木瓜的英语说范文Word文档下载推荐.docx

木瓜的英语说范文Word文档下载推荐.docx

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Papain[9001-73-4]

PapainisapurifiedproteolyticsubstancederivedfromCaricapapayaLinné

（Fam.caricaceae）.papain,whenassayeddirectedherein,containsnotlessthan6000unitspermg.Papainofahigherdigestivepowermaybereducedtotheofficialstandardbyadmixturewithpapainofloweractivity,lactose,orothersuitablediluents.

OneUSPUnitofpapainistheactivitythatreleasestheof1μgoftyrosinefromaspecifiedcaseinsubstanceundertheconditionsoftheAssay，usingtheenzymeconcentrationthatliberates40μgoftyrosinepermLoftestsolution.

Packagingandstorage－Preserveintight,light-resistantcontainersinacoolplace.

USPreferencestandards（11）—USPpapainRS.

pH:

between4.8and6.2inasolution（1in50）.

Lossondrying—Dryitinavacuumovenat60℃for4hours:

itlossesnotmorethan7.0%ofitsweight.

Assay（caseindigestivepower）—

Dibasicsodiumphosphate.,0.05M—dissolve7.1gofanhydrousdibasicsodiumphosphateinwatertomake1000mL.add1dropoftolueneasapreservative.

Citricacid,0.05M—dissolve10.5gofcitricacidmonohydrateinwatertomake1000mL.Add1dropontolueneasapreservative.

Caseinsubstrate—Disperse1gofHammersten-typecaseinin50mLon0.05MDibasicsodiumphosphate.Placeinaboilingwaterbathfor30minuteswithoasionalstirring.Cooltoroomtemperature,andadd0.05MCitricacidtoadjusttoapHof6.0±

0.1.stirthesolutionrapidlyandcontinuouslyduringtheadditionofthe0.05MCitricacidtopreventprecipitationofthecasein.Dilutewithwaterto100mL.preparefreshdaily.

Buffersolution（Phosphate-CysteineDisodiumethylenediamietraacetateBuffer）

Trichloroaceticacidsolution—Dissolve30gofreagentgradetrichloroaceticacidinwater.anddilutewithwaterto100mL.Thissolutionmaybestoredatroomtemperature.

Standardpreparation—Weighaurately100mgofUSPPapainRSina100-mLvolumetricflask.AndaddBuffersolutiontodissolve.DilutewithBuffersolutiontovolume,andmix.Transfer2.0mLofthissolutiontoa50-mLvolumetricflask,dilutewithBuffersolutiontovolume,andmix.Usewithin30minutesafterpreparation.

Assaypreparation—Transferanauratelyweighedamountofpapain.,equivalenttoabout100mgofUSPPapainRS,toa10-mLvolumetricflask,dilutewithBuffersolutiontovolume,andmix.Transfer2.0mLofthissolutiontoa50-mLvolumetricflsk,dilutewithBuffersolutiontovolume,andmix.

Procedure—Intoeachof12testtubes（18-×

150-mm）pipet5.0mLofcaseinsubstrate.Placeina

waterbathat40°

andallow10minutestoreachbathtemperature.Intoeachoftwoofthetubes（thetestsareruninduplicateexceptfortheblanks）labeledS1,pipet1.0mLoftheStandardpreparationand1.0mLoftheBuffersolution,Mixbyswirling,notezerotime,insertthestopper,andreplaceinthebath.Intoeachof2othertubes,labeledS2pipet1.5mLofstandard.preparationand0.5mLofBuffersolution,andproceedasbefore.Repeatthisprocedurefor2tubes,labeledS3towhich2.0mLofstandardpreparationisadded,andfor2tube,labledU2,towhich1.5mLofAssaypreparationand0.5mLBuffersolutionareadded.After60minutes,aurarytimed,addtoall12tubes3.0mLoftrichloroaceticacidsolution,andshakevigorously.Withthe4tubestowhichnostandardpreparationorAssaypreparationwereadded,prepareblanksbypipeting,respectively,1.0mLofstandardpreparationand1.0mLofBuffersolution

1.5mLofstandardpreparationand0.5mLofBuffersolution;

2.0mLofstandardpreparation;

and1.5mLAssaypreparationand0.5mLofBuffersolution.Replacealltubesinthe40°

waterbathfor30to40minutes,toallowtocoagulatefullytheprecipitatedprotein.Filterthroughmedium-porosityfilterpaper,discardingthefirst3mLofthefiltrate（filtratesusedareclear）.Readtheabsorbancesat280nm,ofthefiltratesifallsolutionsagainsttheirrespectiveblanks.PlottheabsorbancereadingsforS1,S2-andS3againsttheenzymeconcentrationofeachcorrespondinglevel.Byinterpolationfromthiscurve,takingintoconsiderationdilution

factors,calculatethepotencyinUnits,intheweightofpapaintakenbytheformula.:

（50,000/3）CA,

Inwhich50,000/3isafactorderivedbytheexpression100（50/2）（10/1.5）,Cistheconcentration,inmgpermL,obtainedfromthestandardcurve,andAistheactivityoftheReferenceStandardinUnitspermg.

翻译仅供参考：

木瓜蛋白酶

木瓜蛋白酶[9001-73-4]

木瓜蛋白酶是一种源自番木瓜乳汁（Fam.caricaceae）的纯化的水解蛋白物。

木瓜蛋白酶在这里定向检测时其含量不得少于6000单位每毫克。

具有较高的消化能力的木瓜蛋白酶也许可以通过掺加低活力的木瓜蛋白酶，乳糖或者其他合适的填充物来降低消化能力以达到官方的标准。

定义木瓜蛋白酶一个USP活力单位为在测定条件下指定的酪蛋白物质释放1μg酪氨酸所需要的量，所用的酶试液的浓度相当与每毫升释放40μg酪氨酸。

包装与保藏—包装要包紧，保存在避光，阴凉的地方

USP参考标准（11）—USP木瓜蛋白酶R.S

pH在溶液中介于4.8与6.2之间（1in50？

）

干燥失重—60℃时在真空干燥箱中干燥4