NEB 同尾酶表.docx

1、NEB 同尾酶表连接互相匹配的粘性末端，产生新的酶切位点通常连接互相匹配的粘性末端可以产生新的酶切位点。以下组合是通过计算机程序计算出来的，尽管我们希望能尽量保证其准确性，但有些未经实验证实，因此不能保证100%的可行性。如果有同裂酶存在，则只列出其中的一个酶。本表中列出的切酶均为已商品化的切酶。识别序列不够严谨的切酶（例如，识别多个序列的酶）只在括号标出相关的序列，但注意这些酶不只切割该序列。-表示连接产物不能被再切割。酶连接到可进行切割的酶Acc65 I (G/GTACC)Ban I (G/GTACC)Acc65 I, Ban I, Kpn I, Nla IV, Rsa IBsiW I,

2、BsrG IRsa IAcc I(GT/CGAC)Aci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I-(GT/CGAC)Cla I, BstB I, Taq ITaq IAci I(C/CGC) Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I-BsaH I (GR/CGCC), HinP1 I, Nar IAci IHpa IIHpa IIAcl I (AA/CGTT)Acc I (GT/CGAC), Aci I, Cla I, BstB I, HinP1 I, Hpa II, Nar I, T

3、aq I-Afl III(A/CGCGT)Asc I, BssH IIBstU I, Hha I(A/CATGT)BspH I, Nco I, Pci INla III(A/CGCGT)Mlu IAfl III, BstU I, Mlu IAge I (A/CCGGT)Ava I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBsaW I, BspE IBsaW I, Hpa IIBsrF I (A/CCGGT), SgrA I (CA/CCGGTG)Age I, BsaW I, BsrF I, Hpa IINgoM IVBsrF I, Hpa IIApa I (G

4、GGCC/C)Ban II (GGGCC/C), Bsp1286 I (GGGCC/C)Apa I, Ban II, Bsp120 I, Bsp1286 I, Hae III, Nla IV, Sau96 IApaL I (G/TGCAC)Sfc I (C/TGCAG)Bsg IApo I(A/AATTY)EcoR IApo I, Tsp509 I(G/AATTY)EcoR IApo I, EcoR I, Tsp509 I(R/AATTY)Mfe I, Tsp509 ITsp509 IAsc I (GG/CGCGCC)Afl III (A/CGCGT), Mlu IBstU I, Hha IB

5、ssH IIBssH II, BstU I, Cac8 I, Hha IAse I (AT/TAAT)Bfa I, Csp6 I, Nde I-Mse IMse IAsiS I(GCGAT/CGC) BsiE I (CGAT/CG)Dpn II, Pvu IPac IMse IPvu IDpn II, Pvu IAva I(C/CCGGG)Age I, BsaW I, BspE I, BsrF I (R/CCGGY), NgoM IV, SgrA I (CR/CCGGYG)Hpa II, Nci I, ScrF I(C/TCGAG)Xho IAva I, Taq I, Xho I(C/TCGA

6、G)Sal ITaq I(C/CCGGG)Xma IAva I, BsaJ I, Hpa II, Nci I, ScrF I, Sma IAva II (G/GWCC)PpuM I (RG/GACCY)Ava II, Nla IV, Sau96 IRsr IIAva II, Sau96 IPpuM I (RG/GTCCY)Ava II, BsmF I, Nla IV, Sau96 IAvr II (C/CTAGG)Nhe I, Spe I, Xba IBfa ISty I (C/CTAGG)Avr II, Bfa I, BsaJ I, Sty IBamH I (G/GATCC)Bcl I, D

7、pn IIAlw I, Dpn IIBgl II, BstY I (R/GATCY)Alw I, BstY I, Dpn IIBstY I (G/GATCC)Alw I, BamH I, BstY I, Dpn II, Nla IVBan I(G/GTACC)Acc65 IAcc65 I, Ban I, Kpn I, Nla IV, Rsa I(G/GCGCC)Kas IBan I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IV(G/GTACC)BsiW I, BsrG IRsa IBan II(GGGCC/C)Apa I, Bsp1286 I (GG

8、GCC/C)Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I(GAGCT/C)Bsp1286 I (GAGCT/C), Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac IBcl I(T/GATCA) BamH I, BstY I (R/GATCY)Alw I, Dpn IIBgl II, Mbo IIDpn IIBfa I (C/TAG)Ase I, Csp6 I, Mse I, Nde I-Bgl II (A/GATCT)BamH I, BstY I (R/GATCY)Alw I, BstY I,

9、Dpn IIBcl I, Dpn IIDpn IIBsaH I(GR/CGYC)Acc I (GT/CGAC), Cla I, BstB I, Taq I-(GA/CGYC)Aci I, HinP1 IHga I(GG/CGYC)Aci I, HinP1 IHha I(GG/CGYC)Hpa IIAci I(GA/CGYC)Nar IBsaH I, Hga I(GG/CGYC)Nar IBan I, BsaH I, Hae II, Hha I, Nar I, Nla IVBsaW I (W/CCGGW)Age I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)Age

