Diethylnitrosamineinduced rat liver cancer model and pathological changes毕业论文翻译.docx

1、Diethylnitrosamineinduced rat liver cancer model and pathological changes毕业论文翻译 Diethylnitrosamine-induced rat liver cancer model and pathological changesPaper to write network: : Li Xiao Yan white salty Yong Liu Shen Han Yuzhen Liu Lide Abstract Objective To establish a simple occurrence of a simil

2、ar process of development of liver cancer animal models and human liver cancer. Methods weighing 150 to 200 g male Wistar rats of 150 were randomly divided into experimental and control groups, the experimental group was fed with containing diethylnitrosamine (DEN, 80 10-6), drinking water, changed

3、to free access to water for 12 weeks. control group of conventional free access to water. the results of pathological examination confirmed that DEN-induced liver cancer is hepatocellular carcinoma, induced cancer rate 70.6% (36/51, rat liver cancer carcinogenesis roughly three stages of liver cell

4、injury of the liver cell proliferation sclerosis and malignant transformation of conclusions fed low doses of DEN-induced rat liver cancer model to build a successful rate, is an ideal study of human liver cancer in animal models. Keywords: diethylnitrosamine, hepatocellular carcinoma, rat, animal m

5、odels Abstract Objective To establish an experimental hepatocellular carcinoma rat model which is convenient and similar to the human hepatocarcinogenesis. Methods one hundred and fifty male Wistar rats, weighing 150 to 200g, were randomly divided into two groups. The experimental groups drinking wa

6、ter contained 80ppm diethylnitrosamine for 12 continuous weeks. The control drank the routine table water. Results 70.6% (36/51) hepatocellular carcinoma were induced by DEN. The procession of hepatocarcinogenesis in this model included three stages hepatic toxic lesion, hepatic proliferation / cirr

7、hosis and hepatic carcinogenesis . Conclusion The successful rate of liver cancer model induced by low dose DEN was high, it is an ideal experimental model for the study of hepatocarcinogenesis. Keywords: Diethylnitrosamine, hepatocellular carcinoma, rat, animal model Hepatocellular carcinoma (of he

8、patocellular carcinoma, HCC is one of the common malignant tumors, known as “cancer” from the perspective of tumor immunology, molecular biology and molecular pathology to study the occurrence, the law of development, and molecular mechanism, often subject to the constraints of clinical specimens. i

9、n liver cancer basic research, we need to choose a suitable liver cancer animal model to simulate the process of human liver disease to compensate for the lack of clinical specimens. This article describes compared with long period of application of low doses of diethylnitrosamine-induced pathologic

10、al changes in rat hepatoma model and the process of carcinogenesis. 1 Materials and methods 1.1 Drug diethylnitrosamine, 0.95 g / ml 99.9% purity, Sigma, USA. Male Wistar rats of clean grade of 1.2 in experimental animals 150 Animal Certificate of Conformity: SCXK (Lu) 20030004, weighing 150 to 200

11、g, were purchased from the Experimental Animal Center of Shandong University. All rats were divided cage rearing, use of standardized light, freedom eating, regular replacement of the litter, and adapt to the environment for 7 days. all the rats were divided into a random number table, the experimen

12、tal group (135) and the control group (15. Feed containing by DEN (80 10-6 drinking water for 1.3 experimental method experimental group, 12 weeks into free drinking water. Control group, conventional free access to water daily observation of the animals mental state, diet and back gross changes in

13、general . experimental section 4,8,12,16,18,20,22,24,25 weeks were randomly selected from the model group 10 to 15, the abdominal cavity was opened, the general observation of the exposed liver, the record of the liver shape, color, texture. liver specimens, 4% paraformaldehyde buffer, fixed for 24

14、h, embedded in paraffin sections, HE staining, observed under the microscope. control group were taken at 4,12 and 24 weeks 5 to be treated in the same . 2 Results 2.1 General situation of carcinogenesis four weeks after the experimental group of Wistar rats food intake decreased, and the gradual em

15、ergence of the coat dull yellow, dull, slow, slow growth of body weight than the control group, some body weight of rats is significantly lower self-induced cancer, the first seven weeks, experimental rats began to death, total death of 24 Wistar rats, in particular paragraphs 17, 18 weeks and 21 to

16、 25 weeks more dead rats, the mortality rate was 17.78% (24/135. control the rats grew well, no significant pathological performance. 2.2 induced carcinogenesis in rat liver pathological changes 2.2.1 control group rats were no obvious abnormalities: liver naked eye view of the light microscope, liv

17、er tissue was normal, the liver cells arranged in cords around the central lobular vein radially arranged hepatocytes eosinophilic cytoplasm, nuclear basophilic , mostly for single-core, located in the cell center (see Figure 1. 2.2.2 experimental rats: experimental rats liver pathological changes c

