生物专业英语综述作业Word格式文档下载.docx

1、 Now, go back and have a look at the way we have go throught, but find with love. To beginning with the fear and then relieved,but end with love. English for Biology Students brought me a lot of fun, sentiment and help. Teacher Zhang not only give us the professional English knowledge, whats more, h

2、e also gave us a lot of knowledge those outside of textbooks. He is an excellent professional teacher,He made me understand though excellent professional level is important，comprehensive ability and broaden our knowledge is indispensable.His many rich in philosophy, still so vivid in my mind! “Summa

3、ry or the abbreviation choose one from the two to do as last job about the Biological professional English. Originally I thought: abbreviation. Read an English literature, and then a little understanding, abbreviations would not be a problem. However, I still want to challenge myself. Not seeking co

4、mpared with others, but to surpass myself! So decisively I choose a larger review of the risk-summary . Fortunately, sophomore year, I served as the Minister of Science and Technology of Life Science, I have archived papers of all research projects. Of course, these papers of aresearch projects I wo

5、uldnt rumor. I only reference them to practice my English skills. The site and the mechanism of oxidation of Arginine kinaseAbstract Arginine kinase （arginine kinase, AK） （EC2.7.3.3） is a phosphodiesterase guanidines kinase, composed of 403 amino acids, which contains five cysteines （Cys23, Cys127,

6、Cys139, Cys201, Cys271）, the experiment we found that the oxidation state band and restore the state with the phenomenon of electrophoresis AK, and through a series of biotechnology means, respectively, of these five sites Cys mutated to serine （Ser）, to identify oxidation sites and to study the oxi

7、dation mechanism, and by UV fluorescence, respectively, to explore the structure after the mutation of the the AK various sites related to activity.Key words: Arginine kinase, rite-directed mutation, oxidation sites, SDS-PAGEIntroduction1.Arginine kinase1 （Arginine kinase AK） （EC2.7.3.3） is a kinase

8、 of a phosphorylated original guanidino compound exists widely in invertebrates, and is an important kinase that has a direct relationship with intracellular energy operation, muscle contraction, ATP-regenerating . Its Function is how to Catalytic reversible reactions by the following step: ATP phos

9、phate group is transferred to the arginine, thereby forming a high energy bond energy storage molecules - arginine phosphate. The reaction equation is as follows:Mg2 + ATP + Arg Mg2 + ADP + Arginine phosphate early in 1998, Mr Zhou with some people have used the crystal structure of the transition s

10、tate AK from horseshoe crabs by horseshoe to crab the Limulus polyphemus single subunit AK dissociation combined transition state analogs. The results showed that the AK was made up by a small -helix of the N-terminal domain and a large C-terminal structure （112, -357 residues）. The C-terminal domai

11、n is Similar to the C-terminal domain of glutamate synthase, the the Bagu antiparallel -sheet is around by seven -helical package 2. Seen from this structure, the transition state analog is not incorporated in the middle of the two domains, but combined in the C-terminal s Interior region, which mea

12、ns that the AK is the active site in the C terminal. In addition, the C-terminal domain can be divided into two small structure, while a transition state analogs is incorporated at the junction of the two domains 3. According to Mr. Zou Chenglus point of view that the active site of the enzyme is in

13、 a relatively flexible portion and is the parts of the structure ,and this Position is more susceptible from the impact of adverse external factors than other parts , the result is that the enzyme activity loss faster and easier than the enzyme of the whole structure .2 .The oxidation of protein Dif

14、ferent types of oxidative damage in protein is an important facter of the cause of many serious diseases （such as: cancer, diabetes, neurodegenerative diseases） The chaperone protein oxidative damage of ER , will lead to the misfolding and aggregation diseases in protein, such as: AD, Parkinsons dis

15、ease （Parkinsons disease, PD）, amyotrophic lateral sclerosis （amyotrophic of lateral sclerosis, ALS）, and Hang extension Raton chorea （Huntingtonsdisease, HD）, and senescence-related diseases, an important common feature of them is the abnormal protein in the brain within the accumulation. In the experiment of electrophoretic detection with AK we found the oxidation state band and reduced state band .Revelated by the research methods of creatine kinase （CK）, we make 5 sites Cys of AK mutated to serine （Ser）, then use ofaffinity chromatography, UV, fluorescence, such as a ran