蛋白质相关实验技术操作指南.doc

1、1、Western blotting:Western blotting using ECL reagent used by：Laboratory of P.J. Hansen,Dept. of Animal Sciences, University of Florida Kampss Western Blotting Protocolused by：Sefton Lab, Salk InstituteDry Transfer 干法蛋白转膜used by：The Preuss Lab,The Division of Biological Sciences,The University of Ch

2、icago Western Blot of TBP from TBP-GST bacteriaused by：Steven Finkbeiner, Departments of Neurology and Physiology, UCSFWestern Blot/Anti-P-CREBused by：Steven Finkbeiner, Departments of Neurology and Physiology, UCSFWestern blotting protocol for 1C2, 3B5H10, and 4C8 Antibodiesused by：Steven Finkbeine

3、r, Departments of Neurology and Physiology, UCSFProtocol for anti-HA antibody Western Blottingused by：Steven Finkbeiner, Departments of Neurology and Physiology, UCSFProbing and Stripping of Western Blotused by：Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical InstituteWe

4、stern Blotting Protocolused by：Mirmira Laboratory at the University of VirginiaWestern Blotting and Immunostainingused by：Chazin Lab, Center for Structural Biology, Vanderbilt University Western Blotsused by：Lab of Dr. Mark Barton Frank, Oklahoma Medical Research FoundationWestern Blotting - Antibod

5、iesused by：The Minion Lab, College of Veterinary Medicine at Iowa State UniversityWestern Blottingused by：Cepko/Tabin Lab, Harvard UniversityCarbonate Solution For Western Blotused by：Dr. DE Koshland, Carnegie Institution of WashingtonWestern Blot with BSAused by：Dr. DE Koshland, Carnegie Institutio

6、n of WashingtonMETHOD for Western Blotsused by：Dr. DE Koshland, Carnegie Institution of WashingtonWestern Blotsused by：Michael Koelles Lab,Department of Molecular Biophysics and BiochemistryYale University2、蛋白分离/提取/浓缩 Lysis Buffersused by：Steven Finkbeiner, Departments of Neurology and Physiology, U

7、CSFYeast Protein Isolation Yaffe-Schatzused by：Amberg Lab ,Upstate Medical UniversityGuanidine Hydrochloride Purification of Proteins From SDS PAGEused by：Gottschling Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical InstituteYeast protein prep for SDS PAGE and western (rapid)

8、used by：Gottschling Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical InstituteYeast protein prep for SDS PAGE and western (glass bead)used by：Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical InstitutePurification of TFIIIC from yeastused by：Hahn L

9、ab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical InstitutePurification of TFIIA from E. coliused by：Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical InstituteTCA protein precipitationused by：Hahn Lab，The Fred Hutchinson Cancer Research Center and H

10、oward Hughes Medical InstitutePurification of MLCK ( Myosin Light Chain Kinase )from Rabbit Skeletal Muscle肌球蛋白轻链激酶used by：Stull Lab,University of Texas, Southwestern Medical Center Purification of Calmodulin from Bovine Brainused by：Stull Lab,University of Texas, Southwestern Medical CenterMyosin L

11、ight Chain Preparation from Skeletal and Cardiac Muscleused by：Stull Lab,University of Texas, Southwestern Medical CenterPurifying Protein from Inclusion Bodiesused by：Chazin Lab, Center for Structural Biology, Vanderbilt UniversityProtocol for Protein Extraction from Plantused by：The University of

12、Nebraska-Lincoln Protein Core Facility (PCF)Taq Polymerase Purificationused by：Cepko/Tabin Lab, Harvard UniversityYeast Protein Prep (No Boil)used by：Dr. DE Koshland, Carnegie Institution of WashingtonYeast Protein Prep (Boil)used by：Dr. DE Koshland, Carnegie Institution of WashingtonGST Fusion Prot

13、ein Prepused by：Vesicle Trafficking, Stanford University3、蛋白质检测分析：AMIDO BLACK STAINused by：Hancock Laboratory Methods,Department of Microbiology and Immunology,University of British Columbia, British Columbia, CanadaJames Hardwicks angiotensin assay protocolused by：Sefton Lab,Salk Institute Phosphoa

14、mino acid analysis:Mark Kampss methodused by：Sefton Lab,Salk InstituteULTIMATE HIS-UB ASSAY FORused by：the Tansey Lab at Cold Spring Harbor LaboratoryULTIMATE HIS-UB ASSAY FOR YEASTused by：the Tansey Lab at Cold Spring Harbor LaboratoryProtocol for Beta-Hexosaminidase RBL-2H3 Secretion Assayused by：

15、meyer lab,Stanford UniversityMeasurement of Myosin Regulatory Light Chain (RLC) Phosphorylationused by：Stull Lab,University of Texas, Southwestern Medical CenterBicinchoninic Acid (BCA) Protein Assayused by：David R. Caprette, Rice UniversityBiuret Protein Assayused by：David R. Caprette, Rice UniversityBradford protein assayused by：David R. Caprette, Rice UniversityHartree-Lowry and Modified Lowry Protein Assaysused by：David R. Caprette, Rice UniversityColorimetric Assaysused by：David R. Caprette, Rice UniversityKd measurement, fitting, calculation, and simulationused by：Chazi