常见缓冲溶液的配制Word文档格式.docx

1、4.24.44.64.80.401.242.183.174.114.945.706.447.107.718.288.829.359.8610.3010.6018.7617.8216.8315.8915.0614.3013.5612.9012.2911.7211.1810.6510.149.705.25.45.65.86.06.26.66.87.07.27.47.67.88.010.7211.1511.6012.0912.6313.2213.8514.5515.4516.4717.3918.1718.7319.1519.459.288.858.407.917.376.786.155.454.55

2、3.532.611.831.270.850.55Na2HPO4分子量 = 14.98，0.2 mol/L溶液为28.40克/升。Na2HPO4-2H2O分子量 = 178.05，0.2 mol/L溶液含35.01克/升。C4H2O7H2O分子量 = 210.14，0.1 mol/L溶液为21.01克/升。4柠檬酸氢氧化钠-盐酸缓冲液钠离子浓度（mol/L）柠檬酸（克）C6H8O7H2O氢氧化钠（克）NaOH 97%盐酸（毫升）HCl（浓）最终体积（升）3.13.34.35.36.50.200.350.450.3821024528526684831441861561601161064568105

3、126101 使用时可以每升中加入1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。2 5柠檬酸柠檬酸钠缓冲液（0.1 mol/L）1.1 0.1 mol/L柠檬酸钠18.617.216.014.914.013.112.310.39.21.45.16.97.78.69.710.87.35.54.711.812.713.614.515.316.218.0柠檬酸C6H8O7H2O：分子量210.14，0.1 mol/L溶液为21.01克/升。柠檬酸钠Na3 C6H5O72H2O：分子量294.12，0.1 mol/L溶液为29.41克/毫升。6乙酸乙酸钠缓冲液（0.2

4、 mol/L）pH（18）1.2 0.2 NaAc1.3 0.3 HAcNaAcHAc0.751.201.802.653.704.909.258.808.207.356.305.105.907.007.908.609.109.404.103.002.101.400.900.60Na2Ac3H2O分子量 = 136.09，0.2 mol/L溶液为27.22克/升。7磷酸盐缓冲液（1）磷酸氢二钠磷酸二氢钠缓冲液（0.2） Na2HPO4 NaH2PO4 mol/L Na2HPO45.96.16.36.710.015.018.522.526.531.537.543.549.555.092.090.0

5、87.785.081.577.573.568.562.5 56.551.045.07.17.57.961.067.072.077.081.084.087.089.591.593.094.739.033.028.023.019.013.010.58.5Na2HPO42H2O分子量 = 178.05，0.2 mol/L溶液为85.61克/升。2H2O分子量 = 358.22，0.2 mol/L溶液为71.64克/升。2H2O分子量 = 156.03，0.2 mol/L溶液为31.21克/升。（2）磷酸氢二钠磷酸二氢钾缓冲液（1/15 mol/L）mol/L/15Na2HPO4mol/L/15KH2

6、PO4mol/L/15Na2HPO44.925.295.916.246.476.646.816.980.100.501.002.004.005.006.009.909.509.008.007.177.387.738.048.348.678.189.7510.000.252H2O分子量 = 178.05，1/15M溶液为11.876克/升。KH2PO4分子量 = 136.09，1/15M溶液为9.078克/升。8磷酸二氢钾氢氧化钠缓冲液（0.05M）X毫升0.2M K2PO4 + Y毫升0.2N NaOH加水稀释至29毫升 pH（20）X （毫升）Y（毫升）0.3720.5700.8601.26

7、01.7802.3652.9633.5003.9504.2804.5204.6809巴比妥钠-盐酸缓冲液（18）0.04M巴比妥钠溶液0.2V盐酸0.04M巴比妥钠溶液（毫升）0.2N盐酸（毫升）10018.417.816.713.411.479.397.218.48.89.09.49.65.213.822.521.651.130.70巴比妥钠盐分子量=206.18;0.04M溶液为8.25克/升10Tris盐酸缓冲液（0.05M，25）50毫升0.1M三羟甲基氨基甲烷（Tris）溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升。X（毫升）7.207.307.407.507.607.707

8、.8045.744.743.442.040.338.536.634.532.029.28.108.308.508.708.9026.222.919.914.712.4三羟甲基氨基甲烷（Tris）HOCH2 CH2OH CHOCH2 NH2分子量=121.14;1. 0. 1M溶液为12.114克/升。Tris溶液可从空气中吸收二氧化碳，使用时注意将瓶盖严。11硼酸硼砂缓冲液（0.2M硼酸根）0.05M硼砂（毫升）0.2M硼砂（毫升）0.2M硼酸（毫升）1.01.58.73.54.5硼砂Na2B4O7H2O,分子量=381.43;0.05M溶液（=0.2M硼酸根）含19.07克/升。硼酸H2BO

9、3,分子量=61.84,0.2M溶液为12.37克/升。硼砂易失去结晶水，必须在带塞的瓶中保存。11硼酸-硼砂缓冲液（0.2M硼酸根）10H2O,分子量=381.43;硼酸H2BO3，分子量=61.84, 0.2M溶液为12.37克/升。硼砂 易失去结晶水，必须在带塞的瓶中保存。12甘氨酸氢氧化钠缓冲液（0.05M）X毫升0.2M甘氨酸+Y毫升0.2NnaOH加水稀释至200毫升12.09.810.410.622.427.238.645.5甘氨酸分子量=75.07;0.2M溶液含15.01克/升。13硼砂-氢氧化钠缓冲液（0.05M硼酸根）X毫升0.05M硼砂+Y毫升0.2NNaOH加水稀释至200毫升9.311.010.134.043.046.00.05M溶液为19.07克/升。14碳酸钠-碳酸氢钠缓冲液（0.1M）Ca2+、Mg2+存在时不得使用0.1MNa2CO3（毫升）0.1MN2HCO3（毫升）20379.169.519.7810.2810.5310.838.779.129.7210.0810.5712346789Na2CO210H2O分子量=286.2;0.1M溶液为28.62克/升。N2HCO3分子量=84.0;0.1M溶液为8.40克/升。15“PBS”缓冲液PHNaClNaH2PO410000.10.20.3100ml8.5g2.2g0.4g