NEB 同尾酶表.docx
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NEB同尾酶表
连接互相匹配的粘性末端,产生新的酶切位点
通常连接互相匹配的粘性末端可以产生新的酶切位点。
以下组合是通过计算机程序计算出来的,尽管我们希望能尽量保证其准确性,但有些未经实验证实,因此不能保证100%的可行性。
如果有同裂酶存在,则只列出其中的一个酶。
本表中列出的切酶均为已商品化的切酶。
识别序列不够严谨的切酶(例如,识别多个序列的酶)只在括号标出相关的序列,但注意这些酶不只切割该序列。
"-"表示连接产物不能被再切割。
酶
连接到
可进行切割的酶
Acc65I
(G/GTACC)
BanI(G/GTACC)
Acc65I,BanI,KpnI,NlaIV,RsaI
BsiWI,BsrGI
RsaI
AccI
(GT/CGAC)
AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI
--
(GT/CGAC)
ClaI,BstBI,TaqI
TaqI
AciI
(C/CGC)
AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI
--
BsaHI(GR/CGCC),HinP1I,NarI
AciI
HpaII
HpaII
AclI
(AA/CGTT)
AccI(GT/CGAC),AciI,ClaI,BstBI,HinP1I,HpaII,NarI,TaqI
--
AflIII
(A/CGCGT)
AscI,BssHII
BstUI,HhaI
(A/CATGT)
BspHI,NcoI,PciI
NlaIII
(A/CGCGT)
MluI
AflIII,BstUI,MluI
AgeI
(A/CCGGT)
AvaI(C/CCGGG),XmaI
HpaII,NciI,ScrFI
BsaWI,BspEI
BsaWI,HpaII
BsrFI(A/CCGGT),SgrAI(CA/CCGGTG)
AgeI,BsaWI,BsrFI,HpaII
NgoMIV
BsrFI,HpaII
ApaI
(GGGCC/C)
BanII(GGGCC/C),Bsp1286I(GGGCC/C)
ApaI,BanII,Bsp120I,Bsp1286I,HaeIII,NlaIV,Sau96I
ApaLI
(G/TGCAC)
SfcI(C/TGCAG)
BsgI
ApoI
(A/AATTY)
EcoRI
ApoI,Tsp509I
(G/AATTY)
EcoRI
ApoI,EcoRI,Tsp509I
(R/AATTY)
MfeI,Tsp509I
Tsp509I
AscI
(GG/CGCGCC)
AflIII(A/CGCGT),MluI
BstUI,HhaI
BssHII
BssHII,BstUI,Cac8I,HhaI
AseI
(AT/TAAT)
BfaI,Csp6I,NdeI
--
MseI
MseI
AsiSI
(GCGAT/CGC)
BsiEI(CGAT/CG)
DpnII,PvuI
PacI
MseI
PvuI
DpnII,PvuI
AvaI
(C/CCGGG)
AgeI,BsaWI,BspEI,BsrFI(R/CCGGY),NgoMIV,SgrAI(CR/CCGGYG)
HpaII,NciI,ScrFI
(C/TCGAG)
XhoI
AvaI,TaqI,XhoI
(C/TCGAG)
SalI
TaqI
(C/CCGGG)
XmaI
AvaI,BsaJI,HpaII,NciI,ScrFI,SmaI
AvaII
(G/GWCC)
PpuMI(RG/GACCY)
AvaII,NlaIV,Sau96I
RsrII
AvaII,Sau96I
PpuMI(RG/GTCCY)
AvaII,BsmFI,NlaIV,Sau96I
AvrII
(C/CTAGG)
NheI,SpeI,XbaI
BfaI
StyI(C/CTAGG)
AvrII,BfaI,BsaJI,StyI
BamHI
(G/GATCC)
BclI,DpnII
AlwI,DpnII
BglII,BstYI(R/GATCY)
AlwI,BstYI,DpnII
BstYI(G/GATCC)
AlwI,BamHI,BstYI,DpnII,NlaIV
BanI
(G/GTACC)
Acc65I
