常用荧光团的参数与选择.docx

1、常用荧光团的参数与选择常用荧光团的参数与选择2015/4/17FluorophorePeakExcitation (nm)PeakEmission (nm)Intersection (nm)TypeCenterWavelength(nm)Optical Density ODBandwith(nm)MinimumTransmission(%)1,8-ANS (BSA)374.9477422.9Excitation37765085Emission480310452-dodecylresorufin (lipid)581.9595589.5Excitation58031045Emission5936

2、40905-(and-6)-carboxy-2,7-dichlorofluorescein (pH 9.0)504524.9513.8Excitation50531045Emission520635905-carboxytetramethylrhodamine 5-TAMRA (MeOH)542.9567554.4Excitation54362290Emission568310455-chloromethylfluorescein (pH 7.2)490.9517501.8Excitation49231045Emission515310455-FAM (pH 9.0)491.9518.1503

3、.5Excitation49231045Emission520635905-ROX (pH 7.0)578.2604589.7Excitation58031045Emission600380605-TAMRA (pH 7.0)553576563.4Excitation55038060Emission580310456-carboxyrhodamine 6G (pH 7.0)525547536Excitation52063590Emission546310456-JOE (pH 9.0)519.8548532.7Excitation52063590Emission550380607-amino-

4、4-methylcoumarin (pH 7.0)346442396.5Excitation34062675Emission442310407-aminoactinomycin D (DNA)548.1647.9601.8Excitation55038060Emission647310457-hydroxy-4-methylcoumarin (pH 9.0)360.1447.9403Excitation36531025Emission44766090Acridine orange (DNA)502.1526513Excitation50062490Emission53164090Alexa F

5、luor 350 goat anti-mouse IgG antibody (pH 8.0)343441392.9Excitation34062675Emission44231040Alexa Fluor 405 goat anti-mouse IgG antibody (pH 7.2)401.1422.1409Excitation40035055Emission42031040Alexa Fluor 430 goat anti-mouse IgG antibody (pH 7.2)431.2540492.9Excitation43031040Emission54031045Alexa Flu

6、or 488 dye-labeled oligonucleotide (H2O)493.1519505.8Excitation49231045Emission52063590Alexa Fluor 488 goat anti-mouse IgG antibody (pH 8.0)498.9520508.8Excitation50062490Emission52063590Alexa Fluor 488 hydrazide (H2O)492.8517.1504.9Excitation49231045Emission51531045Alexa Fluor 514 goat anti-mouse I

7、gG antibody (pH 8.0)518542.9529.8Excitation52063590Emission54362290Alexa Fluor 532 goat anti-mouse IgG antibody (pH 7.2)533.9553543Excitation53531045Emission55038060Alexa Fluor 546 dye-labeled oligonucleotide (H2O)554.1574563.8Excitation55038060Emission58031045Alexa Fluor 546 goat anti-mouse IgG ant

8、ibody (pH 7.2)561.3572566.9Excitation56264090Emission56831045Alexa Fluor 555 dye-labeled oligonucleotide (H2O)552.3567.1559.6Excitation55038060Emission56831045Alexa Fluor 555 goat anti-mouse IgG antibody (pH 7.2)553.2567.9560.2Excitation55038060Emission56831045Alexa Fluor 555 hydrazide (H2O)551.1564

9、557.1Excitation55038060Emission56264090Alexa Fluor 568 goat anti-mouse IgG antibody (pH 7.2)578.9603590.2Excitation58031045Emission60038060Alexa Fluor 568 hydrazide (H2O)572.9599.9585.8Excitation56831045Emission60038060Alexa Fluor 594 cadaverine (H2O)587.9614.1600.1Excitation58931045Emission61031045

10、Alexa Fluor 594 dye-labeled oligonucleotide (H2O)590.9621605.7Excitation58931045Emission62031045Alexa Fluor 594 goat anti-mouse IgG antibody (pH 7.2)590.2618.1604.2Excitation58931045Emission62031045Alexa Fluor 610 goat anti-mouse IgG antibody (pH 7.2)611.9630620.2Excitation61031045Emission62864090Al

11、exa Fluor 610-R-phycoerythrin streptavidin (pH 7.2)566.9627589.7Excitation56831045Emission62864090Alexa Fluor 633 goat anti-mouse IgG antibody (pH 7.2)631.1649.9640.2Excitation63241085Emission65041085Alexa Fluor 635 goat anti-mouse IgG antibody (pH 7.2)633.1647.9639.9Excitation63241085Emission647310

12、45Alexa Fluor 647 dye-labeled oligonucleotide (H2O)650.1667.9659.1Excitation65041085Emission67141085Alexa Fluor 647 goat anti-mouse IgG antibody (pH 7.2)649.9671660.6Excitation65041085Emission67141085Alexa Fluor 647 hydrazide (H2O)649.1670659.2Excitation65041085Emission67141085Alexa Fluor 647-R-phyc

