测序通用引物.docx

1、测序通用引物载体名称上游引物（5primer）下游引物（3primer）P3*FLAG-CMV CMV-30(825-854) CMV-24(1129-1152)pAc5.1/V5-His(_A,_B,_C)pAc5-5BGH revpAcG2TpGEX-5not availablepACT T7EEVT3/EBVrevpACT2 GAL4 ADforpGAL4-ADrvpACT2p17110p12584pACYC184(BamHI-site) PBRforBamPBRrevBampACYCDuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1pACYCDuet-1(

2、MCSII) DuetUp2T7-TerminatorpAdEasy-1not availablenot availablepAdTrack-CMVnot availablenot availablepAS2-1 GAL4 Bdfor/P24990pGAL4-BDrv/P31430pB42AD pB42ADF PB42ADRpBABE pBMN_5pBacPAK8 BAC1 BAC2pBADpBAD-FpBAD-RpBAD/HisABCpBAD-5pBBR1MCST3/M13revT7/M13forpBD-GAL4 pBD-GAL4-F PBD-GAL4-RpBI121 35S NOSpBIN

3、DGAL4-BdforT3,EBVrevpBK-CMV M13F/T7 M13R/T3pBluescriptIIKS(+/-)T3/M13revT7/M13for（优先用M13F）pBluescriptIISK(+/-)T3/M13revT7/M13forpBluescriptKS(+/-)T3/M13revT7/M13forpBluescriptSK(+/-)T3/M13revT7/M13forpBR322(BamHI-site) PBRforBamPBRrevBampBR322(EcoRI/HindIII-sites) pBRforEcopBRrevHindpBR325(BamHI-sit

4、e) PBRforBamPBRrevBampBR329(BamHI-site) PBRforBamPBRrevBampBridge(MCSI)GAL4-Bdfor3BDpBridge(MCSII)not availablenot availablepBS-T IIT3/M13revT7/M13forpBS（AMP）T7T3pBSR T3 T7terpBudCE4.1(MCSI)CMV-Profor,T7c-mycrev,EBVrevpBudCE4.1(MCSII)EF-1 FwdBGHpBudCE4.1/lacZ/CATCMV-Profor,T7c-mycrev,EBVrevpBV220 pB

5、V220F PBV220RpCAL-cT7/Tac-ProforT7-TerminatorpCAL-nT7/Tac-ProforT7-TerminatorpCAMBIA1301(1300)pCAMBIA1301-5(ECORI)/M13RpCAMBIA1301-3(HindIII)pCAMBIA1304pCAMBIA1301-5(ECORI)pCAMBIA1301-3(HindIII)pCAMBIA2300 M13R(-48) M13F(-47)/M13F(-49)pCANTB5E S1 S6pCAT3-enhancer RVP3 此载体无反向引物pCDFDuet-1(MCSI)ACYCDue

6、tUP1 Primer DuetDOWN1pCDFDuet-1(MCSII) DuetUP2 T7TERpcDNA1.1CMV-Profor,T7 pCDM8-3pcDNA2.1M13for/T7 M13rev/Tac-ProforpcDNA3T7/CMV-ProforBGH rev/SP6pcDNA3.1(+/-)pcDNA3.1F/T7/CMV-ProforBGH revpcDNA3.1/His(_A,_B,_C)T7/CMV-ProforBGH revpcDNA3.1/myc-His(_A,_B,_C)T7/CMV-ProforBGH rev/c-mycrevpcDNA3.1/V5-Hi

7、s(_A,_B,_C)T7/CMV-ProforBGH rev/V5revpcDNA3.1/Hygro(+,-)T7/CMV-ProforBGH rev/V5revpcDNA3.1/Zeo(+.-) T7BGHrevpcDNA3.3TOPOTACMV-ProforTKPAreversepcDNA4/HisMax(_A,_B,_C) XpressforBGH revpcDNA4/HisMax-TOPO XpressforBGH revpcDNA4/HisMax-TOPO/lacZ XpressforBGH revpcDNA4/myc-His(_A,_B,_C) T7,CMV-ProforBGH