10、 I, BsaW I, BsrF I, Hpa IIAva I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBspE IBsaW I, BspE I, Hpa IIBsrF I (R/CCGGY), NgoM IVBsrF I, Hpa IINgoM IVHpa IIBsiE I(CGAT/CG)Pac IMse I(CGAT/CG)Pvu IBsiE I, Dpn II, Pvu I(CGGC/CG)Sac IIAci IBsiHKA I(GTGCA/C) Bsp1286 I (GTGCA/C)BsiHKA I, Bsp1286 IBsp1286 I (GAGC

11、A/C)BsiHKA I, Bsp1286 IBsp1286 I (GAGCT/C), Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac INsi I-Pst I, Sbf IBsg IBsiW I (C/GTACG)Acc65 I, Ban I (G/GTACC), BsrG IRsa IBsp1286 I(GGGCC/C)Apa I, Ban II (GGGCC/C)Apa I, Ban II, Bsp1286 I, Hae III, Nla IV, Sau96 I(GTGCA/C)BsiHKA IApaL I, BsiHKA I, Bsp1286

12、I(GGGCC/C)Ban II (GGGCC/C)Ban II, Bsp1286 I(GAGCT/C)Ban II (GAGCT/C), BsiHKA I, Sac IAlu I, Ban II, BsiHKA I, Bsp1286 I, Sac I(GWGCW/C)BsiHKA IBsiHKA I, Bsp1286 I(GTGCA/C)Nsi I-(GTGCA/C)Pst I, Sbf IBsg IBspE I (T/CCGGA)Age I, BsaW I, BsrF I (R/CCGGY), SgrA I (CR/CCGGYG)BsaW I, Hpa IIAva I (C/CCGGG),

13、 Xma IHpa II, Nci I, ScrF IBsaW IBsaW I, BspE I, Hpa IIBsrF I (R/CCGGY), NgoM IVHpa IIBspH I (T/CATGA)Afl III (A/CATGT), Nco I, Sty I (C/CATGG)Nla IIIBsrF I(A/CCGGY)Age I, BsaW IAge I, BsaW I, BsrF I, Hpa II(G/CCGGY)Age I, BsaW I, NgoM IVBsrF I, Hpa II(R/CCGGY)Ava I (C/CCGGG), Xma IHpa II, Nci I, Sc

14、rF I(A/CCGGY)BsaW I, BspE IBsaW I, Hpa II(R/CCGGY)BsaW I, BspE IHpa II(G/CCGGY)NgoM IVBsrF I, Cac8 I, Hpa II, Nae I(CR/CCGGYG)SgrA IBsrF I, Hpa IIBsrG I (T/GTACA)Acc65 I, Ban I (G/GTACC), BsiW IRsa IBssH II (G/CGCGC)Afl III (A/CGCGT), Mlu IBstU I, Hha IAsc IBssH II, BstU I, Cac8 I, Hha IBstB I (TT/C

15、GAA)Acc I (GT/CGAC), Cla I, Taq ITaq IAci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I-BstY I(A/GATCY)BamH I, Bgl IIAlw I, BstY I, Dpn II(G/GATCY)BamH IAlw I, BamH I, BstY I, Dpn II, Nla IV(R/GATCY)Bcl I, Dpn IIDpn II(G/GATCY)Bcl I, Dpn IIAlw I, Dpn II(A/GATCY)Bgl IIBgl II, BstY I, Dpn IICla I

16、 (AT/CGAT)Acc I (GT/CGAC), BstB I, Taq ITaq IAci I, Acl I, BsaH I (GR/CGYC), HinP1 I, Hpa II, Nar I-Dpn II/Mbo I/ Sau3A I (/GATC)BamH I, BstY I (R/GATCC)Alw I, Dpn IIBcl I, Bgl II, BstY I (R/GATCY)Dpn IIEae I(Y/GGCCR)PspOM IHae III, Sau96 I(C/GGCCR)Eag IBsiE I, Eae I, Eag I, Hae III(T/GGCCR)Eag IEae

17、 I, Hae III(C/GGCCR)Not IAci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae III(T/GGCCR)Not IAci I, Eae I, Fnu4H I, Hae IIIEag I (C/GGCCG)PspOM IHae III, Sau96 IEae I (Y/GGCCR)Eae I, Hae IIIEae I (C/GGCCG)BsiE I, Eae I, Eag I, Hae IIINot IAci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae IIIEcoR I (G/AATTC)Apo I (G/

18、AATTC)Apo I, EcoR I, Tsp509 IApo I (R/AATTY)Apo I, Tsp509 IMfe I, Tsp509 ITsp509 IHinP1 I (G/CGC)Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I-Aci I, BsaH I (GR/CGCC), Nar IHha IBsaH I (GR/CGTC)Hga IHpa IIAci IHpa II/Msp I (C/CGG)Acc I (GT/CGAC), Acl I, Cla I, BstB I, Taq I-Aci I, BsaH I (GR/CGCC), H

