多肽设计原理和方法_精品文档.ppt

1、多肽设计原则和多肽设计原则和方法方法多肽抗原的特点多肽抗原的特点l含有的epitope数目少；l抗原性比融合蛋白弱；l产生的抗原特异性通常比融合蛋白强；l是一种半抗原，需要连接载体蛋白；多肽设计的原则多肽设计的原则lHydrophilicity,tobesuretheantigenisonthesurfaceoftheprotein.lAntigenicity,aminoacidprovidesgoodbonds.lN-orC-terminalpeptide,fortheyareexposedinmostproteins.lContainsMHCIIpreferredmotifs.Suchas

2、D,Y,P,andFwhoappearsinMHCmoleculesmostfrequently.lNohomologywithotherproteinsbyblast.lPreferthecytoplasmicdomainforatransmembraneprotein,fortheyaremoreimmunogeniclAvoidglycosylationandphosphorylationsitesifpossiblelAvoidcystininthemiddlepartofthepeptide20种氨基酸的抗原性比较种氨基酸的抗原性比较L0.75 K-0.05H0.65 P-0.05D

3、0.31 R-0.07T0.21 W-0.11A0.12 C-0.12E0.06 N-0.14Y0.01 G-0.18V-0.01 M-0.38S-0.01 F-0.77Q-0.03 I-2.92什么时候选用多肽做抗原什么时候选用多肽做抗原l我们没有基因的抗原；l有基因，但是蛋白质和其他蛋白质有较强的同源性，或者蛋白质属于同源性较强的蛋白家族；l有基因，需要区分该基因编码的蛋白质加工后的不同亚型；l抗原是修饰蛋白质，包括磷酸化，乙酰基化蛋白质等案例案例1 我们没有的蛋白质我们没有的蛋白质lNM_004445，EPHB6lMATEGAAQLGNRVAGMVCSLWVLLLVSSVLALEEVLL

4、DTTGETSEIGWLTYPPGGWDEVSVLDDQRRLTRTFEACHVAGAPPGTGQDNWLQTHFVERRGAQRAHIRLHFSVRACSSLGVSGGTCRETFTLYYRQAEEPDSPDSVSSWHLKRWTKVDTIAADESFPSSSSSSSSSSSAAWAVGPHGAGQRAGLQLNVKERSFGPLTQRGFYVAFQDTGACLALVAVRLFSYTCPAVLRSFASFPETQASGAGGASLVAAVGTCVAHAEPEEDGVGGQAGGSPPRLHCNGEGKWMVAVGGCRCQPGYQPARGDKACQACPRGLYKSSAGNAPCSPCPAR

5、SHAPNPAAPVCPCLEGFYRASSDPPEAPCTGPPSAPQELWFEVQGSALMLHWRLPRELGGRGDLLFNVVCKECEGRQEPASGGGGTCHRCRDEVHFDPRQRGLTESRVLVGGLRAHVPYILEVQAVNGVSELSPDPPQAAAINVSTSHEVPSAVPVVHQVSRASNSITVSWPQPDQTNGNILDYQLRYYDQAEDESHSFTLTSETNTATVTQLSPGHIYGFQVRARTAAGHGPYGGKVYFQTLPQGELSSQLPERLSLVIGSILGALAFLLLAAITVLAVVFQRKRRGTGYTEQLQQYSS

6、PGLGVKYYIDPSTYEDPCQAIRELAREVDPAYIKIEEVIGTGSFGEVRQGRLQPRGRREQTVAIQALWAGGAESLQMTFLGRAAVLGQFQHPNILRLEGVVTKSRPLMVLTEFMELGPLDSFLRQREGQFSSLQLVAMQRGVAAAMQYLSSFAFVHRSLSAHSVLVNSHLVCKVARLGHSPQGPSCLLRWAAPEVIAHGKHTTSSDVWSFGILMWEVMSYGERPYWDMSEQEVLNAIEQEFRLPPPPGCPPGLHLLMLDTWQKDRARRPHFDQLVAAFDKMIRKPDTLQAGGDPGERPSQ

7、ALLTPVALDFPCLDSPQAWLSAIGLECYQDNFSKFGLCTFSDVAQLSLEDLPALGITLAGHQKKLLHHIQLLQQHLRQQGSVEVl共1021aa，N-15aa为信号肽案例案例1 我们没有的蛋白质（我们没有的蛋白质（2）lWDEVSVLDDQRRLTRTFEAC案例案例1 我们没有的蛋白质（我们没有的蛋白质（3）lGENEID:2051EPHB6|EPHreceptorB6HomosapienslQuery1WDEVSVLDDQRRLTRTFEAC20lWDEVSVLDDQRRLTRTFEAClSbjct41WDEVSVLDDQRRLTRTFEAC60lG