8、rev,c-mycrevpcDNA4/TOTREfor/CMV-ProforBGH revpcDNA4/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5revpcDNA4-TET/ON/AMP+CMV-ProforpcDNA5/FRTT7/CMV-ProforBGH revpcDNA6/myc-His(_A,_B,_C)CMV-Profor,T7BGH rev,c-mycrevpcDNA6/V5-His(_A,_B,_C)CMV-Profor,T7BGH rev,V5revpCEP4 pCEP-F/CMV-ProforEBVrevpCIT7（17BASE）EBVr

9、evpCI-neoT7（17BASE）EBVrev,T3pCMS-EGFP T7 T3 pCMS-EGFPT7EBVrev,T3pCMV-3Tag-4A T3 /PFLAG-CMV-F T7pCMV5 pCMV5F PCMV5RpCMV6CMV-ProforpCMV6-3、HGHrevpCMV6-XL4CMV-Profor/T7pCMV6-3、HGHrevpCMV-HA PCMV-F,CMV-Profor,TREfor PCMV-R，EBVrevpCMV-MYC(AMP+) PCMV-F PCMV-R,EBVrevpCMVmyc/nucCMV-ProforBGH rev,c-mycrevpcm

10、v-scriptEXVectorT3T7pCMV-Sport6T7/M13forSP6/M13revpCOLADuet-1(MCSI)ACYCDuetUP1 Primer DuetDOWN1pCOLADuet-1(MCSII) DuetUP2 T7TERpCR2.1T7,M13forM13revpCR2.1-TOPOM13for,T7M13revpCR3.1T7BGH revpCR3.1-UniT7BGH revpCR4T3/M13revT7/M13forpCR4-BluntT3/M13revT7/M13forpCR4-TOPOM13rev,T3M13for,T7pCR-BluntM13rev

11、T7,M13forpCR-BluntIIM13rev/SP6T7/M13forpCR-BluntII-TOPOM13rev/SP6M13for/T7pCRIIM13rev/SP6T7/M13forpCRII-TOPOM13rev/SP6M13for/T7pCR-ScriptSK(+)T7/M13forT3/M13revpCR-XL-TOPOM13revT7,M13forpCS2+(MCSI)SP6 EBVrev,T7?pCS2+(MCSII)T3pSG5-3pDB_GAL4camT7PpDB_leuPDBLeu-5PDBLeu-3pDEST22DEST22FDEST22RpDEST15pGEX

12、-5T7-TerminatorpDEST20pGEX-5pFastBac-3pDEST26TREfor/CMV-ProforT7/M13forpDisplayT7/CMV-Proforc-mycrevpDNR-LIB(MCS_A)M13for,T7pSG5-3,M13RpDonar M13F M13RpDONR201 PDONR-F PDONR-RpDONR207 PDONR-F PDONR-RpDONR223 M13F M13R pDriveT7/M13revSP6/M13forpDsRED1-C1 (DsRed1-N-f) DsRed1-C-rpDsRed1-N1CMV-ProforRFP

13、-Nrev,CMV-R?pDsRed2-N1 (DsRed1-N-f) DsRed1-C-rpDsRed2-C1 (DsRed1-N-f) DsRed1-C-rpDsRED2-C1(KAN+) pDsRED-ex-C1-F pEGFP-N-3pDsRed-Express dsRed1_N_primer/CMV-F dsRed1_C_primer/CMV-RpDSRED-monomer-N1(KAN+) dsRed1_N_primerpDSRED-N1(KAN+) pEGFP-N-5 pDSRED-N-R pEASY-Blunt M13FT7/ M13RpEASY-BluntSimple M13FT7/ M13R（距离插入位点合适，优先安排）pEASY-T1 Simple M13FM13R（距离插入位点合适，优先安排）pEASY-T1 M13F/T7 M13RpEASY-T3 M13F/T7 M13R/SP6pECFP-N(C) PECFP-N