19、inP1 I, Nar IAci IKas I (G/GCGCC)Ban I (G/GCGCC)Ban I, BsaH I, Hae II, Hha I, Kas I, Nar I, Nla IVMfe I (C/AATTG)Apo I (R/ATTTY), EcoR I, Tsp509 ITsp509 IMlu I (A/CGCGT)Afl III (A/CGCGT)Afl III, BstU I, Mlu IAsc I, BssH IIBstU I, Hha IMse I (T/TAA)Ase IMse IBfa I, Csp6 I, Nde I-Nar I (GG/CGCC)Acc I

20、(GT/CGAC), Acl I, Cla I, BstB I, Taq I-Aci I, HinP1 IHha IBsaH I (GR/CGCC)Ban I, BsaH I, Hae II, Hha I, Nar I, Nla IVBsaH I (GR/CGTC)BsaH I, Hga IHpa IIAci INco I (C/CATGG)Afl III (A/CATGT), BspH INla IIISty I (C/CATGG)BsaJ I, Nco I, Nla III, Sty IPci INla IIINde I (CA/TATG)Ase I, Bfa I, Csp6 I, Mse

21、 I-NgoM IV (G/CCGGC)Age I, BsaW I, BsrF I (R/CCGGY), SgrA IBsrF I, Hpa IIAva I (C/CCGGG), Xma IHpa II, Nci I, ScrF IBsaW I, BspE IHpa IIBsrF I (R/CCGGC), SgrA IBsrF I, Cac8 I, Hpa II, Nae INhe I (G/CTAGC)Avr II, Spe I, Sty I (C/CTAGG), Xba IBfa INla III (CATG/)Sph I, Nsp INla IIINot I (GC/GGCCGC)Psp

22、OM IAci I, Eae I, Fnu4H I, Hae IIIEag IAci I, BsiE I, Eae I, Eag I, Fnu4H I, Hae IIIEae I (Y/GGCCR)Aci I, BsiE I, Eae I, Fnu4H I, Hae IIINsi I (ATGCA/T)BsiHKA I (GTGCA/C), Bsp1286 I (GTGCA/C), Pst I, Sbf I-Nsp I (RCATG/Y)Nla III, Sph INla IIIPac I (TTAAT/TAA)BsiE I (CGAT/CG), Pvu IMse IPci I (A/CATG

23、T)Afl II (A/CATGT), BspH I, Nco I, Sty I (C/CTAGG)Nla IIIPpuM I(RG/GWCCY)Ava II, Rsr IIAva II, Sau96 I(GG/GTCCY)Ava II, Rsr IIAva II, BsmF I, Nla IV, Sau96 I(GG/GACCY)Ava II, Rsr IIAva II, Nla IV, Sau96 IPspOM I (G/GGCCC)Eae I (Y/GGCCR), Eag IHae III, Sau96 INot IAci I, Fnu4H I, Hae III, Sau96 IPst

24、I (CTGCA/G)BsiHKA I, Bsp1286 I (GTGCA/C)Bsg INsi I-Sbf IPst IPvu I (CGAT/CG)AsiS IDpn I, Pvu IPac IMse IBsiE I (CGAT/CG)BsiE I, Dpn II, Pvu IRsr II (CG/GWCCG)Ava II, PpuM I (RG/GACCY)Ava II, Sau96 IPpuM I (RG/GACCY)Ava II, Nla IV, Sau96 IPpuM I (RG/GTCCY)Ava II, BsmF I, Nla IV, Sau96 ISac I (GAGCT/C

25、)Ban II (GAGCT/C), BsiHKA I, Bsp1286 I (GAGCT/C)Alu I, Ban II, BsiHKA I, Bsp1286 I, Sac ISac II (CCGC/GG)BsiE I (CGGC/CG)Aci ISal I (G/TCGAC)Ava I (C/TCGAG), Xho ITaq ISbf I (CCTGCA/GG)BsiHKA I, Bsp1286 I (GTGCA/C)Bsg INsi I-Pst IPst ISfc I (C/TGCAG)ApaL IBsg ISgrA I (CR/CCGGYG)See BsrF ISpe I (A/CT

26、AGT)Avr II, Nhe I, Sty I (C/CTAGG), Xba IBfa ISph I (GCATG/C)Nla III, Nsp INla IIISty IAvr IIAvr II, Bfa I, BsaJ I, Sty I(C/CTAGG)Nhe I, Spe I, Xba IBfa I(C/CATGG)BspH INla IIINco IBsaJ I, Nco I, Nla III, Sty ITaq I (T/CGA)Acc I (GT/CGAC), Cla I, BstB ITaq IAci I, Acl I, BsaH I (GR/CGYC), HinP1 I,Hp

27、a II, Nar I-Tsp509 I (/AATT)Apo I (R/AATTY), EcoR I, Mfe ITsp509 IXba I (T/CTAGA)Avr II, Nhe I, Spe I, Sty I (C/CTAGG)Bfa IXho I/Tli I(C/TCGAG Ava I (C/TCGAG)Ava I, Taq I, Xho ISal ITaq IXma I (C/CCGGGAge I, BsaW I, BspE I, BsrF I, NgoM IV, SgrA IHpa II, Nci I, ScrF IAva I (C/CCGGG)Ava I, BsaJ I, Hpa II, Nci I, ScrF I, Sma I, Xma I