小鼠早期胚胎发育PPT资料.ppt

小鼠早期胚胎发育PPT资料.ppt

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小鼠早期胚胎发育,景乃禾中国科学院上海生物化学与细胞生物学研究所,体轴形成和原肠运动Axisformation&@#@Gastrulation,小鼠早期胚胎发育,Earlymousedevelopment,Preimplantation,Postimplantation,Earlymousedevelopment,（early）Epiblast,（late）Epiblast,Anteriorneuroectoderm,VE:

@#@visceralendodermAVE:

@#@anteriorVEDVE:

@#@distalVE,Celllineagesinearlymouseembryo,Morula,InnerCellMass,Trophectoderm,Primitiveendoderm,Epiblast,Parietalendoderm,Visceralendoderm,Definitiveendoderm,Mesoderm,Ectoderm,Mesendoderm,CNS,Epidermis,Mouseembryodevelopmentfromfertilizationtogastrulation,Development139,3-14,2012,？

@#@,？

@#@,？

@#@,Twopossiblemechanismsforthegenerationofmultiplecelltypesandpolarity,Development139,3-14,2012,DevCell16,398-410,2009,MousetrophectodermdistinguishfrominnercellmassregulatedbyHipposignalingpathway,Cellposition-dependentdifferentiationmediatedbyHipposignaling,Development139,3-14,2012,Mouseembryodevelopmentfromfertilizationtogastrulation,Development139,3-14,2012,？

@#@,Earlymousedevelopment,E3.5,E4.5,ICM,SaltandPepperexpressionofNanogandGata6intheICMofE3.5blastocysts,DevCell10,615-624,2006,SegregationandrelocationofepiblastandPrEcells,Development139,3-14,2012,Mouseembryodevelopmentfromfertilizationtogastrulation,Development139,3-14,2012,？

@#@,Emergingasymmetryandestablishmentofanterior-posteriorpolarityintheE5.75mouseembryo,AxisofAnterior-Posterior,AxisofProximal&@#@Distal,DistalVEtoAVE,Cellmovementsinearlymouseembryos,Epiblasttoposterior,Extraembryonicectodermtoposteriorepiblast,Epiblasttoposterior,MovementofOtx2-positivecellsfromDVEtoAVE,DVE:

@#@distalvisceralendoderm;@#@AVE:

@#@anteriorvisceralendoderm,AsymmetricexpressionofLefty1transgenesinperi-implantationmouseembryos,DevCell10,451-459,2006,TwotypesofLefty1+cellinmouseembryosbetweenE3.5andE4.5,NatCellBiol13,743-752,2011,DistinctoriginsofDVEandAVE,NatCellBiol13,743-752,2011,Originsofthedorsalandanteriorvisceralendoderm,Development139,3-14,2012,SignalingpathwaysinvolvedinA-Paxisdetermination,Development139,3-14,2012,Mouseembryodevelopmentfromfertilizationtogastrulation,Development139,3-14,2012,Nervoussystem,Skinandhair,Heart,Muscle,liver,Lungs,Earlymouseembryodevelopmentandlineagesegregation,3.5dpcICM,4.5dpcearlyepiblast,5.5dpclateepiblast,6.5dpc,Primitivestreak,Rapidproliferationduringgastrulation:

@#@dramaticsignalingandgeneexpressionchanges,Fossat,N.,etal.（2012）CellCycle11:

@#@26-32,“Itisnotbirth,marriage,ordeath,butgastrulation,whichistrulythemostimportanttimeinyourlife.”LewisWolpert,1986,VE:

@#@visceralendodermAVE:

@#@anteriorVEDVE:

@#@distalVE,Primitivestreakformation,PS:

@#@PrimitiveStreak,EMTatmouseprimitivestreak,EMTatmouseprimitivestreak,Epiblast,PrimitveStreak,NascentMesoderm,CellAdhesion&@#@Migration4:

@#@3,447-457,Mousegastrulationandgermlayerformation,Cell132,661680,2008,Myocardin,E7.5,E8.5,Latemousedevelopment,AxisofDorsal-Ventral,Celllineagesinearlymouseembryo,Embryoculturesystemfortime-lapsemovieacquisitionofnodemorphogenesis,DevCell13,884-896,2007,invitroembryoculture,GFPknock-intracing,Time-lapseimagesofnodemorphogenesis,Noto-,DevCell13,884-896,2007,Thedisplacementmodelofendodermmorphogenesisinthemousegastrula,Thedispersalmodelofendodermmorphogenesisinthemousegastrula,Visceralendoderm-derivedcellsareincorporatedintotheguttube,1218somiteembryosrevealedthepresenceofAfp-GFP+cellsintheguttube,Thiswasnotnewexpressionofthetransgene,asGFPmRNAcouldonlybedetectedintheyolksacatthisstage,Thesevisceralendodermderivedcellscontinuetodivideandareeventuallyincorporatedintotheepitheliumoftheguttube,DevCell15,509-520,2008,Celllineagesinearlymouseembryo,Definitiveendoderm,Mesoderm,Ectoderm,Mesendoderm,CNS,Epidermis,Epiblast,Celllineageinearlymouseembryo,Ectoderm,Mesendoderm,E7.5,Mesendodermmarkers,Geneexpressionandregionalizedallocationoftissueprogenitors,Axialmesoderm,Definitiveendoderm,Paraxialmesoderm,Lateralplatemesoderm,Extraembryonicmesoderm,Neuroectoderm,?

@#@,Themarkersofgermlayers,Definitiveendoderm,Mesoderm,Ectoderm,Mesendoderm,Neural,Epidermis,Nkx2.5,Sox17,Nodal,T,Wnt3,Gsc,Epiblast,Mesp1,Mixl1,Flk1,Gata4,Gata6,Foxa2,Sox1,Pax6,Nestin,Six3,Ck5,Ck8,Ck18,Zo-1,FGF5,Nodal,Fate-mappingofthedistalcapepiblastbycarbocyaninedyelabeling,E6.5,Dyeinjection,Thedistalcapepiblastcellsmigratetotheanteriorneuroectoderm,Development,107,55-67（1989）,CurrentBiology1996,Vol6No2,Fatemapsfortheectodermallayer,Wheatgermagglutinin（WGA）,Development139,423-436（2012）,Singlecellfatemapofectodermalcellsinearlymouseembryo,E7.0,singlecellinjectionofHRPandLRDX,andinvitroculture24hr,Development139,423-436（2012）,TissuecontributionsofdescendantcellsaccordingtotheoriginalpositionofHRP-injectedprogenitors,内胚层,中胚层,外胚层,中内胚层,神经外胚层,表面外胚层,上胚层,囊胚（内细胞团）,受精卵,多能干细胞的细胞谱系,Invivo,Invitr