8、ENEID:814CAMK4|calcium/calmodulin-dependentproteinkinaseIVlQuery1lWDEVS-VLDDQRRLTRTFEA19lWDEVSVLD+RLTTF+ASbjct69WDEVSLNAKDLVRKLIVLDPKKRLT-TFQA97案例案例2 家族性蛋白的多肽设计家族性蛋白的多肽设计（transgelin）lThetransgelinfamilyisagroupofproteinsthatbelongto22kdactin-relatedcorpninsuperfamily.lOfallthreeisoforms,transgelin1i

9、sthebestcharacterized.Transgelin1,alsoknownasSM22alpha,isaspecificmarkerfordifferentiatedsmoothmusclecells.lTransgelin2,alsoknownasSM22beta,isexpressedbybothsmoothmuscleandnon-smoothmusclecellsinatemporallyandspatiallyregulatedpattern.lTrangenlin3,alsoknownasNP25,isonlyfoundinhighlydifferentiatedneu

10、ronalcells.案例案例2家族性蛋白的多肽设计家族性蛋白的多肽设计（transgelin）2案例案例2 家族性蛋白的多肽设计家族性蛋白的多肽设计（transgelin）3lTransgelin1:KYDEELEERLVEWIIVQClTransgelin2:QPGRENFQNWLKDGTVLC案例案例3 家族性蛋白的多肽设计家族性蛋白的多肽设计 RAB3A,3B,3ClSequence2:NM_002866,RAB3A,memberRASoncogenefamilylSequence3:NM_002867,RAB3B,memberRASoncogenefamilylSequence4:N

11、M_138453,RAB3C,memberRASoncogenefamily案例案例4 家族性蛋白的多肽设计家族性蛋白的多肽设计actin对应的处理方法？对应的处理方法？案例案例5 同一蛋白质不同亚型的多肽设计同一蛋白质不同亚型的多肽设计 NFKB1lNFKB1,lNUCLEAR FACTOR KAPPA-B,SUBUNIT 1;NFKB1lAlternative titles;symbols lTRANSCRIPTION FACTOR NFKB1NUCLEAR FACTOR OF KAPPA LIGHT CHAIN GENE ENHANCER IN B CELLS 1NFKB p105,IN

12、CLUDEDNFKB p50,INCLUDED案例案例5 同一蛋白质不同亚型的多肽设计同一蛋白质不同亚型的多肽设计 NFKB1lThisgeneencodesa105kDproteinwhichcanundergocotranslationalprocessingbythe26Sproteasometoproducea50kDprotein.The105kDproteinisaRelprotein-specifictranscriptioninhibitorandthe50kDproteinisaDNAbindingsubunitoftheNF-kappa-B(NFKB)proteincomp

13、lex.l1433aminoacidsoffulllength968p105proteinformp50.案例案例5 同一蛋白质不同亚型的多肽设同一蛋白质不同亚型的多肽设计计 NFKB1lMAEDDPYLGRPEQMFHLDPSLTHTIFNPEVFQPQMALPTADGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLGDREKELIRQAALQQTK

14、EMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDINITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGTGSTGPGYSFPHYGFPTYGGITFHPGTTKSNAGMKHGTMDTESKKDPEGCDKSDDKNTVNLFGKVIETTEQDQEPSEATVGNGEVTLTYATGTKEESAGVQDNLFLEKA

15、MQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHSQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRAGADLSLLDRLGNSVLHLAAKEGHDKVLSILLKHKKAALLLDHPNGDGLNAIHLAMMSNSLPCLLLLVAAGADVNAQEQKSGRTALHLAVEHDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWENAGEDEGVVPGTTPLDMATSWQVFDILNGKPYEPEFTSDDLLAQ

16、GDMKQLAEDVKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI案例案例5 同一蛋白质不同亚型的多肽设同一蛋白质不同亚型的多肽设计计 NFKB1案例案例6 同一蛋白质不同亚型的多肽设计同一蛋白质不同亚型的多肽设计 CXCR3lTheCXCR3proteinbelongstoGprotein-coupledreceptorfamily.Severalalternativelysplicedtranscriptvariantsencodingdistinctisoformshavebeenreported.TheCXCR3Bproteincontains416aminoacidsandhasalongerNterminusthatdiffersfromtheoriginal368-aminoacidCXCR3protein,CXCR