常用载体及通用测序引物.docx

1、常用载体及通用测序引物VectorPrimer(F)Primer(R)抗性载体大小(bp)EZ-TM13-47RV-MAmp+2984LZRS-RfALZRS-FLZRS-R11100p3xFLAG CMV14CMV30(834bp)CMV24(1151bp)6310p3XFlag-myc-CMV-24CMV-F(852bp)/PCEP-F(896bp)R(hGHpolyA)(1217bp)4752pAAV-MCSF(-globin)(1192bp)R(hGHpolyA)(1521bp)4650pAc5.1/V5-His AAc5-Profor(2525bp)BGH(2768bp)/EBVre

2、v(2844bp)Amp+5375pAc5.1/V5-His BAc5-Profor(2525bp)BGH(2772bp)/EBVrev(2848bp)Amp+5379pAc5.1/V5-His CAc5-Profor(2525bp)BGH(2764bp)/EBVrev(2840bp)Amp+5371pAcGP67_APH-Profor(4016bp)pAcGP67rv(4329bp)Amp+9761pAcGP67_BPH-Profor(4016bp)pAcGP67rv(4329bp)Amp+9761pAcGP67_CPH-Profor(4016bp)pAcGP67rv(4329bp)Amp+

3、9761pACTT7(1061bp)T3(1420bp)Amp+5566pACT25AD(同GAL4AD)(5134bp)3AD(4939bp)/pGAL4-Adrv(4964bp)(pGAL4-Adrv离MCS少于20bp，优先用3AD)Amp+8117PACYCDuet-1(MCS)DuetUP1(3949bp)DuetDOWN1(209bp)Chl+4008PACYCDuet-1(MCS)DuetUP2(189bp)T7ter(468bp)Chl+4008pAdTrackpEGFP-N-3(同EGFP-Nrev)(1294bp)CMV-F(1433bp)Amp+/Kan+8111pAdT

4、rack-CMVpEGFP-N-3(同EGFP-Nrev)(1272bp)EBVrev(1982bp)Kan+9010PAO8155AOX3AOX7700pAS2-15BD(同GAL4BD)(5894bp)3BD(6112bp)/pGAL4-BDrv(6046bp)(优先用3BD)Amp+8392pB42ADpB42ADF(787bp)pB42ADR(924bp)Amp+6449PBABE-IRES-EgfppLXSN-F(同pLXSNfw)(1027bp)/PBABE5(1063bp)/pBMN5(1416bp)无反向引物5944pBabe-puropBabe5(1318bp)pBabe3(

5、1429bp)5169pBACPAK8BAC1(1181bp)/PH-Profor(1174bp)/BaculoVfor(1211bp)BAC2(1374bp)/BaculoVrev(1375bp)Amp+5538pBAD/His ApBADfw(211bp)/Xpressfor(375bp)(优先用pBADfw)pBADrv(514bp)4102pBBR1MCSM13F(3161bp)/T7(3188bp)/M13-47(3135bp)M13R(3390bp)/T3(3352bp)/RV-M(3409bp)Amp+4707pBI12135S(5735bp)无14758pBK-CMVT7(97

6、5bp)/M13F(951bp)T3(1159bp)/M13R(1196bp)Amp+4518pBluescript II KS (-)M13F(599bp)/T7(626bp)T3(792bp)/M13R(829bp)2961pBluescript II KS (+)M13F(599bp)/T7(626bp)T3(792bp)/M13R(829bp)2961pBluescript II SK (-)M13F(599bp)/T7(626bp)T3(792bp)/M13R(829bp)2961pBlueScript II SK(+)M13F(599bp)/T7(626bp)T3(792bp)/M

7、13R(829bp)2961pBluescript KS (-)M13F(602bp)/T7(626bp)T3(790bp)/M13R(828bp)2958pBluescript KS (+)M13F(602bp)/T7(626bp)T3(790bp)/M13R(828bp)2958pBluescript SK (-)M13F(602bp)/T7(626bp)T3(790bp)/M13R(828bp)2958pBluescript SK (+)M13F(602bp)/T7(626bp)T3(790bp)/M13R(828bp)2958pBSN-TT7T3Amp+pBudCE4.1(MCSI)C

