硕士生物仪器分析之气相色谱.ppt

1、气相色谱Gas Chromatography(GC)简介简介特点特点基本基本理论理论仪器流程及主要部件仪器流程及主要部件应用应用发展趋势发展趋势ChromatographyIsatechniqueusedtoseparateandidentifythecomponentsofamixture.Worksbyallowingthemoleculespresentinthemixturetodistributethemselvesbetweenastationaryandamobilemedium.Moleculesthatspendmostoftheirtimeinthemobilephasea

2、recarriedalongfaster.IntroductionGasChromatographyHerethemobilephaseisanunreactivegas(egNitrogen)flowingthroughatube.Andthestationaryphaseisaninvolatileliquid heldonparticlesofasolidsupport.IntroductionGasChromatography(GC)isnowamature analytical tool particularlyusefulintheanalysisofvolatileandther

3、mallystableanalytes.Characteristic 气相色谱可用来分析能能瞬瞬间间气气化化且且不不分分解解的、的、沸点0.1%)GC analysis of fragrances格拉斯格拉斯(Grasse)世界香水之都世界香水之都 提问时间Future Trends in the Separation Sciences1.MiniaturizationComparisonamongGCcolumns(inscale)ComparisonamongHPLCcolumns(inscale)packed columnscapillary columns2.Neither tempe

4、rature,nor pressure:electrical field GCthecolumntemperatureHPLCapressuregradientsolventselectrodrivenelectrophoretictechniques(hybridtechniquesthatuseaspectsofchromatographyaswellasaspectsofelectrophoresis)Future Trends in the Separation Sciences3.Hyphenated systemsLC-LC(suchas:aRP-18column+achiralc

5、olumn)SFE-CESFE-SPE-HRGCLC-GCSPME-GC-MSSFE-SFCFuture Trends in the Separation Sciences4.The role of the solvent in separation sciencesFuture Trends in the Separation Scienceshttp:/www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-505320030002000055.High speed separation techniques6.Automation of

6、 the separation techniques7.Unified chromatographyFuture Trends in the Separation SciencesGeneralseparationapproachemployedinatypicalcomplexanalysis1.ExtractionofsolidsamplesExtraction TechniquesSoxhletextractionMicrowaveextractionSonicationandsimilartechniquesPressurizedsolventtechniqueSupercritica

7、lfluidextraction(SFE)Subcriticalwaterextraction(SWE)Acceleratedsolventextraction(ASE)2.ExtractionofliquidsamplesLiquid-liquidextraction(LLE)Solid-phaseextraction(SPE)Solidphasemicroextraction(SPME)Stirbarsorptiveextraction(SBSE)Pressurizedsolventextraction(PSE)SFESWEASEExtraction Techniques3.ExtractionofgaseoussamplesHeadspacetechniquethestaticheadspace(SH)approachesthedynamicheadspace(DH)approaches Headspace-Solidphasemicroextraction(HS-SPME)Extraction Techniques