13、oerythrin streptavidin (pH 7.2)568.2666648.9Excitation56831045Emission67141085Alexa Fluor 660 goat anti-mouse IgG antibody (pH 7.2)663.1690.9679.3Excitation65641085Emission69031050Alexa Fluor 680 goat anti-mouse IgG antibody (pH 7.2)680.9703.9692.8Excitation67631050Emission70038060Alexa Fluor 680-R-

14、phycoerythrin streptavidin (pH 7.2)569.6702687.1Excitation56831045Emission70038060Alexa Fluor 700 goat anti-mouse IgG antibody (pH 7.2)695.9719708.3Excitation69431050Emission73031050Alexa Fluor 750 goat anti-mouse IgG antibody (pH 7.2)752.4776.1763.6Excitation76631050Emission78031050Alexa Fluor 790

15、goat anti-mouse IgG antibody (pH 7.2)781.8804.9791.1Excitation78031050Emission80031050Alexa Fluorr 350346.9443.9394.2Excitation34062675Emission44231040Allophycocyanin (pH 7.5)651660.1655.6Excitation65041085Emission65641085Amplex UltraRed peroxidation product (pH 7.5)567.9581575.2Excitation56831045Em

16、ission58031045BOBO-1 (DNA)461.2483.9472.6Excitation45831045Emission48363290BOBO-3 (DNA)570.2605.1590.2Excitation56831045Emission61031045BODIPY 630-650-X (MeOH)625.2641633.7Excitation62464090Emission64031045BODIPY 650-665-X (MeOH)645.9664.1654.9Excitation64731045Emission67141085BODIPY FL (MeOH)502.15

17、11.1506.7Excitation50062490Emission51068490BODIPY FL goat anti-mouse IgG antibody (pH 7.2)505.3512504.1Excitation50531045Emission51068490BODIPY R6G (MeOH)528.2546.9539.2Excitation53164090Emission54631045BODIPY TMR-X (MeOH)544570.1559.3Excitation54362290Emission56831045BODIPY TMR-X goat anti-mouse Ig

18、G antibody (pH 7.2)544573561.4Excitation54362290Emission56831045BODIPY TR-X (MeOH)588621607.2Excitation58931045Emission62031045BODIPY TR-X phallacidin (pH 7.0)590621608.1Excitation58931045Emission62031045Calcein (pH 9.0)493.9514503.6Excitation49231045Emission51531045Calcium Crimson (Ca2+)589608.9598

19、.3Excitation58931045Emission61031045Calcium Green-1 (Ca2+)507529.1517Excitation50831045Emission53164090Calcium Orange (Ca2+)549.1574561.3Excitation55038060Emission56831045Carboxynaphthofluorescein (pH 10.0)598.9674637Excitation60038060Emission67631050Cascade Blue BSA (pH 7.0)401.1419407.8Excitation4

20、0035055Emission42031040Cascade Yellow goat anti-mouse IgG antibody (pH 8.0)399.1549.1472.4Excitation40035055Emission55038060CellMask Deep Red plasma membrane stain659675.9668.6Excitation65641085Emission67631050CellMask Orange plasma membrane stain556571.9563.5Excitation55038060Emission56831045CellTr

21、ace BODIPY TR methyl ester (lipid)597.9624.9613.5Excitation60038060Emission62464090CellTrace calcein violet (pH 7.2)400.1452.1427.7Excitation40035055Emission45038060CellTracker Red CMTPX586614597.1Excitation58931045Emission61031045CellTracker Violet BMQC+GSH404.1525.9470.2Excitation40541085Emission5

22、3164090CFP (pH 7.4)433.1475.1465.3Excitation43631040Emission47263090CMR (pH 7.4)547574.9560.5Excitation54631045Emission58031045Cy3 dye-labeled IgG antibody (pH 7.2)553.9565.8559.5Excitation55038060Emission56831045Cy5 dye-labeled IgG antibody (pH 7.2)648.9666.1657.9Excitation65041085Emission67141085C

23、yQUA GR (DNA)502.1522.9512.2Excitation50062490Emission52063590DAF-FM (NO)495519505.4Excitation49231045Emission52063590DAPI (DNA)359460.9401.1Excitation36531025Emission45831045Dapoxyl (2-aminoethyl)sulfonamide (MeOH)373.1578.9459.2Excitation37765085Emission58031045DDAO (pH 9.0)647.7657.1653.4Excitati

24、on64731045Emission65641085Di-8-ANEPPS (lipid)467631536.9Excitation46731045Emission63241085DiA (lipid)456.9585.9521.5Excitation45831045Emission58931045DiD (lipid)647.9669.9658.3Excitation64731045Emission67141085DiFMU (pH 3.0)319.9451.1370.4Excitation33431025Emission45038060DiFMU (pH 9.0)358450406.5Excitation36531025Emission45038060DiI (lipid)550.9568.9559.6Excitation55038060Emission56831045DiO (lipid)489.2506497.8Excitation488