Acc65I,BanI,KpnI,NlaIV,RsaI
(G/GCGCC)
KasI
BanI,BsaHI,HaeII,HhaI,KasI,NarI,NlaIV
(G/GTACC)
BsiWI,BsrGI
RsaI
BanII
(GGGCC/C)
ApaI,Bsp1286I(GGGCC/C)
ApaI,BanII,Bsp1286I,HaeIII,NlaIV,Sau96I
(GAGCT/C)
Bsp1286I(GAGCT/C),SacI
AluI,BanII,BsiHKAI,Bsp1286I,SacI
BclI
(T/GATCA)
BamHI,BstYI(R/GATCY)
AlwI,DpnII
BglII,MboII
DpnII
BfaI
(C/TAG)
AseI,Csp6I,MseI,NdeI
--
BglII
(A/GATCT)
BamHI,BstYI(R/GATCY)
AlwI,BstYI,DpnII
BclI,DpnII
DpnII
BsaHI
(GR/CGYC)
AccI(GT/CGAC),ClaI,BstBI,TaqI
--
(GA/CGYC)
AciI,HinP1I
HgaI
(GG/CGYC)
AciI,HinP1I
HhaI
(GG/CGYC)
HpaII
AciI
(GA/CGYC)
NarI
BsaHI,HgaI
(GG/CGYC)
NarI
BanI,BsaHI,HaeII,HhaI,NarI,NlaIV
BsaWI
(W/CCGGW)
AgeI,BsrFI(R/CCGGY),SgrAI(CR/CCGGYG)
AgeI,BsaWI,BsrFI,HpaII
AvaI(C/CCGGG),XmaI
HpaII,NciI,ScrFI
BspEI
BsaWI,BspEI,HpaII
BsrFI(R/CCGGY),NgoMIV
BsrFI,HpaII
NgoMIV
HpaII
BsiEI
(CGAT/CG)
PacI
MseI
(CGAT/CG)
PvuI
BsiEI,DpnII,PvuI
(CGGC/CG)
SacII
AciI
BsiHKAI
(GTGCA/C)
Bsp1286I(GTGCA/C)
BsiHKAI,Bsp1286I
Bsp1286I(GAGCA/C)
BsiHKAI,Bsp1286I
Bsp1286I(GAGCT/C),SacI
AluI,BanII,BsiHKAI,Bsp1286I,SacI
NsiI
--
PstI,SbfI
BsgI
BsiWI
(C/GTACG)
Acc65I,BanI(G/GTACC),BsrGI
RsaI
Bsp1286I
(GGGCC/C)
ApaI,BanII(GGGCC/C)
ApaI,BanII,Bsp1286I,HaeIII,NlaIV,Sau96I
(GTGCA/C)
BsiHKAI
ApaLI,BsiHKAI,Bsp1286I
(GGGCC/C)
BanII(GGGCC/C)
BanII,Bsp1286I
(GAGCT/C)
BanII(GAGCT/C),BsiHKAI,SacI
AluI,BanII,BsiHKAI,Bsp1286I,SacI
(GWGCW/C)
BsiHKAI
BsiHKAI,Bsp1286I
(GTGCA/C)
NsiI
--
(GTGCA/C)
PstI,SbfI
BsgI
BspEI
(T/CCGGA)
AgeI,BsaWI,BsrFI(R/CCGGY),SgrAI(CR/CCGGYG)
BsaWI,HpaII
AvaI(C/CCGGG),XmaI
HpaII,NciI,ScrFI
BsaWI
BsaWI,BspEI,HpaII
BsrFI(R/CCGGY),NgoMIV
HpaII
BspHI
(T/CATGA)
AflIII(A/CATGT),NcoI,StyI(C/CATGG)
NlaIII
BsrFI
(A/CCGGY)
AgeI,BsaWI
AgeI,BsaWI,BsrFI,HpaII
(G/CCGGY)
AgeI,BsaWI,NgoMIV
BsrFI,HpaII
(R/CCGGY)
AvaI(C/CCGGG),XmaI
HpaII,NciI,ScrFI
(A/CCGGY)
BsaWI,BspEI
BsaWI,HpaII
(R/CCGGY)
BsaWI,BspEI
HpaII
(G/CCGGY)
NgoMIV
BsrFI,Cac8I,HpaII,NaeI