8、MV-Profor(552)/T7(638)c-mycrev(742bp)/EBVrev(842bp)4595pBudCE4.2(MCS)PEF-F(2999)BGH(3235)4595pBV220PBV220F(3592bp)PBV220R(93bp)3667pCAMBIA1300P1 (11472bp)P2 (2728bp)11566pCAMBIA1301P1 (10935bp)P2 (11149bp)11849pCAMBIA1304P1(11454bp)P2(11668bp)12361pCAMBIA2300M13F(8418bp)/M13R(-48) (8313bp)M13F(-47)(

9、8461bp)/ M13R(8349bp)8742pCANTAB_5EpCANTAB5-S1(2265bp)/pCANTAB5-R1(2222bp)pCANTAB5-S6(2471bp)/pCANTAB5-R2(2504bp)4517pCAT3-enhancerRVP3(4215bp)无Amp+4273pCDFDuetT7(214bp)T7ter(467bp)3781pCDH-CMV-MCS-EF1-PuroCMV-F(2164bp)/PCEP-F(2208bp)/pLNCX-F(2210bp)无7384pcDNA1.1CMV-F(2076bp)/T7(2170bp)无4000pcDNA2.1

10、M13F/T7M13R/SP63013pcDNA3.0pcDNA3fw(841bp)/CMV-Profor(777bp)/T7(863bp)SP6(1016bp)/BGH(1053bp)5446pcDNA3.1 (-)T7(863bp)/CMV-Profor(777bp)/pcDNA3fw(841bp)BGH(1039bp)5428pcDNA3.1 (+)T7(863bp)/CMV-Profor(777bp)/pcDNA3fw(841bp)BGH(1039bp)5428pcDNA3.1/His AT7(863bp)/CMV-Profor(777bp)BGH(1122bp)Amp+5514pcD

11、NA3.1/His BT7(863bp)/CMV-Profor(777bp)BGH(1122bp)Amp+5514pcDNA3.1/His CT7(863bp)/CMV-Profor(777bp)BGH(1122bp)Amp+5514pcDNA3.1/myc-His AT7(863bp)/CMV-Profor(777bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5494pcDNA3.1/myc-His BT7(863bp)/CMV-Profor(777bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5494pcDNA3.1/myc-His CT7(

12、863bp)/CMV-Profor(777bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5494pcDNA3.1/V5-His AT7(863bp)/CMV-Profor(777bp)BGH(1108bp)/V5rev(1035bp)Amp+5502pcDNA3.1/V5-His BT7(863bp)/CMV-Profor(777bp)BGH(1108bp)/V5rev(1035bp)Amp+5502pcDNA3.1/V5-His CT7(863bp)/CMV-Profor(777bp)BGH(1108bp)/V5rev(1035bp)Amp+5502pcDNA3.1/

13、Zeo(-)T7(863bp)/CMV-F(769bp)BGH(1038bp)Amp+5014pcDNA3.1/Zeo(+)T7(863bp)/CMV-F(769bp)BGH(1039bp)Amp+5015pcDNA3.3/TOPOTACMV-F(584bp)/PCEP-F(628bp)/pLNCX-F(630bp)无Amp+5407pcDNA4/HisMax AXpressfor(1119bp)BGH(1282bp)Amp+5258pcDNA4/HisMax BXpressfor(1119bp)BGH(1282bp)Amp+5258pcDNA4/HisMax CXpressfor(1119b

14、p)BGH(1282bp)Amp+5258pcDNA4/HisMax-TOPOXpressfor(1119bp)BGH(1302bp)Amp+5274pcDNA4/HisMax-TOPO/lacZXpressfor(1119bp)BGH(4482bp)Amp+8354pcDNA4/myc-His ACMV-Profor(777bp)/T7(863bp)BGH(1103bp)/ c-mycrev(1024bp)Amp+5079pcDNA4/myc-His BCMV-Profor(777bp)/T7(863bp)BGH(1103bp)/ c-mycrev(1024bp)Amp+5079pcDNA4

