老鼠和人的PDGF 引物.docx

老鼠和人的PDGF 引物.docx

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老鼠和人的PDGF引物

老鼠的PDGFA引物对应的CDS序列位置。

扩增长度：

319

（Senseprimer）5'GGCTCGAAGTCAGATCCAC3'

（Anti-senseprimer）5'CTTGACGCTGCTGGTGTTAC3'

1ATGAGGACCTGGGCTTGCCTGCTGCTCCTCGGCTGCGGATACCTCGCCCATGCCCTGGCC60

61GAGGAAGCCGAGATACCCCGGGAGTTGATCGAGCGGCTGGCTCGAAGTCAGATCCACAGC120

121ATCCGGGACCTCCAGCGACTCTTGGAGATAGACTCCGTAGGGGCTGAGGATGCCTTGGAG180

181ACAAGTCTGAGAGCCCATGGGTCCCATGCCATTAACCATGTGCCCGAGAAGCGGCCTGTG240

241CCCATTCGCAGGAAGAGAAGTATTGAGGAAGCCATTCCTGCAGTTTGCAAGACCAGGACG300

301GTCATTTACGAGATACCTCGGAGCCAGGTGGACCCCACATCGGCCAACTTCCTGATCTGG360

361CCCCCATGTGTGGAGGTGAAGCGCTGCACTGGCTGTTGTAACACCAGCAGCGTCAAGTGC420

421CAGCCTTCACGGGTCCACCACCGCAGTGTCAAGGTGGCCAAAGTGGAGTATGTCAGGAAG480

481AAGCCAAAATTGAAAGAGGTCCAGGTGAGGTTAGAGGAACACCTGGAGTGTGCATGTGCG540

541ACCTCCAACCTGAACCCAGACCATCGGGAGGAGGAGACAGGAAGGCGTAGGGAATCAGGT600

601AAAAAGCGGAAAAGGAAAAGGTTAAAACCCACCTAA

老鼠的PDGFB引物对应的CDS序列位置。

扩增长度314

（Senseprimer）5'GAGCTGGACTTGAACATGACC3'

（Anti-senseprimer）5'GGCTTCTTTCGCACAATCTC3'

1ATGAATCGCTGCTGGGCGCTCTTCCTTCCTCTCTGCTGCTACCTGCGTCTGGTCAGCGCC60

61GAGGGGGATCCCATTCCTGAGGAACTGTATGAAATGCTGAGCGACCACTCCATCCGCTCC120

121TTTGATGATCTTCAGCGCCTGCTGCACAGAGACTCCGTAGATGAAGATGGGGCTGAGCTG180

181GACTTGAACATGACCCGAGCACACTCCGGAGTCGAGTTGGAAAGCTCATCTCGAGGGAGG240

241AGGAGCCTAGGGTCCCTGGCAGCAGCAGAGCCTGCTGTAATCGCCGAGTGCAAGACGCGC300

301ACAGAGGTGTTCCAGATCTCTCGGAACCTCATCGATCGCACCAACGCCAACTTCCTGGTG360

361TGGCCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAATAACCGCAATGTGCAA420

421TGCCGGGCCTCGCAGGTGCAGATGCGGCCGGTCCAGGTGAGAAAGATTGAGATTGTGCGA480

481AAGAAGCCCATCTTCAAGAAGGCCACAGTGACCTTGGAGGACCACCTCGCCTGCAAGTGT540

541GAGACAGTAGTGACCCCTCGGCCTGTGACTAGAAGTCCTGGGACATCCAGGGAGCAGCGA600

601GCCAAGACGCCTCAAGCTCGGGTGACCATTCGGACGGTGAGAATCCGCCGGCCCCCCAAA660

661GGCAAGCACCGAAAGTTTAAGCACACCCATGACAAGGCGGCCCTGAAGGAGACCCTCGGA720

721GCCTAG726

老鼠的PDGFC引物对应的CDS序列位置。

扩增长度364

（Senseprimer）5'TGGACTTGGACAGCCTCTAC3'