(CR/CCGGYG)
SgrAI
BsrFI,HpaII
BsrGI
(T/GTACA)
Acc65I,BanI(G/GTACC),BsiWI
RsaI
BssHII
(G/CGCGC)
AflIII(A/CGCGT),MluI
BstUI,HhaI
AscI
BssHII,BstUI,Cac8I,HhaI
BstBI
(TT/CGAA)
AccI(GT/CGAC),ClaI,TaqI
TaqI
AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI
--
BstYI
(A/GATCY)
BamHI,BglII
AlwI,BstYI,DpnII
(G/GATCY)
BamHI
AlwI,BamHI,BstYI,DpnII,NlaIV
(R/GATCY)
BclI,DpnII
DpnII
(G/GATCY)
BclI,DpnII
AlwI,DpnII
(A/GATCY)
BglII
BglII,BstYI,DpnII
ClaI
(AT/CGAT)
AccI(GT/CGAC),BstBI,TaqI
TaqI
AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI
--
DpnII/MboI/
Sau3AI(/GATC)
BamHI,BstYI(R/GATCC)
AlwI,DpnII
BclI,BglII,BstYI(R/GATCY)
DpnII
EaeI
(Y/GGCCR)
PspOMI
HaeIII,Sau96I
(C/GGCCR)
EagI
BsiEI,EaeI,EagI,HaeIII
(T/GGCCR)
EagI
EaeI,HaeIII
(C/GGCCR)
NotI
AciI,BsiEI,EaeI,EagI,Fnu4HI,HaeIII
(T/GGCCR)
NotI
AciI,EaeI,Fnu4HI,HaeIII
EagI
(C/GGCCG)
PspOMI
HaeIII,Sau96I
EaeI(Y/GGCCR)
EaeI,HaeIII
EaeI(C/GGCCG)
BsiEI,EaeI,EagI,HaeIII
NotI
AciI,BsiEI,EaeI,EagI,Fnu4HI,HaeIII
EcoRI
(G/AATTC)
ApoI(G/AATTC)
ApoI,EcoRI,Tsp509I
ApoI(R/AATTY)
ApoI,Tsp509I
MfeI,Tsp509I
Tsp509I
HinP1I
(G/CGC)
AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI
--
AciI,BsaHI(GR/CGCC),NarI
HhaI
BsaHI(GR/CGTC)
HgaI
HpaII
AciI
HpaII/MspI
(C/CGG)
AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI
--
AciI,BsaHI(GR/CGCC),HinP1I,NarI
AciI
KasI
(G/GCGCC)
BanI(G/GCGCC)
BanI,BsaHI,HaeII,HhaI,KasI,NarI,NlaIV
MfeI
(C/AATTG)
ApoI(R/ATTTY),EcoRI,Tsp509I
Tsp509I
MluI
(A/CGCGT)
AflIII(A/CGCGT)
AflIII,BstUI,MluI
AscI,BssHII
BstUI,HhaI
MseI
(T/TAA)
AseI
MseI
BfaI,Csp6I,NdeI
--
NarI
(GG/CGCC)
AccI(GT/CGAC),AclI,ClaI,BstBI,TaqI
--
AciI,HinP1I
HhaI
BsaHI(GR/CGCC)
BanI,BsaHI,HaeII,HhaI,NarI,NlaIV
BsaHI(GR/CGTC)
BsaHI,HgaI
HpaII
AciI
NcoI
(C/CATGG)
AflIII(A/CATGT),BspHI
NlaIII
StyI(C/CATGG)
BsaJI,NcoI,NlaIII,StyI
PciI
NlaIII
NdeI
(CA/TATG)
AseI,BfaI,Csp6I,MseI
--
NgoMIV
(G/CCGGC)
AgeI,BsaWI,BsrFI(R/CCGGY),SgrAI