15、/myc-His CCMV-Profor(777bp)/T7(863bp)BGH(1103bp)/ c-mycrev(1024bp)Amp+5079pcDNA4/TOTREfor(908bp)/CMV-Profor(777bp)BGH(1106bp)Amp+5078pcDNA4/V5-His ACMV-Profor(777bp)/T7(863bp)BGH(1117bp)/V5rev(1044bp)Amp+5092pcDNA4/V5-His BCMV-Profor(777bp)/T7(863bp)BGH(1117bp)/V5rev(1044bp)Amp+5092pcDNA4/V5-His CCM

16、V-Profor(777bp)/T7(863bp)BGH(1117bp)/V5rev(1044bp)Amp+5092pcDNA5/FRTCMV-Profor(777bp)/T7(863bp)BGH(1039bp)Amp+5069pcDNA6/myc-His ACMV-Profor(777bp)/T7(863bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5126pcDNA6/myc-His BCMV-Profor(777bp)/T7(863bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5126pcDNA6/myc-His CCMV-Profor(7

17、77bp)/T7(863bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5126pcDNA6/V5-His ACMV-Profor(777bp)/T7(863bp)BGH(1121bp)/V5rev(1048bp)Amp+5148pcDNA6/V5-His BCMV-Profor(777bp)/T7(863bp)BGH(1121bp)/V5rev(1048bp)Amp+5148pcDNA6/V5-His CCMV-Profor(777bp)/T7(863bp)BGH(1121bp)/V5rev(1048bp)Amp+5148pcDNAIIT7(2997bp)SP6(152

18、bp)3013pcDNA6.2-GW/EmGFP-miRmiRNA-FmiRNA-RAmp+5699pCEP4EBVrev(367bp)pCEP-F(500bp)Amp+10410pCI(neo)17Base(996bp)/T7(1067bp)(17Base在T7之前) pCI-neoF(886bp)EBVrev(1206bp)/T3(1158bp)Amp+5472pCMS-EGFPT7(1067bp)T3(1158bp)Amp+5541pCMV-3Tag-4AT3(621bp)/PFLAG-CMV-F(536bp)T7(906bp)/BGH(952bp)4233pCMV5CMV-F(853b

19、p)/PCEP-F(897bp)无Amp+4657pCMV5-FlagpCMV5FpCMV5RAmp+PCMV6V1.5(815bp)/CMV-Profor(861bp)XL39(1077bp)/HGHrev(1022bp)Amp+4665PCMV6-XL4CMV-F(882bp)/T7(952bp)/SP6(952bp)M13R(1048bp)/HGHrev(1089bp)Amp+4707PCMV-ACV1.5XL39(同CMV-24)5939pCMV-HACMV-Profor(485bp)/TREfor(570bp)EBVrev(965bp)Amp+3782pCMV-MCSF(-globi

20、n)R(hGHpolyA)4492pCMV-MYCpCMV-F(794bp)/CMV-Profor(490bp)/TREfor(570bp)pCMV-R(965bp)/EBVrev(973bp)Amp+3791pCMV-Sport1T7(646bp)SP6(806bp)/pLNCX-F(924)/CMV-F(970bp)(优先用SP6)Amp+4380pCMV-Sport6M13F(663bp)/T7(691bp)SP6(872bp)/M13R(899bp)4397pCMV-Tag(KAN+)T3(621bp)T7(840bp)Amp+4219pCMV-Tag1(KAN+)T3(621bp)T

21、7(840bp)4219pCMV-Tag2(KAN+)T3(621bp)T7(840bp)4219pCMV-Tag-2BT3(621bp)T7(845bp)4323pCMV-Tag3(KAN+)T3(621bp)T7(847bp)4219pCMV-Tag-3BT3(621bp)T7(846bp)4300pCMV-Tag4(KAN+)T3(621bp)T7(840bp)4219pCMV-Tag5(KAN+)T3(621bp)T7(843bp)4219PCMX-GAL4(MCS区)CMV-FCMV-R4500pCOLADuet-1(MCS2)T7(214bp)/DuetUP2(189bp)(优先用

