pLVX-TetOne-Puro慢病毒载体使用说明_精品文档.pdf

pLVX-TetOne-Puro慢病毒载体使用说明_精品文档.pdf

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pLVX-TetOne-PuropLVX-TetOne-Puro载体基本信息载体基本信息：

载体名称载体名称:

pLVX-TetOne-Puro质粒类型质粒类型:

慢病毒载体；四环素调控载体慢病毒载体；四环素调控载体高拷贝高拷贝/低拷贝低拷贝:

高拷贝高拷贝克隆方法克隆方法:

限制性内切酶，多克隆位点限制性内切酶，多克隆位点启动子启动子:

TRE3GS载体大小载体大小:

9227bp5测序引物及序列测序引物及序列:

-3测序引物及序列测序引物及序列:

-载体标签载体标签:

无无载体抗性载体抗性:

氨苄青霉素氨苄青霉素筛选标记筛选标记:

嘌呤霉素（嘌呤霉素（Puromycin）克隆菌株克隆菌株:

Stbl3宿主细胞（系）宿主细胞（系）:

常规细胞系（常规细胞系（293、CV-1、CHO等）等）备注备注:

慢病毒载体慢病毒载体pLVX-TetOne-Puro是集调控与应答功能于一是集调控与应答功能于一体的四环素诱导载体。

体的四环素诱导载体。

稳定性稳定性:

稳表达稳表达组成型组成型/诱导型诱导型:

诱导型诱导型病毒病毒/非病毒非病毒:

慢病毒慢病毒pLVX-TetOne-Puro载体质粒图谱和多克隆位点信息载体质粒图谱和多克隆位点信息：

编号编号载体名称载体名称北京华越洋北京华越洋VECT231260pLVX-TetOne-PuropLVX-TetOne-Puro载体序列载体序列：

ORIGIN1TGGAAGGGCTAATTCACTCCCAAAGAAGACAAGATATCCTTGATCTGTGGATCTACCACA61CACAAGGCTACTTCCCTGATTAGCAGAACTACACACCAGGGCCAGGGGTCAGATATCCAC121TGACCTTTGGATGGTGCTACAAGCTAGTACCAGTTGAGCCAGATAAGGTAGAAGAGGCCA181ATAAAGGAGAGAACACCAGCTTGTTACACCCTGTGAGCCTGCATGGGATGGATGACCCGG241AGAGAGAAGTGTTAGAGTGGAGGTTTGACAGCCGCCTAGCATTTCATCACGTGGCCCGAG301AGCTGCATCCGGAGTACTTCAAGAACTGCTGATATCGAGCTTGCTACAAGGGACTTTCCG361CTGGGGACTTTCCAGGGAGGCGTGGCCTGGGCGGGACTGGGGAGTGGCGAGCCCTCAGAT421CCTGCATATAAGCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCTGA481GCCTGGGAGCTCTCTGGCTAACTAGGGAACCCACTGCTTAAGCCTCAATAAAGCTTGCCT541TGAGTGCTTCAAGTAGTGTGTGCCCGTCTGTTGTGTGACTCTGGTAACTAGAGATCCCTC601AGACCCTTTTAGTCAGTGTGGAAAATCTCTAGCAGTGGCGCCCGAACAGGGACTTGAAAG661CGAAAGGGAAACCAGAGGAGCTCTCTCGACGCAGGACTCGGCTTGCTGAAGCGCGCACGG721CAAGAGGCGAGGGGCGGCGACTGGTGAGTACGCCAAAAATTTTGACTAGCGGAGGCTAGA781AGGAGAGAGATGGGTGCGAGAGCGTCAGTATTAAGCGGGGGAGAATTAGATCGCGATGGG841AAAAAATTCGGTTAAGGCCAGGGGGAAAGAAAAAATATAAATTAAAACATATAGTATGGG901CAAGCAGGGAGCTAGAACGATTCGCAGTTAATCCTGGCCTGTTAGAAACATCAGAAGGCT961GTAGACAAATACTGGGACAGCTACAACCATCCCTTCAGACAGGATCAGAAGAACTTAGAT1021CATTATATAATACAGTAGCAACCCTCTATTGTGTGCATCAAAGGATAGAGATAAAAGACA1081CCAAGGAAGCTTTAGACAAGATAGAGGAAGAGCAAAACAAAAGTAAGACCACCGCACAGC1141AAGCGGCCGGCCGCTGATCTTCAGACCTGGAGGAGGAGATATGAGGGACAATTGGAGAAG1201TGAATTATATAAATATAAAGTAGTAAAAATTGAACCATTAGGAGTAGCACCCACCAAGGC1261AAAGAGAAGAGTGGTGCAGAGAGAAAAAAGAGCAGTGGGAATAGGAGCTTTGTTCCTTGG1321GTTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGC1381CAGACAATTATTGTCTGGTATAGTGCAGCAGCAGAACAATTTGCTGAGGGCTATTGAGGC1441GCAACAGCATCTGTTGCAACTCACAGTCTGGGGCATCAAGCAGCTCCAGGCAAGAATCCT1501GGCTGTGGAAAGATACCTAAAGGATCAACAGCTCCTGGGGATTTGGGGTTGCTCTGGAAA1561ACTCATTTGCACCACTGCTGTGCCTTGGAATGCTAGTTGGAGTAATAAATCTCTGGAACA1621GATTTGGAATCACACGACCTGGATGGAGTGGGACAGAGAAATTAACAATTACACAAGCTT1681AATACACTCCTTAATTGAAGAATCGCAAAACCAGCAAGAAAAGAATGAACAAGAATTATT1741GGAATTAGATAAATGGGCAAGTTTGTGGAATTGGTTTAACATAACAAATTGGCTGTGGTA1801TATAAAATTATTCATAATGATAGTAGGAGGCTTGGTAGGTTTAAGAATAGTTTTTGCTGT1861ACTTTCTATAGTGAATAGAGTTAGGCAGGGATATTCACCATTATCGTTTCAGACCCACCT1921CCCAACCCCGAGGGGACCCGACAGGCCCGAAGGAATAGAAGAAGAAGGTGGAGAGAGAGA1981CAGAGACAGATCCATTCGATTAGTGAACGGATCTCGACGGTATCGCCTTTAAAAGAAAAG2041GGGGGATTGGGGGGTACAGTGCAGGGGAAAGAATAGTAGACATAATAGCAACAGACATAC2101AAACTAAAGAACTACAAAAACAAATTACAAAAATTCAAAATTTTCGGGTTTATTACAGGG2161ACAGCAGAGATCCAGTTTATCGACTTAACTTGTTTATTGCAGCTTATAATGGTTACAAAT2221AAGGCAATAGCATCACAAATTTCACAAATAAGGCATTTTTTTCACTGCATTCTAGTTTTG2281GTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCTCAAATCCCTCGGAAGCTG2341CGCCTGTCTTAGGTTGGAGTGATACATTTTTATCACTTTTACCCGTCTTTGGATTAGGCA2401GTAGCTCTGACGGCCCTCCTGTCTTAGGTTAGTGAAAAATGTCACTCTCTTACCCGTCAT2461TGGCTGTCCAGCTTAGCTCGCAGGGGAGGTGGTCTGGATCCGCCGGCACCGGTGTATACG2521GGAATTCTTTACGAGGGTAGGAAGTGGTACGGAAAGTTGGTATAAGACAAAAGTGTTGTG2581GAATTGAAGTTTACTCAAAAAATCAGCACTCTTTTATAGGCGCCCTGGTTTACATAAGCA2641AAGCTTATACGTTCTCTATCACTGATAGGGAGTAAACTGGATATACGTTCTCTATCACTG2701ATAGGGAGTAAACTGTAGATACGTTCTCTATCACTGATAGGGAGTAAACTGGTCATACGT2761TCTCTATCACTGATAGGGAGTAAACTCCTTATACGTTCTCTATCACTGATAGGGAGTAAA2821GTCTGCATACGTTCTCTATCACTGATAGGGAGTAAACTCTTCATACGTTCTCTATCACTG2881ATAGGGAGTAAACTCGAGGTGATAATTCCACGGGGTTGGGGTTGCGCCTTTTCCAAGGCA2941GCCCTGGGTTTGCGCAGGGACGCGGCTGCTCTGGGCGTGGTTCCGGGAAACGCAGCGGCG3001CCGACCCTGGGTCTCGCACATTCTTCACGTCCGTTCGCAGCGTCACCCGGATCTTCGCCG3061CTACCCTTGTGGGCCCCCCGGCGACGCTTCCTGCTCCGCCCCTAAGTCGGGAAGGTTCCT3121TGCGGTTCGCGGCGTGCCGGACGTGACAAACGGAAGCCGCACGTCTCACTAGTACCCTCG3181CAGACGGACAGCGCCAGGGAGCAATGGCAGCGCGCCGACCGCGATGGGCTGTGGCCAATA3241GCGGCTGCTCAGCAGGGCGCGCCGAGAGCAGCGGCCGGGAAGGGGCGGTGCGGGAGGCGG3301GGTGTGGGGCGGTAGTGTGGGCCCTGTTCCTGCCCGCGCGGTGTTCCGCATTCTGCAAGC3361CTCCGGAGCGCACGTCGGCAGTCGGCTCCCTCGTTGACCGAATCACCGACCTCTCTCCCC3421AGGGGGATCATCGAATTACCATGTCTAGACTGGACAAGAGCAAAGTCATAAACTCTGCTC3481TGGAATTACTCAATGGAGTCGGTATCGAAGGCCTGACGACAAGGAAACTCGCTCAAAAGC3541TGGGAGTTGAGCAGCCTACCCTGTACTGGCACGTGAAGAACAAGCGGGCCCTGCTCGATG3601CCCTGCCAATCGAGATGCTGGACAGGCATCATACCCACTCCTGCCCCCTGGAAGGCGAGT3661CATGGCAAGACTTTCTGCGGAACAACGCCAAGTCATACCGCTGTGCTCTCCTCTCACATC3721G