BsrFI,HpaII
AvaI(C/CCGGG),XmaI
HpaII,NciI,ScrFI
BsaWI,BspEI
HpaII
BsrFI(R/CCGGC),SgrAI
BsrFI,Cac8I,HpaII,NaeI
NheI
(G/CTAGC)
AvrII,SpeI,StyI(C/CTAGG),XbaI
BfaI
NlaIII
(CATG/)
SphI,NspI
NlaIII
NotI
(GC/GGCCGC)
PspOMI
AciI,EaeI,Fnu4HI,HaeIII
EagI
AciI,BsiEI,EaeI,EagI,Fnu4HI,HaeIII
EaeI(Y/GGCCR)
AciI,BsiEI,EaeI,Fnu4HI,HaeIII
NsiI
(ATGCA/T)
BsiHKAI(GTGCA/C),Bsp1286I(GTGCA/C),PstI,SbfI
--
NspI
(RCATG/Y)
NlaIII,SphI
NlaIII
PacI
(TTAAT/TAA)
BsiEI(CGAT/CG),PvuI
MseI
PciI
(A/CATGT)
AflII(A/CATGT),BspHI,NcoI,StyI(C/CTAGG)
NlaIII
PpuMI
(RG/GWCCY)
AvaII,RsrII
AvaII,Sau96I
(GG/GTCCY)
AvaII,RsrII
AvaII,BsmFI,NlaIV,Sau96I
(GG/GACCY)
AvaII,RsrII
AvaII,NlaIV,Sau96I
PspOMI
(G/GGCCC)
EaeI(Y/GGCCR),EagI
HaeIII,Sau96I
NotI
AciI,Fnu4HI,HaeIII,Sau96I
PstI
(CTGCA/G)
BsiHKAI,Bsp1286I(GTGCA/C)
BsgI
NsiI
--
SbfI
PstI
PvuI
(CGAT/CG)
AsiSI
DpnI,PvuI
PacI
MseI
BsiEI(CGAT/CG)
BsiEI,DpnII,PvuI
RsrII
(CG/GWCCG)
AvaII,PpuMI(RG/GACCY)
AvaII,Sau96I
PpuMI(RG/GACCY)
AvaII,NlaIV,Sau96I
PpuMI(RG/GTCCY)
AvaII,BsmFI,NlaIV,Sau96I
SacI
(GAGCT/C)
BanII(GAGCT/C),BsiHKAI,Bsp1286I(GAGCT/C)
AluI,BanII,BsiHKAI,Bsp1286I,SacI
SacII
(CCGC/GG)
BsiEI(CGGC/CG)
AciI
SalI
(G/TCGAC)
AvaI(C/TCGAG),XhoI
TaqI
SbfI
(CCTGCA/GG)
BsiHKAI,Bsp1286I(GTGCA/C)
BsgI
NsiI
--
PstI
PstI
SfcI
(C/TGCAG)
ApaLI
BsgI
SgrAI
(CR/CCGGYG)
SeeBsrFI
SpeI
(A/CTAGT)
AvrII,NheI,StyI(C/CTAGG),XbaI
BfaI
SphI
(GCATG/C)
NlaIII,NspI
NlaIII
StyI
AvrII
AvrII,BfaI,BsaJI,StyI
(C/CTAGG)
NheI,SpeI,XbaI
BfaI
(C/CATGG)
BspHI
NlaIII
NcoI
BsaJI,NcoI,NlaIII,StyI
TaqI
(T/CGA)
AccI(GT/CGAC),ClaI,BstBI
TaqI
AciI,AclI,BsaHI(GR/CGYC),HinP1I,HpaII,NarI
--
Tsp509I
(/AATT)
ApoI(R/AATTY),EcoRI,MfeI
Tsp509I
XbaI
(T/CTAGA)
AvrII,NheI,SpeI,StyI(C/CTAGG)
BfaI
XhoI/TliI
(C/TCGAG
AvaI(C/TCGAG)
AvaI,TaqI,XhoI
SalI
TaqI
XmaI
(C/CCGGG
AgeI,BsaWI,BspEI,BsrFI,NgoMIV,SgrAI
HpaII,NciI,ScrFI
AvaI(C/CCGGG)
AvaI,BsaJI,HpaII,NciI,ScrFI,SmaI,XmaI