（Anti-senseprimer）5'TGTTCCAGAGCCACATCAG3'

1ATGCTCCTCCTCGGCCTCCTCCTGCTGACATCTGCCCTGGCCGGCCAAAGAACGGGGACT60

61CGGGCTGAGTCCAACCTGAGCAGCAAGTTGCAGCTCTCCAGCGACAAGGAACAGAACGGA120

121GTGCAAGATCCCCGGCATGAGAGAGTTGTCACTATATCTGGTAATGGGAGCATCCACAGC180

181CCGAAGTTTCCTCATACATACCCAAGAAATATGGTGCTGGTGTGGAGATTAGTTGCAGTA240

241GATGAAAATGTGCGGATCCAGCTGACATTTGATGAGAGATTTGGGCTGGAAGATCCAGAA300

301GACGATATATGCAAGTATGATTTTGTAGAAGTTGAGGAGCCCAGTGATGGAAGTGTTTTA360

361GGACGCTGGTGTGGTTCTGGGACTGTGCCAGGAAAGCAGACTTCTAAAGGAAATCATATC420

421AGGATAAGATTTGTATCTGATGAGTATTTTCCATCTGAACCCGGATTCTGCATCCACTAC480

481AGTATTATCATGCCACAAGTCACAGAAACCACGAGTCCTTCGGTGTTGCCCCCTTCATCT540

541TTGTCATTGGACCTGCTCAACAATGCTGTGACTGCCTTCAGTACCTTGGAAGAGCTGATT600

601CGGTACCTAGAGCCAGATCGATGGCAGGTGGACTTGGACAGCCTCTACAAGCCAACATGG660

661CAGCTTTTGGGCAAGGCTTTCCTGTATGGGAAAAAAAGCAAAGTGGTGAATCTGAATCTC720

721CTCAAGGAAGAGGTAAAACTCTACAGCTGCACACCCCGGAACTTCTCAGTGTCCATACGG780

781GAAGAGCTAAAGAGGACAGATACCATATTCTGGCCAGGTTGTCTCCTGGTCAAGCGCTGT840

841GGAGGAAATTGTGCCTGTTGTCTCCATAATTGCAATGAATGTCAGTGTGTCCCACGTAAA900

901GTTACAAAAAAGTACCATGAGGTCCTTCAGTTGAGACCAAAAACTGGAGTCAAGGGATTG960

961CATAAGTCACTCACTGATGTGGCTCTGGAACACCACGAGGAATGTGACTGTGTGTGTAGA1020

1021GGAAACGCAGGAGGGTAA1038

老鼠的PDGFD引物对应的CDS序列位置。

扩增长度396

（Senseprimer）5'AAGCAGCCTCAGAGACCAAC3'

（Anti-senseprimer）5'AACCACAGTTGCCACCACAG3'

