常用细胞培养基配方及缓冲液.docx

1、常用细胞培养基配方及缓冲液1640细胞培养基成分（mg/L）MD800MD801MD802MD803四水硝酸钙100100100100氯化钾400400400400无水硫酸镁48.8448.8448.8448.84氯化钠6000600060005850磷酸氢二钠800800800L-精氨酸200200200200L-天门冬酰胺50505050L-天门冬氨酸20202020L-胱氨酸盐酸盐65.1565.1565.1565.15L-谷氨酸20202020L-谷氨酰胺300300300300甘氨酸10101010L-组氨酸15151515L-羟脯氨酸20202020L-异亮氨酸50505050L-

2、亮氨酸50505050L-赖氨酸盐酸盐40404040L-蛋氨酸15151515L-苯丙氨酸15151515L-脯氨酸20202020L-丝氨酸30303030L-苏氨酸20202020L-色氨酸5555L-酪氨酸20202020L-缬氨酸20202020D-葡萄糖2000200020002000谷胱甘肽（还原型）1111HEPES5957.5酚红55维生素H0.20.20.20.2D-泛酸钙0.250.250.250.25氯化胆碱3333叶酸1111i-肌醇35353535烟酰胺1111对氨基苯甲酸1111盐酸吡哆醇1111核黄素0.20.20.20.2盐酸硫胺1111维生素B120.005

3、0.0050.0050.005pH（无碳酸氢钠）7.50.37.50.38.00.36.70.3pH（加碳酸氢钠）7.80.37.80.38.20.37.00.3渗透压（无碳酸氢钠）2375%2355%2355%2605%渗透压（加碳酸氢钠）2795%2805%2805%2925%以上数据仅为参考，以产品说明书为准。MEM细胞培养基成分（mg/L）MD600MD601MD602MD603MD604MD605MD606MD607MD608氯化钙200140200140200200200氯化钾400400400400400400400400400无水硫酸镁97.6797.6797.6797.679

4、7.6797.6797.6797.6797.67磷酸二氢钾6060氯化钠680080006800680080006800680068006800无水磷酸二氢钠121.74121.74121.74121.74121.74121.74磷酸氢二钠47.8847.88L-丙氨酸8.98.9L-精氨酸盐酸盐126126126126126126126126126L-天门冬酰胺13.213.2L-天门冬氨酸13.213.2L-胱氨酸盐酸盐31.2931.2931.2931.2931.29313131.2931.29L-谷氨酸14.714.7L-谷氨酰胺292292292292292292292甘氨酸7.57

5、.5L-组氨酸盐酸盐424242424242424242L-异亮氨酸525252525252525252L-亮氨酸5252525252525252L-赖氨酸盐酸盐72.572.572.572.572.572.572.572.5L-蛋氨酸1515151515151515L-苯丙氨酸323232323232323232L-脯氨酸11.511.5L-丝氨酸10.510.5L-苏氨酸484848484848484848L-色氨酸101010101010101010L-酪氨酸363636363636363636L-缬氨酸464646464646464646D-葡萄糖1000100010001000100

6、01000100010001000酚红1010101010661010丁二酸钠100100丁二酸7575D-泛酸钙111111111重酒石酸胆碱1.81.8氯化胆碱1111111叶酸111111111i-肌醇222222222烟酰胺111111111盐酸吡哆醛111111111核黄素0.10.10.10.10.10.10.10.10.1盐酸硫胺111111111pH（无碳酸氢钠）5.90.36.20.35.10.35.10.36.30.34.20.34.30.35.90.36.60.3pH（加碳酸氢钠）7.60.37.10.36.80.37.50.37.20.37.30.36.70.37.50

7、.37.80.3渗透压（无碳酸氢钠）250528252655250528752515267525052405渗透压（加碳酸氢钠）295528553005294530052895299529052815以上数据仅为参考，以产品说明书为准。DMEM/F12细胞培养基成分（mg/L）MD206MD207成分（mg/L）MD206MD207无水氯化钙116.6116.6L-色氨酸9.029.02五水硫酸铜0.00130.0013L-酪氨酸38.438.4九水硝酸铁0.050.05L-缬氨酸52.8552.85七水硫酸亚铁0.4170.417D-葡萄糖31513151氯化钾311.8311.8HEPES

8、3574.5氯化镁28.6428.64次黄嘌呤22无水硫酸镁48.8448.84亚油酸0.0420.042氯化钠6999.56999.5硫辛酸0.1050.105无水磷酸二氢钠54.3554.35酚红8.18.1磷酸氢二钠71.0271.021,4-丁二胺二盐酸盐0.0810.081七水硫酸锌0.4320.432丙酮酸钠5555L-精氨酸盐酸盐147.5147.5维生素H0.00350.0035L-胱氨酸盐酸盐31.2931.29D-泛酸钙2.242.24L-谷氨酰胺365365氯化胆碱8.988.98甘氨酸18.7518.75叶酸2.652.65L-组氨酸盐酸盐31.4831.48i-肌醇1

9、2.612.6L-异亮氨酸54.4754.47烟酰胺2.022.02L-亮氨酸59.0559.05盐酸吡哆醛22L-赖氨酸盐酸盐91.2591.25盐酸吡哆醇0.0310.031L-蛋氨酸17.2417.24核黄素0.2190.219L-苯丙氨酸35.4835.48盐酸硫胺2.172.17L-丝氨酸26.2526.25胸苷0.3650.365L-苏氨酸53.4553.45维生素B120.680.68L-丙氨酸4.454.45L-天门冬酰胺7.57.5pH（无碳酸氢钠）5.80.35.80.3L-天门冬氨酸6.656.65pH（加碳酸氢钠）6.80.36.90.3L-半胱氨酸盐酸盐17.5617.56渗透压（无碳酸氢钠）2795%2775%L-谷氨酸7.357.35渗透压（加碳酸氢钠）2995%3005%L-脯氨酸17.2517.25以上数据仅为参考，以产品说明书为准。IMDM细胞培养基成分（mg/L）MD400成分（mg/L）MD400氯化钙165L-苏氨酸95氯化钾330L-色氨酸16硝酸钾0.076L-酪氨酸71.5无水硫酸镁97.67L-缬氨酸94氯化钠4505D-葡萄糖4500无水磷酸二氢钠108.7酚红15五水亚硒酸钠0.0173HEPES5958L-