906.815.45

4.55

4.07.7112.297.016.473.53

4.28.2811.727.217.392.61

4.48.8211.187.418.171.83

4.69.3510.657.618.731.27

4.89.8610.147.819.150.85

5.010.309.708.019.450.55

Na2HPO4分子量=141.980.2M溶液含28.40g/L

Na2HPO4·2H2O分子量=178.050.2M溶液含35.61g/L

C6H8O7·H2O分子量=210.140.1M溶液含21.01g/L

4.柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度/M柠檬酸C6H8O7·H2O/g氢氧化钠NaOH/g浓盐酸HCl/ml终体积/L

2.20.202108416010

3.10.202108311610

3.30.202108310610

4.30.20210834510

5.30.352451446810

5.80.4528518610510

6.50.3826615612610

使用时可以每升中加入1g酚,若最后pH有变化,再用少量50%氢氧化钠溶液或浓盐酸调节,冰箱保存。

5.柠檬酸-柠檬酸钠缓冲液（0.1M）

pH0.1M柠檬酸/ml0.1M柠檬酸钠/mlpH0.1M柠檬酸/ml0.1M柠檬酸钠/ml

3.018.61.45.08.211.8

3.217.22.85.27.312.7

3.416.0

4.0

5.4

6.413.6

3.61

4.9

5.15.65.514.5

3.81

4.06.0

5.84.715.3

4.013.16.96.03.816.2

4.212.37.76.22.817.2

4.411.48.66.42.018.0

4.610.39.76.610418.6

4.89.210.8

C6H8O7·H2O分子量=210.140.1M溶液含21.01g/L

Na3C6H5O7·2H2O分子量=294.120.1M溶液含29.41g/L

6.乙酸-乙酸钠缓冲液（0.2M）pH0.2MNaAc/ml0.2MHAC/mlpH0.2MNaAc/ml0.2MHAC/ml

3.60.759.25

4.8

5.904.10

3.81.208.805.07.003.00

4.01.808.20

5.27.902.10

4.22.657.35

5.48.601.40

4.43.706.30

5.69.100.90

4.64.90

5.105.89.400.60

NaAc分子量=136.090.2M溶液为27.22g/L

7.磷酸盐缓冲液

（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2M）

pH0.2MNa2HPO4/ml0.2MNaH2PO4/mlpH0.2MNa2HPO4/ml0.2MNaH2PO4/ml

5.88.092.07.061.039.0

5.910.090.07.167.033.0

6.012.38

7.77.272.02

8.0

6.115.085.0

7.377.023.0

6.218.581.5

7.481.019.0

6.322.57

7.57.584.016.0

6.426.573.5

7.687.013.0

6.531.568.5

7.789.510.5

6.63

7.562.57.891.5

8.5

6.743.556.5

7.993.07.0

6.849.551.08.094.75.3

6.955.045.0

Na2HPO4·2H2O分子量=178.050.2M溶液含35.61g/L

Na2HPO4·12H2O分子量=358.220.2M溶液含71.64g/L

NaH2PO4·H2O分子量=138.010.2M溶液含27.6g/L

NaH2PO4·2H2O分子量=156.030.2M溶液含31.21g/L

（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15M）

pH1/15MNa2HPO4/ml1/15MKH2PO4/mlpH1/15MNa2HPO4/ml1/15MKH2PO4/ml

4.920.109.907.177.003.00

5.290.509.507.388.002.00

5.911.009.007.739.001.00

6.242.008.008.049.500.50

6.473.00

7.00

8.34

9.750.25

6.644.006.008.679.900.10

6.815.005.009.1810.000

6.986.004.00

Na2HPO4·2H2O分子量=178.051/15M溶液含35.61g/L

KH2PO4分子量=136.091/15M溶液含9.078g/L

8.磷酸二氢钾-氢氧化钠缓冲液（0.05M）Xml0.2MKH2PO4+Yml0.2MNaOH再加水稀释至20ml

pH（20℃）X/mlY/mlpH（20℃）X/mlY/ml

5.850.3727.052.963

6.050.570

7.253.500

6.250.860

7.453.950

6.451.260

7.654.280

6.651.780

7.854.520

6.852.3658.054.680

9.巴比妥钠-盐酸缓冲液（18℃）pH0.04M巴比妥钠/ml0.2M盐酸/mlpH0.04M巴比妥钠/ml0.2M盐酸/ml

6.810018.48.41005.21

7.010017.88.61003.82

7.210016.78.81002.52

7.410015.39.01001.65

7.610013.49.21001.13

7.810011.479.41000.70

8.01009.399.61000.35

8.21007.21100

巴比妥钠分子量=206.180.04M溶液为8.25g/L

10.Tris-盐酸缓冲液50ml0.1M三羟甲基氨基甲烷（Tris）溶液于Xml0.1M盐酸混匀后,加水稀释至100ml

pHX/mlpHX/ml

7.145.78.126.2

7.244.78.222.9

7.343.48.319.9

7.442.08.417.2

7.540.38.514.7

7.638.58.612.4

7.736.68.710.3

7.834.58.88.5

7.932.08.97.0

8.029.2

三羟甲基氨基甲烷（Tris）分子量=121.140.1M溶液为12.114g/L

Tris溶液可以从空气中吸收二氧化碳,使用时注意将瓶盖严

11.硼酸-硼砂缓冲液（0.2M硼酸根）

pH0.05M硼砂/ml0.2M硼酸/mlpH0.05M硼砂/ml0.2M硼酸/ml

7.41.09.08.23.56.5

7.61.58.58.44.55.5

7.82.08.08.76.04.0

8.03.07.09.08.02.0

硼砂Na2B4O7·10H2O分子量=381.430.05M（=0.2M硼酸根）溶液为19.07g/L

硼酸H3BO3分子量=61.840.2M溶液为12.37g/L

硼砂易失去结晶水,必须在带塞的瓶中保存

12.甘氨酸-氢氧化钠缓冲液（0.05M）

Xml0.2M甘氨酸+Yml0.2M氢氧化钠再加水稀释至200ml

pHX/mlY/mlpHX/mlY/ml

8.6504.09.65022.4

8.8506.09.85027.2

9.0508.810.05032.0

9.25012.010.45038.6

9.45016.810.65045.5

甘氨酸分子量=75.070.2M甘氨酸溶液含15.01g/L

13.硼砂-氢氧化钠缓冲液（0.05M硼酸根）Xml0.05M硼砂+Yml0.2M氢氧化钠再加水稀释至200ml

pHX/mlY/mlpHX/mlY/ml

9.3506.09.85034.0

9.45011.010.05043.0

9.65023.010.15046.0

硼砂Na2B4O7·10H2O分子量=381.430.05M（=0.2M硼酸根）溶液为19.07g/L

14.碳酸钠-碳酸氢钠缓冲液（0.1M）

Ca2+,Mg2+存在时不得使用

pH0.1M碳酸钠/ml0.1M碳酸氢钠/ml

20℃37℃

9.168.7719

9.409.1228

9.519.4037

9.789.5046

9.909.7255

10.149.9064

10.2810.0873

10.53102882

10.83105791

Na2CO3·10H2O分子量=286.20.1M溶液为28.62g/L

NaHCO3分子量=84.00.1M溶液为8.40g/L