22、T7)T7ter(467bp)Kan+3719pcoldpcoldI-F(208bp)pcoldI-R(387bp)4392pcoldpcoldI-F(208bp)pcoldI-R(374bp)4377pcoldpcoldI-F(208bp)pcoldI-R(356bp)4377pcoldIpcoldI-F(208bp)pcoldI-R(404bp)4407pcoldIpcoldI-F(208bp)pcoldI-R(404bp)4407pcoldIIIpcoldI-FpcoldI-R4751pCold-SUMOpCold-SUMO-FpCold-SUMO-RAmp+4751pCold-TFpC

23、old-TF-F1pCold-SUMO-RAmp+5769pCR blunt II TOPOSP6(239bp)/M13R(205bp)/RV-M(186bp)T7(425bp)/M13F(449bp)/M13-47(475bp)Amp+/Kan+3512pCR2.1M13R(205bp)M13F(406bp，)/T7(382bp)3907pCR2.1-TOPOM13R(205bp)M13F(409bp)/T7(385bp)(T7离MCS只有6bp，优先用M13F)Amp+/Kan+3909pCR3.1T7(638bp)BGH(831bp)Amp+/Kan+5060pCR3.1-UniT7(6

24、38bp)BGH(790bp)5020pCR4-TOPOT3(243bp)/M13R(205bp)/RV-M(186bp)T7(374bp)/M13F(371bp)/M13-47(397bp)Amp+/Kan+3956PCRE-LUCRVP3(4976bp)GLP2(327bp)5034pCR-ScriptSKT7/M13FT3/M13RAmp+/Kan+pCS2+ (MCSI)SP6(35bp)EBVrev(188bp)Amp+4095pCS2+ (MCSII)pSG5rv(222bp)T3(409bp)Amp+4095pDBLEUpDBLeu-F(6137bp)pDBLeu-R(6395b

25、p)9903pDEST15 GatewayT7(25bp)/PGEX-F(721bp)/pGEXrv(5270bp)T7ter(2579bp)Amp+7013PDEST20PH-Profor(43bp)/PGEX-F(777bp)/EBVrev(2771bp)无Amp+7066PDEST22PDEST22-F(4610bp)/pDEST22-F1998(4540bp)PDEST22-R(6539bp)/pDEST22-R4000(6627bp)Amp+8923pDNR-LIB(MCSA)M13F(4065bp)/T7(4089bp)M13R(385bp)4200pDNR-LIB(MCSB)EB

26、Vrev(253bp)M13R(385bp)4200pDONR/ZeoM13F(536bp)M13R(3044bp)4291pDONR201SeqL-A(286bp)SeqL-B(2789bp)4470pDONR207SeqL-A(5567bp)SeqL-B(2485bp)5585pDONR221M13F(536bp)M13R(3044bp)4762pDriveT7(239bp)/M13R(204bp)SP6(418bp)/M13F(449bp)Amp+/Kan+3851pDsRED1-C1pDsRED-ex-C1-F(1222bp)无4686pDSRED1-N1CMV-Profor(519b

27、p)RFP-Nrev(751bp)4692pDsRED2-C1pDsRED-ex-C1-F(1219bp)无反向引物4675pDSRED-N1pEGFP-N-5pDSRED-N1-RKan+pEarlygate10235S(2580bp)12453PEarlygate20135S(2580bp)11778pEasy-bluntM13R(205bp)M13F(406bp)/T7(382bp)3929pEasy-E1T7(209bp)T7ter(440bp)(离MCS不到20bp)5715pEasy-E2T7(5117bp)T7ter(5327bp)5393pEasy-T1M13R(205bp)/RV-M(186bp)M13F(405bp)/M13-47(431bp)(优先用RV-M和M13-47)3928pEasy-T1 SimpleM13R(205bp)/RV-M(186bp)M13F(306bp)/M13-47(332bp)(优先用RV-M和M13-47)3829pEasy-T3M13F(2996bp)/T7(3036bp删几个碱基后才能找到，最好不用)M13R(214bp)/SP6(180bp)(优先用M13引物)3036pECFP-C1EGFP-Cfor(1219bp)SV40-pArev(1576bp)4731pECFP-N1C