限制性酶的酶切位点综述Word下载.docx

1、GCA AosII GR! ApaI GGGCC! ApaLI G!TGCAC ApyI CC!WGG AquI C!YCGRG AseI AT!TAAT AspI GAANN!NNTTC AspI G!GTACC AspI GACN!NNGTC AsuI G!GNCC AsuII TT!CGAA AvaI C! AvaII G! AvaIII ATGCA!T AvrI !CYCGRG AvrII C!CTAGG AxyI CC! BalI TGG!CCA BamHI G!GATCC BanI G!GYRCC BanII GRGCY! BanIII AT!CGAT BbeI GGCGC! Bb

2、iII/AcyI GR! BbvI GCAGCNNNNNNNN! BbvI !NNNNNNNNNNNNGCTGC BbvII GAAGACNN! BbvII !NNNNNNGTCTTC BcefI ACGGCNNNNNNNNNNNN! BcefI !NNNNNNNNNNNNNGCCGT BclI T!GATCA BcnI CC! BglI GCCNNNN!NGGC BglII A!GATCT BinI ! BinI GGATCNNNN! BsePI !GCGCGC BsmAI !GTCTC GAGAC BsmI GAATGCN! BsmI G!CATTC BspI GDGCH! BspHI T

3、!CATGA BspMI ACCTGCNNNN! BspMI !NNNNNNNNGCAGGT BspMII T! BsrI ACTGGN! BsrI C!CAGT BssHII G!CGCGC BstBI TT! BstEII G!GTNACC BstI G! BstNI CC! BstPI G! BstUI CG! BstXI CCANNNNN!NTGG BstYI R!GATCY BsuI CC! CcrI C!TCGAG CfoI GCG! CfrI R!CCGGY CfrI G! CfrI Y!GGCCR ClaI AT! CviJI RG!CY CvnI CC! DdeI C!TNA

4、G DpnI GA!TC DraI TTT! DraII RG!GNCCY DraIII CACNNN!GTG DsaI C!CRYGG EaeI Y! EagI C!GGCCG EarI !CTCTTC GAAGAG EclXI C! EcoI TAC!GTA EcoI GGTCTCN! EcoI !NNNNNGAGACC EcoI G! EcoIII AGC!GCT EcoI C!CTGAAG CTTCAG EcoI CC! EcoNI CCTNN!NNNAGG EcoOI RG! EcoRI G!AATTC EcoRII !CCWGG EcoRV GAT!ATC EcoTI C!CWWG

5、G EcoTI ATGCA! EcoTI GRGCY! EheI GGC!GCC EspI GC!TNAGC FinI !GTCCC GGGAC FnuHI GC!NGC FokI GGATGNNNNNNNNN! FokI !NNNNNNNNNNNNNCATCC FspI TGC! GdiII !NNNNNYGGCCG GdiII CGGCCRN! GsuI !CTCCAG CTGGAG HaeI WGG!CCW HaeII RGCGC!Y HaeIII GG!CC HapII C!CGG HgaI GACGCNNNNN! HgaI !NNNNNNNNNNGCGTC HgiAI GWGCW!

6、HgiEII !ACCNNNNNNGGT HhaI GCG! HinI GR! HinPI G!CGC HincII GTY!RAC HindIII A!AGCTT HinfI G!ANTC HpaI GTT!AAC HpaII C! HphI GGTGANNNNNNNN! HphI !NNNNNNNTCACC KpnI GGTAC! KspI CTCTTCN! KspI !NNNNGAAGAG MaeI C!TAG MaeII A!CGT MaeIII !GTNAC MboI !GATC MboII GAAGANNNNNNNN! MboII !NNNNNNNTCTTC MfeI !CAATT

7、G MflI R! MluI A!CGCGT MmeI !TCCRAC GTYGGA MnlI CCTCNNNNNNN! MnlI !NNNNNNNGAGG MroI T! MseI T!TAA MspI C! MstI TGC! MstII CC! MvaI CC! NaeI GCC!GGC NarI GG!CGCC NciI CC! NcoI C!CATGG NdeI CA!TATG NdeII ! NheI G!CTAGC NlaIII CATG! NlaIV GGN!NCC NotI GC!GGCCGC NruI TCG!CGA NsiI ATGCA! Nsp（）I RCATG! Ns

8、p（）V TT! NspBII CMG!CKG NspII GDGCH! NspIII C! NspIV G! NunII GG! PaeR C! PalI GG! PflMI CCANNNN! PleI GAGTCNNNN! PleI !NNNNNGACTC PmaCI CAC! PpuMI RG!GWCCY PstI CTGCA!G PvuI CGAT! PvuII CAG! RsaI GT!AC RsrI G! RsrII CG!GWCCG SacI GAGCT! SacII CCGC!GG SalI G!TCGAC SauAI ! SauI G! SauI CC! ScaI AGT!A

9、CT ScrFI CC!NGG SduI GDGCH! SecI C!CNNGG SexI !CTCGAG SfaNI GCATCNNNNN! SfaNI !NNNNNNNNNGATGC SfiI GGCCNNNN!NGGCC SinI G! SmaI CCC!GGG SnaBI TAC! SnaI !GTATAC SpeI A!CTAGT SphI GCATG! SplI C!GTACG SspI AAT!ATT SstI GAGCT! SstII CCGC! SstIII !ACGT StuI AGG! StyI C! StySJI !GAGNNNNNNGTRC GYACNNNNNNCTC TaqI T! TaqII GACCGANNNNNNNNNNN! TaqII !NNNNNNNNNTCGGTC TaqII CACCCANNNNNNNNNNN!NNNNNNNNNTGGGTG ThaI CG! TspI !GTSAC TspEI !AATT TthI GACN! TthII CAARCANNNNNNNNNNN! TthII !NNNNNNNNNTGYTTG TthHBI T! VspI AT! XbaI T!CTAGA XcyI C!CCGGG XhoI C! XhoII R! XmaI C! XmaIII C! XmnI GAANN! XorII CGAT!