1ATGCAACGGCTCGTTTTAGTCTCCATTCTCCTGTGCGCGAACTTTAGCTGCTATCCGGAC60

61ACTTTTGCGACTCCGCAGAGAGCATCCATCAAAGCTTTGCGCAATGCCAACCTCAGGAGA120

121GATGAGAGCAATCACCTCACAGACTTGTACCAGAGAGAGGAGAACATTCAGGTGACAAGC180

181AATGGCCATGTGCAGAGTCCTCGCTTCCCGAACAGCTACCCAAGGAACCTGCTTCTGACA240

241TGGTGGCTCCGTTCCCAGGAGAAAACACGGATACAACTGTCCTTTGACCATCAATTCGGA300

301CTAGAGGAAGCAGAAAATGACATTTGTAGGTATGACTTTGTGGAAGTTGAAGAAGTCTCA360

361GAGAGCAGCACTGTTGTCAGAGGAAGATGGTGTGGCCACAAGGAGATCCCTCCAAGGATA420

421ACGTCAAGAACAAACCAGATTAAAATCACATTTAAGTCTGATGACTACTTTGTGGCAAAA480

481CCTGGATTCAAGATTTATTATTCATTTGTGGAAGATTTCCAACCGGAAGCAGCCTCAGAG540

541ACCAACTGGGAATCAGTCACAAGCTCTTTCTCTGGGGTGTCCTATCACTCTCCATCAATA600

601ACGGACCCCACTCTCACTGCTGATGCCCTGGACAAAACTGTCGCAGAATTCGATACCGTG660

661GAAGATCTACTTAAGCACTTCAATCCAGTGTCTTGGCAAGATGATCTGGAGAATTTGTAT720

721CTGGACACCCCTCATTATAGAGGCAGGTCATACCATGATCGGAAGTCCAAAGTGGACCTG780

781GACAGGCTCAATGATGATGTCAAGCGTTACAGTTGCACTCCCAGGAATCACTCTGTGAAC840

841CTCAGGGAGGAGCTGAAGCTGACCAATGCAGTCTTCTTCCCACGATGCCTCCTCGTGCAG900

901CGCTGTGGTGGCAACTGTGGTTGCGGAACTGTCAACTGGAAGTCCTGCACATGCAGCTCA960

961GGGAAGACAGTGAAGAAGTATCATGAGGTATTGAAGTTTGAGCCTGGACATTTCAAGAGA1020

1021AGGGGCAAAGCTAAGAATATGGCTCTTGTTGATATCCAGCTGGATCATCATGAGCGATGT1080

1081GACTGTATCTGCAGCTCAAGACCACCTCGATAA1113

老鼠的PDGFRA引物对应的CDS序列位置。

扩增长度322

（Senseprimer）5'TTGCCTTACGACTCCAGAT3'

（Anti-senseprimer）5'GCAAGTAGTTGACCAAATCC3'