18、an be broadly grouped into three: induced cancer early - liver cell injury period: 1 to 8 weeks (n = 30. Liver showed no abnormal table view the light microscope, swelling of the liver cells, showing diffuse liver cell edema, the part of the liver cells was eosinophilic chronotropic (see Figure 2. l

19、obule structure is still intact and visible focal lobular necrosis with inflammatory cell infiltration, while gradually fibrous tissue proliferation and regeneration. carcinogenesis interim - sclerosis period: 9 to 16 weeks (n = 30 liver surface gradually rough, there varying amounts, large and smal

20、l gray near-circular lesion diameter mostly below 1 mm, maximum up to about 5 mm, more dispersed (see Figure 3. first 16 weeks of cirrhosis, part of the lobe of cholestasis. light microscope, further aggravating the liver cell edema, visible vesicles of different degrees of steatosis. Share the free

21、 paper eosinophilic or clear cell foci (Figure 4), and gradually formed the nodules of liver cell proliferation, cellular atypia smaller nodules on the surrounding organizations oppression (see Figure 5. two cases showed a nodule of atypical hyperplasia, nodular liver cell dysplasia obvious cell nod

22、ules occasionally mitotic and nucleolar proliferation (see Figure 6. portal vein dilatation and congestion, there are varying degrees of oval cell proliferation and hyperplasia of connective tissue extended to the lobules, along with bile duct hyperplasia, increased number of visible the typical pse

23、udolobule form (see Figure 7. late carcinogenesis - cancer of 12 weeks one cases of rat peritoneal vivisection: 17 to 25 weeks (n = 51 gray nodules of the liver surface covered with several different sizes, and the largest 4.5 cm 3 cm 2 cm, the section can be seen bleeding and necrosis. appear withi

24、n the hemorrhagic ascites, dead rats in the process of carcinogenesis .1 cases of bloody ascites greater omentum in two cases of intra-abdominal diffuse nodular liver with adhesion. light microscope, liver cancer cells were arranged in beam cords and massive infiltration to the surrounding liver tis

25、sue, can be seen varying degrees of fatty degeneration, some areas of hemorrhage, necrosis (see Figure 8. cancer tissue edema, eosinophilic change, fatty degeneration, and foci of liver cell proliferation Results section and atypical hyperplasia nodular histological main types of hepatocellular carc

26、inoma, and one cases of mixed cell carcinoma 2.3 carcinogenesis process in various stages of lesion type and lesion type and incidence of the various stages of the incidence of DEN-induced rat liver cancer in Table 1. 3 Liver cancer animal model of choice is an important part of liver cancer experim

27、ental studies since the early 1900s, the deepening of spontaneous hepatocellular carcinoma in mice models of liver cancer animal model, there has been research, and gradually established a spontaneous animal liver cancer model induced liver cancer model, the transplanted animal liver cancer model, a

28、s well as transgenic animal liver cancer model. a variety of liver cancer animal model has its own characteristics and uses, the appropriate choice of liver cancer animal model according to the study purpose. Induced liver cancer model is a carcinogenic chemical, physical, or biological factors of t

29、he formation of liver cancer in animal models. Among them, the DEN-induced animal liver cancer is a broader application of an induced liver cancer model. By DEN for international Cancer Research Center institutions (International agency for research on cancer, carcinogens identified by IARC, the dua

30、l role of initiation and promotion of tumorigenesis. application of DEN-induced rat liver cancer model generally gavage once a week 0.25% by DEN aqueous solution 10 mg / kg body weight, the remaining 6 d fed drinking water to 0.025% by DEN 1, or transoral intubation were fed 0.2% by DEN solution, 1

31、ml of a day, a week continuously fed for 4 d, continuous fed 18 weeks after discontinuation 2, or 5 times a week, rats were given 0.2% by DEN (10 mg / kg, administered orally, modeling the way 3, but such method is more difficult, and long-term feeding on large The mouse body has a certain degree of

32、 damage, easily lead to the mouth and digestive tract infections. In this study, the feed containing by DEN (80 10-6 water-induced rat liver cancer model, has the following characteristics: operation more convenient, shorter cycle times, induced cancer rate (72.5% (2) carcinogenic than the loyal, he

33、patocellular carcinoma proportion accounted for 97.3% (37 cases of induced cancer, only one case of mixed-cell carcinoma, all the rest of HCC. cancerous process, the process of cirrhosis, the mid-carcinogenesis cirrhosis was 23.3%, 17.6% in carcinogenesis of hepatocellular carcinoma with cirrhosis, the pathologica