1、3.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06.26.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.553.532.611.831.270.850.55
2、Na2HPO4分子量 = 14.98,0.2 mol/L溶液为28.40克/升。Na2HPO42H2O分子量 = 178.05,0.2 mol/L溶液含35.01克/升。C4H2O7H2O分子量 = 210.14,0.1 mol/L溶液为21.01克/升。4柠檬酸氢氧化钠-盐酸缓冲液钠离子浓度(mol/L)柠檬酸(克)C6H8O7H2O氢氧化钠(克)NaOH 97%盐酸(毫升)HCl(浓)最终体积(升)3.13.34.35.36.50.200.350.450.382102452852668483144186156160116106456810512610 使用时可以每升中加入1克克酚,若最后p
3、H值有变化,再用少量50%氢氧化钠溶液或浓盐酸调节,冰箱保存。5柠檬酸柠檬酸钠缓冲液(0.1 mol/L)0.1 mol/L柠檬酸钠18.617.216.014.914.013.112.310.39.21.45.16.97.78.69.710.87.35.54.711.812.713.614.515.316.218.0柠檬酸C6H8O7H2O:分子量210.14,0.1 mol/L溶液为21.01克/升。柠檬酸钠Na3 C6H5O72H2O:分子量294.12,0.1 mol/L溶液为29.41克/毫升。6乙酸乙酸钠缓冲液(0.2 mol/L)pH(18)0.2 NaAc0.3 HAcNaAc
4、HAc0.751.201.802.653.704.909.258.808.207.356.305.105.907.007.908.609.109.404.103.002.101.400.900.60Na2Ac3H2O分子量 = 136.09,0.2 mol/L溶液为27.22克/升。7磷酸盐缓冲液(1)磷酸氢二钠磷酸二氢钠缓冲液(0.2) Na2HPO4 NaH2PO4 mol/L Na2HPO45.96.16.36.710.015.018.522.526.531.537.543.549.555.092.090.087.785.081.577.573.568.562.5 56.551.045.
5、07.17.57.961.067.072.077.081.084.087.089.591.593.094.739.033.028.023.019.013.010.58.52H2O分子量 = 178.05,0.2 mol/L溶液为85.61克/升。12H2O分子量 = 358.22,0.2 mol/L溶液为71.64克/升。2H2O分子量 = 156.03,0.2 mol/L溶液为31.21克/升。(2)磷酸氢二钠磷酸二氢钾缓冲液(1/15 mol/L)mol/L/15Na2HPO4mol/L/15KH2PO4mol/L/15Na2HPO44.925.295.916.246.476.646.81
6、6.980.100.501.002.004.005.006.009.909.509.008.007.177.387.738.048.348.678.189.7510.000.252H2O分子量 = 178.05,1/15M溶液为11.876克/升。KH2PO4分子量 = 136.09,1/15M溶液为9.078克/升。8磷酸二氢钾氢氧化钠缓冲液(0.05M)X毫升0.2M K2PO4 + Y毫升0.2N NaOH加水稀释至29毫升 pH(20)X (毫升)Y(毫升)0.3720.5700.8601.2601.7802.3652.9633.5003.9504.2804.5204.6809巴比妥钠
7、-盐酸缓冲液(18)0.04M巴比妥钠溶液0.2V盐酸0.04M巴比妥钠溶液(毫升)0.2N盐酸(毫升)10018.417.816.713.411.479.397.218.48.89.09.49.65.213.822.521.651.130.70巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10Tris盐酸缓冲液(0.05M,25)50毫升0.1M三羟甲基氨基甲烷(Tris)溶液与X毫升0.1N盐酸混匀后,加水稀释至100毫升。X(毫升)7.207.307.407.507.607.707.8045.744.743.442.040.338.536.634.532.029.28.1
8、08.308.508.708.9026.222.919.914.712.4三羟甲基氨基甲烷(Tris)HOCH2 CH2OH CHOCH2 NH2分子量=121.14;0. 1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳,使用时注意将瓶盖严。11硼酸硼砂缓冲液(0.2M硼酸根)0.05M硼砂(毫升)0.2M硼砂(毫升)0.2M硼酸(毫升)1.01.58.73.54.5硼砂Na2B4O7H2O,分子量=381.43;0.05M溶液(=0.2M硼酸根)含19.07克/升。硼酸H2BO3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水,必须在带塞的瓶中保存。1
9、1硼酸-硼砂缓冲液(0.2M硼酸根)10H2O,分子量=381.43;硼酸H2BO3,分子量=61.84, 0.2M溶液为12.37克/升。硼砂 易失去结晶水,必须在带塞的瓶中保存。12甘氨酸氢氧化钠缓冲液(0.05M)X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升12.09.810.410.622.427.238.645.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升。13硼砂-氢氧化钠缓冲液(0.05M硼酸根)X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀释至200毫升9.311.010.134.043.046.00.05M溶液为19.07克/升。14碳酸
10、钠-碳酸氢钠缓冲液(0.1M)Ca2+、Mg2+存在时不得使用0.1MNa2CO3(毫升)0.1MN2HCO3(毫升)20379.169.519.7810.2810.5310.838.779.129.7210.0810.5712346789Na2CO210H2O分子量=286.2;0.1M溶液为28.62克/升。N2HCO3分子量=84.0;0.1M溶液为8.40克/升。15“PBS”缓冲液PHNaClNaH2PO410000.10.20.3100ml8.5g2.2g0.4g 附录四 常用缓冲溶液的配制 (一)磷酸氢二钠-柠檬酸缓冲液0.2 mol/LNa2HPO4 (mL)0.1 mol/L
11、柠檬酸 (mL)19.60Na2HPO4分子量141.98;0.2 mol/L溶液为28.40 g/L。2H2O分子量178.05;0.2 mol/L溶液为35.61 g/L。12H2O分子量358.22;0.2 mol/L溶液为71.64 g/L。H2O分子量210.14;0.1 mol/L溶液为21.01 g/L。(二)柠檬酸-柠檬酸钠缓冲液(0.1 mol/L)柠檬酸钠 (mL)柠檬酸:H2O分子量210.14 ;柠檬酸钠:Na3C6H5O72H2O分子量294.12 ;0.1 mol/L溶液为29.41 g/L。(三)醋酸-醋酸钠缓冲液(0.2 mol/L)(18)NaAc (mL)H
12、Ac (mL)NaAc6.40NaAc3H2O分子量136.09;0.2 mol/L溶液为27.22 g/L。冰乙酸11.8 mL稀释至1 L(需标定)。(四)磷酸二氢钾-氢氧化钠缓冲液(0.05 mol/L)X 毫升 0.2 mol/L KH2PO4+Y毫升0.2 mol/L NaOH 加水稀释至20毫升。pH (20)XY(五)磷酸氢二钠-磷酸二氢钠缓冲液(0.2 mol/L)Na2HPO4NaH2PO4 (mL)56.549.0NaH2PO4H2O分子量138.01;0.2 mol/L溶液为27.6 g/L。2H2O分子量156.03;0.2 mol/L溶液为31.21 g/L。(六)巴比妥纳-盐酸缓冲液0.04 mol/L巴比妥钠HCl (mL)11
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