1ATGGGGACCTCCCACCAGGTCTTTCTGGTCCTCAGCTGTCTCCTCACAGGGCCGGGCCTC60

61ATCTCCTGCCAGCTCTTATTACCCTCTATCCTCCCAAACGAGAATGAGAAGATTGTGCAG120

121CTGAATTCGTCCTTTTCTCTGAGATGCGTTGGGGAGAGTGAAGTGAGCTGGCAGCACCCC180

181ATGTCTGAAGAAGACGACCCCAACGTGGAAATCAGAAGTGAGGAGAACAACAGTGGCCTC240

241TTTGTCACAGTGCTGGAAGTGGTTAACGCCTCCGCAGCCCACACCGGATGGTACACTTGC300

301TACTACAACCACACTCAGACGGATGAGAGTGAGATCGAAGGCAGGCACATTTACATCTAT360

361GTACCAGACCCAGACATGGCCTTTGTGCCTCTCGGGATGACCGATTCCTTAGTCATCGTG420

421GAAGAGGATGACTCTGCCATCATACCTTGCCGCACCACAGATCCGGAGACTCAAGTAACC480

481TTGCACAATAACGGGAGGCTGGTGCCTGCCTCCTATGACAGCAGGCAGGGCTTCAACGGA540

541ACCTTCAGCGTGGGGCCTTACATCTGTGAGGCCACCGTCAAAGGGAGGACGTTCAAGACC600

601AGCGAGTTTAATGTTTATGCCTTGAAAGCAACGTCAGAACTGAATCTGGAGATGGACGCA660

661CGCCAGACTGTGTATAAGGCAGGAGAAACGATCGTGGTGACCTGTGCCGTCTTTAACAAC720

721GAGGTCGTTGACCTGCAGTGGACTTACCCTGGAGAAGTGAGAAACAAAGGTATCACCATG780

781CTGGAGGAGATCAAACTCCCATCCATCAAACTGGTGTACACTTTGACCGTCCCCAAGGCC840

841ACGGTGAAGGACAGTGGAGAGTATGAATGTGCTGCCCGTCAGGCCACTAAAGAGGTCAAG900

901GAAATGAAGAGAGTCACCATTTCTGTCCACGAGAAAGGCTTCGTCGAGATTGAGCCCACC960

961TTTGGCCAGCTGGAAGCTGTCAACTTGCACGAAGTCAGAGAGTTCGTGGTGGAGGTGCAG1020

1021GCCTACCCGACGCCCAGGATATCGTGGCTGAAGGACAACTTGACTCTGATAGAGAATCTC1080

1081ACCGAGATCACCACTGATGTACAGAAGAGTCAGGAAACAAGGTATCAGAGCAAATTAAAG1140

1141CTGATCCGGGCTAAGGAAGAAGACAGTGGCCATTACACCATTATAGTTCAAAATGAGGAT1200

1201GACGTGAAGAGTTACACGTTTGAGCTGTCAACCCTAGTTCCTGCATCCATTTTGGATCTC1260

1261GTAGATGACCACCATGGCTCTGGCGGGGGACAGACTGTGAGGTGTACAGCCGAAGGCACC1320

1321CCTCTTCCAGAAATCGACTGGATGATCTGCAAGCATATTAAGAAATGTAATAATGACACT1380

1381TCGTGGACAGTTTTGGCCAGCAATGTCTCAAATATTATCACAGAGCTCCCTCGCCGTGGC1440

1441AGGAGTACCGTGGAGGGACGAGTGTCCTTCGCCAAAGTGGAAGAGACCATCGCAGTTCGA1500

1501TGCCTGGCAAAGAACAACCTCAGCGTTGTGGCCCGTGAGCTGAAGCTGGTGGCTCCCACT1560

1561CTGCGATCTGAACTCACAGTGGCGGCCGCAGTGTTGGTGCTGTTGGTGATTGTCATTGTC1620

1621TCTCTCATCGTCCTGGTGGTCATTTGGAAGCAGAAACCACGGTATGAAATTCGCTGGAGG1680

1681GTTATCGAGTCAATCAGCCCCGATGGACATGAGTATATCTATGTGGACCCCATGCAGTTG1740

1741CCTTACGACTCCAGATGGGAATTCCCGAGAGATGGCCTCGTGCTTGGTCGGATTTTGGGA1800

1801TCCGGTGCATTTGGGAAAGTGGTCGAAGGTACAGCTTATGGATTAAGCCGGTCCCAACCT1860

1861GTAATGAAGGTGGCTGTGAAGATGCTCAAACCCACAGCCAGGTCTAGTGAAAAGCAGGCT1920

1921CTCATGTCTGAGCTGAAGATAATGACTCACCTGGGGCCACATTTGAACATTGTGAACTTG1980

1981CTGGGAGCCTGCACCAAGTCAGGTCCCATTTACATCATCACAGAATACTGCTTCTATGGG2040

2041GATTTGGTCAACTACTTGCATAAGAACAGAGATAGCTTCATGAGCCAACACCCAGAGAAG2100

2101CCGAAGAAAGACCTGGACATCTTTGGATTGAATCCTGCAGACGAGAGCACAAGAAGTTAT2160

