蛋白质相关实验技术操作指南Word格式.doc

蛋白质相关实验技术操作指南Word格式.doc

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ThePreussLab,TheDivisionofBiologicalSciences,TheUniversityofChicago

WesternBlotofTBPfromTBP-GSTbacteria

StevenFinkbeiner,DepartmentsofNeurologyandPhysiology,UCSF

WesternBlot/Anti-P-CREB

Westernblottingprotocolfor1C2,3B5H10,and4C8Antibodies

Protocolforanti-HAantibodyWesternBlotting

ProbingandStrippingofWesternBlot

HahnLab，TheFredHutchinsonCancerResearchCenterandHowardHughesMedicalInstitute

WesternBlottingProtocol

MirmiraLaboratoryattheUniversityofVirginia

WesternBlottingandImmunostaining

ChazinLab,CenterforStructuralBiology,VanderbiltUniversity

WesternBlots

LabofDr.MarkBartonFrank,OklahomaMedicalResearchFoundation

WesternBlotting-Antibodies

TheMinionLab,CollegeofVeterinaryMedicineatIowaStateUniversity

WesternBlotting

Cepko/TabinLab,HarvardUniversity

CarbonateSolutionForWesternBlot

Dr.DEKoshland,CarnegieInstitutionofWashington

WesternBlotwithBSA

METHODforWesternBlots

MichaelKoelle'

sLab,DepartmentofMolecularBiophysicsandBiochemistry

YaleUniversity

2、蛋白分离/提取/浓缩

LysisBuffers

YeastProteinIsolation"

Yaffe-Schatz"

AmbergLab,UpstateMedicalUniversity

GuanidineHydrochloridePurificationofProteinsFromSDSPAGE

GottschlingLab，TheFredHutchinsonCancerResearchCenterandHowardHughesMedicalInstitute

YeastproteinprepforSDSPAGEandwestern（rapid）

YeastproteinprepforSDSPAGEandwestern（glassbead）

PurificationofTFIIICfromyeast

PurificationofTFIIAfromE.coli

TCAproteinprecipitation

PurificationofMLCK（MyosinLightChainKinase）fromRabbitSkeletalMuscle肌球蛋白轻链激酶

StullLab,UniversityofTexas,SouthwesternMedicalCenter

PurificationofCalmodulinfromBovineBrain

StullLab,UniversityofTexas,SouthwesternMedicalCenter

MyosinLightChainPreparationfromSkeletalandCardiacMuscle

PurifyingProteinfromInclusionBodies

ChazinLab,CenterforStructuralBiology,VanderbiltUniversity

ProtocolforProteinExtractionfromPlant

TheUniversityofNebraska-LincolnProteinCoreFacility（PCF）

TaqPolymerasePurification

YeastProteinPrep（NoBoil）

YeastProteinPrep（Boil）

GSTFusionProteinPrep

VesicleTrafficking,StanfordUniversity

3、蛋白质检测分析：

AMIDOBLACKSTAIN

HancockLaboratoryMethods,DepartmentofMicrobiologyandImmunology,

UniversityofBritishColumbia,BritishColumbia,Canada

JamesHardwick'

sangiotensinassayprotocol

SeftonLab,SalkInstitute

Phosphoaminoacidanalysis:

MarkKamps'

smethod

SeftonLab,SalkInstitute

ULTIMATEHIS-UBASSAYFOR

theTanseyLabatColdSpringHarborLaboratory

ULTIMATEHIS-UBASSAYFORYEAST

ProtocolforBeta-HexosaminidaseRBL-2H3SecretionAssay

meyerlab,StanfordUniversity

MeasurementofMyosinRegulatoryLightChain（RLC）Phosphorylation

BicinchoninicAcid（BCA）ProteinAssay

DavidR.Caprette,RiceUniversity

BiuretProteinAssay

Bradfordproteinassay

Hartree-LowryandModifiedLowryProteinAssays

ColorimetricAssays

Kdmeasurement,fitting,calculation,andsimulation

Chazi