2161GTGATTTTGTCTTTTGAAAATAACGGCGACTACATGGACATGAAGCAAGCTGATACCACA2220

2221CAGTATGTCCCCATGCTTGAAAGGAAAGAGGTTTCTAAATACTCTGACATCCAGAGATCG2280

2281CTGTACGATCGGCCAGCCTCCTACAAGAAGAAATCCATGCTAGACTCAGAAGTCAAAAAC2340

2341CTCCTTTCGGACGATGACTCCGAGGGTCTGACTTTGCTGGATCTATTGAGCTTCACCTAT2400

2401CAAGTTGCTCGAGGAATGGAATTTTTGGCTTCGAAAAATTGTGTCCACCGGGACCTGGCT2460

2461GCCCGCAACGTCCTCCTGGCGCAAGGAAAAATTGTGAAGATCTGTGATTTTGGCCTGGCC2520

2521AGAGACATCATGCACGATTCCAACTACGTGTCAAAAGGCAGTACTTTCCTGCCTGTGAAG2580

2581TGGATGGCACCCGAGAGCATCTTCGACAACCTCTACACCACGCTGAGTGACGTCTGGTCC2640

2641TATGGCATTCTGCTCTGGGAAATCTTTTCCCTTGGTGGCACACCCTATCCTGGCATGATG2700

2701GTCGATTCTACTTTCTACAATAAGATCAAGAGTGGATACCGGATGGCCAAACCTGACCAT2760

2761GCCACCAGTGAAGTCTATGAGATCATGGTGCAGTGCTGGAACAGTGAGCCCGAGAAGAGA2820

2821CCCTCCTTCTACCACCTCAGCGAGATAGTGGAGAACCTGTTGCCGGGACAATACAAGAAG2880

2881AGTTATGAAAAGATTCACCTGGACTTCCTAAAGAGTGACCATCCGGCCGTGGCACGCATG2940

2941CGGGTGGACTCTGATAATGCGTACATCGGTGTCACTTACAAAAATGAAGAGGATAAGCTG3000

3001AAGGACTGGGAAGGTGGCCTGGACGAACAGAGACTGAGCGCTGACAGTGGCTACATCATC3060

3061CCCCTGCCAGACATTGACCCTGTTCCAGAGGAGGAAGACCTGGGCAAGAGGAACAGACAC3120

3121AGCTCACAGACTTCGGAAGAGAGTGCCATCGAGACAGGTTCCAGTAGTTCCACCTTCATC3180

3181AAGAGAGAGGACGAGACCATCGAGGACATCGACATGATGGATGACATTGGTATAGATTCC3240

3241TCGGACCTGGTGGAGGACAGCTTCCTGTAA3270

老鼠的PDGFRB引物对应的CDS序列位置。

扩增长度469

（Senseprimer）5'GGCTACTATACTATGCGAGC3'

（Anti-senseprimer）5'TGACGGTAAGGACCACTAAG3'

1ATGGGGCTTCCAGGAGTGATACCAGCTTTAGTCCTCAGAGGCCAGTTGTTGCTGTCCGTG60

61TTATGGCTCCTGGGACCGCAGACCTCCCGGGGCCTAGTCATCACGCCCCCTGGGCCAGAG120

121TTTGTTCTCAACATCTCCAGCACCTTTGTTCTGACCTGCTCGGGCTCAGCTCCGGTGATG180

181TGGGAACAGATGTCCCAGGTGCCCTGGCAAGAAGCGGCCATGAATCAGGACGGCACCTTC240

241TCCAGTGTGCTGACACTGACCAATGTCACTGGGGGAGACACTGGGGAATACTTTTGTGTC300

301TACAACAACTCACTAGGGCCGGAGCTCAGTGAGAGGAAGCGTATCTATATCTTTGTGCCA360

361GATCCCACCATGGGCTTCCTCCCTATGGACTCCGAGGACCTGTTCATTTTTGTCACGGAT420

421GTCACTGAGACGACAATTCCGTGCCGAGTGACAGACCCCCAGCTGGAGGTGACGCTACAT480

481GAGAAGAAAGTGGATATCCCCCTACACGTACCCTACGACCACCAGCGAGGTTTCACTGGT540

541ACTTTTGAGGACAAGACTTACATTTGCAAAACCACCATTGGGGACAGGGAAGTGGACTCC600

601GATACTTACTACGTCTACAGCCTCCAGGTGTCATCCATCAACGTCTCTGTGAATGCCGTG660

661CAGACTGTGGTCCGCCAGGGCGAGAGCATCACCATCCGGTGCATTGTGATGGGCAATGAT720

721GTGGTGAACTTCCAATGGACGTACCCCCGCATGAAGAGTGGGCGGCTGGTGGAGCCAGTG780

781ACAGACTACCTCTTTGGAGTGCCCTCCCGCATTGGCTCCATCCTGCATATCCCCACGGCT840

841GAGCTGAGTGATTCGGGCACCTATACTTGCAACGTGTCAGTGAGTGTGAACGACCATGGC900

901GATGAGAAAGCCATCAACATCTCTGTGATCGAGAATGGCTA